1.Advance in DNA tumor vaccine
Cancer Research and Clinic 2010;22(3):214-216
DNA vaccine is one of the hot spot in the research of vaccine now. DNA tumor vaccines mainly include tumor-associated-antigens-based completed, epitope, idiotope determinants DNA vaccine,fusion DNA vaccines, RNA self-replicating vaccines, dendritic cell-based tumor vaccines etc. The recent developments are discussed.
2.Fatigue Self-assessment Scale(FSAS)
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
Fatigue Self-assessment Scale(FSAS)was developed on the basis of review on the study of fatigue assessment in both China and overseas countries and considering the Chinese culture background.The scale consists of 23 items and is used to assess the type and severity of fatigue(including three subscales of measuring physical fatigue,mental fatigue and the consequences of fatigue) and characteristics of fatigue(including three subscales of measuring responsiveness of fatigue to sleep/rest,the situation specifi city of fatigue and time mode of fatigue) among the people of sub-health and various diseases with fatigue.It may also be used to assess intervention effectiveness of fatigue.
3.Clinical characteristics of congenital long QT syndrome families
Xiaolin XUE ; Jiangfang LIAN ; Changcong CUI
Journal of Xi'an Jiaotong University(Medical Sciences) 2003;0(06):-
Objective To elucidate the clinical manifestati on s and electrocardiogram characteristics of congenital long QT syndrome families and try to find out the genotype of the long QT syndrome(LQTS) patients. Methods The routine clinical check up and ECG recordings we re done for the 3 family members. Both QT interval and QTc were measured. Diagno stic criteria for LQTS were defined by Schwartz. Results Fifteen family members were identified as with LQTS and 11 members with intermediate probability to LQTS. The clinical manifestatio ns and ECG characteristics were different from each other. Conclusion The clinical manifestations and ECG characterist ics of LQTS patients from family 1,family 2 and family 3 correspond with LQT2, L QT1 and LQT3, which is caused by HERG,KVLQT1 and SCN5A gene mutation.
4.Purification of monoclonal antibody against PGRP and its activity for small cell lung cancer in vitro
Xiaolin ZHOU ; Zhenwei XUE ; Zengli LIU ; Qiaoling XU ; Meiping CUI
Cancer Research and Clinic 2011;23(7):467-470
Objective To explore the effect of purification on monoclonal antibody (MAb) against PGRP by Protein A-Sepharose affinity chromatography, and to provide some based data for the purification of other antibody using the same method. Methods The ascites which include MAb was purified by Protein A-Sepharose affinity chromatography. The purity and activity of MAb was tested by SDS-PAGE and ELISA. The biological function was identified by flow cytometer and immunohistochemistry. Results The average concentration of protein in ascites before purification is 23.62 mg/ml. Before and after purification, the total protein is 148.79 mg and 146.67 mg, respectively. The recovery coefficient of protein is 98.58%. The concentration of MAb in ascites is 5.21 mg/ml averagely. The MAb purity is more than 95 %. The immunoactivity of purified antibody is higher than that of unpurified antibody. Conclusion The purity of MAb against PGRP purified by Protein A-Sepharose affinity chromatography is very high. The immunoactivity of purified antibody is higher than that of unpurified antibody. So the ProteinA-Sepharose affinity chromatography is a rapid, convenient and reliable method for the purification of MAb Against PGRP.
5.Clinical characteristics of 5 Chinese LQTS families and phenotype-genotype correlation.
Jiangfang, LIAN ; Changcong, CUI ; Xiaolin, XUE ; Chen, HUANG ; Hanbin, CUI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(3):208-11
In order to assess the clinical manifestations and electrocardiogram (ECG) characteristics of Chinese long QT syndrome (LQTS) patients and describe the phenotype-genotype correlation, the subjects from 5 congenital LQTS families underwent clinical detailed examination including resting body surface ECG. QT interval and transmural dispersion of repolarization (TDR) were manually measured. Five families were genotyped by linkage analysis (polymerase chain reacting-short tandem repeat, PCR-STR). The phenotype-genotype correlation was analyzed. Four families were LQT2, 1 family was LQT3. Twenty-eight gene carriers were (14 males and 14 females) identified from 5 families. The mean QTc and TDRc were 0.56 +/- 0.04 s (range 0.42 to 0.63) and 0.16 +/- 0.04 s (range 0.09 to 0.24) respectively. 35.7% (10/28) had normal to borderline QTc (< or = 0.460 s). There was significant difference in QTc and TDRc between the patients with symptomatic LQTS and those with asymptomatic LQTS, and there was significant difference in TDRc between the asymptomatic patients and normal people also. A history of cardiac events was present in 50% (14/28), including 9 with syncope, 2 with sudden death (SD) and occurred in the absence of beta-blocker. Three SDs occurred prior to the diagnosis of LQTS and had no ECG record. Two out of 5 SDs (40%) occurred as the first symptom. Typical LQT2 T wave pattern were found in 40% (6/15) of all affected members. The appearing-normal T wave was found in one LQT3 family. Low penetrance of QTc and symptoms resulted in diagnostic challenge. ECG patterns and repolarization parameters may be used to predict the genotype in most families. Genetic test is very important for identification of gene carriers.
Arrhythmia/etiology
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Arrhythmia/genetics
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Asian Continental Ancestry Group
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Electrocardiography
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Genotype
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Long QT Syndrome/complications
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Long QT Syndrome/congenital
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Long QT Syndrome/*genetics
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Pedigree
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*Phenotype
6.Symptom Characteristic of Stagnation of Liver-QI with Deficiency of the Spleen Syndrome and Modern Literature Study on Standards of Syndrome Differentiation
Yuping MA ; Tianfang WANG ; Xiaolin XUE ; Yanling LIU ; Yuehua HE ; Caifeng DU ; Xue LI ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(02):-
Now syndrome study becomes the important research content in our country' TCM field,normalization of the symptoms standardization of syndromes especially becomes scientific research focal point in this field,furthermore some studies exists the conditions of symptoms short of norm and standards of syndrome differentiation discordant,as well stagnation of liver-QI with deficiency of the spleen is clinical common syndrome,so this article carried out all round analysis and investigations on the modern literature of stagnation of liver-QI with deficiency of the spleen syndrome recent ten years,to aim at analyzing the symptom characteristic and standards of syndrome differentiation on stagnation of liver-QI with deficiency of the spleen syndrome,to provide the bases of standardization and normalization of stagnation of liver-QI with deficiency of the spleen syndrome.
7.Connotation of "Fatigue" Symptom and Thought of Its Quantization Evaluation
Xiaolin XUE ; Tianfang WANG ; Yan ZHAO ; Chunguang YU ; Zhigeng LI ; Yuehua HE ; Xue LI ; Caifeng DU ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(05):-
This article analyzed the universality of fatigue existing and the significance of quantization evaluation on fatigue.It expounded the recognition of fatigue in traditional Chinese medicine,and further more proposed the methods of quantization evaluation on fatigue.
8.Comparative study on fatigue characteristics between people with fatigue-predominant sub-health and patients of ulcerative colitis
Xiaolin XUE ; Tianfang WANG ; Jiajia WANG ; Lu YANG ; Guaanru LI ; Yajing ZHANG
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(12):1098-1100
Objective To compare the fatigue characteristics between people with fatigue-predominant sub-health and patients with ulcerative colitis.Methods Fatigue Self-assessment Scale (FSAS) was applied in people with fatigue-predominant sub-health and patients with ulcerative colitis.By using the multivariate statistical analysis,the fatigue characteristics of them were analyzed.Results The fatigue characteristics of two groups of people were different.The scores of physical fatigue,mental fatigue,fatigue consequences,total fatigue,reaction of fatigue after sleep and rest,situation of fatigue in people with fatigue-predominant sub-health were 31.06 ± 17.08,24.63 ± 14.50,24.38±13.39,26.36 ± 11.97,30.63 ± 22.70,42.89 ± 21.47,and in patients with ulcerative colitis were 25.60 ± 22.98,17.80 ± 18.44,20.54 ± 18.53,21.20 ± 17.15,18.18 ± 25.47,41.21 ± 25.70.There were statistical differences on the two groups (P < 0.05 ).There were statistical differences between the percents of four degrees ( unconspicuous,mild,moderate,heavy) of physical fatigue,mental fatigue,fatigue consequences between in people with fatigue-predominant sub-health and in patients with ulcerative colitis (P < 0.05 ).There were statistical differences on the percents of five degrees ( no obvious,slightly,less obvious,obvious,very obvious) of unrelieved fatigue after sleep and rest,situation of fatigue between in people with fatigue-predominant sub-health and in patients with ulcerative colitis (P < 0.05 ).Conclusion The type,severity of fatigue,and characteristics ( such as unrelieved fatigue after sleep and rest,situation of fatigue) are different between people with fatigue-predominant sub-health and patients with ulcerative colitis.
9.Weighting coefficients of symptoms and signs in the diagnosis of corresponding TCM syndrome elements of ulcerative colitis based on expert questionnaire investigation.
Jiajia WANG ; Tianfang WANG ; Xiuyan WU ; Yan ZHAO ; Xiaolin XUE ; Qingguo WANG
Journal of Integrative Medicine 2012;10(4):398-405
To define the weighting coefficients of the symptoms and signs in the diagnosis of corresponding traditional Chinese medicine (TCM) syndrome elements of ulcerative colitis based on expert questionnaire investigation.
10.Characteristics of traditional Chinese medicine syndromes and their element distributions in sub-health status: a modern literature review.
Yajing ZHANG ; Tianfang WANG ; Xiaolin XUE ; Jiajia WANG ; Guanru LI ; Ping HAN
Journal of Integrative Medicine 2008;6(12):1290-3
To probe into the characteristics of traditional Chinese medicine syndromes and their element distributions in sub-health status.