Objective This study evaluated whether liver ischemic preconditioning has delayed protective effect on liver ischemia-reperfusion injury and probe into the protective mechanism.Methods 36 wistar rats were randomly divided into two groups, group A:ischemic preconditioning,Group B:Control. The group A rats were subjected to liver ischemic preconditioning,While group B rats were subjected only laparotomy.After 48 hours,Liver tissue samples were abstained to detect the expression of HSP70.Occlusion the inflow vessels to the left and median lobes 45-minute then reperfuse 90-minute.Liver tissue samples were abstained to detect the expression of TNF-?.Serum ALT was detected before ischemia and after 90-minute reperfusion.Results The serum ALT level in group A is much lower than that in group B (P

OBJECTIVE:To effectuate the pharmacy information communication with in the hospital.METHODS:The local area network concerning medicine information in the hospital was built by adopting FrontPage and Dreamweaver with the assists of Flash,Photoshop and Fireworks so as to provide services for the communication of pharmaceutical sciences informa-tion and the information collecting.RESULTS:The website on medicine information has been operating normally for11months and the visitors to which were more than5000person-time.CONCLUSION:Medicine information website has provided an active,healthy and an upward platform of communication and learning for all medical workers within the local area network of the hospital,which has make the gathering of clinical pharmacy information more promptly and effectively and which has achieve the expected effect.

OBJECTIVE:To study pharmacokinetics of domestic and imported Phenazopyridine hydrochloride tables and bioavailability of domestic tablets, and to evaluate the bioequivalence of two kinds of tablets. METHODS: A randomized crossover design was performed in 18 healthy male volunteers. They received a single oral dose of domestic or imported tablets 200 mg. Plasma concentration of phenazopyridine hydrochloride was measured by HPLC. The pharmacokinetic parameters were calculated by 3p97 software and relative bioavailability was evaluated. RESULTS: The plasma concentration-time curves of domestic and imported tablets conformed to one-compartment model. Main pharmacokinetic parameters of domestic tablets vs. imported tablets were as follows: t1/2Ke(3.52?2.03) h vs. (3.18?1.85)h; tmax(0.76?0.33) h vs. (0.79?0.43)h; Cmax(76.41?70.15) ng?mL-1 vs. (75.49?70.37) ng?mL-1; AUC0～8(159.10?116.32) ng?h?mL-1 vs. (164.65?129.89) ng?h?mL-1; AUC0～∞(237.12?115.06) ng?h?mL-1 vs. (262.69?155.05) ng?h?mL-1. The relative bioavailability of domestic tablets was (96.63?14.05)% compared with imported tablet. There was no significant difference between the pharmacokinetic parameters of two formulations by variance analysis, t-test and 1-2? confidence interval method. CONCLUSION: The domestic and imported tablets are bioequivalent.

Objective To investigate renal interstitial fibrosis and the expression of inflammatory factor in mice kedney with the Unilateral Ureteral Obstruction(UUO).Methods Mice were randomly assigned to shame operation group (shame proup ), UUO group. The protein expression of?-SMA and Vimentin were assessed by immunohistochemistry and the protein expression of ICAM-1 were assessed by Western-blotting; MCP-1 mRNA were detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR). Results In comparison with the shame group, the mRNA expression, protein levels of ?-SMA, Vimentin of UUO mice groups increased significantly. At the same timre, the protein expression ICAM-1 and the mRNA expression MCP-1 in UUO mice significantly higher than normal group.Conclusions Unilateral ureteral obstruction could induce the transdifferentiation of renal interstitial cells. It may partially related to the high expression of ICAM-1and MCP-1.

Weight gain, skinfold thickness, circumference and body density were measured in 30 primiparous women aged from. 23-31 years (averaged 26.3 years old).The average body weight gained 13kg from 6 to 37 weeks of pregnancy.The suprailiac skinfold thickness in 24-37 weeks and the subscapular skinfold thickness in 37 weeks increased significantly from the 6th week of pregnancy, but the triceps and biceps skinfold thickness did not change significantly. The density of the body in 24th and 37th week of pregnancy decreased significantly.The change and the body fat (%) calculated by means of 4 skinfolds increased in 3.7% (5.7kg of fat). The mean values of the hip circumference in 24th and 37th week were significantly different from that in 6th week of pregnancy. The upper thigh in the 24th and 37th week also increased slightly but not significantly different as compared with the 6th week. The mean values of arm, mid-thigh and calf circumference did not change during pregnancy.

Objective:To evaluate the antimicrobial effectiveness of Zhenzhu Mingmu eye drops according to the requirements de-scribed in Chinese pharmacopoeia (2010 edition). Methods:Totally 7 batches of Zhenzhu Mingmu eye drops from 7 enterprises were evaluated. All the samples were tested according to the requirements described in Chinese pharmacopoeia, and the testing points of 6h and 24h were added. Results:As for bacteria, the reduction value of staphylococcus aureus in 7 days treated with Zhenzhu Mingmu eye drops from enterprise e was 0. 61g, and that in 14 days was 1. 01g. That in 7 days treated with the eye drops from the other enter-prises was all above 1. 01g, that in 14 days was above 3. 01g, and that in 14-28 days kept stable. As for fungi, the number was stable from the beginning with the treatment of the eye drops from the seven enterprises. Conclusion:Although the type and content of bacte-riostatic agent in domastic Zhenzhu Mingmu eye drops are the same, there is difference in the antimicrobial effectiveness of the product from different enterprise. The eye drops from one enterprise can't meet the requirements for type I product described in Chinese pharma-copoeia,and the others meet the requirements.

Despite seminal studies in the 1920s by Warburg showing a characteristic metabolic pattern for tumors, cancer bioenergetics has otfen been relegated to the backwaters of cancer biology. Recent studies have shown that metabolism in the tumor tissue is far more complicated than we previously knew. Despite vigorous glycolysis, in fact, the tumor tissue still retains mitochondrial aerobic metabolism. Mitochondria is one of the main sites of the biosynthesis process of tumor cells. Recent studies revealed that abnormal fatty acid metabolism. Amino acid metabolism plays a key role in the tumorigenesis. The metabolic network in tumor cells was reprogrammed, leading to nutrition lfux reorganization and re-direction. Metabolism reprogramming in tumor cells facilitates the balance between the needs of energy supply and the synthesis of biological macromolecules. Targeting cell metabolism is not intended to interfere the energy supply of tumor cells but to affect the synthesis of metabolic rate, thereby inhibiting the proliferation of the tumor. hTe review focuses on the importance of metabolic reprogramming in tumor development and cancer therapy. We summarize what is currently known about metabolic reprogramming and establish a framework to understanding its contribution to the altered metabolism of cancer cells.

Objective: To set a quantified diagnostic standard for large intestinal cancer of spleen qi deficiency syndrome. Methods: The spleen qi deficiency syndrome was identified by experts on the basis of clinical epidemiological investigation of 311 patients suffering from large intestinal cancer. Corresponding points were assigned to the correlative factors (traditional Chinese medicine symptoms) on the basis of symptom differences between spleen qi deficiency syndrome and non-spleen-qi-deficiency syndrome. The best threshold was determined by receiver operating characteristic curve (ROC) according to syndrome differentiation from expert team, and the quantified diagnostic standard was established. The syndrome identification from the expert team which was regarded as golden standard was tested retrospectively. Results: All the traditional Chinese medicine symptoms possibly related to spleen qi deficiency syndrome were analyzed based on the opinions of experts, and 28 symptoms were confirmed as candidate correlative factors. The occurrence of 11 symptoms between spleen qi deficiency syndrome and non-spleen-qi-deficiency syndrome showed statistical differences by means of crosstabs analysis (P<0.05). The 11 symptoms were filtered by logistic regression analysis, and tiredness, fatigue, loose stool, and poor appetite were finally determined as the symptoms relative to large intestinal cancer. These four symptoms were analyzed with conditional probability conversion and endowed with 16, 11, 4 and 8 points respectively. The diagnostic standard of spleen qi deficiency syndrome of large intestinal cancer was over 13 points. The sensitivity, specificity and accuracy of retrospective examination were all above 80%, and its positive likelihood ratio was 9.89. Conclusion: The quantified diagnostic standard for spleen qi deficiency syndrome of large intestinal cancer is in accordance with clinical characteristics of large intestine cancer and the characteristics of TCM syndrome diagnosis.

OBJECTIVE:To provide a reference for reducing adverse events due to off-label use of drugs and promoting ratio-nal use of intravenous drugs in Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine. METHODS:The pre-scriptions of intravenous drugs in 42 wards of our hospital from Jul. 2013 to Jun. 2014 were reviewed,and then off-label use of drugs were analyzed,where statistics was made mainly to drug overdose,the use of drugs at the concentration beyond the standards (including concentrations too high and too low) and associated conditions. RESULTS:There were 622 irrational prescriptions,in-cluding 60 concerning off-label use of drugs,accounting for 9.6% of irrational prescriptions,in which 40 were involved with the concentration which was too high,accounting for the highest percentage(66.7% of prescriptions related to off-label use of drugs), 17 were related to drug overdose(28.3% thereof),and 3 were in relation to the concentration which was too low(5.0% thereof). CONCLUSIONS:Pharmacy intravenous admixture service may,through the communication between the pharmacist and the doc-tor,refuse to make up the above-mentioned prescription which was found to be irrational(including that involving in off-label use of drugs)or make up such prescription after it is modified according to the requirements as specified in the label to reduce the occur-rence of off-label use of drugs so as to promote rational drug use.

This study was aimed to summarize main-bone structure of cantharidin-based small molecules and facts about three typical candidates including its origin, physical-chemical properties and general synthetic approaches. Basic chemical and pharmacological information as well as development of anti-cancer activities, which were related to cantharidin, norcantharidin and their analogues were reviewed, especially the relationship between the structures and their inhibitory activity and selectivity of serine-threonine protein phosphatases PP1, PP2A and PP2B in cancer treatment. The designs and developments of new biological cantharidin-based small molecules were also reviewed.