Objective: To explore the inhibitory effect of pharmacological ligand to PPAR?-rosiglitazone on smooth muscle cell proliferation of rat aorta.Methods: smooth muscle cells were prepared from rat aortas using explant technique and identified by their specific growth characteristic and immunocytochemistry.The fifth passage cells were made quiescent by serum starvation for 24 hours,and then cells were stimulated with PDGF-BB or bFGF(20ng/ml) for 24 hours in the presence or absence of rosiglitazone(1~10umol/L),pretreated for 30 minutes.MTT assay was applied to assess cellular viability.Flow cytometry was used for cell cycle analysis.Results: Under microscope,the cultured cells possessed "peak and valley" characteristics for smooth muscle cell.Immunocytochemical staining with specific mAb against ?-actin demonstrated these cells were positive.Smooth muscle cell proliferation was induced by PDGF-BB or bFGF with an increase A490 in MTT.The cell number was reduced in the S phase and increased in the G0/G1 phase.Cell proliferation and transition from G0/G1 to S were inhibited using pretreatment of rosiglitazone in a dose-dependent manner.Conclusion: Rosiglitazone has inhibitory influence on vascular smooth muscle cell proliferation induced by growth factors.

Objective To investigate the safety of novopen needle after repeated uses in order to save the expenses of patients. Methods 60 diabetic patients undergoing insulin therapy were random-ized into the common temperature group and the refrigerated storage group. The novopens were stored at common temperature in a clean and dry place, while the refrigerated storage group was stored in cold clos-et of a refrigerator. Oropharyngeal swab bacterial culture was used to compare the incidence of infection in two groups on the first, third, fifth, and seventh days. Results There was no bacterial growth in either storage condition. Conclusions The novopen needle can be repeatedly used in clinic work.But they must be used individually. Storage at common temperature or in a refrigerater can reach the same effect.

Based on the questionnaires completed by 985 medical students,this article finds out that female students value more on the factors of health care and development when choosing a job,while male students are more confident in selecting goals and making career plans.Students from different family backgrounds have great difference when facing the elements such as reputation,social status and selfevaluation.Students from different majors take quite different opinions to health care,self evaluation,career information and career planning.There is little difference among different grades of medical students.Vocational values correlate positively with career decision-making self-efficacy.

ObjectiveTo investigate the influencing factors for persevering in pelvic floor muscle exercise in puerperal women and nursing countenneasures.MethodsThe influencing factors for persevering in pelvic floor muscle exercise in 120 puerperal women before June 2010 were analyzed,and they were set as the control group.According to the analysis results,targeted nursing countermeasures were developed,comprehensive care approach that combined nursing intervention with health education were applied in 116 cases of puerperal women from June 2010 to June 2011,who were set as the observation group.The situation of recovery for maternal pelvic floor muscle tension was observed.ResultsThere were four main factors that affected maternal pelvic floor muscle exercise:pelvic floor muscle exercise is not inspected as post-natal visits items,obstetric nurses did not carry out necessary propaganda,did not understand the importance of pelvic floor muscle exercise and lack of knowledge among the puerperal women.The test results for pelvic floor muscle tension were as followed:in the observation group Ⅰ degree was in 9 cases,accounting for 7.76％; Ⅱ degree was in 31 cases,accounting for 26.72％; Ⅲ degree was in 76 cases,accounting for 65.52％.In the control group,I degree was in 35 cases,accounting for 29.17 ％; Ⅱ degree was in 44 cases,accounting for 36.67％; Ⅲ degree was in 41 cases,accounting for 34.17％.The recovery effect of pelvic floor muscle tension in the observation group was obviously better than the control group,the difference was significant.ConclusionsFactors influencing the puerperal women in persevering in pelvic floor muscle exercise was various,personalized care and intervention according to the influencing factors can improve compliance of pelvic floor muscle exercise for puerperal women,contribute to the recovery of pelvic floor muscle tension.

Gentisate (2,5 dihydroxybenzoate) pathway is one of three typical routes for bacterial aromatic catabolism Although it is less common than either of the extensively studied pathways through catechols, the gentisate pathway also plays an important role in bacterial aromatic catabolism This article reviews recent developments of this pathway, both in biochemistry and molecular biology

The constitutional medical thoughts in Inner Canon of Huangdi is the foundation for TCM constitution theory, it discusses the influence of constitution on disease in detail. Constitution factors determine the disease onset or not; Constitution factors determine the tendency and character of disease. Constitution factors effect the changes of pathogenesis, the character of syndrome, the therapeutic principle and methods; Constitution factors effect the turnover and prognosis. Constitution therapies are profit to correct the partial body, preventing the development of disease and heal the disease.

Lung cancer is one of the most common malignant tumors in the world .However,its pathogen-esis is still unclear .With the deep research of related signal pathways underlying the development ,progression and prognosis of lung cancer , we find that the mTOR signaling pathway , JAK-STAT pathway , Notch signaling and IGF have been identified to play a major role in the growth ,proliferation,differentiation,apoptosis and invasion of tumor cells.Therefore this paper is to review the effects of the above several pathways ,which may prove a new ba-sis for the pathogenesis and therapy of lung cancer .

With the development of oral science and technology,combined surgical and orthodontic treatment becomes a new developing subject.This article clarifies the most important facets of the ethical problems for the successful combined surgical and orthodontic treatment;the author thinks noble medical morals, excellent medical technology,scrupulous science style and responsible attitude as the important subjective factors for this successful treatment.

OBJECTIVETo investigate the changes in the craniofacial morphology ofdeepbite from childhood to adulthood using cross-sectional methods. To analyze the maxillofacial characteristics of adult deepbite.

METHODSThe sample included 159 children (with average age of 12.47 years old) and 81 adults (with average age of21.76 years old) with class III deepbite. The control group consisted of51 normal individuals (with average age of 18.41 years old). Lateral cephalometric radiographs were taken and recorded in a computer through a scanner. Cephalometric measurements were conducted by using Winceph 7.0 software, and results were analyzed with SPSS 12.0 software.

RESULTSSignificant differences between child and adult deepbite were observed in the following: N-ANS, ANS-Me, A-Ms, A-Ptm, Wits, Mo-Ms, Ii-Ii, A-B plane angle to the mandibular plane angle, Gonial angle, L1 to NB length, overjet, mandibular body to anterior cranial base, Mo-Mi, posterior facial height, U1 to NA length, Pog-Go, Cd-Go, occlusion plane angle to SN, and U1 to SN. In addition, significant differences between adult deepbite and normal occlusion were observed in SNB, ANB, convexity, APDI, ODI, Wits, A-B plane to mandibular plane, Gonial angle, overjet, mandibular body to anterior cranial base, S-Ptm, Mo-Mi U1 to SN; Pog-Go, Cd-Go, posterior cranial base, and occlusion plane to SN and posterior facial height.

CONCLUSIONDeepbite patients have certain growth potential after puberty, but the sagittal relationship of their jaws exhibits no improvement. Adult deepbite patients exhibit significant problems in the vertical and sagittal jaw positions.

Adult ; Bone and Bones ; Cephalometry ; Child ; Cross-Sectional Studies ; Dental Occlusion ; Female ; Humans ; Male ; Mandible ; Sexual Maturation

Mastoparan(MAS) and α-latrotoxin(α-LTX) are two kinds of insulinotropic peptides obtained from insect toxins which can interact with islet β-cells and induce insulin secretion. The signal mechanism of these insulinotropic peptides regulating insulin-releasing attracts notable attention and has been elucidated more and more details. MAS mainly acts on the molecular components of exocytosis at a late stage. Insulin secretion induced by MAS is obviously dependent on GTP, which subsequently activates G-protein located on insulin secretion granules(ISG), or activates the Rho subfamily of small G proteins to evoke exocytosis and sensitize fusion machinery. The MAS stimulated insulin-releasing activity can be augmented by nutrients. However, its effect is not Ca~(2+) dependent. There are two regulatory pathway triggered by α-LTX: one way is pore formation caused through plasma membrane, another way is the transmembrane signal transduction evoked by cytosolic second messengers. Tetrameri complexs assembled at high concentration of α-LTX toxin or in the presence of extracellular Ca~(2+), can insert α-LTX into plasma membrane to form Ca~(2+) permeable channels and trigger Ca~(2+)-dependent secretion. By binding to transmembrane receptors and activating phospholipase C, α-LTX induces the generation of second messenger DAG and IP3. IP3 triggers Ca~(2+) influx and subsequently activates CaMK pathway, however, DAG also activates PKC pathway to increase insulin release.