Twenty cases of infantile eczema were treated by combination of Tuina therapy and Western medications, and 18 cases were treated by Western medications. The therapeutic effective rate was respectively 100% and 90.0% in the two groups, with a difference between them (P＜ 0.01).

Chinese compound priscriptions of traditional herbs are widely used in the alternative therapy for all kinds of diseases It remains unclear how the compounds in different herbs interact each other and which exacting mechanism can complain their effects The components and pharmacological effects of the combination of Chinese herbs are not due to the simple mixture of single herb's The substance basis research of combination herbs is one of the important issue for understanding their pharmacological mechanism Here the recent progress on the substance basis research of combination Chinese traditional herbs is reviewed, including the combinative effects and quantity ratio relation study, herbs serum study, chemical compounds research, the methods and techniques in chemical components analysis, and application foreground of Chinese compound priscription

Objective To investigate the effects of wortmannin (WT) on leukemia cells cycle and BCL-2 protein expression. Methods 0 25?mol/L PI-3 kinase inhibitor wortmanin was used to treat K562 cells for 24 hours, and Arac was simultaneously used to induce the cell apoptosis. The specific fluoresent staining and FCM analysis were adopted to measure the cell cycle distribution and BCL-2 expression. Results Compared with the control cells, the proliferation index(PI) of the K562 cells treated by wortmannin was significantly lower, the cell number of G1 phase and the percentage of cell apoptosis increased, and the BCL-2 protein expression significantly decreased. Conclusion Wortmannin could inhibitedtheproliferationofK5 6 2cells ,andwascellcyclespecificagent (CCSA) .

Objective: To investigate the preventive and theraputic effects of Astragalus and Angelica mixture(A&A)on renal tubulointerstitial fibrosis after unilateral ureteral obstruction(UUO)in rats and their mechanisms. Methods: UUO rats were randomly divided into Sham, UUO, A&A or ACEI groups. A&A, ACEI or the same amount of water was administered by gavage beginning 24 hours before UUO preparation and continued through ten days after UUO. Sera and the kidney tissues were collected from each group on the tenth day. Scr and BUN were measured. Trichrome staining, measurement of tubulo interstitial damage index and immunohistochemical studies localizing ? smooth muscle actin(? SMA), TGF ? 1, fibronectin(FN), laminin(LN)were carried out. Results: In UUO rats, the tubular interstitial damage index, the expressions of ? SMA, TGF ? 1, FN and LN were all increased compared with those of Sham group. The tubulo interstitial damage index had positive correlation with expressions of ? SMA, TGF ? 1, FN and LN. A&A significantly ameliorated deterioration of renal function, tubulo interstitial damage index and inhibited the over expressions of ? SMA, TGF ? 1, FN and LN in UUO rats. These anti fibrotic effects were similar to those affected by ACEI. Conclusion: In renal interstitial fibrosis induced UUO rats, A&A retard the progression of renal fibrosis and renal function deterioration by inhibiting myofibroblasts and suppressing TGF ? 1 expression, which may consequently result in a decreased production of extracellular matrix.

To introduce the necessity,contents and means of vocational training among fresh nurses (who work in the first year after graduation),to explore how to enhance the comprehensive professional qualitiy training mode for graduated nurses.

Objective To explore the effect of swimming and caressing on neonatal jaundice and weight.Methods Two hundred and forty newborns were divided equally into control and observation group with random digits table.The former received care with caressing and the latter with swimming and caressing.The two groups were compared in terms of weights and jaundice indices at birth and 7d after birth.Results Seven days after birth,the jaundice index in the observation group were lower than that of the control group and the weights of the observation group were larger(P<0.05).Conclusion Swimming and caressing are helpful for the development of neonates by C-sect and the reduction of jaundice duration.

ObjectiveTo study the clinical and pathological characteristics of tubulointerstitial nephropathy associated with Chinese herb patent Guan Xin Su He Wan(GXSHW-TIN).Methods15 patients with GXSHW-TIN were studied.Clinical and pathological data were semi-quantitatively assessed.Relationships between medication and the incidence,clinical characteristics and the outcome of the disease were analyzed.ResultsAll the patients had chronic renal failure when GXSHW-TIN was diagnosed.They all got the disease after long-term taking of GXSHW in routine dosage.Most of the patients presented gastrointestinal dysfunction,abnormal urine analysis and mild to moderate anemia as onset symptoms.The pathological characteristics were similar to those of Guanmutong(Aristolochia manshuriensis Kom) induced chronic aristolochic acid nephropathy(AAN).ConclusionLong-term taking of GXSHW,which contains Radix Aristolochiae,might induce AAN.It indicates that GXSHW should be causious for clinical use,the ban of Radix Aristolochiae in the pharmaceutical market needs to be considered for prevention of AAN.

Aim To establish and evaluate Sprague-Dawley (SD) rats model with yang-deficiency and constipation at the direction of traditional Chinese medicine theories. Methods Rats model with yang-deficiency and constipation were induced by orally administration of white vinegar and active carbon-ice water. Changes of model rats in common biological characteristic, the number and time of excretion stool, colonic electromyography, gastrointestinal hormones and intestinal neurotransmitters were observed. Results Compared to the control group: changes of the model group in common biological characteristic including abdominal distension, puffed and withered hair, sluggish response, falling body weight, black and dry stool; Excretion time of the first granule stool became longer; Number of excretion stool within six hours became shorter; Intestine pushing rate decreased, and secretion of gastrointestinal hormones and intestinal neurotransmitters disordered, etc. After being treated with Tongbianling, the syndrome and indexes of model rats markedly improved. After 6 days′ resume, the model group in the experiment of excreting stool had markedly signification in comparison with the control group. Conclusion SD rats model with yang-deficiency and constipation could be stably established by administering white vinegar and active carbon-ice water.

Objective To explore the influence of cryopreservation by ladder-style freezing from low temperature refrigerator to liquid nitrogen on hemopoietic stem cell(HSC)transplantation.Methods From April 2001 to April 2006,31 cases treated with HSC transplantation were randomized to controlled-rate freezer-liquid nitrogen group(n=15)and refrigerator-liquid nitrogen group(n=16)in Department of Hematology of 1st People's Hospital of Yunnan Province.The hemopoietic reconstitution time was observed,and the viability of HSC was determined by trypan blue exclusion test in two groups.Results No significant differences were found in transfusion values of mononuclear cells(MNC)and CD34+ cells,rates of trypan blue exclusion and time of ANC0.05).Conclusion Cryopreservation of HSC can produce successful engraftment by ladder-style freezing which has cryoprotectant solution containing final concentrations of 3-percent hydroxyethyl starch,4-percent human serum albumin,and 5-percent DMSO,and its effect is the same as traditional controlled-rate method with 10-percent DMSO cryoprotectant.The new freezing procedure is faster and easier,and freezing cost is reduced.

Objective The increasing prevalence of infectious diseases is threatening the biological safety of donated blood in developing countries .This study was to analyze the prevalence of transfusion‐transmissible infectious related measures among first‐time ,voluntary blood donors from 1999 to 2013 in China .Methods From 1999 to 2013 ,all first‐time donors in the Xi′an Blood Service were screened for hepatitis B virus (HBV) ,hepatitis C virus (HCV) ,human immunodeficiency virus (HIV) and Trepone‐miapallidum (TP) and analyzed by trend test analysis .Results The positive rates of HBV ,HCV ,HIV ,and TP in the 415 657 blood donors were 1 .02% ,0 .55% ,0 .05% ,and 0 .46% ,respectively .The prevalence of HBV and HCV presented a decreased trend .Conclusion HBV infection is the primary threat to the blood safety ,while the increasing prevalence of TP and HIV might al‐so be a potential threat .