The present study compared effects of sodium selenite(Se), hyaluronidase (Hy) and anisodamine(An) on infarction size(IS), left ventricular myocardial contractility (LVMC) and relaxation(LVMR) on the third day after the ligation of left coronary artery in rats. The results showed that Se, with the dose of 30?g/kg/day either before or after the ligation, has a same effect on reducing IS as Hy and An. However, Se, at the same time, can significantly improve LVMC and LVMR, which were evalued by indexes of LVSP, ?dp/dtmax and T value, while Hy and An can not. It is suggested that Se is superior to Hy and An when they are used in treatment of acute myocardial infarction.

Effects of sex hormones on infarction size(IS) and left ventricular myocardial contractility and relaxation (LVMC, LVMR) in rats after left coronary occlusion in order to make a model of myocardial infarction(MI) were studied. Male adult rats were randomly divided into 3 groups, and pre-treated respectively with testosterone propionate(10mg/kg), oestradioli benzoatis(500?g/kg), or vehicle (0.sml/kg) for 3 weeks (3 times per week) before MI. The injection was continued after MI, on 9th of MI indexes of LVMC and LVMR, IS and the levels of sex hormones were measured. The results showed that: (1) IS were nor affected by both testosterone and oestradiol. (2) Indexes of LVMC and LVMR, i. e. dp/dtmax, LVSP and -dp/dtmax, were lightly inhibited by hyperoestrogencmia(HE), the increasing rate of body weight (IRBW) before and after MI were significantly inhibited by HE. However, indexes of LVMC and LVMR were significantly improved by hypertestosteronemia(HT), but IRBW were not affected by HT. (3) It is interested to note that there was a significant positve correlation between the level of plasma testosterone and indexes of LVMC and LVMR, and an inversive correlation between the level of oestradiol and indexes of LVMC and LVMR, suggesting sex hormones really pay a rolein the pathophysiological process of MI.

Vitamin E( VE) is a major human plasma fat-soluble antioxidant. It has been proved that VE can protect the nervous system,skeletal muscle,retina and the cardiovascular system,etc. Nowadays,more and more researchers focus on the relationship between vitamin E and asthma. This article reviewes the research pro-gresses of the physiochemical properties of VE,and its relationship with asthma.

Objective To study the relationship between hyperhomocysteic acid(HHcy) and artherosclerosis.Methods 20 male rabbits were randomly divided into control and experiment groups(n=10).HHcy models were made by hypodermical injection of DL-methionine for 7 weeks.After 7 weeks homocysteic acid(Hcy),IL-1?,IL-6,and IL-8 in serum of rabbits in two groups were measured by enzyme linked immunosorbent assay(ELISA).And the pathomorphological changes of aorta were observed with HE staining.The number of NF-?B positive cells in the aorta were counted by immunohistochemical method.Results The levels of Hcy,IL-1?,IL-6 and IL-8 in experiment group were higher than those in control group(P

The resection can be performed in about 20 percent of patients with primary hepatocellular carcinoma(PHC) in clinic. However, with the development of interventional treatment such as transcatheter arterial chemoembolization(TACE), better therapeutic effect has been achieved by two-stage resection such as TACE in patients with unresectable PHC. At present, surgical resection is still regarded as the first choice of PHC, and the principle of comprehensive therapeutic solutions around surgical operation has been established. The pretreatment of TACE is an important measure to improve the therapeutic effect in PHC.This paper discussed the mechanism, effect and value of TACE in pretreatment of unresectable PHC. The selection of operative opportunity, sign, and ways after TACE and the factors affecting prognosis of two-stage resection were also reviewed.

Objective To study the effect of the combined medial and lateral operative approach for the serious intra -articular fractures of calcaneus.Methods The lateral and medial approach for the treatment of the serious intra -articular fractures of calcaneus 22 cases (26 feet),fully exposed in the medial and lateral subtalar joint surface,reduct subtalar joint surface,drill into Kirschner nails for reduction of the calcaneus angle,then graft bones fully in the defect left after reduction,used calcaneus anatomic plate and screws to fix the fracture on the lateral. Results The patients were followed up for 10 -22 months.According to the American Orthopaedic Foot and Ankle association ankle scoring system,the averange score was 87 points.Normal union of 22 feet,4 feet delayed union,skin necrosis no incision,no infections,no cases of neurovascular injury.Conclusion The combined medial and lateral operative approach for the serious intra -articular fractures of calcaneus is an effective method,and can fully expose the lateral and medial fracture,and is conducive to reduction of the lateral and medial articular surface,and restoration of calcaneus shape,reduce malunion and the foot -pain with walking.

Objective To investigate the quality of life and its influencing factors of families primary caregivers of dementia patients .Methods The typically self made questionnaire ,WHO quality of life measurement profile (WHOQOL‐BREF) ,zarit bur‐den of care scale(ZBI) ,social support rating scale (SSRS) et al were adopted to investigate 64 caregivers of AD patients .Results (1) The overall quality of life of family caregivers of total subjective feelings were significantly lower than the general health status of subjective feeling score (t= -2 .217 ,P=0 .030) .The field of physiological and psychology points have no statistically significant difference in comparison with Chinese norm (t= -0 .252 ,-1 .994 ,P=0 .801 ,0 .050);the field of social and environment points have statistically significant difference compared with norm(t= -3 .830 ,3 .658 ,P=0 .000 ,0 .001) .(2) caregiver who have spouse , poor parent child relationship and patients with somatic diseases and mental behavior symptoms have lower quality of life(P<0 .05) .(3)Total score of Quality of life was negatively associated with total score of care burden (r= -0 .433 ,P=0 .000) ,while was significant positive associated with total score of the social support (r=0 .346 ,P=0 .005) .(4)Marital status ,parent‐child rela‐tionship ,physical illness and social support were the main factors affecting the quality of life(r2 =0 .409) .Conclusion Strengthen the social support and psychological intervention of dementia caregivers who has a spouse ,poor parent-child relationship and poor physical condition may help to improve the quality of life .

Objective To study the pathogenesis of lower limb motor disorder in vascular parkinsonism(VP)using diffusion tensor image(DTI). Methods A case?control study was performed in a cohort of 16 VP patients and 32 PD patients. Patients were all recruited from the first affiliated hospital of China Medical University from Dec. 2011 to Dec. 2012. The lower limb motor function of those patients was assessed using measurement scales. All patients received magnetic resonance image(MRI)and diffusion tensor image(DTI). Results Patients in VP group showed more seri?ous movement disorders such as freezing of gait(gait ignition failure)than these in PD group,and the movement disorders of their lower limbs prog?ress more rapidly in VP group than the PD group. Movement disorders of their lower limbs in VP group were related to fibers in bilateral frontal lobes (P=0.008). Movement disorder of their lower limbs in PD group were related to fibers in substantia nigra(P=0.030). There are statistically signifi?cant differences in both the FA values of substantia nigra,bilateral frontal lobes and the ADC values of frontal lobes,and the pars compacta and pars reticulata of substantia nigra between these two groups(all P<0.05). Conclusion Movement disorders of bilateral lower limbs are more seriously manifested in VP patients than in PD patients. The onset movement dysfunction of bilateral lower limbs is closely related to fibers in the frontal lobe.

AIM: The present study was designed to examine the effect of Bacillus Calmette-Guerin (BCG) vaccination on blood pressure, nitric oxide (NO) production and iNOS expression in hypertensive rats. METHODS: Renal hypertension (RH) were made by renal artery stenosis in SD rats and the sodium induced hypertensive (SH) rats were made by feeding the rats with high sodium chloride diet (5 g NaCl/100 g food). After four weeks, the hypertensive animals were subjected to the experiment. All the rats were vaccinated with BCG (0.1 mL, i.d) and blood pressure were examined every week. Greiss reaction was used to measure the urinary NO excretion and Western blot was applied to probe the iNOS protein expression in aortic tissue. RESULTS: It was shown that one week after BCG vaccination, the blood pressure decreased significantly in hypertensive rats induced by NaCl-overloading and renal artery stenosis, but not in normotensive control rats. Furthermore, the hypotensive effect of BCG vaccination was enhanced by co-administration of L-arginine. A significant increase in NO production was observed in hypertensive rats. Also, Western blot showed BCG vaccination led to an obvious increase in iNOS expression in the aortic tissue of hypertensive, but not of normal control rats. CONCLUSION: BCG vaccination could lower the blood pressure of hypertensive rats through activation of iNOS/NO pathway.

Objective To isolation and identify the pathogen of stem blight of Malvaceae.Methods The stems were collected from stem blight-diseased plants (J) and healthy ones (W) of Abelmoschus manihot (L.) Medic.in Yixing City of Jiangsu Province then cultured to isolate newborn mycelium.The pathogen isolated but unidentified were inoculated in stems of healthy plants of Abelmoschus manihot ( L.) Medic.and pathogenicity was verified.Finally, the pathogenic specie( s) was or were identified by morphological characteristic, rDNA-ITS analysis and polymerase chain reaction (PCR) method.Results The same fungus were excluded which were the same species in J and W, the three fungus of J2, J5 and J6 were acquired.J5 was preliminarily identified to have pathogenicity and it was Fusarium equiseti under the microscope.The genome DNA of J5 was amplified to a length of 524bp, and homology highly with Fusarium equiseti (100%).Conclusion The pathogen was identified as Fusarium equiseti.