OBJECTIVE:To establish a method for the determining contents of 2 lignan components[dehydrodiisoeugenol and 2,3-dihydro-7-methoxy-2-(3,4-methylened ioxyphenyl)-3-methyl-5-(E)-propenyl-benzofuran(referred to“lignanoid 2”)]. METH-ODS:HPLC method was adopted. The column was Elite C18 with the mobile phase of water-methanol(gradient elution)at the flow rate of 1.0 ml/min;the detection wavelength was 225 nm and the column temperature was 30 ℃. The sample size was 20 μl. RE-SULTS:There was a good linear relationship between sample quantity and the peak area in the range of 0.202-2.02 μg(r=0.999 9) and 0.204-2.04 μg(r=0.999 9)for 2 lignan components. The RSD of precision,stability and repeatability tests were less than 2%with the average recovery of 101.54%(RSD=0.60%,n=6)and 99.43%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,sensitive and accurate,and can be used for the quantization determination of dehydrodiisoeugenol and lignanoid 2 in nut-meg-5.

Objective To evaluate the behavior and bone destruction of the mouse model of bone cancer pain signs. Method A mouse model of bone cancer pain signs was developed by intra-femur inoculations of Lewis lung carcinoma cells in C57BL/6 mice. Spontane-ous lifting time, ambulatory score and paw withdrawal latencies to radiant heat stimulation were measured in alternative days throughout the experiment. The structural damage of the femur were monitored by radiogram on the 7th, 15th and 23rd day respectively, and the pathohisto-logical changes of the femur bones were observed by hematoxylin-eosin staining (HE) staining on the same days. Meanwhile, the glial fibril-lary acid protein (GFAP) immunohistochemistry changes of the spinal cord in lumbar segments on the 23rd day after inoculation were ob-served. Results Mice received intra-femur inoculation of Lewis lung carcinoma cells gradually developed spontaneous pain, which was be-ginning on the 11th day after inoculation, followed by move-evoked pain and thermal allodynia. On the 23rd day after inoculation, X-ray film showed that medullary cavity of ipsilateral distal femur were filled with tumor cells and full thickness cortical bone was lost. Furthermore, tumor cells invaded peripheral muscles. Astrocytes on the inoculated side of the spinal cord were activated. Conclusion Lewis lung carci-noma cells were a good choice to build a mouse bone cancer pain.

Objective To investigate the effect of immediately lymphatic reconstruction (LR) after heterotopic small bowel transplantation on pharmacokinetics (absorption and transportation) of FK506. Methods Male Brown-Norway (BN) and Lewis (LEW) rats were divided to two groups. Group A: BN→LEW+LR+FK506 ( n =12); Group B: BN→LEW+FK506 ( n =12). FK506 at a dose of 5?mg/kg per day was taken through stoma. At 1, 4, 7 and 14 postoperative day (POD), venous blood 0.5?ml was obtained on the 1.2 , 2 and 3?h after administration of FK506. Whole blood concentration of FK506 was analyzed by microparticle enzyme immunoassay and a kit of monoclone antibody. Results At the every phase of early period after transplantation (1, 4, 7 POD), the levels of FK506 in plasma were significantly higher in group A than in group B ( P

Objective To study the difference between central and peripheral position of contrast gray-scale imaging in rabbit′s liver.Methods Ultrasound contrast agent(UCA) SonoVue TM ( 0.1 ml/kg) was applied respectively in 8 normal rabbits by intravenous bolus injection.Corresponding parameters of the time-intensity curve: time to enhancement(ET),time to peak intensity(PIT),peak signal intensity(PSI),mean transit time(MTT) and area under the curve(AUC) were measured using low mechanical index contrast gray-scale imaging and Wash-in/Wash-out time intensity curve software.Results There was significant difference of parameters of the time-intensity curve between central position and peripheral position of parenchyma ( P

Objective To study the relationship between doses of ultrasound contrast agent(UCA) and contrast-enhancement effects of gray-scale imaging in rabbit liver. Methods Ultrasound contrast agent SonoVue TM in eight different doses ( 0.02 - 0.16 ml/kg) was applied respectively in five normal rabbits in randomized order as a bolus in a marginal ear vein followed by a 1.5 ml saline flush. Corresponding parameters of the time-intensity curve: time to enhancement(ET),time to peak intensity(PIT),peak signal intensity(PSI),mean transit time(MTT) and area under the curve(AUC) were measured using low mechanical index contrast gray-scale imaging and Wash-in/Wash-out time intensity curve software package. Results Along with an increase of the SonoVue TM dose,the MTT and the AUC increased exponentially,and the ET decreased exponentially. The PSI of portal vein and liver parenchyma increased only in the dose range from 0.02 ml/kg to 0.10 ml/kg ( r = 0.89 and 0.92 ,respectively),and decreased with a further increase of the UCA dose. A possible explanation for this effect is the bubble saturation and attenuation. No significant correlation was found between the UCA dose and the PIT( r = -0.35 and 0.22 ,respectively). Conclusions The parameters of time-intensity curve are influenced intensely by different UCA dose. It is important to grasp the relationship between the UCA doses and contrast-enhancement effects for microcirculatory quantification. The 0.10 ml/kg appears to be the optimal dose for tissue perfusion studying because it significantly improved videointensity and duration of enhancement and did not result in the bubble saturation and attenuation.

Objective To investigate the expression of MnSOD and E-cadhefin in nasopharyngeal carcinoma(NPC) tissue and its relationship with clinicopathological features and prognosis. Methods The expression of MnSOD and E-cadherin were detected by immunohistochemistry method in 60 NPC patients. Results Of the whole group,lymph node positive group and lymph node negative group,the strong positive rate of MnSOD protein was 47% (28/60) ,49% (25/51 patients) and 33% (3/9) (x2 =0.76,P =0.382), respectively.The corresponding strong positive rate of E-cadherin protein was 47% (28/60) ,43% (22/51) and 78% (7/9) (x2 =3.69,P =0.047) ,respectively.The expression of MnSOD increased with T stage and N stage.The higher expression of MnSOD was significantly associated with the larger size of metastatic lymph node(r =0.46 ,P =0.002) ,more radioresistance and poorer prognosis,but not with the region of lymph node metastasis(r =0.223,P = 0.116).The lower expression of E-cadherin was closely relevant with higher N stage and the smaller region of lymph node metastasis(r =-0.33,P = 0.020),but not with T stage,lymph node size or radiosensitivity(r =-2.19,P=0.093;r=-0.07,P=0.623;r=-0.18,P=0.170).Multi variate analysis showed that MnSOD and E-canherin were independent prognostic factors (x2= 4.45,P = 0.035;x2 =5.12,P=0.024). Conclusions High expression of MnSOD may stimulate tumor growth and reduce radiosensitivity.High expression of E-cadherin may inhibit lymphatic metastasis,while has no rela tionship with tumor growth and invasion.MnSOD and E-cadherin could affect the prognosis of NPC patients.

Objective To analyze the clinical manifestations and the features of brain MRI and cerebrospinal fluid (CSF) findings in adult Chinese patients with anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis.Methods We reviewed the clinical manifestations,brain MRI and CSF examinations of 29 patients who were diagnosed as anti-NMDAR encephalitis.Results The major clinical features of anti-NMDAR encephalitis patients included psychiatric symptoms (86％,25/29),seizures (83％,24/29),decreased consciousness (55％,16/29),involuntary movements (55％,16/29),central hypoventilation (34％,10/29),and hypersalivation (17％,5/29).Some patients also experienced autonomic instability,hemiplegia and aphasia.Underlying ovarian teratoma was identified in 14％ of affected patients(4/29).Brain MRI was found abnormal in up to 62％ patients (18/29),located in the temporal lobes,hippocampus,thalamus,brain stem,cingulate gyrus,frontal and parietal cortex,corpus callosum,internal capsule,basal ganglia and periventricular area.CSF findings were abnormal in 83％ of patients with anti-NMDAR encephalitis.Oligoclonal banding in CSF was positive in 95％ patients (19/20).The recurrence rate during 3 years was 31％ (9/29).Conclusions Anti-NMDAR encephalitis is a treatable disease,yet with high recurrence rate.Its predominant clinical features are psychiatric symptoms and seizures,while involuntary movements,central hypoventilation and hypersalivation are its characteristic manifestations.Lesions in MRI are widespread,not only restricted to limbic lobe.