This paper reports one case of Gongylonema pulchrum infection in the esophagus in human.

Objective To study the association of SNP of the AdipoR1 gene with T2DM in Xi’an population. Methods The amplification refractory mutation system(ARMS) analysis and gene sequencing were used to investigate the AdipoR1 gene polymorphism in 100 type 2 diabetics and 84 normal control subjects. Results (1) The genotype and allele frequencies of -106A/G, 5843A/G were not significantly different between type 2 diabetics and normal control subjects. (2) The diagnosis age of diabetics was significantly younger in AdipoR1 5843GG genotype group than in other genotype groups. Conclusions The data implicate that the AdipoR1 gene -106A/G and 5843A/G polymorphism may be not associated with pathogenesis of T2DM. AdipoR1 5843 GG genotype may be associated with the earlier diagnosis of T2DM

Objective To observe the therapeutic effects of dietary,exercise intervention combined with Rou-ganjiangzhi capsule on non -alcoholic fatty liver disease.Methods A randomized,double -blinded and placebo drug parallel controlled method was used in this study.Sixty patients were enrolled into the study and randomly assigned into treatment group and control group.The patients of treatment group were taken with Rouganjiangzhi capsule combined behavior intervention.The patients of control group were taken with placebo drug combined behavior inter-vention.Each patient were accepted 12 weeks of the therapy.After treatment,the clinical symptoms,liver function, blood lipids,indicators of changes in imaging were detected,and the results of each group statistically were analyzed. Results After 12 weeks treatment,five patients were rejected,and the clinical symptoms,body mass index (BMI), waist -hip ratio were markedly improved in each group:[(1.02 ±1.08)points,(26.29 ±2.54),(0.92 ±0.04), (0.81 ±0.95),(26.58 ±3.11)and (0.91 ±0.04)]compared with[(3.62 ±0.96)points,(27.63 ±2.50),(0.94 ± 0.03),(3.60 ±0.95),(28.22 ±3.14),(0.95 ±0.03)(t =4.74,2.64,0.82,4.62,3.61,0.84,all P <0.05)]. After the treatment,the liver function (ALT,GGT)and cholesterol (TC)of treatment group decreased significantly than that before treatment:[(75.28 ±25.19)IU /L,(48.41 ±38.42)IU /L,(3.02 ±0.85)IU /L],compared with [(127.46 ±52.42)IU /L,(48.41 ±38.42)IU /L,(4.63 ±0.69)IU /L (t =2.54,2.18,2.42,all P <0.05)],while the liver function (ALT,AST,GGT)and blood lipids(TC,TG)of control group had no significantly decreased.The liver and spleen CT ratio raised apparently in treatment group than control group (0.86 ±0.24),(0.71 ±0.20)(t =0.83, P <0.05).Conclusion Rouganjiangzhi capsule achieve the good therapeutic effect on non -alcoholic fatty liver.

Objective To select the optimum ultrasonic extracting method of polysaccharides from Rosa Laevigatae Michx in Guizhou. Methods Independent variables were solid-liquid ratio, extraction time and extraction temperature, dependent variable was extraction rate ratio of polysaccharides from Rosa Laevigatae Michx.Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables. Response surface methodology was used to optimize the process of extraction.Prediction was carried out through comparing the observed and predicted value. Results The optimum extraction condition for the polysaccharides was as follows: extracting at 75 ℃ for 60 min and ratio of liquid to solid 30??1 ( mL??g ) . The average extraction yield of polysaccharides was 37. 54% under the optimum extraction condition. Conclusion This optimized extraction process is simple and reliable for the extraction of polysaccharides from Rosa Laevigatae Michx in Guizhou.

Objective The aim of this study is to investigate the effects of the initial amount of total RNA input,adapter dilution and PCR amplification cycles on the results of small RNA library preparation and high-throughput sequencing.Methods Based on the sequencing reads number,the number of detected miRNAs and the accuracy of quantitative detection,we explored the effects of initial Total RNA,dilution ratio and PCR cycles on the quality of microRNA library preparation and high-throughput sequencing,respectively.Results For libraries preparation of the normal RNA input(1 μg),the adapter dilution combined with 22 PCR cycles could gain the best quality of sequencing.In the low-input libraries(10 ng),adapter dilution and increased PCR cycles would also improve the quality of sequencing,but as compared with the 1 μg library,there was lower correlation with microarray quantitative results in general.Conclusions The initial amount of RNAs input has the biggest effects on the quality of sequencing,and the quantity accuracy rate of low-input libraries is lower than the normal libraries generally.Increasing the PCR amplification cycles properly is indispensable to low-input libraries.

Embryonal tumors of central nervous system (CNS) are highly invasive malignant tumors that mainly affect infants.Even if the current active treatment strategies are given, the prognosis is still very poor.The 2016 World Health Organization(WHO) classification integrates the genetic information of diseases with histopathology, so as to make the classification and diagnosis of these tumors more accurate.In the new classification, embryonal tumors of CNS include the most common medulloblastoma, and the rare embryonal tumor with multilayered rosettes, C19MC-altered, embryonal tumor with multilayered rosettes, not otherwise specified (NOS), medulloepithelioma, CNS neuroblastoma, CNS ganglioneuroblastoma, CNS embryonal tumor, NOS, atypical teratoid rhabdoid tumor, and CNS embryonal tumor with rhabdoid features.Further study and exploration on the biological nature of these rare brain tumors will provide scientific basis for new treatment methods.

The integrated course centered on organ system has been implemented for several years in Huazhong university of science and technology.Basic medicine courses were divided into three modules.Medical introduction Ⅰ was the first one which integrated nine basic medicine courses.Reform on teaching methods,teaching content,teacher training and experiment teaching were conducted and initial results were achieved.

Objective:To select items to compose the final scale from 101-item pool for assessment of postnatal quality of life which was developed by the authors before. Methods: Ten experts in maternal and child health scored the importance of every item. A total of 200 women at 0-12 months after childbirth who took their babies for vaccination or clinic services were investigated by the item pool, and 10% of them were retested in one month. Based on these data, items in the item pool were screened by following principles respectively: score of item importance, factor loading, variation coefficient, Cronbach Alpha, correlation coefficient of item with domains (2 types) and test-retest correlation coefficient. Then we summarized the screening results of these methods, and retained the items which could be selected in at least five methods. Results: The numbers of items deleted in these seven screening methods respectively were: 39 items in experts scoring method; 43 items in factor analysis method; 49 items in variation analysis method; 50 items in Cronbach Alpha method; 26 items in correlation coefficient method 1; 9 items in correlation coefficient method 2; and 11 items in test-retest method. After summarizing these screening results, 60 items were selected. In order to simplify the scale,only kept 2 items in each aspect which had more frequency retained. Finally, 40 items remained in the postnatal quality of life scale. Conclusion: This is the first Chinese scale for evaluating postnatal quality of life of women. It needs to establish the validity and reliability in the future.

Humans ; Mycoplasma pneumoniae ; Pneumonia, Mycoplasma ; epidemiology

Objective To analyze and discuss the value and significance of coronary artery bypass grafts in the same period of heart valve replacement in the process of the coronary heart disease with heart valve disease treatment, in order to improve the effect of treatment and reduce the incidence of adverse events. Methods 60 cases with coronary heart disease with heart valve disease , which admitted in our hospital during February 2013-February 2015, were selected as the research objectaccording to random number table method and divided into the-experimental group and the control group , each group of 30 cases according to the principle of double blind control. The experimental group was given the coronary artery bypass grafts in the same period of heart valve replacement and the control group was given the coronary artery bypass grafts without heart valve replacement. The curative ef-fects of different schemes and effects on cardiac function after surgery of the two groups were compared. Results After statistical analysis found thatthe observation indexes of the control group was significantly increased compared with the experimental group, the difference was significant statistically significant (P<0.05). The postoperativecar-diac function indexes of the experimental group was significantly improved then control group , the difference was significant statistically significant (P<0.05). Conclusion Coronary heart disease with heart valve disease in the process of treatment can also take heart valve surgery combined coronary artery bypass surgery. By preoperative and postoperative symptomatic treatment, intraoperative protect myocardium, can effectively prevent and reduce the op-eration of various complications, which is worthy of clinical popularization and application.