Objective To assess patient ’ s fallopian tube function after laparoscopic salpingotomy for tubal ectopic pregnancy.Methods We observed the degree of pelvic adhesion and previous tubal patency by laparoscopic secondary exploration. The fallopian tube function were evaluated in 24 cases ( 26 fallopian tubes ) which had experienced laparoscopic salpingotomy. Results There were 4 cases of severe adhesions (16.7%), 4 cases of moderate adhesions (16.7%), 5 cases of mild adhesions (20.8%), and 11 cases of minor adhesions (45.8%).The status of fallopian tubes:tubal rapture and tissue absence were seen in 11 oviducts (42.3%), intact but obviously shortened oviduct with distal hydrops was seen in 11 oviducts (42.3%), and normal oviduct was recorded in 4 oviducts (15.4%).The methylene blue flow test was performed in the 26 tubes, showing 23 (88.5%) occluded and 3(11.5%) patent. Conclusions The fallopian tubes have incomplete shapes after laparoscopic tubal fenestration for ectopic pregnancy in most patients.We confirm that laparoscopic salpingotomy can not preserve the fertility effectively.

With the development of modern medical technology,the traditional teaching mode can not satisfy the teaching requirements of biomedical measurement and instrument which is one of the main courses of biomedical engineering,and network teaching is the tendency.The article studies how to develop biomedical measurement and instrument's network teaching from many aspects,such as the construction of information platform,network teaching pattern design and so on,and the practice has achieved good results.

The research of sleep staging is not only a basis of diagnosing sleep related diseases but also the precondition of evaluating sleep quality, and has important clinical significance. In recent years, the research of automatic sleep staging based on computer has become a hot spot and got some achievements. The basic knowledge of sleep staging and electroencephalogram (EEG) is introduced in this paper. Then, feature extraction and pattern recognition, two key technologies for automatic sleep staging, are discussed in detail. Wavelet transform and Hilbert-Huang transform, two methods for feature extraction, are compared. Artificial neural network and support vector machine (SVM), two methods for pattern recognition are discussed. In the end, the research status of this field is summarized, and development trends of next phase are pointed out.
Electroencephalography ; Humans ; Neural Networks (Computer) ; Sleep Stages ; Support Vector Machine ; Wavelet Analysis

ABRO1 (Abraxas Brother 1), known also as KIAA0157 or FAM175B, is an important component of BRISC deubiquitinating enzyme ( DUB) complex which specifically shears K-63 linked polyubiquitin chains .This article reviews the research progress in ABRO 1 in terms of its structural characteristics , BRISC complex composition , the regulation of DUB activity and interferon response , oxidative stress response and anti-myocardial ischemia and provides new ideas on fur-ther study of the physiological function of ABRO 1 and its association with immune and cardiovascular diseases .

[Summary] The human thyroid epithelium cells were obtained from normal para-adenoma tissues of patients with thyroid adenoma or nodule.Cells were treated with 1 000 IU/ml interferon-γ (IFN-γ) + 10 ng/ml tumor necrosis factor-α (TNF-α),1 mmol/L N-acetylcysteine (NAC),1 mmol/L glutathione (GSH),and 10 μmol/L dexamethasone (DEX) respectively.Malondialdehyde(MDA),glutathioneperoxidase(GSH-Px),and superoxidedismutase(SOD) levels in the cell supernatant were measured.The results showed that IFN-γ+TNF-α significantly increased MDA level (P＜0.05) while decreased GSH-Px and SOD levels (P＜0.05).After NAC,GSH,and DEX intervention,MDA levels all were significantly lowered (all P＜0.05) while GSH-Px and SOD levels were significantly increased compared with IFN-γ+TNF-α stimulation(all P＜0.05).These results suggest that IFN-γ and TNF-α can induce oxidative stress in the human thyrocyte,and this effect is antagonized by NAC,GSH,and DEX via increasing GSH-Px,SOD activity and decreasing MDA content.

Objective To investigate the association of serum ferritin and hemoglobin with the pathogenesis of primary restless legs syndrome(RLS). Methods Thirty-five patients with primary restless legs syndrome and twenty insomnia controls were included in this study. The level of serum ferritin and hemoglobin were measured, and the data have been analyzed using t-test. Results The level of serum ferritin in the primary RLS patients (89.77μg/L (SD:48.52)) was significantly lower than in the contruls (123.36 μg/L (SD:35.06)) (t=-2.713,P <0.01), whereas the level of hemoglobin have no statistical difference between the RLS group (142.77 g/L (SD: 11.79)) and the control group (139.05 g/L (SD: 12.33)) (t = 1.108, P > 0.05). Conclu-sions The decrease of serum ferritin may be a risk factor of primary restless legs syndrome, but the level of hemo-globin is not associated with primary restless legs syndrome.

OBJECTIVE:To evaluate the cost and effectiveness of two chemotherapy regimens in the treatment of initially di-agnosed multiple myeloma (MM). METHODS:63 patients who were newly diagnosed as MM without transplant chance in our hospital were analyzed retrospectively,and then divided into MPT group (alkeran+metacortandracin+thalidomide,41 cases) and VDT group (bortezomib +dexamethasone+thalidomide,22 cases) according to chemotherapy regimens. Clinical efficacy,survival time and ADR of 2 groups were observed,and cost-effectiveness analysis of them were conducted. RESULTS:The overall re-sponse rates of MPT group and VDT group were 63.4% and 77.3%,respectively(P=0.260);the complete response rate of them were 12.2% and 36.4%,with statistical difference(P=0.024). The progression free survival of MPT group and VDT group were (14.314±0.488)months and(18.557±0.848)months,with statistical significance(P<0.001);the overall survival of MPT group and VDT group were(33.536±1.143)months and(41.048±1.868)months,with statistical significance(P=0.007). Leukocytope-nia,nausea and vomit and peripheral neurotoxicity of VDT group were both higher than those of MPT group,with statistical signif-icance (P<0.05). The cost-effectiveness ratio of MPT group was 247.48,and that of VDT group was 2 922.77;the incremental cost-effectiveness ratio was 15 125.18. The results were consistent with the sensitivity test. CONCLUSIONS:Clinical efficacy and survival time of VDT regimen is better than that of MPT regimen,but MPT regimen is superior to VDT regimen in respect of cost-effectiveness analysis and the incidence of ADR. which will more suitable for the MM patients without transplant chance or goood economic conditions.

Graves′disease ( GD) is an organ-specific、autoimmune and hyperthyroid thyroid disease closely related to the TSH receptor antibody ( TRAb) .Oxygen free radicals and the oxidative damage caused by these are closely related to the development and progression for GD .Researches on oxidative stress are providing novel therapeutic targets of GD .

To investigate the protective effects and possible mechanism of Mycelium of Hirsutella hepiali Chen et Shen (MHCS) on metabolic syndromes, free fatty acid and MHCS-treated hepatocytes were used for detecting autophagy-related LC3, p62 and lipid accumulation. Moreover, high fat diet fed mice were used to establish metabolic syndromes model. 50-weeks age mice were randomly divided into: control group, model group and MHCS group. At 80-weeks age, 15 mice were randomly chosen from each group separately for examining oral glucose tolerance, serum insulin, insulin-like growth factor 1 (IGF-1), hepatic LC3, p62, p-NF-kappaB p65, NF-kappaB p65, IL-6 and CXCL-8. Moreover, insulin resistance index (IRI) was calculated. Hepatic pathological changes, including vacuoles, lipids accumulation and fibrosis were observed. Remaining mice were fed with diet separately to 110 weeks-age for statistics of mortality. MHCS promoted autophagy of free fatty acid treated hepatocytes. Mice fed with high fat plus MHCS diet exhibited improved oral glucose tolerance, insulin resistance, hepatic pathology, inflammation, mortality and activated autophagy. The protective effects of MHCS against metabolic syndroms might be through the activation of hepatic autophagy.

Objective To discuss the clinical significance of vancomycin plasma concentration monitoring and its relationship with adverse reactions in treatment of elderly patients. Methods The clinical records of 32 patients aged over 70 years admitted in the Emergency Medical Department of Sichuan Provincial People's Hospital were collected. Based on the diagnosis and laboratory examinations on admission, the patients were treated with cefoperazone+sulbactam or moxifloxacin, while in patients with severe infections, intravenous drip of carbapenem antibacterial drugs were given. After treatment for 3-5 days, when no effective results were obtained, according to the pathogenic results achieved from cultures of blood, sputum, secretions, catheter, etc, the corresponding treatment was given. If the infection was caused by positive bacteria mainly methicillin-resistant Staphylococcus aureus (MRSA) sensitive to vancomycin, the original antibiotic was replaced by vancomycin or vancomycin combined with other antibiotic; intravenous drip of vancomycin 1 g in 250 mL normal saline was given, once in 12 hours, with a speed of 10 mg/min or not over 15 mg/min. The function of liver and kidney, and auditory impairment were observed, and the correlation between vancomycin plasma concentration and adverse reactions was analyzed. Results The total incidence of adverse reactions in elderly patients with different serum vancomycin trough concentrations was 37.50%(12/32) in which the highest incidence was kidney damage 18.75%(6/32) followed by the hearing loss 9.38%(3/32) and liver damage 9.38%(3/32). There were no statistical significant differences in the rates of adverse reactions among the different vancomycin trough concentrations (<10, 10-20,>20 mg/L) and [37.50%(6/16), 38.46%(5/13), 33.33%(1/3), all P>0.05], that meant along with the increase of vancomycin concentration, no tendency of increment of adverse reactions was seen. Of the 32 cases, there were 13 treated by vancomycin combined with other antibiotic, the combination rate being 40.63%. There were 2 cases of hepatic impairment (11.83%), 1 case of renal impairment (7.69%) and none hearing damage in patients treated with combination of antibiotic therapy. Therefore no correlation was concluded between the occurrence of adverse reactions and the combination therapy ( r=0.15, P>0.05). Conclusions In elderly patients over the age of 70 years, there is a higher incidence of kidney damage in the application of vancomycin. Thus, the monitoring of vancomycin plasma concentration in elderly patients has instructive significance in its clinical use.