Objective To research on the proliferation and differentiation in vitro of primordial germ cells. Methods We got plenty of primordial germ cells(PGCs) by co-culture of PGCs with sertoli cells(SCs).The primary cells formed embryoid bodies(EBs) by suspension culture.furthermore,these EBs were plated on gelatin-coated culture plates for spontaneous differentiation. Results We got plenty of PGCs by coculture.Primary PGCs and passaged PGCs for histochemistry staining with AKP and immunohistochemistry staining for SSEA-1 display positive character.The EBs by suspension culture differentiated into epithelial-like,fibroblast-like and neuronal-like cells in vitro. Conclusion Primordial germ cells can proliferate and keep the character of stem cells by co-culture with SCs.PGCs can form EBs by suspension culture,most of these EBs can differentiate into neuronal-like cells,they also can differentiate into epithelial-like and fibroblast-like cells in vitro.

Objective To investigate the therapeutic efficacy of the canalith repositioning maneuver to benign paroxysmal positional vertigo (BPPV). Methods Epley maneuver, Barbecue rotation and Semont maneuver were applied to twelve cases of BPPV. Results Positional vertigo in all subjects disappeared completely 48 hours later by treatment with manipulative reduction, and no obvious adverse reaction was found. There was no recurrence during a follow up from 3 to 11 months.Conclusion The canalith repositioning maneuver is effective, simple and safe for the patients with BPPV and may be recommended as the first-selected treatment modality.

Objective To study the relationship between hyperhomocysteic acid(HHcy) and artherosclerosis.Methods 20 male rabbits were randomly divided into control and experiment groups(n=10).HHcy models were made by hypodermical injection of DL-methionine for 7 weeks.After 7 weeks homocysteic acid(Hcy),IL-1?,IL-6,and IL-8 in serum of rabbits in two groups were measured by enzyme linked immunosorbent assay(ELISA).And the pathomorphological changes of aorta were observed with HE staining.The number of NF-?B positive cells in the aorta were counted by immunohistochemical method.Results The levels of Hcy,IL-1?,IL-6 and IL-8 in experiment group were higher than those in control group(P

Objective To describe the disease's spectrum of fever patients,especially to analyze the epidemic characterization of infectious diseases,to offer the proof for fever department in screening of infectious diseases.Methods A total of 15081 patients were analyed for patients presenting to the Infectious Diseases Department,People's Hospital,Peking University,between June 2005 to May 2006.A retrospective study was used to analyze the disease's spectrum and epidemic characterization.Results Among the disease's spectrum of fever patients,the first leading cause was respiratory diseases(78.22%),followed by infection with the special patients(10.50%)、acute gastroenteritis(3.57%)、the infection at other site with etiological factor determined(2.82%)、infectious diseases(2.30%)、urinary system infection(2.06%) and fever of unknown origin(0.53%),respectively;Among the spectrum of infectious diseases,the first leading cause was infectious diarrheal disease(29.97%),followed by measles(24.78%)、lung tuberculosis(11.24%).Conclusion The spectrum of fever is complex.To obtain the epidemic characterization of infectious diseases,it's helpful to the fever department to screen and prevent the infectious diseases.

Purpose:Home-made NiTi-stent was used in the study to observe the biological compatibility.Materials card Methods:Stents were implanted within the bile ducts,arteries,veins,bronchi of swine by surgery.Swine was killed according to the tie schedule to observe the patency of stents.Results:6 of 11 biliary stents were occluded completely,with only 5 stents partially patent.The degree of occlusion was related to the time period.10 of 11 femoral stents were patent,only 1 stent was occluded completely.1 of the only 1 venous stent was occluded completely,4 of 8 bronchial stants were patent.Epithlium tissues were found along the surfaces of stents.Both sides of the stents were covered by the epithelium cells partially and dominated by the proliferation of connective tissues and parenchymal cells of the organs.The constitution of proliferated tissues was related to the implanted time period of the stents.No connective tissues were found within two weeks,but obvious proliferation of connective tissues were found associated with lympheytic tissues.Conclusion:The dagree of surface covering of the home- made stent by epithelial the rate of was related to the diameters of the of and the lumceh stent implantation segment also the flow volume within the stent,and the period of stent implantation.Good results can be yielded by selecting the suitable stent and the implanting site.

Analyse ethic problem in hospital infection management process through discussing cause and effect of Infection and put forward to resolve approach and countermeasure.

Objective To measure and compare sevoflurane minimum alveolar concentration (MAC)in newborn and adult rats.Methods The rats were divided into newborn rat (6-8 days) group (25 cases)and adult rat (8-10 weeks)group (25 cases).All rats were settled in the self-made device for anesthesia with inhaled sevoflurane,and the tails of rats were exposed out of the device. Sevoflurane was given via an anesthesia machine.Up-and-down method and clamping tail stimulus were applied to measure the MAC values of sevoflurane in the two groups.1.5% of sevoflurane was set as the initial concentration,and± 0.2% as adjustable gradient.A positive or negative response was judged by clamping tail stimulus in a 20 minutes interval.The mean MAC value of all rats in each group was defined as the MAC value.Results The MAC value of newborn rats was (2.58 ± 0.1 1)%,and the MAC value of adult rats was (2.32 ±0.13)%.A significant difference was found between the two groups (P <0.01).Conclusion The MAC value of newborn rats was much higher than that of adult rats.Newborn rats need a higher concentration of sevoflurane at the same depth of anesthesia when compared with adult rats.

The patients with cor pulmonale in release stage have been treated and cared with many methods.The results showed that there are obvious recuperatio of respiratory symptoms and lung function and evident strength of the immune function of the body and movement of diaphragm in the patients of treatment group with cor pulmonale compared with control group.The studies suggested that the rehabilitation treatment and care plays an important role in treating patients with cor pulmonale,particularly postponing and prevention acute attack.

Objectives To observe the pre and post-operational changes of the expressions of survivin, caspase-3 and CD44V6 in patients with colorectal cancer after intra-abdominal implantation of sustained releasing fluorouracil. Meth-ods Sixty-four patients with colorectal cancer (Dukes’stage of B and C) were divided into treatment group and control group, 32 patients in each group. The standard radical surgery was performed in two groups of patients. The fluorouracil im-plants were implanted intra-abdominally in treatment group. The peripheral blood levels of surviving and caspase-3 were de-tected by RT-PCR. The level of CD44V6 was detected by flow cytometry in two groups of patients. Results There were no significant differences in levels of survivin, caspase-3 and CD44V6 before surgery between two groups (P>0.05). The level of survivin (0.362 ± 0.183) was significantly lower at 14 days after operation in treatment group than that of control group (0.585±0.207), but the level of caspase-3 (2.001±0.146) was significantly higher than that of control group (1.654±0.111). The levels of CD44V6 were significantly lower in treatment group (1.857±0.535) and control group (3.471±0.496) after opera-tion than those before operation (9.557±1.170 and 9.729±0.943, P＜0.05), and the level of CD44V6 was significantly lower in treatment group than that of control group (P＜0.05). Conclusion The implant for the sustained release of fluorouracil showed a positive impact on micrometastases and prognosis of colorectal cancer, while improved the long-term efficacy of postoperative colorectal cancer.

Objective To evaluate the inhibitory effect of butyl flufenamate (BT) on ultraviolet (UV)-induced acute skin phototoxic reaction,and to investigate its possible mechanisms.Methods Eight SKH-1 hairless mice were included in this study.The back of each SKH-1 hairless mouse was divided into six regions,which were then randomly classified into six groups:blank group receiving no treatment,UV group receiving UV radiation only,BT + UV group and vehicle + UV group topically treated with BT ointment and vehicle respectively followed by UV radiation,UV + BT group and UV + vehicle group topically treated with BT ointment and vehicle respectively after UV radiation.Skin samples were obtained from these mice at 24 hours after treatment.Subsequently,hematoxylin-eosin (HE) staining was performed,real-time PCR was carried out to detect mRNA expressions of caspase-3,p53,COX-2,PGER1,interleukin (IL)-1β,IL-6,and an immunofluorescence assay was conducted to observe the expression of caspase-3.Statistical analysis was carried out by repeated-measures analysis of variance (ANOVA).Results Compared with the UV group,both BT + UV group and UV + BT group showed a decrease in the degree of skin edema and number of apoptotic cells at 24 hours after UV radiation.Real-time PCR showed that the mRNA expressions of caspase-3,p53,COX-2,PGER1,IL-l β and IL-6 were significantly higher in the UV group than in the blank group (all P ＜ 0.05),but significantly lower in the BT + UV group than in the UV group (all P ＜ 0.05),and only the expressions of caspase-3 and p53 mRNAs were significantly decreased in the UV + BT group compared with the UV group (both P ＜ 0.05).The immunofluorescence assay revealed that the expression of caspase-3 increased in the UV group compared with the blank group,but decreased in both BT + UV group and UV + BT group compared with the UV group.Conclusion BT could partially inhibit UV-induced acute skin phototoxicity in SKH-1 hairless mice.