Objective To investigate the changes and clinical significance of calcitonin gene related peptide (CGRP) and interleu-kin 1α(IL-1α) before and after warm acupuncture therapy in the patients with lumbar disc herniation (LDH ) .Methods Serum CGRP and IL-1αcontents were detected before and after the warm acupuncture therapy in 60 cases of lumbar disc herniation and 15 healthy control cases by ELISA method for conducting the statistical analysis .The changes of the McGill pain scores and serum CGRP and IL-1αconcentrations before and after the warm acupuncture therapy were observed .Results The McGill pain scores af-ter the warm acupuncture therapy in the LDH patients were significantly decreased ,the difference was statistically significant (P<0 .01) ,and serum CGRP and IL-1α concentrations were significantly decreased ,the difference was statistically significant (P<0 .01) .Conclusion CGRP and IL-1αcan be used as the observation index of the effects for the warm acupuncture therapy in trea-ting LDH ,which can provide the basis for the conservative treatment of LDH .

Objective:To evaluate the effect of miR-133b on the apoptosis and radiosensitivity of colon cancer cell line (SW620 cells), and to explore its mechanism.Methods:SW620 cells were transfected with miR-con (miR-con group), miR-133b mimics (miR-133b group), si-con (si-con group) and si-HER-2(si-HER-2 group) by the liposome method, and then irradiated with 0, 2, 4, 6, 8 Gy. The miR-133b protein expression, HER-2 protein expression, apoptosis, cell survival fraction and cytofluoroactivity in each group were evaluated by qRT-PCR, Western blot, flow cytometry, colony formation assay and dual luciferase reporter gene assay, respectively.Results:Compared with the pre-irradiation group, the expression level of miR-133b was significantly down-regulated ( P<0.05), whereas that of HER-2 was significantly up-regulated in SW620 cells after irradiation ( P<0.05). Overexpression of miR-133b and knockdown of HER-2 remarkably reduced the survival fraction (both P<0.05), and significantly promoted the apoptosis of SW620 cells ( P<0.05). miR-133b could considerably inhibit the fluorescent activity of wild-type HER-2 cells ( P<0.05) and negatively regulate the expression of HER-2 protein. Conclusion:miR-133b can inhibit the survival of colon cancer cells, promote the apoptosis and enhance the sensitivity of radiotherapy probably via the mechanism of targeting HER-2.

Objective To analyze the Epstein-Barr virus(EBV) infection status in children .Methods The EBV infection related antibody data of the children patients in our center during 2013 were collected and performed the statistical analysis on the age ,dis-ease type and month distribution .Results Totally 7 582 children cases were detected .The total infection rate of EBVCA-IgM was 16 .97% .The positive rates of specific EBVCA-IgM in different age stages were 15 .92% in 0 - <3 years old ,22 .53% in 3 - <6 years old ,16 .69% in 6- <10 years old and 8 .30% in more than 10 years old;in EBV infection cases ,most of them were infections mononucleosis ,followed by respiratory tract infection ;March and September had the highest EBV infection rate .The positive rates of series antibodies detection of EBV were 19 .11% for EBVCA-IgM ,61 .57% for EBVCA-IgG ,53 .76% for EBNA-1-IgG and 9 .50% for EBVEA-IgG ,the proportion of the combination of positive EBVCA-IgG and positive EBNA-1-IgG was highest ,account-ing for 39 .57% .Conclusion EBV is an important infectious pathogen in children infection ;39 .57% of children are once infected by EBV in the past ;with the age increase ,the EBV infection proportion will be decreased .

BACKGROUND: Puerarin, the main effective component of Chinese herb, Radix puerariae, is isoflavone monomer, which can counteract learning and memory impairment induced by scopolamine or D-galactose etc.OBJECTIVE: To investigate the protective effects of puerarin on β-amYloid peptide-induced learning and memory impairment of model mouse of dementia and the changes of superoxide dismutase activity and malondialdhehyde content in brain and blood.DESIGN: Randomized controlled trailSETTING: Department of Pharmacology, Capital University of MedicalSciencesMATERIALS: The experiment was conducted in Departmentof Pharmacology of Capital University of Medical Sciences from March to June 2002.A total of 40 ICR mice were selected and randomly divided into 4 groups:pseudooperation group, dementia model group, puerarin 25 mg/kg group and puerarin 50 mg/kg group, with 10 in each group.METHODS: ①Model preparation: After anaesthesia with pentobarbital sodium, single intraventricular injection of 3 μL β-amyloid peptide was conducted from right side on each mouse in dementia model group, puerarin 25 mg/kg group and puerarin 50 mg/kg group under aseptic manipulation. The same operation was carried out on the mouse in pseudooperation group but without injection of β-amyloid peptide. ②Giving medicine:10 mL/kg physiological saline was intraperitoneally injected into the mouse in pseudooperation group and model group; 25 mL/kg puerarin was intraperitoneally injected to the mouse in 25 mg/kg puerarin group; 50 mL/kg puerarin was intraperitoneally injected to the mouse in 50 mg/kg puerarin group.The medicines were given to each group from the day of model preparation on and behavioral test was carried out 12 days later. ③ Morris water maze examination was used to detect learning and memory ability of the mice.Time for finding the platform (escape latency) in 2 minutes, swimming distance, original angle and search strategy were recorded as learning results.④When the above experiment was finished, anaesthesia with ether was applied to the mice and blood was collected from the orbit to prepare serum.After that, the mice were put to death by decapitation and the tissue of right-brain of the mice were rapidly took out to prepare cerebral homogenate in ice bath, then superoxide dismutase activity and malondialdhehyde content were determined in brain and serum.MAIN OUTCOME MEASURES: ①Escape latency, swimming distance,search strategy and original angle for the mouse in each group to reach the latform. ②Superoxide dismutase activity and malondialdhehyde content in brain and blood of the mouse in each group.RESULTS: All the 40 mice were involved in result analysis. ① Escape latency and swimming distance were shortened in puerarin 25 mg/kg and 50 mg/kg groups (P ＜ 0.05-0.01). The results of search strategy and original angle indicated that as the number of training days increased, the frequency of randomization+magin strategy gradually decreased; The decreasing rates and extents in pseudooperation group and puerarin 25 mg/kg and 50 mg/kg puerarin were more rapid than that in dementia model group,meanwhile, the increasing rates and extents of frequency of tendency+directness strategy in these groups were more rapid than that in dementia model group. There was no significant difference in original angle between groups (P ＞ 0.05). ② The content of superoxide dismutase increased and that of malondialdhehyde decreased in brain and blood of the model mouse in puerarin 25 mg/kg group andpuerarin 50mg/kg group (P ＜ 0.05 -0.01).CONCLUSION: Puerarin can counteract the neurotoxicity of β-amyloid peptide, which improves learning and memory of model mouse. It is not relevant to the dosage but probably related with elimination of cerebral free radical and improvement of antioxidation activity.

AIM　The effect of bifico on experimental hepatic encephalopathy (HE) induced by thioacetamide(TAA) and the possible mechanism of its protective effect were studied. METHODS　Experimental hepatic encephalopathy was induced by ig thioacetamide 250 mg*kg-1 successively for two days; bifico was administraed ig once per day for one week before HE induction. RESULTS　Compared with control HE rat, Bifico improved rat neuro-reflexes score and hepatic injury grade(P<0.05); thus decreased rat serum ammonium and LPS concentrations, respectively (P<0.05,P<0.01). The preparation slightly increased the ratio of chain amino acid to aromatic amino acid as well as reduced the degree of liver necrosis. CONCLUSION　At the dosage of 840 mg*kg-1 ig for one week before liver intoxication, Bifico significantly protects rats from hepatic encephalopathy induced by TAA.

Objective To investigate the rs501120 and rs17465637 gene polymorphisms,and their relationship with the risk of coronary heart disease(CHD)in Chinese Han population.Methods 775 CHD without treatment and 775 age and gender matched controls were selected for this study,the genotypes of two single nucleotide polymorphisms(SNP)rs501120 and rs17465637 were tested with TaqMan-MGB probes.Results There was no significant difference in the frequency of genotypes of the 2 SNPs between CHD group and control group(P ＞0.05).Stratified analysis showed that SNP rs501120 had significant protection with CHD in people younger than 60 years old(OR 0.4,95% CI 0.2-0.9,P ＜ 0.05)or people with diabetes(OR0.3,95%CI0.1-0.7,P ＜0.05).Conclusions The results suggested that rs501120 was tightly associated with CHD in people younger than 60 years or had diabetes.

Aim To study the characteristics of the binding reaction of Troxetutin with bovine serum albumin (BSA) by fluorescence and ultra violet-visible absorption spectra.Methods The quenching mechanism of the fluorescence of BSA by troxerutin was studied with fluorescence.To determine the dynamic quenching constants and static binding constants,the Stern-Volmer equation and the double reciprocal Lineweaver-Burk equation were applied. The number of binding site was calculated with double logarithmic equation and the main binding force was discussed by thermodynamic equations. The binding distance and energy transfer efficiency between donor (BSA) and acceptor (troxerutin) were obtained effectively quenched fluorescence of BSA via static quenching processes. The binding constant Ka was calculated to be in the order of 106,indicating a strong interaction between Troxerutin and BSA. The number of binding site was approximately equal to 1,the binding distance was 1.97 nm,the energy transfer efficiency was 0.529,and the binding force was mainly hydrophobic force.Conclusion Troxerutin effectively quenchs the intrinsic fluorescence of BSA via static quenching mechanism,and the binding is mainly driven by the hydrophobic interaction.

Objective To investigate the accumulation and maturation status of pulmonary conventional dendritic cells (cDCs) in the early phase of acute lung injury (ALI),and to explore the way of the inflammatory responses and lung injury modulated by cDCs in vivo.MethodsMale C57BL/6 mice were randomly ( random number) divided into the normal control group,6 h-ALI group and 24 h-ALI group.Murine model of ALI was made by intra-tracheal administration of lipopolysaccharide (LPS) and lung specimens were taken 6 h or 24 h later.The accumulation and maturation status of pulmonary cDCs were assessed by flow cytometry.IL-6 and TNF-α were quantified to evaluate the lung inflammation.Transcription factors T-bet/GATA-3 mRNA ratio was determined to estimate the balance between Th1/Th2 responses.IFN-γand IL-4 were quantified to evaluate Thl-specific and Th2-specific cytokine production respectively.Lung injury was estimated by lung wet weight/body weight ratio (LWW/BW) and histopathological assessment.Comparison between groups was performed using one -way ANOVA.ResultsCompared with normal control group,LPS challenge resulted in higher level of IL-6 and TNF-α,increased LWW/BW ratio and significant histopathological changes (P ＜0.01 ).The accumulation and maturation of pulmonary cDCs in 6 h-ALI group were significantly increased after LPS challenge (P ＜0.01 ),while the accumulation and maturation of pulmonary cDCs in 24 h-ALI group were significantly lower than that in 6 h-ALI group ( P ＜0.01 ).Compared with normal control group,the expression of T-bet mRNA in 24 h-ALI group was markedly enhanced ( P ＜ 0.01 ) and the production of IFN-γ was increased as well ( P ＜ 0.01 ).ConclusionsThe accumulation and maturation of pulmonary cDCs peaked within 24 h after LPS challenge,pulmonary cDCs may initiate and amplify acute lung inflammation of ALI by enhancing the Th1 immune response and ensuing cytokine production.

Objective To evaluate treatment effect of nodal lymphoma by using multiple b value diffusion-weighted MRI based on intravoxel incoherent motion (IVIM) model. Methods From November 2012 to November 2013, 22 patients with pathology confirmed lymphoma in Guangdong General Hospital were chosen. Patients were examined on a 1.5 T MR scanner with plain MRI scan and multiple b value diffusion-weighted MRI scan before and after cycle two of chemotherapy. According to chemotherapy response which evaluated after cycle 2 of chemotherapy, lymphoma nodes were divided into four groups:complete response (CR), partial response (PR), stable disease (SD), progressive disease (PD). Kruskal-Wallis H test was used to compare prechemotherapy D value, f value among CR, PR, SD groups. Nemenyi test was used to compare prechemotherapy D value between the two of CR, PR, SD groups. One-way ANOVA was used to compare D*value among CR, PR, SD groups. Paired-sample t test was used to compare D, f, D*value between before and after chemotherapy in PR group. CR and PR group were ascribed to curative group, and SD group ascribed to poor response group. ROC curve was used to evaluate the predictive efficiency of parameters derived from IVIM. Results Twenty-two lymphoma patients were scanned before chemotherapy and 21 patients were scanned after cycle 2 of chemotherapy. There were 49 lesions in CR group, 17 lesions in PR group, 8 lesions in SD group and no lesions in PD group. Prechemotherapy D value of CR, PR, SD group were (0.63±0.26)×10-3, (0.57±0.10)×10-3,(0.42±0.04)×10-3 mm2/s, respectively. There was significant difference among the three groups (H=12.944,P=0.002). There was no statistically difference of prechemotherapy D value between CR and PR group (χ2=0.072,P=0.965). Prechemotherapy D value was lower in SD group than that in CR group (χ2=12.090,P=0.002) and PR group (χ2=10.684,P=0.005). There was no statistically difference of prechemotherapy f value among CR, PR, SD groups (χ2=2.312,P=0.315) or D*value (F=0.535,P=0.588). D value significantly increased after chemotherapy in PR group [(1.03±0.37)× 10-3 vs.(0.63 ± 0.26)× 10-3 mm2/s, t=-4.781, P=0.001]. f value significantly increased after chemotherapy in PR group [(9.39 ± 4.52)% vs.(6.44 ± 3.25)%, t=2.294, P=0.036]. D* value slightly increased after chemotherapy but with no statistical difference in PR group [(99.72 ± 42.12)× 10-3 vs.(90.37 ± 45.33)× 10-3 mm2/s, t=-0.579, P=0.570]. When a D value of 0.48 × 10-3 mm2/s was used as the threshold value for predicting chemotherapy response, the best results were obtained with sensitivity of 100.00%and specificity of 75.76%.Conclusions Prechemotherapy D value can predict chemotherapy response and D value can monitor chemotherapy response in lymphoma.

Objective To conduct a pilot study on genome-wide in vivo protein-RNA interactions in E.coli.Methods Bacterial lysate was treated with RNase before the RNA fragments protected by proteins were extracted from treated lysate and used to construct cDNA library that was applied to high-throughput sequencing .Finally, the transcripts bound by proteins were obtained by bioinformatics analysis .Results A total of 3193 transcripts were obtained , including 2234 mRNAs, 47 sRNAs, 39 tRNAs, 11 rRNAs, and 862 intergenic regions .Conclusion Some information of transcripts interacting with proteins in E.coli is acquired , which will facilitate further studies of protein-RNA interactions .