Objective:To study relativity between tumor metastasis and the level of immune competent cells from patients with malignant carcinoma.Methods:The percentage of NK cells and CTL cells and monocyte/macrophage cells were measured and analyzed in tumor tissue and peripheral blood from patients with malignant carcinoma by flow cytometry.Results:NK cells, CTL cells and monocyte/macrophage cells of tumor tissue and peripheral blood were lower than those of normal control groups ( P

Objective To investigate the effect of radiotherapy on cellular kinetics of peripheral blood monocytes in patients with malignant tumors. Methods In patients with malignant tumors, apoptosis and proliferative activity of peripheral blood monocytes were monitored and compared before, during and after radiotherapy. Results Apoptosis and S phase fraction in cancer patients on radiotherapy were higher as compared to these without radiotherapy or the normal control group (8.93%?6.94%, 0.20%?0.20%, 0.15% ?0.13%, and 1.23%?1.15%, 0.54%?0.46%, and 0.33%?0.26%,respectively,F=5.77, P

Objective:To study the influence of surgical operation on cellular kinetics in peripheral blood monocytes of patients with malignant tumors.Methods:Apoptosis level and proliferative activity were measured and compared between before and after surgical opration in peripheral blood monocytes of patients with malignant tumors.Rusults:Apoptosis level of patients with malignant tumors after surgical operation was lower than that of patients before surgical operation( P

Objective: To study tumor chemotherapy on IL-2R express in peripheral blood cells of patients with malignant tumors.Methods: CD + 25 cells percentage rate of peripheral blood cells in patients with or without chemotherapy was done.Results: CD + 25 cells percentage rate in malignant tumor was lower than those of normal contrlos (P

Objective To comparatively study the difference status of lymphocyte subsets and activated T lymphocyte between preoperation and intraoperation,and to investigate the influence of operation on lymphocyte subsets and their activation in the pa-tients with malignant tumors.Methods The lymphocyte subsets and their activated cells were determined in 42 cases of common malignant tumors by the flow cytometry.Results The levels of CD3 + ,CD4 + ,CD8 + cells during intraoperation were obviously de-creased compared with preoperation,the difference in CD3 + and CD4 + had statistical significance(P <0.05),while the difference of CD8 + had no statistical significance(P >0.05).NK,CD19 + ,CD3 + HLA-DR+ were significantly increased,the difference of NK and CD19+ had no statistical significance(P >0.05),while the difference with CD3 + HLA-DR+ had statistical significance(P <0.05). Conclusion The cellular immune function in the patients with malignant tumor is in inhibitory status.Operation makes the cellular immune function to be further injured.The stress responses of operation and trauma increase the expression of NK,CD19 and acti-vated T lymphocytes.It is suggested that the patients with malignant tumor should use the immune response modifier before opera-tion to enhance the cellular immune function for ensuring the effect of postoperative radiochemotherapy sequential treatment.

Objective To improve the sensitivity of detection of protein P16 by FCM.Methods Protein P16 of blood cells and tumor cells in biopsy samples were labeled simultaneously with anti-CD45 and anti-P16 fluorescent antibody.The rates of CD45+P16+ cells and CD45-P16+ cells were calculated by FCM.Results The low expression rates of protein P16 in normal blood cells and in tumor cells in biopsy samples were 15.39%(6/39) and 69.23%(27/39),respectively, which had a significant differences (P

Objective:To investigate the clinical features and feasibility genetic diagnosis in a hereditary hemorrhagic telangiectasia (HHT) family,and to explore the application of gene mutation testing in HHT diagnosis.Methods:Medical histories and clinical features of a family were analyzed to diagnose HHT patients and suspected individuals according to the clinical diagnostic criteria.Sequence analysis of endoglin (ENG) and activin A receptor like type 1 (ACVRL1) gene in the proband was performed with PCR and Sanger sequencing technology.After the possible pathogenic mutation was identified in the proband,the specific mutation was detected in the suspected individuals and part of other family members.Then the genetic diagnoses were concluded.Results:There were 5 family members in 4 generations manifested with epistaxis.According to the clinical diagnosis criteria,the proband with epistaxis,mucocutaneous telangiectases,visceral arteriovenous malformation and family history was diagnosed as HHT;while 2 survival family members with epistaxis and family history were suspected individuals.A substitution mutation in the 5'-untranslated region(5'-UTR) of ENG c.1-127 C＞T was detected in the proband and the 2 suspected individuals,which did not exist in other family members.Based on the clinical and genetic findings,the 2 clinically suspected individuals were diagnosed as HHT.Conclusion:There is great variability of the clinical manifestations among HHT patients.ENG c.1127 C＞T mutation is the possible pathogenic variant of the HHT family.A combination of clinical and genetic diagnosis could improve the diagnosis and treatment of HHT.