Objective To study the expression of P450arom(P450A)protein and mRNA in human ovarian endometriosis and normal endometrium and the relationship with its local estrogen abnormal synthesis.Methods PT-PCR was used to assess P450arom messenger RNA(mRNA)expression of 45 ovarian endometriosis and 35 normal eutopic endometrium;immunohistochemical assay was performed to locate and examin the protein expression of P450arom in the above cases.Results P450A mRNA level was higher in the ectopic endometrium than that in eutopic endometrium(P

OBJECTIVE: To establish RP-HPLC method for the content determination of chlorogenic acid in 3 kinds of dosage forms of Qingkailing preparation. METHODS: SinoChrom ODS-AP C18(250 mm?4.6 mm, 5 ?m) column was adopted and the mobile phase consisted of methnol-water-acetic acid (25 ∶ 75 ∶ 0.5) at the flow rate of 1.0 mL?min-1. Column temperature was set at 30 ℃ and the detection wavelength was set at 327 nm. RESULTS: The linear range of chlorogenic acid was 3.81～152.50 ?g?mL-1(r=0.999 8), and the average recoveries of the capsules, granules and dripping pills were 99.03%(RSD=2.02%), 99.35%(RSD=1.16%),98.11%(RSD=1.51%). CONCLUSION: The method is sensitive, simple and accurate for the quality control and content determination of 3 kinds of Qingkailing preparations.

Objective To explore the effect of recombinant bovine basic fibroblast growth factor(rb-bFGF)combined with boric acid solution on vulval ulcer.Methods 64 patients with vulval ulcer were divided into two groupa randomly.33 patients( group A) with vulval ulcer was treated with rb-bFGF and boric acid solution locally,while the others (group B) accepted ultra-red rays.Before the different treatment above,metronidazole or clotrimasole was put into the vaginae of all the patients,which were given relative medicine according to etiopathogenesis.The two groups were treated continually for two weeks and then were examined.Results The effective rate in group A was significantly higher than that in group B,which was the same result as the ulcer heating time between two groups.Conclusion rb-bGFG combined with boric acid solution is an effective remedy for the treatment of vulval ulcer.

Objective To analyze the efficacy and safety of combination of rh-endostatin (Endostar) with docetaxel treatment on patients of non-small cell lung cancer (NSCLC) who presented progressive disease (PD) after first-1ine chemotherapy.Methods From Janury 2010 to March 2013,76 patients with stage Ⅲ B/ Ⅳof NSCLC experienced previous chemotherapy of one-regimen were screened for this trial.Given docetaxel combined with recombinant human endostatin (combined group, 38 cases) and docetaxel (chemotherapy group, 38 cases) to continue treatment.The response, time to progression(TTP) and adverse effects were observed in both groups.Results The objective response rate (ORR) and clinical benefit rate (CBR) were 0 and 63.2％ (24/38) in the combined group, along with 0 and 52.6％ (20/38) in the single docetaxel group, with a nonsignificant difference between the two groups (P =0.712).The median TTPs in the combined and single docetaxel groups were (2.6±0.4) months and (2.0±0.8) months respectively (P =0.083).The median TTPs of the patients with SD after therapeutic cycles in the combined and single docetaxel groups were (6.2±0.4) months and (3.2 ± 0.8) months respectively (P =0.038).The differences between two groups were nonsignificant in adverse, serious adverse and cardiovascular adverse effects(P＞0.05).Conclusion Endostar may prolong TTP in patients with advanced NSCLC benefited from docetaxel treatment without increased toxicities.

Objective To study the relationship of apoptosis-related gene Survivin with Bcl-2,Bax protein in adenomyosis.Methods The expressions of Survivin,Bcl-2 and Bax protein were deteeted by immunohistochemical method(SP)in ectopic and eutopic endometrium of adenomyosis from 46 cases,and compared with that in eutopic endometrium from endometrium 30 cases without adenomyosis.Results The expression rate of Survivin protein in ectopic endometrium of adenomyosis was much higher than that in normal endometrial cells.The Survivin was positively corelated to Bcl-2,negatively to Bax.Conclusion Survivin may play an important role in pathogenesis of adenomyosis.With Survivin gene,apoptosis-related gene Bcl-2 may have a synergic role and Bax have an antagonistic role in the formation and progression of adenomyosis.

OBJECTIVE: To evaluate the improvement of speech perception in Chinese-native cochlear implant (CI) children using frequency modulated system (FM system). METHOD: The mandarin speech perception (MSP) system was used to evaluate 11 cases with severe and profound hearing loss who were fitted cochlear implants. Listeners were asked to repeat MSP words presented in quiet and several different signal-to-noise ratio (SNR) conditions and percent correct word repetition was determined. Performance was evaluated under FM system and without FM (CI only). In addition, the listeners' subjective performance changes in the experiment were also observed. RESULT: (1) There was significant main effect of the device condition (with FM and no-FM) (F = 72.938, P < 0.01), a significant main effect of signal level (F = 230.715, P < 0.01), a significant interaction effect between the signal level and the device condition (F = 40.893, P < 0.01). (2) Listeners answered the question in a louder voice, showed more confidence, when using with FM system. CONCLUSION FM system could improve the speech reception in a complex environment for Chinese-native CI children.
Child ; Cochlear Implantation ; Cochlear Implants ; Humans ; Noise ; Signal-To-Noise Ratio ; Speech ; Speech Perception ; Speech Therapy

Objective To study the influence of different bed rest time on orthostatic hypotension,and explore a feasible and appropriate bed rest time for elderly patients after hemodialysis.Methods 58 elderly patients with regular hemodialysis were selected from January 2011 to May 2012.By self-control,they were divided into three groups according to different bed rest time:T0 group who got up immediately after the hemodialysis,T10 group who got up after 10-minute bed rest,T20 group who got up after 20-minute bed rest.The incidence of orthostatic hypotension was observed and compared between three groups.Results Compared to T0 group,the incidence of orthostatic hypotension in T10 and T20 groups was significantly reduced.However,no difference was seen between T10 and T20 groups.The acceptability rate in T10 group was 67.24％,higher than 17.24％ of the T20 group.Conclusions Elderly hemodialysis patients should continue to lie down for at least 10 minutes after hemodialysis,and then slowly get up,this measure can reduce the occurrence of orthostatic hypotension.

Objective To observe the effect of lung suctioning method on mechanical ventilation related pulmonary infection of patients after cardiac valve replacement. Methods 102 patients of mechanical ventilation related pulmonary infection after cardiac valve replacement were randomly divided into the treatment group( 50 patients )and the control group( 52 patients ). The treatment group received intervention of lung suctioning method and the control group adopted routine sputum aspiration. The pulmonary infection rate and blood gas analysis before and after the treatment were observed and the cure rate and the positive rate of specimen of sputum were recorded. Results The pulmonary infection rate of the treatment group was lower than the control group;PaO2 and SaO2 were obviously different after treatment in the control group, but no difference in the treatment group. The cure rate of pulmonary infection patients in the treatment group was higher than those in the control group. Conclusions The intervention of lung suctioning method was a safe and effective method to improve hypoxemia and abate the rate of pulmonary infection.

By means of literature review of existing laws and regulations,an analysis was made on main setbacks found in the clinical trials and regulation of medical devices in China.The authors proposed to enact and improve such laws and regulations as soon as possible.

Objective To investigate the effects of Astragalus Polysaccharide (for intravenous injection) in stimulating the secretion of hematopoietic growth factors from activated human peripheral blood mononuclear cells (PBMC). Methods Human PBMC were isolated from concentrated leukocytes and were cultured with Astragalus Polysaccharide. Hematopoietic growth factors such as granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA). Results Astragalus Polysaccharide stimulated G-CSF and GM-CSF formation in a dose-dependent and a dose-effect and time-dependent manner. Conclusion Astragalus Polysaccharide can stimulate the secretion of hematopoietic growth factors from activated human PBMC, indicating its clinical application for leukocytopoiesis.