Objective To investigate the clinical effect of enzyme linked immunosorbent assay (ELISA) in detecting hepatitis C antibody. Methods 200 cases of patients with hepatitis C was measured by ELISA, the gold standard method for detection of hepatitis C virus antibody, recording the test results, the data input SPSS software to give the corresponding analysis and conclusions. Results 200 cases of patients with hepatitis C were successfully completed the gold standard method and ELISA method for detection of hepatitis C virus antibody, the analysis showed that positive rate of ELISA method for detection of hepatitis C virus antibody was significantly higher than that of the gold standard method, the positive rate of ELISA was 82.50％, the positive rate of the gold standard method for was 50.00％, results suggested that HCV detection rate of ELISA method is significantly better (P<0.05). Conclusion The method of ELISA can be used for the detection of hepatitis C antibody, which has positive significance in improving the diagnostic accuracy of hepatitis C, and is beneficial to protect the efficacy and quality of life of patients.

Objective To observe the effect of continuity self -management education on the emotional state and quality of life in patients with peritoneal dialysis and provide guidance for health intervention.Methods 236 patients with peritoneal dialysis were selected and randomly divided into two groups.The control group(118 cases)was just given routine nursing care,the continuity self -management education group(118 cases)was given continuity self -management education and routine nursing care.After 4 months,the differences of anxiety,depression and quality of life between the two groups were evaluated.Results Four months after the intervention,anxiety and depression scores of the two groups became lower,which of the continuity self -management education group decreased more significantly than the control group(anxiety:t =9.860,P =0.000;depression:t =15.265,P =0.000).The quality of life scores of the two groups became higher,which of the continuity self -management education group were better than the control group (somatization:t =26.767,P =0.000;force:t =8.090,P =0.000;sensitive interpersonal relationship:t =2.414,P =0.017;depression:t =67.484,P =0.000;anxiety:t =8.198,P =0.000;hostile:t =26.366,P =0.000;paranoid:t =4.862,P =0.000;Psychotic:t =0.951,P =0.343).Conclusion Continuity self -management educa-tion has a positive effect on emotional states and quality of life,which is worthy of clinical promotion.

Since the tumor has become one of the most deadly diseases to human life ,it is of great impor-tance to explore a new effective antitumor medicine .This paper summarizes the chemical constituents and the mechanism of Inonotus obliquus .The mechanism includes the details of the inhibition of tumor cell proliferation , inducing tumor cell apoptosis ,influence tumor cell cycle etc .Meanwhile,it describes Inonotus obliquus to the ad-juvant chemotherapy of tumor .

OBJECTIVE:To provide reference for standardization of drug package.METHODS:5 dose-related items stated in a total of 426 package inserts of chemical drugs collected from the drug storeroom in our hospital were investigated and analyzed.RESULTS:Only 32.39% of the package inserts were with complete labeling.The labeling rate for special group was low and only 40% labeled the maximal dose.Less than 2% of child dose stated the doses according to body surface area,and the problem of over-dose was common.Only 27.46% had the item of "under supervision of a physician".CONCLUSION:It is necessary to strengthen supervision on drug package inserts and improve cognition on the importance of drug package inserts.

Objective To study the CT and MRI features of cystic brain metastases. Methods 26 cases of cystic brain metastases proved by clinicopathology were presented.There were13 males and 13 females with an average age of 52.7 years.CT studies were obtained in 15 cases,while MR studies were done in 13 cases.11 cases had CT and/or MRI contrast enhancement studies. Results According to the shape of the wall of cystic brain metastases,48 tumors in 26 cases were classified into three types: irregular,nodular,regular type.The wall demonstrated iso-or slightly high density on CT and iso-or slightly low intensity on MR T_1WI and T_2WI,while the hydatid fluid showed slightly high intensity on MR T_1WI and high intensity on T_2WI and iso-or slightly high density on CT compared to CSF.The tumors showed circular reinforcement on enhanced CT or MRI,with nodular enhancement in some cases. Conclusion Cystic brain metastases have mutiple types and should be differentiated from brain abcess and other cystic brain parenchamal tumors.Multi-direction enhanced MRI is helpful to diagnosis.

Objective To study the relationship between the mutation of androgen receptor(AR) gene and the development of human prostat e cancer. Methods All exons of AR gene except trineucl eic acid repeat polymorphism were amplified by PCR and selected by single strand conformation polymorphism( SSCP) in 27 paraffin-embedded slides of human pros tate cancer. The abnormal mobility shifts were further detected by direct DNA c ycle sequencing. Results Three abnormal mobility shifts respectively in exon A.D.G were found by SSCP in three prostate cance r tissues. One point mutation was found in 296 amino acid (C 966→A,Ser29 6Arg) from exon A.This point mutation has not been reported before. Conclusions This mutation might change the transcriptive activity of AR, and it might be correlated to PCa progression.

Objective To study the difference between central and peripheral position of contrast gray-scale imaging in rabbit′s liver.Methods Ultrasound contrast agent(UCA) SonoVue TM ( 0.1 ml/kg) was applied respectively in 8 normal rabbits by intravenous bolus injection.Corresponding parameters of the time-intensity curve: time to enhancement(ET),time to peak intensity(PIT),peak signal intensity(PSI),mean transit time(MTT) and area under the curve(AUC) were measured using low mechanical index contrast gray-scale imaging and Wash-in/Wash-out time intensity curve software.Results There was significant difference of parameters of the time-intensity curve between central position and peripheral position of parenchyma ( P

Objective To study the relationship between doses of ultrasound contrast agent(UCA) and contrast-enhancement effects of gray-scale imaging in rabbit liver. Methods Ultrasound contrast agent SonoVue TM in eight different doses ( 0.02 - 0.16 ml/kg) was applied respectively in five normal rabbits in randomized order as a bolus in a marginal ear vein followed by a 1.5 ml saline flush. Corresponding parameters of the time-intensity curve: time to enhancement(ET),time to peak intensity(PIT),peak signal intensity(PSI),mean transit time(MTT) and area under the curve(AUC) were measured using low mechanical index contrast gray-scale imaging and Wash-in/Wash-out time intensity curve software package. Results Along with an increase of the SonoVue TM dose,the MTT and the AUC increased exponentially,and the ET decreased exponentially. The PSI of portal vein and liver parenchyma increased only in the dose range from 0.02 ml/kg to 0.10 ml/kg ( r = 0.89 and 0.92 ,respectively),and decreased with a further increase of the UCA dose. A possible explanation for this effect is the bubble saturation and attenuation. No significant correlation was found between the UCA dose and the PIT( r = -0.35 and 0.22 ,respectively). Conclusions The parameters of time-intensity curve are influenced intensely by different UCA dose. It is important to grasp the relationship between the UCA doses and contrast-enhancement effects for microcirculatory quantification. The 0.10 ml/kg appears to be the optimal dose for tissue perfusion studying because it significantly improved videointensity and duration of enhancement and did not result in the bubble saturation and attenuation.