1.Treatment of 60 Cases of Simple Obesity by Acupuncture plus Tuina Therapy
Xiaoli SHANG ; Xiaoling SHANG ; Guoqi HUANG
Journal of Acupuncture and Tuina Science 2003;1(3):42-44
Purpose To observe the therapeutic effect of acupuncture therapy plus Tuina therapy in the treatment of simple obesity. Methods Ninety-eight cases of the patients were randomly divided into the two groups and 60 cases in the treatment group were treated with acupuncture therapy plus Tuina therapy, and 38 cases in the control group were treated with single acupuncture therapy. Results The effective rate was 100% in the treatment group and was 71.0% in the control group, P < 0. 01. Conclusion Acupuncture therapy plus Tuina therapy is better than single acupuncture therapy in the treatment of simple obesity.
2.Role of haemophilus influenza factor in the process of biofilm formation
Xue GAO ; Xiaoling SHANG ; Yutuo ZHANG
Tianjin Medical Journal 2015;(3):330-332,333
The Haemophilus influenzae (Hi) is a common opportunistic pathogen from human respiratory tract. The lower immunity can cause the disease. After the biofilm (BF) is formatted by Hi at the site of infection, the antibiotic sensitivity is declined, which often causes the chronic disease, inducing the difficulty in clinical treatment. In recent years, the pathogenic?ity of Hi BF and BF form related factors have gradually become the focus of clinical and basic research. This paper reviewed recent studies as following.
3.Effect of Ruditapes philippinarum diet on the development of experimental atherosclerosis in rabbits
Yanchang SHANG ; Chengming XING ; Xiaoling YUAN ;
Chinese Journal of Marine Drugs 1994;0(02):-
Objective To evaluate the effect of diets rich in Ruditapes philippinarum on the development of atherosclerosis. Methods The rabbits were fed with normal forage or high lipid diet with a daily supplement of Ruditapes philippinarum for 8 weeks respectively. Serum lipid, the area of atherosclerotic plaque and IMT were analysed.Results Compared with control group, serum lipid profiles didn't change obviously and atherosclerotic lesion was not observed in group fed with a supplement of RP. Compared with model group, the concentration of serum TC, TG and LDL C/HDL C decreased significantly, while the concentration of serum HDL C increased significantly in group fed with high lipid forage and a supplement of RP (p
4.Seven cases of laryngeal nerve paralysis secondary to benign thyroid lesion.
Xiaoling SHANG ; Yantao LIN ; Gang XUE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(24):1146-1146
Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Thyroid Diseases
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complications
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Vocal Cord Paralysis
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etiology
5.DNA methylation in thyroid carcinoma.
Xianyun SONG ; Xiaoling SHANG ; Yutuo ZHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(6):573-576
Cancer has become clear that not merely gene variations but also epigenetic modifications may contribute to it. Epigenetic changes refer to stable alterations in gene expression with unrelated to changes in the underlying genetic sequence,resulting in heritable. DNA methylation is one of the common epigenetic changes. It control the gene expression through changing DNA conformation and stability, chromatin structer, DNA-protein interaction. The reversal of dysregulated DNA methylation has emerged as a potential strategy for the treatment of thyroid carcinoma. The artical will provide an overview of how DNA methylation contribute to thyroid carcinoma dissemination,invasion and metastasis and we will summarize the latest epigenetic therapies for thyroid carcinoma.
Chromatin
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DNA Methylation
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Epigenesis, Genetic
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Gene Expression
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Humans
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Neoplasm Metastasis
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Thyroid Neoplasms
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genetics
6.Evaluating the change of left atrial function in the patients with paroxysmal atrial fibrillation underwent radiofrequency catheter ablation using echocardiography
Xiaoling ZHANG ; Hao RONG ; Yanwen SHANG ; Hong TANG
Chinese Journal of Ultrasonography 2014;23(4):293-296
Objective To evaluate the change of left atrial(LA) volume and function in patients with paroxysmal atrial fibrillation (PAF) after catheter ablation using real-time tri-plane echocardiography (RT-3PE) as well as quantitative tissue velocity imaging (QTVI).Methods Eighty-two PAF patients underwent first-time RFCA were included.Routine and tri-plane ultrasound images of 1 week before and 1 month,3 months,6 months after RFCA were stored for offline analysis.A range of LA structural and functional parameters were measured off-line using the EchoPAC workstation,including LA anteroposterior diameter (LADap),LA mediolateral diameter (LADml) and LA superior-infra diameter (LADsi),early and late diastolic transmitral flow velocity and ratio (E,A and E/A),the peak velocity of pulmonary vein and ratio (S,D,Ar,S/D),the maximum volume of the left atrium (LAVmax),the volume before LA active contraction obtained at time of the P wave on the surface electrocardiogram(LAVp),the minimum volume of the left atrium (LAVmin),LA passive emptying fraction (LAPEF),LA active emptying fraction (LAAEF),LA total emptying fraction (LATEF),LA expansion index (LAexpI),average peak velocity of six wall of the left atrium (Vs,Ve,Va).Results After 3,6 months,LADap decreased significantly(P < 0.05) ;3 months of follow up,peak A increased,E/A ratio decreased (P <0.05);6 months of follow up,peak A increased,E/A ratio reduced,peak S increased,peak D reduced,S/D increased(P <0.05) ;3 months of follow up,LAVmin reduced,LAAEF and LATEF increased (P <0.05);6 months of follow up,LA volume decreased,LAAEF and LATEF increased (P <0.05);3,6 months of follow up,the average Va increased(P <0.05).Conclusions LA reconstitution and function were recovering slowly 1 month after RFCA,and noticeable improvement can be observed 3 and 6 months after RFCA.
7.Biofilms produced by Haemophilus influenzae in vitro and antibiotics sensitivity changes
Xue GAO ; Xiaoling SHANG ; Haixia QIAO ; Yanxia ZHANG ; Cunhui ZHANG ; Yutuo ZHANG
Journal of Clinical Pediatrics 2014;(7):682-685
Objective To establish a bioiflm (BF) models of Haemophilus inlfuenza in vitro, and to observe the changes of antibiotic susceptibility after the BF fromation. Methods Thirty strains Haemophilus inlfuenzae isolated from adenoids of children with adenoidal hypertrophy and cultured in a 96-well plate. The BF was identiifed by crystal violet staining and scanning electron microscopy (SEM). The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the minimum bioiflm bacteria bioiflm clear concentration (MBEC) of ampicillin (AMP), ceftriaxone (CRO), levolfoxacin (LVFX) and azithromycin (AZM) were individually detected. Result All of 30 strains of Haemophilus inlfuenzae formed various BF. After BF is formed, the increase of MBEC for different antibiotics was inconsistent with the increase of MIC and MBC. The difference was statistically signiifcant (MBEC/MBC, H=91.54;MBEC/MIC, H=87.91;all P<0.001). The MBEC of AMP was the highest, up to 100 times than the MBC and MIC. The MBEC of CRO was dozens of times than the MBC and MIC. The MBEC of LVFX and AZM were most close to those of MBC and MIC. Conclusion After the formation of BF, resistance to antibiotics of Haemophilus inlfuenzae is enhaced. LVFX and AZM showed more favorable effect on Haemophilus infuenzae BF.
8.Analysis on contrast application of different instruments in open thyroid operation
Yunchao XIN ; Xiaoling SHANG ; Gang XUE ; Yantao LIN ; Guogang XU ; Qingjun ZHANG
Chongqing Medicine 2015;(15):2057-2059
Objective To compare the clinical therapeutic effect of LigaSure vessel sealing system ,ultrasonic scalpel and mo‐nopolar high frequency electrocautery in open thyroid operation .Methods 293 cases of thyroid operation sequentially admitted in our hospital and in accordance with the inclusion criteria were collected and divided into three groups by the method of random dig ‐its table .There were 98 cases in the LigaSure vessel sealing system group (L‐Sure group) ,100 cases in the ultrasonic scalpel (US group) and 95 cases in the monopolar high frequency electrocautery (M‐E group) .The intraoperative and postoperative conditions were compared among 3 groups .Results The operation time ,intraoperative amount of bleeding and postoperative drainage in the L‐S group and the US group were significantly less than those in the M‐E group (P<0 .05) ,moreover the patient′s pain and the hos‐pitalization stay were reduced and the postoperative recovery was promoted (P<0 .05);but the differences between the L‐S group and the US group had no statistical significance (P>0 .05) .Conclusion The LigaSure vessel sealing system and ultrasonic scalpel can shorten the operation time ,reduce the amount of bleeding and postoperative drainage ,relieve pain and reduce the hospitalization duration .
9.Evaluating the left ventricular diastolic function of diabetes mellitus patients using dual-gate Doppler.
Jiani LIU ; Liping CHEN ; Xiaoling ZHANG ; Yanwen SHANG ; Fang WANG ; Hong TANG
Journal of Biomedical Engineering 2015;32(1):163-167
To evaluate the value of left ventricular diastolic function in type 2 diabetes mellitus (DM) using dual-gate Doppler and relative factors, we included 50 non-obesity and hypertension-free DM patients into the controlled group in the study along with 48 age-and-gender-matched healthy volunteer subjects. The peak early diastolic velocity (E), peak later diastolic velocity (A), deceleration time (DT), anterior mitral annulus diastolic peak velocity (e') , isovolumic relaxation time (IVRT), E/A, E/e', Tei index and T(E-e), were measured with dual-gate Doppler. 20 subjects were randomly selected for repetitive analysis. Study showed statistical difference in E/A, DT, e', E/e', IVRT, Tei index and T(E-e), between the two groups (P < 0.05). Linear regression analysis showed positive correlation between T(E-e), and IVRT, course of DM patients and T(E-e), (Beta = 0.295, P = 0.020), and HbA1c control level and T(E-e), (Beta = 0.399, P = 0.010). Repeated analysis showed good reproducibility for both within and between groups. Dual-gate Doppler has clinical value in evaluating left ventricular diastolic function in type 2 diabetes mellitus patients. The course of type 2 diabetes mellitus patients and HbA(1C) control level were both closely related with left ventricular diastolic function.
Case-Control Studies
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Diabetes Mellitus, Type 2
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physiopathology
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Diastole
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Echocardiography, Doppler
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Humans
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Hypertension
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Mitral Valve
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Regression Analysis
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Reproducibility of Results
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Ventricular Dysfunction, Left
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Ventricular Function, Left
10.Effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
Xue GAO ; Yutuo ZHANG ; Yantao LIN ; Haifeng LI ; Yunchao XIN ; Xiaolei ZHANG ; Yunpeng XU ; Xiaoling SHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(10):721-723
OBJECTIVE:
To establish a biofilm model of Haemophilus influenzae and observe the effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
METHOD:
Thirty strains of Haemophilus influenzae were isolated from adenoids of children with adenoidal hypertrophy. Two strains which could build stronger biofilms was selected in a 96-well plate. The effect of ambroxol on biofilms were determined by crystal violet, and the structure of biofilms were observed by scanning electron microscope (SEM). The numbers of viable bacterial in biofilm after ambroxol treatmented determined by plate culture count.
RESULT:
Through crystal violet assay, significant difference (P < 0.01) between the two group after treatment was found when ambroxol concentration reached at 0.25 mg/ml and 0.49 mg/ml. The biofilms was destroyed by SEM. Ambroxol had the positive effect on bacterial killing by plate culture count,and the effect was in a dose dependent.
CONCLUSION
Ambroxol could destroy the biofilm of Haemophilus influenzae, and had bactericidal function in vitro.
Ambroxol
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pharmacology
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Biofilms
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drug effects
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Child
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Child, Preschool
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Haemophilus influenzae
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drug effects
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Humans
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Microbial Sensitivity Tests