Objective:The separation effect for the enantiomers of ketoprofen on four diferent chiral columns was investigated and established a sensitive and stereospecific HPLC separation and determination method for ketoprofen enantiomers in animal plasma.Methods:The enantiomers of ketoprofen was resolved in the normal phase mode by using n-hexane∶isopropanol∶TFA(80∶20∶0.02)as mobile phase with UV detector at a wavelength of 254 nm.The flow rate was 1.0 ml/min.Results:Linear calibration curves were obtained in the range 0.1～10?g/ml for R-(+)-ketoprofen(r=0.999 8)and S-(+)-ketoprofen(r=0.999 6)in plasma.The detection limit(S/N=3) was 2 ng for each enantiomer.Conclusion:This method is simple,fast and can be used to detect optical purity of S-(+)-ketoprofen and monitor pharmacokinetic studies of ketoprofen enantiomers.

Congenital heart disease is a type of common birth defect and most can be cured by surgery with prominent prolongation of life expectancy.But,different kinds of arrhythmias accompanied by congenital heart disease,especially late onset postoperative arrhythmias have become one of the major causes of mortality in these patients.Management and effective treatment for fast and slow arrhythmias associated with congenital heart disease are quite critical.

Objective To investigate the effect and underlying mechanism of nocodazole on the inhibition of rVSMCs proliferation. Methods rVSMCs were divided into four groups, group A (normal culture), group B (serum-free culture for 24 h) , group C ( 18 h normal culture after 48 h of serum-free culture ) , and group D ( nocodazole treatment for 12 h after thymidine treatment for 12 h) . Flow cytometry, transmission electron microscopy, and metabolism measurements were performed and mitofusin-2 ( Mfn-2 ) expression was detected. Results Flow cytometry analysis showed rVSMCs of group B、C、D were arrested to G0/G1 , S and G2/M phases, respectively. Less and smaller mitochondria were observed in group D by transmission electron microscopy in nocodazole-treated rVSMCs. Compared with groups A and C, there were significant decreases in glucose and L-amino acid metabolism, levels of ATP, and marked increase in NADH in group D(P<0. 05). Western Blot showed that G2/M cell cycle arrest and nocodazole could induce up-regulation of Mfn-2 in rVSMCs(P<0. 05). Conclusion Nocodazole can block the energy metabolism and proliferation in rVSMCs, which is probably associated with the role of Mfn-2 on anti-atherosclerosis.

Objective To investigate the association between -45C→G mutation at promoter of human urate transporter 1 gene and primary hyperuricemia. Methods The allele frequency and genotypo distribution of -45 C→G mutation at promoter of human urate trans-porter 1 gene were determined by PCR-RFLP in 217 patients with primary hyperuricemia and 419 normal controls. Results The frequencies of the G allele and CG genotype at promoter of human urate transporter 1 gene in patients were significantly higher than that in normal controls (P = 0. 031, P = 0.031). The levels of serum uric acid (UA) and triglyceride (TG) in subjects of CG genotype were significantly higher than those in the objects of CC genotype(t=3.058, t=3.699, P=0.002, P<0.001). There were no significant difference in the levels of total cholesterol (TC), fasting plasma glucose (FPG), urea nitrogen (BUN), creatinine (Cr) between the two groups (P>0.05). Conclusion The -45 C→G mutation at promoter of human urate transporter 1 gene may be related to primary hyperuricemia.

Objective To investigate the effect of intraperitoneal injection of compound sophora injection in the treatment of advanced gastric cancer patients with malignant peritoneal effusion.Methods 60 advanced gastric cancer patients with peritoneal fluid were randomly divided into two groups according to the different treatment meth-ods.A group of compound sophora injection with intraperitoneal therapy(group A),treatment 2 times per week for four weeks,Another group of compound sophora injection with vein therapy (group B),for 4 consecutive weeks.The efficacy of the two groups was compared.Results The effective rate of group A was 58.1%,which was higher than 27.6% of group B,the difference was statistically significant(χ2 =5.69,P <0.05).Conclusion Intraperitoneal perfusion with compound sophora injection can improve the effect in the treatment of peritoneal effusion in patients with advanced gastric cancer,and the adverse reaction is mild,it is worthy of clinical application.

Twenty cases of infantile eczema were treated by combination of Tuina therapy and Western medications, and 18 cases were treated by Western medications. The therapeutic effective rate was respectively 100% and 90.0% in the two groups, with a difference between them (P＜ 0.01).

Objective To explore the interactions mechanism of job embeddedness of nurses with psychological capital,organizational commitment,intention to stay and their influencing factors.Methods By convenient sampling method,722 nurses were investigated with Job Embeddedness Questionnaire, Psychological Capital Questionnaire,Organizational Commitment Questionnaire and Nurses′Intention to Stay Questionnaire.Path analysis was made on relations between the variants.Results Night shift intervals pose positive influence on nurses′psychological capital,organizational commitment and intention to stay.Psychological capital can influence job embeddedness by both directly and indirectly,and can influence their intention to stay indirectly.Organizational commitment can influence job embeddedness strongly and directly (r = 0.494 ),and their intention to stay both directly and indirectly.Job embeddedness can influence their intention to stay directly (r=0.453 ).The model fitting is found desirable.Conclusions Nursing managers can enhance nurses′organizational commitment by developing the psychological capital to enhance nurses′organizational commitment effectively.Nurses with higher level of psychological capital and organization commitment will embed into their workplace more deeply, then increasing their intention to stay ultimately.

With the rapid development of internet and multimedia technology,the training of visual literacy has gained much more attention.In the teaching of Biochemistry,when confronting numerous visual tools in various ways for biological molecules research and rapid development of subject,teachers and students are required to have higher level of visual literacy.However,it is slighting and retarded to develop visual literacy education in China.Therefore,it is very important to emphasize the improvement of visual literacy in the teaching of Biochemistry,which can improve the ability in teaching design and learning method in this era of graphical interpretation.

Objective To make sure the proper time when using self-designed hemostatic belt to press vein after mainline. Methods Divide the subjects into 8 groups, using different pressing time to different group, and then observe the condition of petechia. Results There are significant differences in the different groups about the condition of petechia, P

Increasing evidence has proved that phenotypic changes occured in residential renal cells during the process of renal diseases. The phenotypic change of mesangial cell plays a key role in the development of glomerular sclerosis, while the phenotypic changes of tubulo-interstitial cells have close relationship with the progress of interstitial fibrosis. Understandings of these phenotypic changes and their regulations may help us know better of the mechanism of renal diseases and find out ways to block the disease development.