Objective To observe integrin linked kinase(ILK)expression in obstructive nephropathy with renal tubular acidosis, and investigate the function of renal tubular acidosis on renal interstitial fibrosis.Methods 75 rate were divided into control group and unilateral ureteral obstruction(unilateral ureteral obstruction(UUO,n=25)group,and other 50 rats in UUO group.UUO group was divided into renal tubular acidosis (UUO1 group,n=31)group and non-renal tubular acidosis(UUO2,n=19)group,re-nal function and histology test was carried at the same time.7,14,21,28 days later after operation,the rats were killed in batches, then measured the expression of ILK in renal tissue by immunohistochemistry and western blot.Results Inflammatory cell infiltra-tion and interstitial fibrosis of UUO1 group were more serious under the light microscope than that of UUO2 group;there was only a small amount of ILK protein expression in control group,almost no FN protein deposition,expression of ILK and FN protein pre-cipitation of UUO2 group increased,and UUO1 group was more than UUO2 group.Conclusion In obstructive nephropathy,renal tubular acidosis can up-regulate expression of ILK and FN sedimentation,then lead to interstitial fibrosis promotion.

Objective:To explore,on the basis of principles of bibliometrics,the distribution of the writers,institutions,regions and journals of the publications on HIFU and the literature amount,and to learn the situation of cross discipline study on HIFU and of the different regions where the articles on HIFU came in order to make a clear view of the characteristics and the status of co-operations in HIFU work and to determine,among them,the key authors,key institutes and key regions so that the progress of HIFU study was turned out.Method:Based on the information drawn from VIP-database,bibliometric method was used to analyze all the data on HIFU before the year of 2007.Result:833 articles were published before the year of 2007,in which 686(72.87%) were written by more than one authors,with a degree of collaboration as 2.78.The number of articles by mono-author was 312(37.45%).186 articles(22.33%) were from Chongqing,taking the first place in number.134 of the key authors published 410 papers(49.22%).10 of the institutes with more than 9 articles turned out 249 papers(29.89%).2000 saw an obvious change in the situation of publications on HIFU and the collaboration among the authors and from that year to 2006,792 papers were published with a degree of collaboration as 3.72 and the average rate of co-authership as 81.12%.Conclusion:The authors on HIFU,with a group as their key authors who were advanced in academy,productive in papers and influential on various journals in medical field,were widely distributed and had a high cooperative rate.But it was also revealed that the researchers in the field of HIFU were unevenly distributed,with the institutions in Chonqing taking the lead.

Objective To investigate the effects and clinical value of neoadjuvant chemotherapy in patients with locally advanced cervical cancer.Methods 102 cases with locally advanced cervical cancer treated were randomly divided into observation group and control group,the observation group received the surgery after the neoadjuvant chemotherapy,and the control group was given a direct surgical treatment.Operative time,blood loss and postoperative pathologic results were observed,and chemotherapy and toxicity evaluation were made for the observation group.Results The chemotherapy effective rate of the observation group was 74.51％,and the toxicity could be tolerated;the operation time and blood loss of the observation group were significantly lower than those of the control group(all P ＜ 0.05) ;surgical trauma and postoperative complications' occurrence rate of the observation group were significantly lower than those of the control group (all P ＜ 0.05) ; pelvic lymph node metastasis and vaginal cutting edge of the residues of the observation group were significantly lower than those of the control group (all P ＜ 0.05).Conclusion For locally advanced cervical cancer,NACT has a positive significance in reducing the tumor volume,low-er tumor stage,and at the same time reducing the difficulty of operation,surgical trauma,postoperative complications,and preventing recurrence and metastasis,which can be used as a better adjuvant treatment program for locally advanced cervical cancer.

Mesenehymal stem cells CMSCS are nonhematopoietie stromal cells that are capable of differentiating into, and contribute to the regeneration of, mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon and adipose. MSCs have been isolated from human first- and second-trimester fetal blood, liver, spleen, bone marrow, adipose tissue, amniotie fluid, periosteum and embryo. Not only can they express many phynotypes involving adhesion molecules and chemokine receptors, but also have character of immune tolerance, so they have potential of migration and transportation to target issue and feature of immune suppressing. There are wide application prospects for a number of areas in the cardiovascular, neurological, blood, surgical injury and other diseases. The current review describes the phynotypes, the immune tolerance and the potential for homing of MSCs.

Generally speaking,the hepatocyte stem cell is not a specific cell type.but an overall name of all kinds of cells that possess stem cell characters about embryonic development and regeneration of liver.HSC is a pluripotential stem cells which have self-renewal capacity and could differentiate into mature hepatocytes and bile duct cells.According to the different origin of hepatocyte stem cell,it can be usually divided into two kinds:non liver-derived hepatocyte stem cell and liver-derived hepatocyte stem cell.

Third-generation aromatase inhibitors (AIs) have been considered as standard adjuvant endocrine therapy for hor-mone-responsive breast cancer in postmenopausal women. Aromatase inhibitor-associated musculoskeletal symptoms (AIMSS) are prevalent among patients undergoing AI therapy;these symptoms result in reduced quality of life and poor dependence to AI treatment. The mechanisms mediating AIMSS and the effective management of AIMSS have not been extensively investigated. This article re-views the incidence, clinical characteristics, possible mechanisms, and treatment of AIMSS.

[Objective] To study the best skill of HP-?-CD containing Ezhu oil.[Method] Take Ezhu oil containting rate as appraisal index,use orthogonal testing method for technical selection.[Result] Apply water solution mix for inclusion,the gotten best technical conditions:under 60 ℃,Ezhu oil:HP-?-CD=1:30,the regulating rotating speed 600 r/min/5 h;the total score of inclusion rate and recepting rate was 90.97%.[Conclusion] The preparation technical impoves stability of Ezhu oil,with better optimization,and offering realiable theoretic foundation for industrial production.

Objective: To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with cation exchange-based solid phase extraction (SPE) for determination of tetrodotoxin (TTX) in salted pufferfish. Methods: Evenly crushed salted pufferfish samples were subjected to ultrasound-assisted extraction with 0.5% acetic acid/50% methanol/water. The extract was cleaned with cation exchange-based SPE cartridge and eluted with 0.3% hydrochloric acid and 50% acetonitrile/water. The eluent was neutralized with ammonia and separated with a Waters XBridgeTM BEH Amide column (150 mm×3.0 mm, 1.7 μm), and determined using LC-MS/MS in a multiple reaction monitoring mode. Results: The matrix effects of TTX were 85.7%-92.4%, and the matrix suppression effect was under effective control following clean-up procedures using the optimized SPE method. The TTX showed a good linear relationship at the range of 2.0 to 4 000 μg/kg, with a correlation coefficient (r2) of 0.999 2. The limits of detection and quantitation for TTX in sample matrix were 1.0 μg/kg and 2.0 μg/kg, respectively. The mean spiked-recovery rates were 81.2% to 96.5% at spiked amounts of 2.0, 200 μg/kg and 2 200 μg/kg, with relative standard deviations (RSDs) of 4.3% to 7.5%. The intraday accuracy and precision of TTX were 84.4% to 95.6% and 4.9% to 5.8% in quality control samples, and the interday accuracy and precision of TTX were 86.1% to 94.9% and 5.5% to 8.5% in quality control samples. The detection of TTX was 60.5% in 38 market-sold salted pufferfish products using the established LC-MS/MS method. Conclusion The established LC-MS/MS method is effective for accurate quantitative determination of TTX in salted pufferfish.

Objective:To investigate the changes of pulmonary IMD and its receptor system - calcitonin receptor-like receptor (CL) and receptor activity modifying proteins (RAMPs) mRNA in acute lung injury(ALI) induced by oleic acid of rats. Methods: Contents of IMD in plasma and lung homogenates were measured by radioimmunoassay(RIA). The lung mRNA of IMD, CL and RAMPs was determined by semi-quantitative RT-PCR. Results: Compared with control group, in ALI group, the contents of IMD_ 1-53 in plasma and lung homogenates were decreased by 20.8% and 74.5% (all P

Objective To isolate and indentify fetal hepatic progenitor/stem cells, and study the feasibility and effectiveness of their transplantation on acute liver injury in nude mice. Methods The primitive cells isolated from 13.5dpc pregnant mouse fetal hver by way of enzyme digesting were cultured in vitro and liver specific markers as AFP, CK19, Albumin, c-Met, were identified by immunofluorescence and RT-PCR on colonies. 4 × 105 cells were transplanted into nude mice with carbon tetrachloride induced acute liver injury. Hapatic functions were measured pre- and -post transplantation on days 1,2,4, 7. Meanwhile, hepatic pathology was studied. Results Compared to control group the hepatic functions gradually recovered in transplant group, on days 1,2,4 after fetal hepatic stem cell transplantation. The hepatic pathology significantly improved in stem cells transplantation group. Conclution Fetal hepatic progenitor/stem cell were successfully yielded by enzyme digest. Stem cells transplantation improved the hepatic function and pathology in acute hapatic injury model of nude mice.