Gene Expression Omnibus (GEO)can act as an international public resource platform storing and sharing experimental data generated from high-throughout research projects that utilize genome-wide expression strategies like microarray screening and analysis.Numerous data involving inflammatory carcinoma models is helpful for dissecting the regulatory network and molecular mechanism of oncogenesis,thus offering favorable clues for new thoughts in prevention and treatment of cancers.GEO originated from various disease models or processed experiments also provides researchers with powerful evidence for tracing functions of novel predictive genes.

Currently refractory and relapsed mantle cell lymphoma (MCL) continues to develope a poor prognosis, and the treatment remains a significant clinical challenge. The use of bortezomib in clinic provides a novel method for the treatment of refractory and relapsed MCL. This review summarizes the mechanism and efficiency of bortezomib in the treatment of MCL and the strategies for overcome of bortezomib resistance.

Primary lymphoma of bone (PLB) refers to non-Hodgkin lymphoma (NHL),which is an uncommon extranodal lymphoma,accounting for 1 % of all NHL,5 % of extranodal lymphoma and 7 % of primary bone tumor. Currently,the diagnostic criteria of PLB includes a primary focus in a single bone; positive histological diagnosis; and no evidence of distant soft tissue or distant lymph node involvement. The diversity,hereditery,and lower incidence of PLB make it difficult to diagnose. In order to decrease the rate of misdiagnosis and missed diagnosis,the clinical,pathological,and imaging characteristics of PLB in this study are analyzed.

AIM and METHOD To investigate the antibacterial activity (ED 50 value) of cefoperazonesodium and tazobactam sodium (4∶0 5) in vivo using minor modified Karbers method,which will offer some important evidences for clinical trial and therapy RESULTS The ED 50 value of cefoperazone sodium and tazobactam sodium(4∶0 5) and Cefoperazone sodium and sulbactam sodium (1∶1) in vivo protested against zymogenic staphylococcus aureus and Escherichia coli in mice infected by these bacteria were 92 0 mg?kg -1 and 3 4 mg?kg -1 ,164 0 mg?kg -1 and 6 9 mg?kg -1 ,respectively,and 256 5 mg?kg -1 and 38 9 mg?kg -1 in single composed compound of cefoperazone sodium,cefoperazone sodium and tazobactam sodium (4∶0 5) in vivo had more potent antibacterial activity compared with cefoperazone sodium and sulbactam sodium (1∶1) and single composed compound of cefoperazone sodium. The ED 50 value of cefoperazone sodium and tazobactam sodium (4∶0 5) protested against pseudomonas aeruginosa in mice infected was lower than that of Cefoperazone sodium and sulbactam sodium and single composed compound of cefoperazone sodium CONCLUSION Cefoperazone sodium and tazobactam sodium made in China has an markedly therapeutic effect on antibacterial activity protected against zymogenic staphylococcus aureus and Escherichia coli and pseudomonas aeruginosa in mice infected by these bacteria

Objective To investigate the effect of glycated albumin on recurrent cerebral infarction in elderly patients with diabetes mellitus. Methods All of 252 elderly acute cerebral infarction patients with diabetes mellitus were chosen from clinics. They were divided into 2 groups: A group ( glycated albumin <19.0% , 117 patients) and B group ( glycated albumin ≥ 19.0%, 135 patients). The clinical characteristics and the recurrent rate of cerebral infarction were compared between two groups. Results The levels of glycated albumin, fasting blood glucose, total cholesterol, low-density lipoprotein cholesterol and triglycerides in B group were significantly higher than those in A group:(21.00 ± 4.93)%vs. (16.75±1.72)%, (9.84 ± 2.89) mmol/L vs. (5.36 ± 1.00) mmol/L, (5.44 ± 1.30) mmol/L vs. (4.57 ± 1.00) mmol/L, (3.13±0.81) mmol/L vs. (2.58 ± 0.74) mmol/L, (2.34 ± 1.61) mmol/L vs. (1.74 ± 1.47) mmol/L, P<0.01 or<0.05. The recurrent rate of cerebral infarction in B group was significantly higher than that in A group: 53.3%(72/135) vs.36.7%(43/117), χ2 = 6.946, P = 0.008. Logistic regression analysis showed that the increase of glycated albumin was the independent risk factor of recurrent cerebral infarction in elderly patients with diabetes mellitus (P=0.048). Conclusions The recurrent rate of cerebral infarction is increased in elderly diabetes patients with high glycated albumin. The increase of glycated albumin is the independent risk factor of recurrent cerebral infarction.

Moyamoya disease is a rare cerebrovascular disease.Its incidence is higher in the East Asia.The pathogenesis of MMD remains unclear at present,but the epidemiological research both at home and abroad suggests that the genetic factors play an important role in the onset of MMD.This article briefly reviews the recent progress in research on MMD related genes.

Oceanic carbon monoxide(CO) has been of biogeochemical interest due to its significant role in global carbon cycle and the greenhouse effect. A headspace method coupled with ta3000 trace gas analyzer system for the determination of CO in seawater was developed. The effects of temperature, equilibrium time and water/gas volume ratio on the sensitivity of headspace analysis were studied in detail. The results showed that CO concentrations in seawater were measured successfully by the 50-mL glass-only syringes with a water/gas volume ratio of 44∶ 6 and an equilibrium time of 5 min at 20 ℃ room temperature. Under the optimized conditions, the linear range of concentrations of CO was 0-2.7×10~(-6), r=0.999,p<0.0001. The relative standard deviation of the analysis method was <4.4%, with a detection limit of 0.02 nmol/L. The average recovery of CO was 90.5%. The concentrations of CO in surface waters of the North Yellow Sea were measured using this method and ranged from 0.20 nmol/L to 3.13 nmol/L, indicating that this method can be successfully applied to the detection of the in situ CO concentrations in seawater.

Background:Myopathy due to smooth muscle cells( SMC)abnormalities is involved in the pathogenesis of diabetic gastroparesis(DGP),however,the relationship between myopathy and advanced glycation end products(AGEs)is not fully clarified. Aims:To investigate the ultrastructural changes of gastric SMC in patients with diabetes mellitus( DM)and the relationship between expressions of contractile proteins and AGEs. Methods:Full-thickness gastric specimens from 30 gastric neoplasm patients undergoing gastrectomy from July 2012 to December 2012 at the First Affiliated Hospital with Nanjing Medical University were collected. Of them 15 patients had DM( DM group)and the other 15 patients without ( control group). Ultrastructure of SMC was observed by transmission electron microscopy. Expressions of three contractile proteins[ myosin heavy chains( MHC),α-actin and calponin]and Nε-carboxymethyllysine( CML),the key component of AGEs in gastric muscular layer were determined by Western blotting,and expressions of MHC,α-actin and calponin mRNA were determined by real-time PCR. Correlations of mRNA expressions of three contractile proteins with protein expression of CML were analyzed by Pearson correlation coefficient. Results:In DM group,significant ultrastructural changes were found in gastric SMC,including disruption of gap junction,swelling of mitochondria,occurring of lipofuscin in cytoplasm,increase in cell membrane alveolae,and thickening of basal lamina. Protein and mRNA expressions of muscular MHC,α-actin and calponin were significantly lower in DM group than in control group(P all ﹤0. 01),while protein expression of CML was significantly higher in DM group(P ﹤0. 01). In gastric muscular layer of DM group, negative correlations were found between expressions of MHC,calponin mRNA and CML protein(r= -0. 59,P=0. 02;r= -0. 63,P=0. 01),but no correlation was seen between α-actin mRNA and CML protein(r= -0. 49,P=0. 06). Conclusions:Disruption of SMC ultrastructure,decrease in contractile proteins and increase in AGEs are existed in gastric muscular layer of DM patients,and there is a negative correlation between contractile proteins and AGEs. These changes may cause impaired contractility of SMC,and subsequently lead to gastric motility disorders in diabetic patients.

Objective To improve the efficacy of the Nd : YAG laser-induced chorioretinal venous anastomosis in the rabbits'eyes. Methods Immediately after branch retinal vein occlusion (BRVO) model was made by argon laser in 40 eyes of 20 normal rabbits, the rabbits were divided into two groups. In the first group (experimental group), there were 10 rabbits treated with the argon laser followed by the Nd : YAG laser induced chorioretinal venous anastomosis. In the second group (control group), the rabbits were treated only with the argon laser induced chorioretinal venous anastomosis. Results An anastomosis in experimental group was formed in 6 rabbits within 4 - 6 weeks after treatment while no anastomosis was formed in control group. During 2 - 6 months the hemorrhage of retina or vitrous with laser photocoagulation were absorbed within 8 weeks and no other severe complications were found. Conclusion Applying Nd : YAG laser can improve the efficacy of chorioretinal venous anastomosis in the rabbils eyes.

Fine needle aspiration cytopathology of the breast is an effective means of distinguishing malignant from benign,and the aspirated sample collections techniques is the primary and key part of fine needle aspiration.Fine needle aspiration cell block has been improved and used on preoperative breast cancer molecular portraits.This paper will provide an introduction to application of specimens collection techniques,application and development of fine needle aspiration cell block on preoperative breast cancer molecular portraits.