Objective To study the relationship between hyperhomocysteic acid(HHcy) and artherosclerosis.Methods 20 male rabbits were randomly divided into control and experiment groups(n=10).HHcy models were made by hypodermical injection of DL-methionine for 7 weeks.After 7 weeks homocysteic acid(Hcy),IL-1?,IL-6,and IL-8 in serum of rabbits in two groups were measured by enzyme linked immunosorbent assay(ELISA).And the pathomorphological changes of aorta were observed with HE staining.The number of NF-?B positive cells in the aorta were counted by immunohistochemical method.Results The levels of Hcy,IL-1?,IL-6 and IL-8 in experiment group were higher than those in control group(P

0.05). There were 7 cased in the polypropylene mesh group had abdominal discomfort due to stiffness, which higher than that of the expanded polytetrafluroethylene patch and control groups (P 0.05). CONCLUSION: Both prosthetic patches are safe and effective when used in the repair of inguinal hernia in aged patients with low recurrence rate, especially, the expanded polytetrafluroethylene patch, which is more comfortable than polypropylene mesh.

Objective To describe the disease's spectrum of fever patients,especially to analyze the epidemic characterization of infectious diseases,to offer the proof for fever department in screening of infectious diseases.Methods A total of 15081 patients were analyed for patients presenting to the Infectious Diseases Department,People's Hospital,Peking University,between June 2005 to May 2006.A retrospective study was used to analyze the disease's spectrum and epidemic characterization.Results Among the disease's spectrum of fever patients,the first leading cause was respiratory diseases(78.22%),followed by infection with the special patients(10.50%)、acute gastroenteritis(3.57%)、the infection at other site with etiological factor determined(2.82%)、infectious diseases(2.30%)、urinary system infection(2.06%) and fever of unknown origin(0.53%),respectively;Among the spectrum of infectious diseases,the first leading cause was infectious diarrheal disease(29.97%),followed by measles(24.78%)、lung tuberculosis(11.24%).Conclusion The spectrum of fever is complex.To obtain the epidemic characterization of infectious diseases,it's helpful to the fever department to screen and prevent the infectious diseases.

By using thrombolysis therapy,34 patients suffering from acute myocardial infarction were treated.And by comparing iron.zinc,copper,manganese and selenium contents in serum of 34 patients suffering from acute myocardial infarction with that of control group and the contents between before-treating and after-treating,following results are revealed:before treating,iron,zinc,copper contents in serum of the patients increased while manganese and selenium contents decreased,there is great difference between the contents of the patients and control group(P

Objective To investigate the effect of donors' activating killer cell immunoglobulin-like receptor (aKIR) on recipients prognosis post haemopoietic stem cell transplantation (HSCT).Methods Fifty-nine cases of related HLA full matched HSCT were studied. Sequence specific primer polymerase chain reaction (SSP-PCR) was used to type donors' KIR. Virus,bacterium,and fungi infection as well as bleeding,relapse,survival were observed in the recipients post transplantation. Relationship between aKIR and clinical indexes mentioned above were analyzed.Results Donors' aKIR showed no beneficial effects on recipients' virus and bacterium infection. Furthermore,the incidence of fungi infection was increased in HSCT if donors expressed KIR3DS1 (?2= 4.804 ,P= 0.028 ). No significant difference was found in survival curve between donor aKIR positive HSCT or negative one (KIR2DS1+/-: P= 0.651 ; KIR2DS2+/-: P= 0.847 ; KIR3DS1+/-: P= 0.341 ). No effect of donor's aKIR on relapse was observed,too.Conclusion In Guangdong Han,so far as related HLA full matched HSCT is concerned,no improvement in recipient prognosis due to donor's aKIR is verified.

To evaluate the influences of sclerotherapy and band ligation on esophageal motility, EVS and EVL were measured before and after treatment respectively. After sclerotherapy, lower esophageal sphincter pressure (LESP)and residual pressure were decreased while relaxa tion was increased , relaxation duration became shorter; band ligation only influenced LESP.After EVS, the amplitude of motor waves in the sites of EVS and lower esophagus body was reduced ,and peristaltic wave duration was prolonged ,velocity of propagating contractions became faster , band ligation made the peristaltic wave duration in the sites of EVS and lower esophagus body become shorter, abnormal contractions increased. After EVS, antiacid medicine should be ased to reduced pH of gastric and raised LESP, and drugs to increase esophageal motility should be used after band ligation.

Objective To investigate the effects of magnetic stimulation (MS) on the proliferation and differentiation of endogenous neural stem cells (NSCs)/progenitor cells after spinal cord injury (SCI) in rats. Methods Forty-six Wistar rats were used, of which 40 were used to make an animal model of spinal cord injury (SCI) by administering a 10 g x 12.5 cm impact at the T8 level. The other 6 served as the normal controls. The SCI model rats were evenly divided into a magnetic stimulation (MS) group ( n = 20) and a control group ( n = 20). The rats in the MS group received 0.5 Hz and 1.44 T magnetic stimulation 24 h post injury, then 30 pulses per day for 7 days. The rats in the other groups were not exposed to MS. The scale of Basso, Beatti and Bresnahan (BBB) was used to assess hindlimb neurological function. Rats were sacrificed at the 24th hour, and at the 1st, 4th and 8th weeks after SCI. The ratio of nestin to microtubule associated protein 2 (MAP2)/nestin in the cells of the spinal cord was determined by immunofluorescence. Results The BBB scores in the MS group were signifi-cantly higher than those of the control group at 1, 4 and 8 weeks post SCI. Nestin and the MAP2/nestin ratios were mild in the normal spinal cords, but increased after SCI. They were higher in the MS group than that in the control groups at all time points. Conclusions MS can promote nestin expression in the spinal cord after SCI and facili-tate neural differentiation.

Objective To compare the functional profiles of organ transplant patients using the International Classification of Functioning,Disability,and Health (ICF) core set.Methods The patients were enrolled 5 to 10 days after discharge following an organ transplant.The Functional Independence Measure (FIM),Barthel Index (BI) and the ICF core set were used to assess them.Analysis was conducted by grouping the kidney transplant patients (group A) separately from the heart,lung and liver transplant patients (group B).The prevalence of sevcre impairment in each group was calculated and compared.Results Average FIM and BI secores were both significantly higher in group A than in group B.No severe or total impairment was observed in group A,but in group B,the prevalence of 5 categories (ie.b455,b730,s430,d415 and d450) was significantly greater than in group A.The most prevalent were poor exercise tolerance (b455,56.8％),low muscle power (b730,54.5％),difficulty in maintaining a body position (d415,54.5％),and impaired walking (d450,45％) With regard to the environment factors,the prevalence of e110 (products or substances for personal consumption) and e120 (products and technology for personal use in daily living) were both significantly different in the two groups.Conclusion Heart,lung and liver transplant patients transplant demonstrated more impairments than the kidney transplant patients.All the organ transplant patients deserve early evaluation for detecting any possible impairment.

Objective:To discuss the clinical significance of overlapping infection of HGV in blood donorsand viral hepatitis. Methods :HGV-RNA was detected by reverse transcription-polymerase chain reaction.Results :The infectious rate of HGV in blood donors was 4% ,that in the patients with HBV and HCV was13. 9% and 15.8% respectively. Conclusion:Our results indicate that the HGV infection was widespread.Further study of immune response and status of viral replication in the liver tissue in overlapping infectionwith HBV and HCV,was needed.

Gene deletion vector pLJ04(pKC1139∷bkdF+bkdH)was used to disrupt bkdFGH in Streptomyces avermitilis 76-02-e,an industrial producer of anthelmintic avermectin.The disruptants were confirmed by PCR.Shake flask experiment and HPLC analysis showed that the mutant lost the ability to produce avermectins.As it is expected,the mutant,named S.avermitilis bkd76-3,could restore the ability of producing avermectins when the feeding of methylbutyric acid and isobutyric acid to its fermentations was carried out.The addition of cyclohexanecarboxylic acid(CHC)into fermentations of the S.avermitilis bkd76-3 allowed for production of four components,two of which was confirmed as CHC-B1 and CHC-A2 by LC/MS analysis,respectively.