PurposeTo investigate the permeation of model drug,propranolol hydrochloride, through the excised porcine buccal mucosa. To study the influences of various factors on the buccal absorption also studied. MethodsUsing excised porcine buccal mucosa permeation test in vitro. By means of changing the medium pH, osmotic concentration, drug concentration, the concentration of various penetration enhancers including sodium glycodeoxycholate (GDC), sodium glycocholate (GC), dimethy1-β-cyclodextrin (DMβCD), and hydropropyl-β-cyclodextrin(HPβCD). The permeation rates and permeability coefficients(P) were determined and evaluated. The solubilities and apparent partition coefficients also determined in some tests. ResultsThe result showed that the P were increased when porcine buccal mucosae were pretreated with every penetration enhancement of certain concentration, increased as the pH of test solution rose from 4.9 to 9.1. The P remained constant as the concentration of propranolol hydrochloride increased from 5 to 80 mg/ml, decreased with the increasing of osmotic concentration Conclusions The absorption of propranolol hydrochloride through porcine buccal mucosa is a typical passive process, and affected by penetration enhancers, medium pH, osmotic concentration.

Objective: To explore the effect of health education and supportive therapy on neuroses. Methods: Fifty six subjects were assigned into two groups according to the sequence of hospitalization. Both groups received the same drug treatment, average dosage: in experiment group fluoxetine 20～30mg/d (9 cases), Amitriptyline 75～150mg/d(8 case), Alprazolam 1.6～ 2.8mg/d(13 cases), received the health education and supportive therapy for 38 to 60 days in addition to drug treatment. In control group, fluoxetine 20～30mg/d(11 case), Amitriptyline 75～200mg/d(5 cases), Alprazolam 1.2～2.8mg/d(10 cases) were used. The Symptom Checklist 90 was used for assessment before and after treatment and cure rate was evaluated by clinical standard at discharge in both groups. Results: Cure rate was higher in the experimental group (94%) than control group (71%, P<0.05). The total score of the Symptom Checklist 90 and main factors were lower in the experimental group than the control group (P<0.01). Conclussion: Early health education and supportive therapy can improve the treatment outcome of patients with neuroses.

Objective　To study the diagnostic value of pleural fluid telomerase activity in distinguishing a malignant from a benign pleural effusion. Methods　Using a PCR-based assay, telomerase activity was examined in the pleural fluid cells obstained from 69 patients with pleural effusions (37 malignant, 32 benign). Results were compared with cytologic evaluation and carcinoembryonic antigen (CEA). Results　Of the 37 malignant specimens，26（70.27%）contained detectable telomerase activity. The positive rate in malignant pleural effusions was significantly higher than that in benign origin (2/32). Detection of telomerase in effusions was more sensitive than CEA level evaluation for the identification of pleural malignancy (telomerase assay: sensitivity=70.27%, specificity=93.75%; CEA: sensitivity=51.35%, specificity=96.87%). The consistant rate of telomerase with cytology was 54.05%. Conclusion　The detection of telomerase activity may be a useful adjunct to cytopathologic methods in the diagnosis of malignant pleural effusions. It may be useful in differential diagnosis of benign and malignant pleural effusions.

The author analyzed current situtation of teachers and students during clinical practice,and proposed that we should well attach importance to the training of behaviour,including working style,basic skill,clinical thinking and research idea,to improve the quality of medical education in clinical teaching,

Objective Seeking for a new therapy for effectively correc ting hypoxemia of severe hepatitis cases in terms of oxygen-enriched liquids in f usion, for a rapid boost of the pressure of blood oxygen bypassing the pathologi cally damaged alveolus of such patients.Method Intravenous drip with oxygen -enriched liquids for 23 cases who suffered from Severe hepatitis in courses of tre atment varying between 14 to 30 days, 500-700 mL per day.Result For all the 23 c ases, their hypoxemia was gradually corrected to normal in 20 minutes' or 7 hour s du ring intravenous drip with oxygen-enriched liquids and TNF ?IL-6?IL-8 were dec r eased after the treatment courses.The death rate of treatment group was l ess.Conclusion Intravenous drip the oxygen-enriched liquids has effectively helped to corre ct the hypoxemia of severe hepatitis cases bypassing the malady alveolus throug h which the oxygen failed in by conventional oxygen halation. Death rate hase been decreased after the courses .This therapy of oxygen-enriched liquid infusion can be quite useful to save their life in the combined treatment for severe hepatitis.

Objective To investigate the occupational hazards of nurses in the supply room,to determine what kind of occupational hazards is most dangerous,and accordingly put forward some suggestions of protective measures.Methods Totally 208 nurses from supply rooms of 4 general hospitals were surveyed with questionnaires.The data of occupational hazards were analyzed.Results Various kinds of occupational hazards happened among nurses in the supply room,and among all occupational hazards,physical damage was the most frequently happened.Conclusions The occupational protection awareness of nurses is relatively weak.They can not properly handle damages caused by occupational hazards,so sufficient attention should be given to it.

Based on the analysis of the status quo of computer teaching,the present paper discusses the reform of teaching contents in the computer education in our college.

Problem-Based Learning(PBL)is a new teaching mode.Application of PBL in Laboratory Diagnostics is a practical teaching reform.Students'learning mainly by themselves is PBL’s main feature.The status and role of teachers have a qualitative change.They are not teaching but guiding.Therefore teachers must realize the change of their roles in PBL.

Objective To analyse clinical effects of Juming Jiangya pill combined with amlodipine besylate in treatment of essential hypertension.Methods 126 patients with essential hypertension from August 2015 to October 2016 were grouped two groups and each with 63 cases.The control group were treated with amlodipine besylate,and observation group was treated with Juming Jiangya pill.The effect of 2 groups of antihypertensive treatment, the influence on related factors and the safety were analyzed.Results (After treatment,total effective rate of observation group was 95.24%,higher than that of control group 80.95%(P<0.05).(After treatment,SBP and DBP level of observation group[were(128.5±6.3),(78.4±5.2)mmHg],lower than that of control group[(140.2±7.5),(88.7±5.5)mmHg](all P<0.05).③After treatment,plasma NO and serum ET level of observation group were[(70.16±5.51)μmol/L,(66.24±5.40)ng/L],better than that of control group[(64.16±5.33)μmol/L,(73.05±5.68)ng/L)](all P<0.05).④During treatment,proportion of adverse reactions of control group was 11.11%,higher than that of observation group 4.76%,difference was not statistically significant.Conclusion Juming Jiangya pill combined with amlodipine besylate have better curative effect and medication safety.

Objective To explore the smoking cessation intervention impact on lung with genetic polymorphism of SOD3. The aim is to further reveal the importance of smoking cessation intervention on early COPD patients. Meth-ods 60 COPD patients with smoking cessation intervention and 40 COPD patients without intervention ( the control group) were enrolled in this study. Limosis peripheral blood was taken and whole blood corpuscle genomic DNA was extracted. The genetic polymorphism of SOD3 genes was determined by ligase detection reaction and polymerase chain reaction ( LDR-PCR) and serum SOD3 concentration was measured using ELISA. Lung function between the two groups before and after intervention were detected by microspirometry. Results ①The FEV1% and the FEV1/FVC% were increased after 3 and 6 months intervention in smoking cessation group(P<0.05). But there was no significant difference ( P <0.05 ) between 3 and 6 months. ② Different SOD3 genotypes have no significant on COPD pulmonary function after intervention(P>0.05). ③The serum of SOD3 concentration of COPD intervention group and the control have no significance after intervention ( P>0.05 ) . ④ The serum of SOD3 concentration a-bout CC and CG/GG genotype in COPD intervention group have no significance after 3 and 6 months intervention ( P>0.05 ) . Conclusion Smoking cessation interventions for patients with COPD pulmonary function improves significance in short-term. But different SOD3 genotypes have no effect on lung function after intervention. Smoking cessation intervention has no effect on in serum SOD3 concentration of COPD patients, and has no relationship be-tween the expression of SOD3 genetype.