Objective: To verify the feasibility and reliability of the pilot process of irbesartan and hydrochlorothiazide tablets. Methods:Three batches of the tablets were produced according to the determined formula and process, and the key parameters in the pilot production were verified. Results:All the indices for each batch of irbesartan and hydrochlorothiazide tablets conformed to the re-quirements of the quality standard. Conclusion: The pilot process of irbesartan and hydrochlorothiazide tablets is feasible, and the technological process and parameters can ensure the product quality with promising reliability and stability.

Objective To evaluate the clinical and biochemical characteristics of type 2 diabetics with hyperuricemia and to investi-gate the association between serum uric acid and the number of metabolic syndrome components. Method In the cross-sectional study of 547 type 2 diabetics, we recorded body mass index (BMI), systolic blood pressure(SBP), diastolic blood pressure (DBP), and collected blood samples to measure serum uric acid (UA), fasting plasma glucose (FPG), glycosylated hemoglobin (HbAlc), creatinine (Cr) and blood lipid profile, including tfiglyceride (TG), total cholesterol (TC), low-density cholesterol LDL-C) and high-density cholesterol (HDL-C). Results Compared with the normal UA group, the diabetic patients in the high UA group had significantly increased levels of BMI, SBP, DBP, FPG, Cr, TG, TC and HbA1c (P <0.05, P <0.01, P <0.05, P <0.01, P <0.01), and decreased level of HDL-C(P < 0.05). There was no significant difference in the level of LDL-C between the two groups (P > 0.05). According to their possession of 1, 2, 3 or 4 components of metabolic syndrome, 547 cases were divided into four groups (MSI, MS2,MS3 and MS4). There were significant difference in the levels of UA, BMI, SBP, DBP, Cr, TG, TC, LDL-C and HDL-C among MSI, MS2, MS3 and MS4 (All P < 0.05). Multiple regression analysis showed that UA concentration was positively correlated with sex, TG and Cr (Beta:0. 088,0. 350, 0. 124; P < 0.05, P <0.01, P <0.01) and negatively correlated with HDL-C and HbA1 c(Beta: -0.107, -0.124 ; P <0.01, P <0.01). The con-centration of UA was strongly correlated with serum TG. Conclusions Serum UA level in type 2 diabetics was significantly elevated as the number of metabolic components increased. Abnormal TG had great influence on serum UA. Hyperuricemia was considered to be a dangerous factor of the metabolic syndrome.

Objective To study the correlation between blood lactic acid level and PCIS scroing system.To study the relationship between dynamic lactic acid monitoring indexes and prognosis of critical patients.Methods Differences of mortality,incidence of MODS and shock,PCIS score between two groups were compared.The patients were divided into 3 groups by PCIS score to compare pathogenetic conditions prognosis, then to study the were divided into death group and survival group by the hospital prognosis. Differences of their lactic indexes and other clinical indicators which reflected organ/system status were compared, prognostic-significant lactic indexes were selected by mu fti-variable logistic analysis. Results Patients with elevated lactic acid level had more incidence of shock and MODS, higher PCIS scores and higher mortality, the differences had statistical significance compared with the normal lactic acid group. There were significant linear correlation between ICU lactic acid level, peak lactic acid level and PCIS scores respectively. For the patients with elevated lactic acid level, peak lactic acid level, 12h lactic clearance had significant correlation with prognosis. Conclusion PCIS scoring system had a significant linear correlation with lactic acid level. For the patients with elevated lactic acid level,peak lactic acid level,12h lactic clearance were good indicators to evaluate patients' prognosis.

Objective To investigate the factors that influence the patients to understand the stroke they suffering from.Methods 61 stroke patients were investigated with a questionnaire designed by ourselves.ResultsThe age,sex and educated level affected the stroke patients' understanding,but the time after onset,the degree of locomotory injured and hemiplegic side did not.ConclusionDistinguishing direction of rehabilitation should be established according to the age,sex and educated level of patients.

Objective To investigate the changes in molecule levels of Ras-ERK signal pathway of K562 cells treated with simvastatin in vitro,and to illustrate that simvastatin inducing the changes in molecule levels of Ras- ERK signal pathway is involved in regulation of proliferation and apoptosis of K562 cells. Methods K562 cells,the chronic myelocytic leukemia(CML) cell lines,were cultured and treated with simvastatin in vitro and proliferation activity of K562 cells was detected by MTT. The changes of apoptosis rate and cell cycle of K562 cells were measured by flow cytometry(FCM). The molecular changes of Ras-ERK signal pathway were analyzed by RT- PCR in transcriptional level. Results The proliferation of K562 cells was inhibited by simvastatin,and G_0-G_1 arrested in K562 cells and significant apoptosis rate was observed with FCM. Most molecules of Ras- ERK signal pathway expressed differentially at transctiptional level. Conclusion Simvastatin probablely inhibit proliferation and induces apoptosis of K562 cells,depending on Ras-ERK signal pathway which is involved in cell proliferation and apoptosis.

Objective To investigate the clinical effect and safety of laparoscopic-assisted vaginal radical hysterectomy (LAVRH) and pelvic lymphadenectomy in early-stage cervical cancer.Methods The clinical data of 126 patients with cervical cancer were retrospectively analyzed.63 cases received LAVRH surgery were considered as study group,and 63 cases received laparotomy were selected as control group.The operation time,intraoperative blood loss,intestinal function recovery time,the average hospitalization days,surgical complications,recurrence incidence,etc were compared between the two groups.Results The operation time,intraoperative blood loss,intestinal function recovery time,the average hospitalization days had significantly statistical differences between two groups(P ＜0.05).The surgical complications and recurrence rate had no significantly statistical differences between two groups (P ＞0.05).Conclusion LAVRH and pelvic lymphadenectomy has the advantages of minimally invasive,less blood loss,faster recovery,etc.It is an effective method for the treatment of early-stage cervical cancer.

Objective To explore the application of scene type trace method in physiology teach-ing reform. Methods 2009 grade clinical medicine students in Zunyi Medical College were randomly di-vided into two groups:control group (n=111) and experiment group (n=108). Students in control group were taught by traditional teaching method while those in experimented group by scene type trace method. According to the questionnaire survey and the final exam statistical analysis,the effects of teach-ing were evaluated. SPSS 15.0 software was used to do statistical analysis. Qualitative data were analyzed by chi-square test of completely randomized design and two sets of frequency distribution while quantita-tive data by independent samples t test. P<0.05 means that difference is statistically significant. Results There were statistically differences in satisfaction degree of two teaching methods. More students were sat-isfied with scene type trace method than traditional teaching method. Final exam achievement in scene type trace method group(79.90±14.25,90.74%)were higher than that in the traditional teaching method group(72.35±17.09,83.78%). Conclusions Physiology teaching scene type trace method is helpful to improve the students' learning initiative and significantly improve students' learning achieve-ment.

Gossypol has been shown to cause a side effect hypokalemia. This study was designed to evalulated the possible role of gossypol in relation to renal potassium loss by using histochemical method for comparative analysis of gossypol and gentamicin, a well known tubulo-toxic agent, as positive control with respect to nephrotoxic demage in rats and guinea pigs. The results indicated that gossypol had obvious effect on reabsorption function by inducing transitional decrease of membrane enzymes activities of renal tubular cells but had no effect as comparable with gentamicin to cause structureal damage of tubular cells.

Objective To investigate angiographic manifestations and interventional treatment of renal arteriovenous fistulas. Methods Renal arterial angiographies were performed in 20 patients from multiple hospitals.These patients suffered from repeatedly intermittent hematuria,but ultrasound,KUB,IVU,CT and endoscopy could not contribute to the diagnosis.The angiographic manifestations and treating procedure were analyzed. Results All the 20 cases were diagnosed as renal arteriovenous fistula by means of angiography.According to the image manifestations,these cases were classified into three types:(1)deformed branches and shunt in 11 cases;(2)high discharge shunt without deformed banches in 4;(3)concealed shunt showed by superselective segmental artery angiography but negative by renal artery angiography in 5.All fistulas of the 20 cases were successfully embolized with different materials including gelfoam,sodium morrhuate,ethanol,PVA,TH-glue,coils,etc. Conclusions Superselective renal angiography and embolization are the most important and effective tools for diagnosis and treatment of renal arteriovenous fistula.

Objective To investigate the effect of the dihydroartemisinin (DHA) on the human low-differentiated lung adenocarcinoma cell line A549 and to explore its mechanism. Methods The A549 cells at logarithmic growth phase were divided into control group and DHA group. The cells in the control group were incubated with conventional reagent, and the cells in DHA group were incubated with 500 nmol/L of DHA. After incubation for 72 hours, methyl thiazolyl tetrazolium (MTT) assay was used to examine the proliferation of A549 cells in the two groups. Gene expression of p85, Akt, Bax and Bcl-2 was detected by real-time fluorescence quantitative polymerase chain reaction (PCR) . The protein expression of p85, Akt, p-p85, p-Akt, Bax and Bcl-2 was detected by Western blotting method. The activity of Caspase3 was measured by Caspase3 colorimetric assay kit. Results Compared with the control group, the proliferation rate of A549 cells in DHA group was significantly decreased ( P<0.01) . The RT-PCR results showed that the mRNA expression levels of p85 and AKT had no obvious difference between the two groups, but the mRNA expressien level of Bax was increased ( P<0.001), and the mRNA expressien level of Bcl-2 was decreased ( P<0.05) . The Western blotting results showed that there was no significant changes of p85 and Akt proteins between the two groups (P>0.05), but p-p85, p-Akt and Bcl-2 protein expression levels were significantly decreased ( P<0.01) , and Bax protein expression was increased ( P<0.01). Moreover, the activity of Caspase3 was also enhanced ( P<0.001). Conclusion DHA can reduce the proliferation of A549 cells and increase the apoptosis of A549 cells, and its mechanism probably has relationship with the inhibition of PI3K/Akt signaling pathway.