Object To prepare Panax notoginseng saponin (PNS) liposomes and investigate their characterization of pharmaceutics and pulmonary pharmacokinetics in rats. Methods The film-disperion method was used to prepare PNS liposomes by investigating its form, vesicle size, entrapping efficiency and stability. The study of pulmonary pharmacokinetics in rats was carried out by pulmonary instillation. Results The entrapment of PNS liposomes was 78.50%. The mean vesicle size was 1.5 ?m with uniform externality. The drug leakage from PNS liposomes was slow. Low temperature was required for its storage. The pulmonary pharmacokinetics parameters were as follows: T 1/2 ?=7.00 h, T 1/2 ?=27.72 h, AUC= 2 218.9 ?g?h/mL, the absolute bioavailability was 70.14%. Conclusion PNS liposomes with high entrapment and stability can prolong its circulation in blood and improve the PNS bioavailability via lung approach.

AIM: To study the preparation and physiological compatibility of pulmonary administration with Saponius of Panax Notoginseng (PNS) liposomes. METHODS: PNS liposomes was prepared by thin film dispersion method. The prefered formulation was determined by factorial design. Ciliary movement in situtoad palate model and the changes of pulmonary edema index and pathology of lungs in rats were investigated to evaluate the physiological compatibility of PNS liposomes. RESULTS: PNS liposomes were proved to be large unilamellar vesicle (LUV) liposomes with spherical shape .The liposomes composed of PC and Ch has a high PNS capture efficiency of 78.5% ?2.15% . The mean vesicle size of liposomes were 1546.5nm ?321.4nm which were quite homogeneous in size distribution. The lasting time of ciliary movement after the rinsing of PNS liposomes was the 92.14% of the effect of PBS buffer. The pulmonary edema index and pathological changes of lung tissue in PNS liposomes group were significantly different from those in PNS solution group and close to those in PBS buffer group. CONCLUSION: PNS liposomes has good physiological compatibility with promising market development and application.

Objective This study was designed to investigate the relationship between COX-2 and VEGF-C in human gastric carcinomas and discuss the significance of COX-2、VEGF-C in lymph mode metastasis.Methods Forty-two gastric carcinoma tissues and twenty adjacent noncancerous specimens were obtained from surgical resections.COX-2,VEGF-C protein expression was evaluated by immunohistochemical analysis.Results (1)COX-2 was overexpressed in 25 of 42 carcinoma tissues (59.5%) and VEGF-C was 27 of 42(64.3%),which were significantly higher than those in noncancerous tissues (P0.05).(3)There was positive correlation between the expression of COX-2 and VEGF-C(r=0.425,P

BACKGROUND:Guidances for design of Chinese people’s temporomandibular joint prosthesis are urgently needed because foreign prosthesis is unsuitable for Chinese people due to variant anatomy parameters.
OBJECTIVE:To provide a reference for improving the design of artificial temporomandibular joint through computer three-dimensional digital measures of the anatomy parameters of the temporomandibular joint. METHODS:Continuous CT data with layer thick 0.625 mm of 30 normal temporomandibular joints were selected. Three-dimensional digital reconstruction and eight related anatomy parameter measurements of the articular fossaandcondylar of the joints were performed using Mimics10.01 software.
RESULTS AND CONCLUSION:The internal and external diameter of the mandibular fossa, head of mandible and neck of mandible were greater thantheanterior and posteriordiameter. The mandibular foramen was located at middle-posterior of the ramus ofthe mandible. The distance between head of the mandible and coronoid process was longer than the depth of the mandibular notch. There were no
significant differences between two sidesregardingal above-mentioned parameters. There are certain regular patterns in anatomy parameters of the temporomandibular joint. The study results wil provide a reference for improving the design of artificial temporomandibular joint.

Objective To understand the current situation of dispensary setting and personnel of community health service centers in Pudong New District in Shanghai (hereinafter referred to as Pudong New District).Methods Questionnaire survey covering the 45 community health service centers in Pudong New District was used.Results 42 community health service centers were equipped with TCM dispensaries among the 45 total units (93.3%);12 of them adopted outsourcing mode (26.7%);the average area of community dispensary was (32.3 ± 17.3)m2;the average number of TCM practitioners was 3.5 ± 2.0 people.Conclusion Community health service centers need to increase the allocation area of TCM dispensary and improve qualification of TCM practitioners.

OBJECTIVE:To evaluate the safety and tolerance of magnesium isoglycyrrhizinate(MgIG)injection in Chinese healthy volunteers,and provide safe and utility dosage scheme.METHODS:36healthy volunteers were randomly divided into4groups,each group administered respectively with single-dose intravenous infusion of100,200,300mg of MgIG and mul?ti-dose intravenous infusion of200mg of MgIG,qd for7consecutive days.Clinical symptoms,vital signs,electrocardiograms,routine blood and urine tests,as well as hepatic and renal function were observed before and after IV infusion of MgIG.RESULTS:In single-dose IV infusion of MgIG groups,the vital signs and laboratory indices did not change in the subjects immediately after or at1h,8h,24h after administration,as compared with before,while in multi-dose constant intravenous infusion group,the vital signs and laboratory indices at4days and8days after administration were in the normal range,as compared with before.CONCLUSION:Tolerance of healthy subjects to MgIG is good,and daily dosage of100mg to200mg is recommended for clinical use.

OBJECTIVE To investigate the etiology of acute respiratory tract infection(ARI)in Hefei area and risk factors that may influence the distribution of common pathogens.METHODS Direct immunofluorescence assay was applied to detect the respiratory syncytial virus(RSV),adenovirus(ADV),influenza virus A(FluA),influenza virus B(FluB),parainfluenza virus PIV(1,2,3)and real time fluorescent quantitation polymerase chain reaction (FQ-PCR)was applied to measuring Mycoplasma pneumoniae(MP)and Chlamydia pneumoniae(CP)in nasopharyngeal secretions and sputum specimens.RESULTS Among 530 cases included in this study,421 cases (79.43%)showed positive viral etiology.ADV accounted for 73(13.77%),FluA-68(12.83%),FluB-56 (10.56%),RSV-48(9.05%),PIVl-47(8.86%),PIV3-42(7.92%),PIV2-33(6.22%),MP-32(6.03%)and CP-22(4.15%).The detected positive rate of pathogens isolated in winter was the highest(85.07%).CONCLUSIONS Acute upper respiratory tract infection(AURTI)is common and more than 75%pathogens in Hefei area are virus in which the most commonly virus is ADV.Meanwhile atypical pathogens of infection should not be ignored.

The physicochemical environment and action are similar between the traditional decoction and the extract technics with water or alcohol in the production of Chinese patent drug. Different heating time inevitably differs Chinese patent drug from its decoction; and the alteration of extracting dissolvent make great changes in the chemical constitution. All these lead to the change in the nature of a Chinese patent drug. The authors hold that it is difficult to embody exactly the aim of the prescription of Chinese drug in the existing production technology of Chinese patent drug. It is necessary to advance innovative thoughts of adopting modern technology to extract effective ingredients from single Chinese drug and in the reference of traditional decoction, recombining the composition and dosage of Chinese patent drug.

AIM:To investigate the effect of ERK1/2/c-Fos signal pathway during angiotensin-(1-7)inhibiting proliferation of rat glomerular mesangial cell strain(GMCS)induced by angiotensin Ⅱ.METHODS:Rat glomerular mesangial cells(GMC)were co-cultured with angiotensin Ⅱ and different doses of angiotensin-(1-7).The numbers of GMC were evaluated by crystal violet staining.The amounts of p-ERK1/2 and c-Fos expressions were detected by Western blotting.RESULTS:Angiotensin-(1-7)showed its inhibitory effects on GMC number increasing induced by angiotensin Ⅱ as well as the amounts of p-ERK1/2 and c-Fos expressions in a concentration dependent manner.CONCLUSION:ERK/c-Fos signal pathway is involved in the inhibitory effects of angiotensin-(1-7)on angiotensin Ⅱ-induced GMC proliferation.

Objective To investigate the effect of angiotensin-(1-7) on the expression of cellular c-fos in angiotensin II -induced proliferative glomerular mesangial cells (GMC). Methods GMC were treated with angiotensin II and different dose of angiotensin-(1-7). GMC number were evaluated by crystal violet staining and the expression of c-foe were detected by western blot. Results Angiotensin-(1-7) inhibit angiotensin II -induced GMC proliferation as well as the expression c-foe in a concentration dependent manner. Conclusion c-fos is involved in the inhibiting effects of angiotensin-(1-7) on angiotensin II -induced GMC proliferation.