Objective To design a pulse wave measurement system based on ZigBee wireless technology. Methods CC2430 controlled by the pulse sensor unit will be collected in pulsatile signal to human superficial artery through analog circuit preprocessing, which could be transformed into digital signal in digital model, and then can be impressed and displayed by control unit to deal with and display, at the same time, data in ZigBee wireless communication data unit is sent to the host computer and these data would be retrieval and playback of medical workers. Results The experiments show that the system is able to acquire the pulse wave of the human body in real-time, at the same time, through the ZigBee wireless network, it can be real-time, secure and reliable communications. Conclusion It is applicable to a wide range of health movement monitoring, monitoring of mobile hospitals and other areas.

Objective To design a wearable monitoring system for community medicine.Methods The wearable monitoring system was composed of wearable physiological signal detection component,wireless data transmission component and the central signal processing component,with new technologies adopted such as wearable detection technology and Zigbee wireless communication technology.Results The acquired signals were uploaded to the community medical monitoring terminal through Zigbee wireless network for signal processing,display and pre-diagnosis.The function of emergency alarm was also available.Conclusion The wearable monitoring system,with realtime signal acquisition and Zigbee wireless communication,can be applied to mobile monitoring during exercise or in hospital.

Calcium overload has been discovered in the ischemia and reperfusion injury of the heart recently, but there were less reports about changes in myocardial Ca distribution under this same condition by electronmicroscopy. Using the potassium-pyroantimonate method and X-ray microanalysis, we studied cytochemical calcium localization in the rabbit's myocardium at various time periods of ischmia with or without subsequent reperfusion. At the same time, we had the morphometric estimation of Ca granule volume density in the mitochondria by TAS(texture analysis system) and evaluated Danshen's protection for ischemia and-reperfusion injury. The results showed that mitochondrial Ca granule volume density increased significantly with the development of ischmia and post-ischemic reperfusion, especially during reperfusion following 40min of ischemia, however following 60min of ischemia, Ca granule volume density decreased than that observed following 40 min of ischemia. This meant that the limit time point for reversible and irreversible injury could be in the range of 40～60min of ischemia. Danshen pretreatment to the rabbits reduced Ca granule volume density significantly. This demostruted that Danshen acted as a calcium antagonist and contributed an outstanding protection to the myocardial calcium overload.

Objective The pathogenesis of T2DM has not been elucidated.This study aimed to explore the association of FXR gene polymorphisms with the risk of T2DM in Eastern China population. Methods We collected 467 cases in the Health Examination Center of our hospital from January 2011 to December 2012.Ligase detection reaction was performed to test the genotypes in 240 T2DM patients and 227 controls.Unconditioned logistic regression analysis was used to explore the association of FXR gene polymorphisms with the risk of T2DM in different genetic models, after adjusting for age, sex, smoking and drinking status. Results Compared with AA genotype, GG significantly increased the risk of T2DM (P=0.017), while GA+GG approached marginal significance in association with T2DM (P=0.049) in rs1030454.For rs10860598, GA reduced the incidence of T2DM (P=0.025) compared with AA genotype, while GA+GG had marginal significance with T2DM (P=0.049).For rs11110411, CT or CT+CC could reduce the risk of T2DM compared with TT with P value of 0.016 and 0.024 respectively.Compared with AA, GA or GA+GG in rs17030270 was negative with the risk of T2DM with P value of 0.018 and 0.032 respectively. Conclusion FXR gene polymorphisms are associated with the risk of T2DM in northern Chinese Han population and the variation of FXR gene may play a role in the pathogenesis of T2DM.

Objective To investigate relationships between the expression of Bcl-2,Bax protein and neuronal apoptosis in hippocampus of posttraumatic stress disorder(PTSD) rats.Methods The SPS-method was used to set up the rat PTSD models.There were five groups:after SPS 1d,4d,7d,14d groups and control group.Apoptotic cells were detected by electron microscopy and TUNEL method.The expressions of Bcl-2 and Bax proteins were detected with immunohistochemistry,double fluorescent,confocal laser scanning microscopy and Western blotting.Results Apoptotic cells were present in hippocampus of PTSD rats.The number of apoptotic cells increased with the development of PTSD and peaked on the 7th day after SPS,then decreased gradually.The expression of Bcl-2 protein peaked on the 4th day after SPS and Bax protein peaked on the 7th day after SPS,then decreased gradually.The ratio of Bcl-2/Bax increased at the beginning and then gradually decreased.Conclusion The neuronal apoptosis in hippocampus of PTSD rats may be one of reasons inducing hippocampus atrophy.The increase of Bcl-2 and Bax protein and the change of Bcl-2/Bax ratio would play an important role in regulating the hippocampal neuronal survival or death during posttraumatic stress disorder.

Objective To establish a method for the determination of Forsythin in Shenyan Jiere Tablets by HPLC. Methods The separation was performed on shim-pak clc-ODS (4.6 mm?150 mm, 5 ?m) with Accetonitrile-0.025 mol/L H3PO4 (20∶80) as mobile phase. The flow rate was 1 mL/min and UV detector was at 277 nm. Results The method had good average recovery with 97.1% (n=5), RSD=1.6%, good linear relationship within the range of 0.12～0.5 ?g. Conclusion The method is accurate, reliable and can be used for quality control of the production.

0 05). It is concluded that the determination of CD 44V6 expression might be useful in assessing breast cancer progression and lymphatic metastasis

Objective Changes of thyroid stimulating antibody(TSAb) and thyroid stimulating blocking antibody(TSBAb) in the treatment of anti-thyroid drugs(ATDs), and the effect of ATDs combining with levothyrocine(LT4) on TSAb and TSBAb were analyzed. Methods Using recombinant Trxfus. TSHRn protein and Trxfus. TSHRc protein as antigens, and TSH receptor antibody(TRAb)-N(TSAb binding hot spots), TRAb-C(TSBAb binding hot spots)in the serum of thyroid disease patients were measured with ELISA. The changes of TRAb-N, TRAb-C over 36 months in 117 TRAb-N positive Graves′ patients with hyperthyroidism were analyzed retrospectively. In the course of treatment, 41 cases as A group with ATDs and LT4 treatment, 76 cases as B group with only ATDs, The changes of TRAb-N and TRAb-C were observed in the two groups. Results (1)According to the change of TRAb-N, 117 TRAb-N positive Graves′ patients with hyperthyroidism were different. In group Ⅰ, 10 patients continued to have persistently positive TSAb and continued to have hyperthyroidism, remission rate 0%. In group Ⅱ, 17 patients showed complicated TRAb-N changes, 12 of 17 patients got relapse, 5 of 17 patients got remission, remission rate 29.4%. And in group Ⅲ, with TRAb-N dropping gradually, 15 of 89 patients got relapse, 74 of the 89 patients got remission, remission rate 83.1%. Three groups were significantly different with x2 test(P<0.01). One of the 117 TRAb-N positive Graves′ patients with hyperthyroidism developed TRAb-C positive hypothyroidism. (2)According to combining with and without LT4 during the treatment of ATDs,the patients were divided into 2 groups(Group A: ATDs combined with LT4; Group B: only ATDs). These 2 groups were significantly different in TRAb-N at baseline and 3 months(P<0.01), TRAb-C between two groups were not significantly different in all times(P>0.05). Conclusion TSAb and TSBAb can be used to document TRAb-function, which is significant for us to predict the changes of thyroid function. During ATDs treatment, the temporary early low-dose application of LT4 did not significantly affect TSAb and TSBAb.

Objective To select and express a human thyrotrophin receptor antibody (TRAb) Fab fragment from phage antibody library constructed with phage display technology. Methods With immobilized antigen, the reconstructed humanized TRAb Fab library was enriched by five rounds panning (adsorption-elution-amplification). The TSAb Fab and TBAb Fab fragment were selected by coated fusion proteins of hTSHRn and hTSHRc. The positive clones were identified and selected by Phage-ELISA. TRAb positive clones were identified by PCR and double restriction enzyme digestion. The soluble TRAb (TSAb, TBAb) Fab fragments were expressed. TRAb (TSAb, TBAb) Fab fragments were identified by Western blotting assay. The DNA fragment was sequenced from the positive clones. Results Following five rounds of biopanning, TRAb (TSAb,TBAb) Fab phage antibody was screened. The enrichment effect reached to 77 times and 94 times. The soluble TRAb (TSAb,TBAb) Fab antibodies were expressed from positive clones and identified by phage ELISA. Western blotting analysis showed that the phage displaying Fab had significant binding activity with antigens. These sequence analysis showed that all of the heavy chain Fd gene and light chain gene were derived from human immunoglobulin variable region. The light chain variable region of the monoclonal 48 was homologous to the immunoglobulin light chain Vλ homology of 94.4%, and the heavy chain variable region of the monoclonal 48 was homologous to the immunoglobulin heavy Fd chain VH4 homology of 88.9%. The light chain variable region of the monoclonal 56 was homologous to the immunoglobulin light chain Vλ homology of 95.6%, and the heavy chain variable region of the monoclonal 56 was homologous to the immunoglobulin heavy Fd chain VH3 homology of 84.6%. Conclusion We have successfully selected TRAb (TSAb, TBAb) Fab fragment from a human phage display immune antibody library.