Objective:The aim of this study was to examine whether PSA levels were affected by BMI and the influence of BMI on the clinical application of PSA.Methods:A total of 856 males who had routine health checkups between January 2006 and July 2007 in our hospital were recruited for this study.Those with a documented history of prostate carcinoma were excluded.Height,weight, and total PSA were measured.The BMl was calculated from measured height and weight [BMl=weight(kg)/height(m)2].Results:The serum PSA(ng/mL)levels were 2.97±0.15 in the normal weight group,2.53±0.12 in the overweight group,and 2.01±0.16 in the obese group(P＜0.05).A negative correlation was found between PSA and BMI(r=-0.130,P＜0.05)and weight(r=-0.142.P＜0.05).A positive correlation was found between PSA and age(r=0.306,P＜0.05).Con-clusion:BMl is associated with PSA.As BMI increases, serum PSA decreases.The current data suggest that such an influence should be considered in the clinical application of PSA.

Objective To establish an HPLC method for the determination of schisanhenol in plasma and to study the pharmacokinetics of schisanhenol in rats.Methods After sedimentation by methanol,plasma samples were then prepared based on a liquid-liquid extraction by ether.The extracted samples were analyzed by liquid chromatography.Schisanhenol was eluted on Eclipse XDB-C18 Agilent(250 mm?4.6 mm,5 ?m) column,using a mobile phase of acetonitrile-H2O(65∶35),and detected at 254 nm.The plasma concentration of schisanhenol in rats was determined after iv administration of 18 mg/kg,and the data were processed with the pharmacokinetic software 3P87.Results Calibration curves were linear over 0.1—2.5 ?g/mL (r2=0.999) and the LOD was 10 ng/mL.The recoveries of schisanhenol from plasma were between 88%—110%,and the RSD values of intra-day and interday assay were below 15%.After iv administration at 18 mg/kg,the schisanhenol concentration-time curve confirmed in a two-compartment model and the pharmacokinetic parameters of t1/2?,t1/2?,V,AUC,MRT were(0.22?0.11) h,(1.19?0.22) h,(12.81?2.91) L/kg,(1.32?0.19) ?g/mL/h,(1.51?0.24) h,respectively.Conclusion A reliable HPLC-DAD method is developed for the determination of schisanhenol in rat plasma and it is applicable to the in vivo analysis.

Objective: To observe effect of square dance exercises on symptom scores in menopausal women with coronary heart disease (CHD). Methods: A total of 39 menopausal CHD patients participated in square dance exercises, exercise intensity was 47.2%HRmax～58.1%HRmax（60~120 min/d）, 5~7 times/week, three months, and they were regard as dance group. Control group consisted of 42 CHD women of same age living in the same district, who didn’t take part in exercise. After enrollment, these two groups received assessments of Seattle angina questionnaire (SAQ) and frequent physical and mental symptoms of menopausal women self-rating scale (Kupperman self-rating scale) within 3d and after three months. Results: There were no significant difference in score of each item and total score of SAQ in first assessment between two groups (P>0.05 all). Compared with first assessment and control group after three months, there were significant increase in scores of most items and total score of SAQ [(66.40±15.16) scores vs. (67.18±13.93) scores vs. (85.15±16.30) scores] in dance exercises group, P<0.01~0.05. There were no significant difference in score of each item and total score of Kupperman scale in first assessment between two groups (P>0.05 all). Compared with first assessment and control group after three months, there were significant decrease in scores of most items and total score of Kupperman scale [(29.40±6.51) scores vs. (29.73±6.26) scores vs. (23.65±6.29) scores] in dance exercises group, P<0.01~0.05. Conclusion: Square dance exercises may significantly decrease angina pectoris symptom and menopausal physical and mental symptoms in menopausal women with coronary heart disease.

Objective To investigate the effect of ulinastatin (UTI) on hepatic ischemia-reperfusion injury in rats. Methods Totally 24 adult Sprague-Dawley rats were randomly divided into 3 groups: sham-operated control group (SO group), ischemia-reperfusion group (I/R group) and ulinastatin group (UTI group). Liver in I/R group underwent 1 h of reperfasion after 30 min of ischemia. In UTI group, UTI (2×104 U/kg) was administered to rats 30 min before modeling. The levels of alanine aminotransferase, aspartate aminotransferase and tumor necrosis factor-alpha (TNF-α) in serum were measured and the levels of nitric oxide and malondialdehyde in liver were determined. The histological changes of liver were observed. Results The levels of alanine aminotransferase, aspartate aminotransferase and TNF-α in serum were significantly increased in I/R group compared with those in UTI group (P<0.05). The levels of nitric oxide and malondialdehyde in liver were significantly higher in I/R group than in UTI group (P<0.05).Histological examination of liver indicated that the damages were more severe in I/R group than in UTI group.Conclusion UTI has the ability to inhibit the production of TNF-α and oxyradical, and ameliorate microcirculatory dysfunction in rats with hepatic ischemia-reperfasion injury.

Objective To study the preparation of volatile oils obtained from asarum and lily magnolia complexed with ?-cyclodextrin and hydroxypropyl-?-cyclodextrin in Xuetie Dingchuan plaster,and to study its impacts of transdermal absorption.Methods The volatile oil was complexed with ?-cyclodextrin and hydroxypropyl-?-cyclodextrin.GC method was used in the determination of volatile oils complexed with ?-cyclodextrin and hydroxypropyl-?-cyclodextrin to investigate the impacts of transdermal absorption.Results The accumulation transmission of the volatile oils complexed with ?-cyclodextrin was superior to that with hydroxypropyl-?-cyclodextrin.Conclusion The inclusion of volatile oils with ?-cyclodextrin can enhance drug penetration via skin.

Objective To investigate the relationship between the cerebral blood flow filling condition and prognosis of patients with acute severe cerebral infarction.Methods The clinical data and MR features of 10 cases of acute severe cerebral infarction were retrospectively analyzed.Results The regional cerebral blood flow(rCBF) and regional cerebral blood volume(rCBV) of 9 cases(90%) in the infarcted side decreased significantly.The reduced percents of the infarcted side reached 64.1% and 49.7% respectively.There were significant differences between two opposite sides(P=0.001,P=0.01).The MRA results showed great vessels(arteria carotis interna or arteria cerebri media) in the infarcted side were all obliterated in the all patients,and simultaneous great vessels in opposite side were narrow or occlusion in 7 cases(70%).2 cases died;and 8 cases were serious disability.Conclusions The cerebral blood flow filling of the acute severe cerebral infarction patients decrease significantly.The prognosis of the patients is quite bad.

Objective Mapping the responsible gene for congenital nuclear cataract in a family for five generations in Yantai City,Shandong Province,China.Methods Family history and clinical data were recorded.9 unaffected members and 13 affected members in this family were involved in the study.The genes of all the involved members were amplified by polymerase chain reaction(PCR).28 microsatellite polymorphism in the 15 reported disease loci were used as genetic markers.The PCR products from each DNA sample were separated on a 6% polyacrylamide gel and analyzed.Allele-sharing analysis was carried out for exclusion,and linkage analysis was calculated with the LINKAGE(Version 5.1)package.Direct sequencing was used for GJA3 gene.Results The clinical phenotype in this family was isolated congenital nuclear cataract,the pathogenic nutation of the phenotype of which has not been reported yet.For all the 28 markers around the 15 candidate loci,there was no allele-sharing between the affected family members.At the 0.00 recombination frequency,the LOD score was-∝ in 27 of the 28 microsatellite markers with exception of D11S898.No GJA3 gene mutation was found.It indicated that there was no linkage between these markers and the pathogenic gene in this family.Conclusion The responsible gene for the congenital nuclear cataract in this family is not located on the 15 reported loci,which further indicates the clinically and genetically heterogeneity of inherited cataract,and an important clue is provided for finding more cataract responsible genes.The pathogenic gene in this family should be identified through extensive scanning of genes.

[Objective]To investigate the effect of high energy shock waves on the expression of BMP-2 in adult rabbits with necrotic femoral head,and to explore the mechanism of avascular femoral head necrosis treated with high energy shock waves.[Methods]Thirty eight male adult New Zealand white rabbits,weighting 3.0 to 4.0 kg(average weight 3.3 kg) were used to establish animal model of avascular femoral head necrosis.Bilateral femoral head necrosis was induced by methylprednisolone and lipopolysaccharide.The left hind limbs(treatment side) of all rabbits received shock wave treatment of the femoral heads and the right limbs(control side) of all rabbits received no shock wave treatment.The animals were killed at 24 hours and 1(n=6),2(n=6),4(n=6),8(n=6),and 12(n=6) weeks after shock wave therapy.The femoral heads were harvested and freed of soft tissue and cartilage.They were assigned to immunohistochemistry and quantitative real-time reverse transcriptase PCR.[Results]Compared with the contralateral control without shock wave treatment,the BMP-2 mRNA expression levels increased to a peak at 1 week after the shock wave treatment and remained high at 24 hours and 1、 2、 4、 8 weeks after the shock wave treatment.At 4,8 and 12 weeks after shock wave treatment,the average percentage of staining area of the femoral heads in the treatment side were significantly higher than those in the control side(P

By using thrombolysis therapy,34 patients suffering from acute myocardial infarction were treated.And by comparing iron.zinc,copper,manganese and selenium contents in serum of 34 patients suffering from acute myocardial infarction with that of control group and the contents between before-treating and after-treating,following results are revealed:before treating,iron,zinc,copper contents in serum of the patients increased while manganese and selenium contents decreased,there is great difference between the contents of the patients and control group(P

Medical rescue is the most important key point for deciding disaster prognosis. But there are not systemic and special mode of disaster medical education. This article expounds the concept and features of disaster medicine, and puts forward the idea that the training routes of talents in disaster medicine should be included in undergraduates, vocational and continuing education.