Objective To investigate the clinical effect of enzyme linked immunosorbent assay (ELISA) in detecting hepatitis C antibody. Methods 200 cases of patients with hepatitis C was measured by ELISA, the gold standard method for detection of hepatitis C virus antibody, recording the test results, the data input SPSS software to give the corresponding analysis and conclusions. Results 200 cases of patients with hepatitis C were successfully completed the gold standard method and ELISA method for detection of hepatitis C virus antibody, the analysis showed that positive rate of ELISA method for detection of hepatitis C virus antibody was significantly higher than that of the gold standard method, the positive rate of ELISA was 82.50％, the positive rate of the gold standard method for was 50.00％, results suggested that HCV detection rate of ELISA method is significantly better (P<0.05). Conclusion The method of ELISA can be used for the detection of hepatitis C antibody, which has positive significance in improving the diagnostic accuracy of hepatitis C, and is beneficial to protect the efficacy and quality of life of patients.

OBJECTIVE:To provide reference for standardization of drug package.METHODS:5 dose-related items stated in a total of 426 package inserts of chemical drugs collected from the drug storeroom in our hospital were investigated and analyzed.RESULTS:Only 32.39% of the package inserts were with complete labeling.The labeling rate for special group was low and only 40% labeled the maximal dose.Less than 2% of child dose stated the doses according to body surface area,and the problem of over-dose was common.Only 27.46% had the item of "under supervision of a physician".CONCLUSION:It is necessary to strengthen supervision on drug package inserts and improve cognition on the importance of drug package inserts.

Objective To study the CT and MRI features of cystic brain metastases. Methods 26 cases of cystic brain metastases proved by clinicopathology were presented.There were13 males and 13 females with an average age of 52.7 years.CT studies were obtained in 15 cases,while MR studies were done in 13 cases.11 cases had CT and/or MRI contrast enhancement studies. Results According to the shape of the wall of cystic brain metastases,48 tumors in 26 cases were classified into three types: irregular,nodular,regular type.The wall demonstrated iso-or slightly high density on CT and iso-or slightly low intensity on MR T_1WI and T_2WI,while the hydatid fluid showed slightly high intensity on MR T_1WI and high intensity on T_2WI and iso-or slightly high density on CT compared to CSF.The tumors showed circular reinforcement on enhanced CT or MRI,with nodular enhancement in some cases. Conclusion Cystic brain metastases have mutiple types and should be differentiated from brain abcess and other cystic brain parenchamal tumors.Multi-direction enhanced MRI is helpful to diagnosis.

Objective To observe the effect of continuity self -management education on the emotional state and quality of life in patients with peritoneal dialysis and provide guidance for health intervention.Methods 236 patients with peritoneal dialysis were selected and randomly divided into two groups.The control group(118 cases)was just given routine nursing care,the continuity self -management education group(118 cases)was given continuity self -management education and routine nursing care.After 4 months,the differences of anxiety,depression and quality of life between the two groups were evaluated.Results Four months after the intervention,anxiety and depression scores of the two groups became lower,which of the continuity self -management education group decreased more significantly than the control group(anxiety:t =9.860,P =0.000;depression:t =15.265,P =0.000).The quality of life scores of the two groups became higher,which of the continuity self -management education group were better than the control group (somatization:t =26.767,P =0.000;force:t =8.090,P =0.000;sensitive interpersonal relationship:t =2.414,P =0.017;depression:t =67.484,P =0.000;anxiety:t =8.198,P =0.000;hostile:t =26.366,P =0.000;paranoid:t =4.862,P =0.000;Psychotic:t =0.951,P =0.343).Conclusion Continuity self -management educa-tion has a positive effect on emotional states and quality of life,which is worthy of clinical promotion.

Since the tumor has become one of the most deadly diseases to human life ,it is of great impor-tance to explore a new effective antitumor medicine .This paper summarizes the chemical constituents and the mechanism of Inonotus obliquus .The mechanism includes the details of the inhibition of tumor cell proliferation , inducing tumor cell apoptosis ,influence tumor cell cycle etc .Meanwhile,it describes Inonotus obliquus to the ad-juvant chemotherapy of tumor .

Objective To explore the effect of recombinant bovine basic fibroblast growth factor(rb-bFGF)combined with boric acid solution on vulval ulcer.Methods 64 patients with vulval ulcer were divided into two groupa randomly.33 patients( group A) with vulval ulcer was treated with rb-bFGF and boric acid solution locally,while the others (group B) accepted ultra-red rays.Before the different treatment above,metronidazole or clotrimasole was put into the vaginae of all the patients,which were given relative medicine according to etiopathogenesis.The two groups were treated continually for two weeks and then were examined.Results The effective rate in group A was significantly higher than that in group B,which was the same result as the ulcer heating time between two groups.Conclusion rb-bGFG combined with boric acid solution is an effective remedy for the treatment of vulval ulcer.

Objective This study was designed to investigate the relationship between COX-2 and VEGF-C in human gastric carcinomas and discuss the significance of COX-2、VEGF-C in lymph mode metastasis.Methods Forty-two gastric carcinoma tissues and twenty adjacent noncancerous specimens were obtained from surgical resections.COX-2,VEGF-C protein expression was evaluated by immunohistochemical analysis.Results (1)COX-2 was overexpressed in 25 of 42 carcinoma tissues (59.5%) and VEGF-C was 27 of 42(64.3%),which were significantly higher than those in noncancerous tissues (P0.05).(3)There was positive correlation between the expression of COX-2 and VEGF-C(r=0.425,P

Objective To study the different expression of complement receptors on neutrophils between bacterial and viral pneumonia. Methods Fifty-nine patients with CAP were studied prospectively. CR1 ,CR3 were measured by,using flow cytometer. ESR,CRP and WBC were also measured. Results Thirteen patients had pneumococcal pneumonia; 13 patients, A type of infuenza virus pneumonia ;5 patients, atypical pneumonia, and 28 patients, aetiologically undefined pneumonia. The mean expression of complement receptor 1 ( CR1 ) on neutrophils and aetiologically undefined pneumonia was( 19.4 ± 9.8 ) and ( 19.3 ± 8.8), which were significantly higher than those with A type of infuenza virus( t = 6.13,5.09 ,P < 0.01 ). Conclusion The expression of CR1 may be of value as an additional rapid tool in the aetiological diagnosis, bacterial or viral infection of CAP.

Antimutagenic effect of p-aminobenzoic acid (PABA) and sodium bisulfite (NaHSO_3)on the mutagenicity of N-methyl-N-nitro-N-nitrosoguanidine (MNNG) was detected by the mehod of human lymphocytes unscheduled DNA Sythesis (LIDS). The results showed that when the concentration of PABA and NaHSO_3 was varied in presence of a constant concentration of MNNG, 5 ?10~(-5) mol/L concentration of PABA, 3 ? 10~(-4) mol/L concentration of NaHSO_3 were the most effective, while the concentration of MNNG varied in the presence of a constant concentration of PABA and NaHSO_3, antimutagenicity was the most effective at high concentration of MNNG. The results indicated that the PABA and NaHSO_3 exhibited antimutagenic activity towards MNNG-induced mutagenicity in LIDS and the extents of antimutagenicity were related with the concentration of MNNG.

Objective To study the relationship between the mutation of androgen receptor(AR) gene and the development of human prostat e cancer. Methods All exons of AR gene except trineucl eic acid repeat polymorphism were amplified by PCR and selected by single strand conformation polymorphism( SSCP) in 27 paraffin-embedded slides of human pros tate cancer. The abnormal mobility shifts were further detected by direct DNA c ycle sequencing. Results Three abnormal mobility shifts respectively in exon A.D.G were found by SSCP in three prostate cance r tissues. One point mutation was found in 296 amino acid (C 966→A,Ser29 6Arg) from exon A.This point mutation has not been reported before. Conclusions This mutation might change the transcriptive activity of AR, and it might be correlated to PCa progression.