ObjectiveTo study the influences of fetal umbilical cord around neck on foetus、newborn infants and the choices of delivery mode during delivery.Methods220 cases of cephalic delivery pregnant women with fetus who had fetal umbilical cord around neck symptom and 220 cases of cephalic delivery pregnant women with fetus who didn't have fetal umbilical cord around neck symptom,were selected to make a contrast observation.ResultsThe foetus who had fetal umbilical cord around neck symptom had higher rate of fetal distress and neonatal asphyxia.The maternal cesarean section would be much higher,too.The rate is 18.5％,8.6％,49.6％ which were higher than the foetus who didn't have such kind of symptom,the rate is 5.4％,1.8 ％,26.4％ ( all P ＜ 0.05 ).The rate of fetal distress and neonatal asphyxia was proportional to the number of circles around neck.One circle around neck is 12.6％,1.4％ ;two circles around neck is 27.1％,17.0％ ;three and more than 3 circles around neck is 69.2％,53.8％.ConclusionWe should enhance perinatal B-mode ultrasonic examination and fetal monitoring circle around neck,pay close attention to the factor of umbilical cord around neck and select suitable delivery mode so that we can reduce the incidence of fetal distress and neonatal asphyxia.When B-mode ultrasonics displays that the number of the umbilical cord around the fetus' neck is three and more than 3 circles,the incidence of neonatal asphyxia is high and the cesarean section is the best choice under such circumstances.

Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year; However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-protein(EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115.The EV71 P1 pretein with a molecular weight of 100 kD was produced and secreted into the medium.The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70％.The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits.We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.

Objective To investigate the mechanism of glucocorticoid resistance in patients with systemic lupus erythematosus(SLE) and the effects of Panax Notoginseng Saponins(PNS) on reversing glucocorticoid resistance in lymphocytes. Methods The relevance between P-glycoprotein (P-gp, %) and SLEDAI integrals or clinical effects was analyzed by linear correlation analysis. The lymphocytes from SLE patients were intervened with PNS or verapamil. P-gp of the lymphocytes and Rhodamine 123 (Rh123, %) accumulated in the lymphocytes were assayed by flow cytometry. The percentage of the apoptosis of the lymphocytes stimulated with PNS or verapamil combined with methylprednisolone was identified by double-tagging with Annexin V and propidium iodide (PI) and detected by flow cytometry. Variance analysis or Student's t test was used for statistics. Results The P-gp in the peripheral blood lymphocytes of SLE patients was significantly related to SLEDAI integrals. The difference of P-gp between lymphocytes in the completely remission cases (12.2±2.5)% and lymphocytes in the partial remission or non-effective cases (16.5±4.0)% was statistically significantce. The difference of P-gp and Rh123 between lymphocytes treated with PNS [(11.2±3.1)%,(70.1 ±5.8)% respectively ] and lymphocytes of the control group [ (15.3±2.9)%, (53.9±5.2)% respectively ]was statistically significant. The lymphocytes apoptosis rate in the lymphocytes stimulated with methylprednisone combined with PNS increased significantly compared to the lymphocytes stimulated with methylprednisolone only. Conclusion The action of PNS in suppressing both the expression and the transfer activity of P-gp is possibly the important mechanism to increase the effects of methylprednisolone in inducing lymphocytes apoptosis.

OBJECTIVE:To compare the extraction yield of total flavonoids from fine powder vs.ultrafine powder of Gleditsia sinersis.METHODS:The absorbance was detected under a wavelength of 510 nm by ultraviolet spectrophotometry. The extraction rates of flavonoids from fine powder vs.ultrafine powder of Gleditsia sinersis were computed.RESULTS:The extraction rate of flavonoids from ultrafine powder was 29.1%higher than that from the fine powder of Gleditsia sinersis. CONCLUSION:The extraction rate of flavonoids was increased by superfine comminution.

Objective To investigate the expression of carbohrdyate antigen (CA153 ) and carcinoembryanic antigen(CEA) ,and itsclinical significance in the patients with breast cancer.Methods The sera CA153,CEA levels were determined by IMX in 30 healthypersons,52 patients with benign breast diseases and 208 patients with breast cancer from 1998 to 2002 ,the correlation between the CA153,CEA level and the clinical characteristics was also detected.Results In the patients with breast cancer, the level of serum CA153 and CEA was significant higher in pre-treatment than that in post-treatment(P

Objective To explore the mechanism of the inflammatory factors to increase the reanl inferstitial fibrosis by stndying on epithelial-to-mesenchymal transition induced by IL-1?.Methods Mice primary tubular epithelial cells were Cultured in vitro,then stimulated by IL-1? for 3,4,5 days respectively,from which the protein was extracted and the protein expression of ?-SMA and Vimentin was detected with western bloHing;while the cells were stimnlated by IL-1? for 12hs,36hs and 60hs respectively,from which then total RNA was extracted and the mRNA expression of ?-SMA and Vimentin was detected by ranscription-polymerase chain reaction analyses(RT-PCR).those without IL-1?stimulating were regarded as control group.Results The protein levels of ?-SMA,Vimentin in tubular epithelial cells stimulated with IL-1?for 3 days or 4 days were significantly higher than that in normal group cells.Those stimulated for 5 days,the protein levels of ?-SMA,Vimentin in tubular epithelial cells increased very high.The mRNA expression of ?-SMA and Vimentin increased significantly in 12hs in tubular epithelial cells after IL-1?stimulating as compared with normal group cells and presented time-depend.Conclusions IL-1? plays the role of promoting renal interstitial fibrosis by inducing the transdifferentiation of renal tubular epithelial cells and reducing the proliferation cells probably.

Objective To explore the 5,10-methylenetetrahydrofolate reductase( MTHFR) and 5-methyltet-rahydrofolate-homocysteine methyltransferase reductase( MTRR) gene polymorphisms among the Han women in Laiwu City.Methods A total of 559 Han women were recruited.And their oral epithelial cells were collected to extract genome DNA in order to detect gene polymorphisms of MTHFR and MTRR using fluorescence quantitative PCR.Then the results were compared with those in other cities in China.Results The frequency of MTHFR 677CC,677CT and 677TT of Han women in Laiwu city was 14.3%,46.7%and 38.1%,respectively.The frequency of MTHFR 677TT among Laiwu women was significantly different to those of Zhenjiang, Wuhan, Kunming, Deyang, Huizhou, Qionghai (P<0.05).The frequency of AA,AC,CC gene type on MTHFR A1298C was 78.2%,19.7% and 2.1%,respec-tively, the frequency was significantly different to those of Zhenjiang, Wuhan, Kunming, Deyang, Huizhou, Qionghai (P<0.05).The frequency of AA,AG,GG gene type on MTRR A66G was 53.3%,38.8%and 7.9%,respectively. The frequency of MTRR 66GG was significantly different to that of Qionghai(P<0.05).Conclusion The MTHFR, MTRR polymorphism distribution of Han women in Laiwu City has the characteristic of region specificity,respectively.

Objective To investigate the ex pr ession of squamous cell carcinoma antigen (SCC-Ag) and carcinoembryanic antigen (CEA) and its clinical significance in patients with lung cancer. Meth ods The pre-treatment serum from 134 patients with lung cancer from 1998 to 2002 were analyzed for the SCC-Ag level and CEA level by IMX, and the correlation of the SCC-Ag level and CEA level with the clinicopathologic charac teristics were also detected. Results Significant correlations were found between the pre -treatment SCC-Ag level, and CEA level and the pathologic classification, clin ical stage, the positive rate of diagnosing lung cancer was raised by combined a ssay with serum SCC-Ag and CEA. Conclusion Pre-treatment and after-treatment measurement of serum SCC-Ag level and CEA level can be used for diagnosis, prediction of pr ognosis and monitor of after-treatment recurrence in patients with lung cancer.

Objective To investigate the clinical significa nc e and the relationship between the serum squamous cell carcinoma antigen (SCC-A g) levels and the biological characteristics in patients with cervical carcinoma . Methods The pre-post-treatment sera from 500 patients w ith cervical carcinoma from 1998 to 2002 were analyzed for the SCC-Ag levels by IMX; and the correlation between the SCC-Ag level and the clinicopathologic ch aracteristics were also detected. Results Significant corre lation was found between the pre-treatment SCC-Ag level and pathologic classif ication, and clinical stage (P0.05); The pre-treatm ent SCC-Ag level is significantly higher than that of post-treatment (P

Objective:To evaluate the effect of humanistic care for medical care integration on quality of life in elder patients with mild cognitive impairment. Method:Seventy patients with mild cognitive impairment in our hos-pital were randomly divided into experimental group ( n=35 ) and control group ( n=35 ) using random number ta-ble. The patients in the control group were given routine care, while those in the experimental group were given hu-manistic care for medical care integration and home visiting once a month besides routine care. All the patients were evaluated by the Montreal Cognitive Assessment Scale ( MoCA) , Activities of Daily Living ( ADL) , and the MOS Item Short from Health Survey ( SF-36 ) during hospitalization, three months and six months after hospital discharge. Result:Three and six months after hospital discharge, the scores of these three scales of all subjects in two groups were significantly higher than those during hospitalization ( P<0 . 05 ) . The scores of the subjects in the experimental group also significantly higher than those of the control group, and the differences were statistically significant (P<0. 05). Conclusion: For the elder patients with mild cognitive impairment, humanistic care for medical care integration can significantly improve the cognitive function, self -care ability and quality of life. Therefore, it is worthy using in clinical treatment.