OBJECTIVE:To discuss the effect of recombinant human growth hormone(HGH)on serum sndotoxin,inflamma-tory cytokines,wound healing time,hospitalization time and prognosis of elderly patients with severe burn. METHODS:96 elder-ly patients with severe burn in our hospital were randomly divided into treatment group (48 cases) and control group (48 cases). All patients received wound surface care,nutritional support,maintaining water and electrolyte and acid-base balance. Control group received 1 ml 0.9% Sodium chloride solution,sc,qd. Treatment group received 0.3 IU/kg. Recombinant HGH for injection, sc,qd. All patients were treated for 21 d. Serum endotoxin content in 2 groups before and 14,21 d after treatment was compared, inflammatory cytokines (TNF-α,IL-6, IL-8) before and after treatment,wound healing time,hospitalization time and the inci-dence of multiple organ disorder syndrome(MODS),death and systemic infection were observed. RESULTS:There was no signifi-cant difference in serum endotoxin content before and 14 d after treatment(P>0.05);after 21 d,serum endotoxin contents in 2 group obviously decreased,and treatment group was significantly lower than control group,with statistical significance(P<0.05). After treatment,IL-8 level in treatment group was significantly higher than control group,TNF-α and IL-6 were significantly lower than control group,with statistical significances (P<0.05). Wound healing time and hospitalization time in treatment group were significantly shorten than control group,with statistical significances(P<0.05). The incidence of MODS,death and systemic infec-tion in treatment group were significantly lower than control group,with statistical significances(P<0.05). CONCLUSIONS:The application of recombinant HGH in elderly patients with severe burn can reduce toxins absorption of patients and decrease inflamma-tory cytokines to promote wound healing and improve prognosis.

Objective To assess the reliability and validity of standardized facial photographs under natural head position (NHP) in assessment of mandibular asymmetry cases. Methods Standardized facial photographs (SFP) and posteroanterior cephalometry (PAC) were taken under natural head position from 25 mandibular asymmetry eases (14 boys and 11 girls), aged 14 to 29 years old. Distances from 8 landmarks to the vertical reference line were measured. The difference and the asymmetry ratio of two sides were calculated respectively. The relationship between the measurements of SFP and PAC were compared, and the repeatability of landmarks was assessed. Results Six measurements were significant different between two sides, 3 from SFP and 3 from PAC. Measurements from two ways were correlated a cer-tain extent. The repeatability of landmarks was satisfied. Conclusion Measurements from standardized facial photographs under NHP pres-ent a clinical useful method to quantify mandibular asymmetry.

Helicobacter pylori(Hp)infection is the cause of a variety of gastrointestinal disease. Currently,there are many therapeutic regimens for Hp eradication,however,because of the increase of Hp resistance and adverse effects in the treatment,the eradication rate of Hp is not very satisfactory. Studies showed that probiotics as an adjuvant may improve the eradication rate and reduce the incidence of adverse effects in eradication therapy for Hp. This article reviewed the advances in study on application of probiotics in eradication of Hp.

ve To explore a method for determination of magnesium in drinking water by air-acetylene flame atomic emission spectrometry. Methods The content of magnesium in drinking water was directly determined by air-acetylene flame atomic emission spectrometry using hydrochloric acid as media and lanthanum solution as masking agent. Results The concentrations of magnesium in drinking water were possitively correlated with the intensities of flame atomic emission spectrum in the range of 5~200?g/ml with a regression equation of A = 0.000 87C+0.0019(r=0.992 2). The detection limit was 5.0?g/ml. The recovery rates of a standard material added-water samples were 101.8% ~ 107.5% . The relative standard deviation was not more than 3.3% . The com-monly coexisting ions with a certain concentrations, such as K, Na, Ca, Zn, Fe, Mn, Cd,Pb and Cu, didn't interfere the determination of magnesium in water sample. Conclusion This method could be applied to determination of magne-sium in drinking water.

It has been a sub - rale in hospital that many doctors and hospitals themselves try every means to get maxinoum interests from patients in many places. Througyh the " Hu Wei Min" case, this essay analyzed the reasons and drawbacks of asking extra money from patients and discussed related social ethical problems and therefore brings out a counter - plans to solue the problem.

Interventional treatment provides another option for patients with ischemic cerebral vascular disease. It has been accepted by doctors and patients for its miniinvasive and good effect. The doctors performing the operations scatter in different departments, therefore they have different special professional opinions about the diagnosis and treatment, outcoming with deviation form the proper treatment indications. Revising treatment notion with special emphasis on the brain function improvement, standardized operation and strict discipline are urgently needed for the promising management and development with combination of the new comprehensive knowledge and concepts, especially follow the time indications.

Objective To discuss the value of mammotome biopsy of breast cakified lesions under the real time guidance of mammography.Methods The data of 42 patients with 44 mammary gland calcification(2 patients with bilateral lesions)who underwent mammotome biopsy under the real time guidance of a mammography from Jan.2011 to Feb.2012 in the First Affiliated Hospital of Chongqing Medical University were retrospective analyzed.Rapid paraffin pathology was conducted.Results Among the 44 lesions,34 were benign(27 hyperplasia,2 fibroadenoma,1 lipoma,1 cyst,3 others)and 10 were breast cancer(a benign lesion diagnosed as breast cancer by postoperative biopsy was included).Among the 42 patients,32 patients had benign lesions,accounting for 76.2％ (32/42),10 patients had breast cancer,accounting for 23.8％ (10/42).All lesions were completely resected during the operation.Malignant lesions were given treatment such as modified radical mastectomy,breast conservation surgergery,chemotherapy and so on.A single lesion resection took about 20 minutes.During the follow-up of 3 months,no significant event happened.Conclusions Through the qualitative diagnosis of mammotome biopsy under the real time guidance of mammography,we can get early diagnosis of breast cancer.Early diagnosis and early treatment can improve the life quality and survival time.It is more important to detect early breast cancer which was considered benign lesions clinically.Mammotome biopsy under the real time guidance of mammography has several advantages including easy to operate,accurate positioning and less complications,making it the preferred approach in managing mammary gland calcification especially suspicious malignant calcification.

Human epidermal growth factor receptor 2 (HER-2/neu) is an important prognostic predictor and the key predictor of anti-HER-2 therapy of breast cancer. Accurate testing of HER-2 status for breast cancer patients is important in clinical practice. As of this writing, the American Society of Clinical Oncology and the College of American Pathologists recommend three methods for HER-2 detection, namely, immunohistochemistry, fluorescence in situ hybridization, and bright-field in situ hybridization. The abovementioned methods have their own advantages and disadvantages. New methods, such as multiplex ligation-dependent probe amplification, quantitative reverse transcription polymerase chain reaction, and RNA in situ hybridization, are currently applied to detect HER-2 status. New technologies not only make up for the shortcomings of routine methods but also have unique benefits that can meet the demands for HER-2 testing of some breast cancer patients. Thus, these methods are promising for clinical applications and can improve clinical diagnosis and treatment. The characteristics, advantages, and drawbacks of these technologies are introduced and reviewed in this paper.

At present,there is no unified standards of treatment in elderly advanced non-small cell lung cancer (NSCLC),but to elderly patients with higher KPS scores,radiotherapy or chemotherapy alone has gradually become the main treatment measures.The concurrent chemoradiotherapy can benefit to part of the elderly patients,and it is worth further clinical research.Targeted therapy has exactly effect,and the adverse effect is mild,which provides a new treatment model for elderly advanced NSCLC patients.However,the hospital costs are relatively high,which can not be accepted by ordinary family.So targeted drugs and the model of combination treatment can not be applied extensively.

Objective To investigate the reliability of cast measurements by comparing Photoshop and conventional hand-held method. Methods Maxillary standard plaster models from 10 orthodontic cases with crowding were enrolled in this study. The space available and the space required of anterior region were measured by using Photoshop in the digital pictures or conventional method used by 5 observers respectively.The measurement results of the same sample among every observer were analyzed with ANOVA, and the difference between the 2 methods was calculated and paired t test was used to analyze the data. Results The repeatability of measurement results was not good when the space available was measured with conven-tional method by different observer( M1 ～ M5 ), and the measurement results of M1 were significantly different from that of M3 and M5 respectively( P ＜ 0.05 ). The repeatability of measurement results was relatively good when the space available was measured by using Photoshop, and the measurement results of M5 were significantly different from other observers ( P ＜ 0.05 ). The measurement results of space required measured by different observer were very close, and the reproducibility was excellent when they were measured by means of Photoshop or conventional methods. Conclusion There were no much difference between Photoshop and conventional method to measure the required space. Photoshop had significant advantage over conventional method when available sRace was measured.