Objective:To establish the specific chromatogram of Fufang Shexiang injection and simultaneously determine five com-ponents( menthol,borneol,patchouli alcohol,muscone,α-asarone) in the injection by GC. Methods:The sample solution was extracted by a solid-phase extraction method. The separation was performed on a DB-WAX GC capillary column with an FID detection. Results:The specific chromatogram had six common peaks, and the similarity was above 0. 91. The five chemical components(menthol,borne-ol,patchouli alcohol,muscone,α-asarone) were well separated and with good linear relationship, the recoveries were between 95. 1 and 102. 6%, and RSD was less than 3. 0%. Conclusion:The method is simple, accurate and reproducible. The specific chromatogram combined with multi-component determination can reflect the overall quality of Fufang Shexiang injection, which can be used for the quality control of the preparation.

Objective To perform laboratory detection and molecular traceability analysis on a case of imported kala-azar in Shenzhen to determine the infection strain. Methods Bone marrow puncture fluid and blood samples from a case of kala-azar in Shenzhen were collected for laboratory tests. The patient's bone marrow puncture fluid smears were stained with Giemsa and examined under a microscope. Blood samples were examined for antibodies using the rk39 visceral leishmania rapid diagnostic reagent. Whole blood DNA was extracted, and the ITS-1 sequence was amplified by PCR, sequenced and aligned, and a phylogenetic tree was constructed based on the ITS-1 sequence. Results Microscopic examination of the patient's bone marrow smears revealed a large number of Leishmania amastigotes without flagella, confirming the diagnosis of kala-azar. The patient's blood was tested positive with the rk39 rapid diagnostic reagent, and PCR amplification yielded an ITS-1 gene product sequence that matched the expected size. Sequence alignment with the NCBI database showed 100% sequence similarity with the ITS-1 gene sequence of Leishmania infantum, confirming the infecting strain as Leishmania infantum. Phylogenetic tree construction of the amplified ITS-1 sequence revealed clustering into a clade with Leishmania infantum , and close to KC347299, one of the reference sequence selected. Conclusions The case of kala-azar in Shenzhen was caused by Leishmania infantum. Kala-azar still occurs in China, so the diagnostic technology of medical personnel in non-epidemic areas should be strengthened so that they can actively use new diagnostic technologies to assist in diagnosis, thus improving their prevention and control ability of Leishmania parasites.

Objective: To understand secular trend of blood pressure among Han ethnic students aged 7-18 years in Ningxia from 2000 to 2014，and to provide preliminary evidence for hypertension prevention and control. Methods: Data of blood pressure, height, weight of Han students aged 7-18 years in Ningxia were collected in the National Students Physical Fitness and Health Survey during 2000-2014 years were analyzed by using SPSS 21.0. Results: From 2000 to 2014, the systolic pressure of the Han students in Ningxia showed a decreasing-increasing trend(F=357.44, P<0.05), with an average decrease of 3.37 mm Hg; diastolic pressure showed a decreasing-increasing trend(F=172.95, P<0.05), with an average decrease of 4.18 mm Hg; pulse pressure showed a decreasing-increasing trend(F=311.86, P<0.05), with an average decrease of 1.98 mm Hg. The body mass index of the Han nationality students in Ningxia was on the rise(F=128.15, P<0.05). The detection rates of high blood pressure, high systolic blood pressure and high diastolic blood pressure increased by 1.0, 0.4 and 0.9 percentage. Conclusion Blood pressure in Ningxia Han students aged 7-18 years increases gradually in recent years, which warrants further attention. Health education and health promotion is needed to prevent the occurrence of hypertension.