Objective To investigate the effect and underlying mechanism of nocodazole on the inhibition of rVSMCs proliferation. Methods rVSMCs were divided into four groups, group A (normal culture), group B (serum-free culture for 24 h) , group C ( 18 h normal culture after 48 h of serum-free culture ) , and group D ( nocodazole treatment for 12 h after thymidine treatment for 12 h) . Flow cytometry, transmission electron microscopy, and metabolism measurements were performed and mitofusin-2 ( Mfn-2 ) expression was detected. Results Flow cytometry analysis showed rVSMCs of group B、C、D were arrested to G0/G1 , S and G2/M phases, respectively. Less and smaller mitochondria were observed in group D by transmission electron microscopy in nocodazole-treated rVSMCs. Compared with groups A and C, there were significant decreases in glucose and L-amino acid metabolism, levels of ATP, and marked increase in NADH in group D(P<0. 05). Western Blot showed that G2/M cell cycle arrest and nocodazole could induce up-regulation of Mfn-2 in rVSMCs(P<0. 05). Conclusion Nocodazole can block the energy metabolism and proliferation in rVSMCs, which is probably associated with the role of Mfn-2 on anti-atherosclerosis.

Objective To explore the value of a new electrodiagnostic approach for evaluating motor nerve root function in patients with S1 radiculopathy. Methods Thirty healthy subjects and 30 patients with clinical mani-festations of unilateral S1 radiculopathy were recruited. Bilateral compound muscle action potentials evoked by magnetic stimulation of the first sacral nerve root were recorded from the soleus of all the subjects. F wave and M responses to electrical stimulation of the bilateral tibial nerves at the popliteal fossa were also recorded. The peripheral motor conduction time (PMCT) and the motor root conduction time (MRCT) were calculated and compared between the two groups. In addition, needle electromyographic examination (NEE) was performed on the affected side to detect any possible EMG abnormalities. Results The norm established with the normal subjects was 3.45±0.39 ms for the MRCT, and 0.28±0.15 ms for the inter-side difference in the MRCT. In the 30 patients, the mean MRCT and PMCT values on the affected side were prolonged. Of the 23 patients who received NEE, 6 had EMG abnormalities. The agreement between the NEE and MRCT diagnoses was 82.6%. Conclusion MRCT can be used reliably for non-invasive estimation of motor nerve root function and to help diagnose the S1 radiculopathy.

Objective To explore a new electrodiagnostic approach using the H reflex elicited by magnetic stimulation of the S1 nerve root and F waves to evaluate sensory nerve root function in patients with S1 radiculopathy.Methods Thirty normal subjects and 30 patients with unilateral S1 radiculopathy were recruited in this study.H reflex and M response were recorded from the bilateral soleus of all the subjects by magnetic stimulation of S1 nerve roots.F and M wave responses elicited by electrical stimulation of bilateral tibial nerves at the popliteal fossa were also recorded.The sensory root conduction time (SRCT) was calculated.Correlations of age and body height with SRCT in the healthy subjects,and between SRCT and pain in the patients with S1 radiculopathy were analyzed.Results The mean values of normal subjects were 3.10 ± 0.44 ms for SRCT,and 0.13 ± 0.19 ms for inter-side SRCT differences.In the 30 patients with S1 radiculopathy,H reflex could not be elicited from 4 patients.Among the remaining patients,the SRCT of the affected side was prolonged significantly (3.90 ±0.65 ms),and the mean value of the inter-side difference increased significantly (0.90 ±0.50 ms).A regression equation correlating SRCT with height was developed,but no significant correlation between SRCT and age in the normal subjects was revealed.There was positive correlation between SRCT and the severity of pain among the patients.Conclusion SRCT can be used as a new electrodiagnostic index in estimating sensory nerve root function in patients with S1 radiculopathy.

Objective To analyse the effect of progesterone on peripheral blood corticotropin releasing hormone ( CRH ) and delivery time in women with premature rupture of membranes ( PROM ) .Methods 80 patients who were diagnosed with PROM in Department of Obstetrics and Gynecology, China Medical University were collected.Randomly divided into dexamethasone (DEX) group, dexamethasone plus progesterone (DEX+P) group, progesterone ( P) group and control group, three groups were detected on admission, admission 24 h, 48 h on peripheral white blood cell count, C-reaction protein, CRH level and time of delivery, neonatal weight, and analysis of CRH the level of the correlation and delivery time.Results Compared with the other three groups, the level of CRH in peripheral blood of DEX group were higher (P<0.05);CRH (P<0.05) increased faster;shorter delivery time (P<0.05); the level of CRH was negatively correlated with delivery time (r=-0.832, P<0.05).The results were statistically significant.Conclusion Dexamethasone treatment can make the premature rupture of fetal membranes of peripheral blood CRH levels rise, shorten the delivery time, progesterone can inhibit this process.

Objective To study inhibitory activity of anthraquinone in Polygonum Cillinerve(Nakai) Ohwi (anthraquinone in PCO) to nitrosation. Methods Anthraquinone was extracted from Polygonum Cillinerve(Nakai) Ohwi with sulfuric acid and chloroform as solvents. The capability of scavenging sodium nitrite and disconnecting nitrosamine synthesis with anthraquinone in PCO were determined by spectrophotometry under simulated human gastric juice. Results Both the sodium nitrite scavenging rate and the nitrosamine synthesis disconnecting rate showed the positive correlation with the concentration of anthraquinone in PCO. The strongest capability of scavenging sodium nitrite was 53.5%, and the strongest capability of disconnecting nitrosamine synthesis was 71.3%. Conclusion Anthraquinone in PCO had strong capability of scavenging sodium nitrite and disconnecting nitrosamine synthesis.

During percutaneous coronary intervention,thrombotic storm which is mediated by hypercoagulable state,mechanical distension induced-plaque rupture,platelet activation and adhesion is still the main cause of cardiovascular adverse events.The mortality rate is extremely high if not treated properly.Thrombotic storm can be diagnosed quickly through coronary artery angiography and myocardial blush grades.Once coronary thrombosis occurs,medicine including platelet Ⅱ b/Ⅲ a receptor antagonist tirofiban or vasodilators can rapidly improve coronary flow and effectively treat it.

Chronic obstructive pulmonary disease (COPD) is the leading death cause of pulmonary diseases, ranking the fourth of global death causes. Self-management program is able to gradually improve the Airways of COPD patients with ventilation, reduce the degree of lung failure, elevate the health status as well as promoting self-efficacy, which is the key process to effectively control COPD relapse. In recent years, more and more domestic and foreign academic employ the widely-used network technology and carry out a large number of information study on COPD self-management intervention. This article reviewed literatures about the intervention methods on self-management of patients suffered from COPD, aimed at providing a better basis for exploring more effective self-management.

The role of lipid-regulating capsule in the regulation of experimental dislipidemia was studied in SD rats. The SD rats were divided into 6 groups: group A (normal control)，group B to F (experimental hyperlipidemia). The rats in the groups C，D and E received the capsule in a daily dose of 2.5 g，5 g and 10 g/kg respectively for 3 weeks，while the rats in the group F received Simvastatin in a daily dose of 7.5 mg/kg for 3 weeks. By comparison with the group B after 3 weeks，the levels of plasma total cholesterol (TC) and low density lipoprotein (LDL-C) were very significantly decreased in the groups C，D，E and F(P<0.01)，the value of plasma total triglyceride (TG) was very significantly decreased in the groups D，E and F (<0.01)，and the level of high density lipoprotein was significantly increased in the groups C，D and E (P<0.05)，but very significantly increased in the group F (P<0.01). It was suggested that the therapeutic efficiency of the lipid-regulating capsule in low，middle and high dosage was the same as that in high dose of simvastatin for the SD rats with high plasma TC and LDL-C，and middle and high doses of the capsule had the same effect of simvastatin on the plasma TG.

OBJECTIVE:To establish the Microbial limit test(MLT) for Danggui funing drop pills.METHODS:The recovery rates of 5 tested strains treated by Danggui funing drop pills were detected and the MLT method for the control bacteria was validated.RESULTS: Danggui funing drop pills exhibited strong inhibitory effect on staphylococcus aureus and bacillus subtilis,but showed no effect on escherichia coli,candida albicans and aspergillus niger.CONCLUSION: By membrane-filter procedure,the antibacterial effect of Danggui funing drop pills can be eliminated and the bacterial count can be conducted;however,by routine method,the test of control bacteria is feasible.

0.05),while there was significant difference among other groups(P