This paper reports one case of Gongylonema pulchrum infection in the esophagus in human.

Objective The aim of this study is to investigate the effects of the initial amount of total RNA input,adapter dilution and PCR amplification cycles on the results of small RNA library preparation and high-throughput sequencing.Methods Based on the sequencing reads number,the number of detected miRNAs and the accuracy of quantitative detection,we explored the effects of initial Total RNA,dilution ratio and PCR cycles on the quality of microRNA library preparation and high-throughput sequencing,respectively.Results For libraries preparation of the normal RNA input(1 μg),the adapter dilution combined with 22 PCR cycles could gain the best quality of sequencing.In the low-input libraries(10 ng),adapter dilution and increased PCR cycles would also improve the quality of sequencing,but as compared with the 1 μg library,there was lower correlation with microarray quantitative results in general.Conclusions The initial amount of RNAs input has the biggest effects on the quality of sequencing,and the quantity accuracy rate of low-input libraries is lower than the normal libraries generally.Increasing the PCR amplification cycles properly is indispensable to low-input libraries.

Objective To select the optimum ultrasonic extracting method of polysaccharides from Rosa Laevigatae Michx in Guizhou. Methods Independent variables were solid-liquid ratio, extraction time and extraction temperature, dependent variable was extraction rate ratio of polysaccharides from Rosa Laevigatae Michx.Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables. Response surface methodology was used to optimize the process of extraction.Prediction was carried out through comparing the observed and predicted value. Results The optimum extraction condition for the polysaccharides was as follows: extracting at 75 ℃ for 60 min and ratio of liquid to solid 30??1 ( mL??g ) . The average extraction yield of polysaccharides was 37. 54% under the optimum extraction condition. Conclusion This optimized extraction process is simple and reliable for the extraction of polysaccharides from Rosa Laevigatae Michx in Guizhou.

Objective To observe the therapeutic effects of dietary,exercise intervention combined with Rou-ganjiangzhi capsule on non -alcoholic fatty liver disease.Methods A randomized,double -blinded and placebo drug parallel controlled method was used in this study.Sixty patients were enrolled into the study and randomly assigned into treatment group and control group.The patients of treatment group were taken with Rouganjiangzhi capsule combined behavior intervention.The patients of control group were taken with placebo drug combined behavior inter-vention.Each patient were accepted 12 weeks of the therapy.After treatment,the clinical symptoms,liver function, blood lipids,indicators of changes in imaging were detected,and the results of each group statistically were analyzed. Results After 12 weeks treatment,five patients were rejected,and the clinical symptoms,body mass index (BMI), waist -hip ratio were markedly improved in each group:[(1.02 ±1.08)points,(26.29 ±2.54),(0.92 ±0.04), (0.81 ±0.95),(26.58 ±3.11)and (0.91 ±0.04)]compared with[(3.62 ±0.96)points,(27.63 ±2.50),(0.94 ± 0.03),(3.60 ±0.95),(28.22 ±3.14),(0.95 ±0.03)(t =4.74,2.64,0.82,4.62,3.61,0.84,all P <0.05)]. After the treatment,the liver function (ALT,GGT)and cholesterol (TC)of treatment group decreased significantly than that before treatment:[(75.28 ±25.19)IU /L,(48.41 ±38.42)IU /L,(3.02 ±0.85)IU /L],compared with [(127.46 ±52.42)IU /L,(48.41 ±38.42)IU /L,(4.63 ±0.69)IU /L (t =2.54,2.18,2.42,all P <0.05)],while the liver function (ALT,AST,GGT)and blood lipids(TC,TG)of control group had no significantly decreased.The liver and spleen CT ratio raised apparently in treatment group than control group (0.86 ±0.24),(0.71 ±0.20)(t =0.83, P <0.05).Conclusion Rouganjiangzhi capsule achieve the good therapeutic effect on non -alcoholic fatty liver.

Objective To study the association of SNP of the AdipoR1 gene with T2DM in Xi’an population. Methods The amplification refractory mutation system(ARMS) analysis and gene sequencing were used to investigate the AdipoR1 gene polymorphism in 100 type 2 diabetics and 84 normal control subjects. Results (1) The genotype and allele frequencies of -106A/G, 5843A/G were not significantly different between type 2 diabetics and normal control subjects. (2) The diagnosis age of diabetics was significantly younger in AdipoR1 5843GG genotype group than in other genotype groups. Conclusions The data implicate that the AdipoR1 gene -106A/G and 5843A/G polymorphism may be not associated with pathogenesis of T2DM. AdipoR1 5843 GG genotype may be associated with the earlier diagnosis of T2DM

Embryonal tumors of central nervous system (CNS) are highly invasive malignant tumors that mainly affect infants.Even if the current active treatment strategies are given, the prognosis is still very poor.The 2016 World Health Organization(WHO) classification integrates the genetic information of diseases with histopathology, so as to make the classification and diagnosis of these tumors more accurate.In the new classification, embryonal tumors of CNS include the most common medulloblastoma, and the rare embryonal tumor with multilayered rosettes, C19MC-altered, embryonal tumor with multilayered rosettes, not otherwise specified (NOS), medulloepithelioma, CNS neuroblastoma, CNS ganglioneuroblastoma, CNS embryonal tumor, NOS, atypical teratoid rhabdoid tumor, and CNS embryonal tumor with rhabdoid features.Further study and exploration on the biological nature of these rare brain tumors will provide scientific basis for new treatment methods.

The integrated course centered on organ system has been implemented for several years in Huazhong university of science and technology.Basic medicine courses were divided into three modules.Medical introduction Ⅰ was the first one which integrated nine basic medicine courses.Reform on teaching methods,teaching content,teacher training and experiment teaching were conducted and initial results were achieved.

Objective To explore the significance of the main effect factors of Th17/Treg for children's gastritis with Hp infection.Methods By prospective study method,40 cases of helicobacter pylori infection related gastritis children were collected as observation group from January 2015 to June 2016,40 cases of children with Hp negative were selected in the same period by physical examination as healthy controls.The main effect factors of Th17/Treg of the two groups were compared.Before and after the treatment,the main effect factors of Th17/Treg of observation group were compared.The main effect factors of Th17/Treg had a correlation analysis.Results The serum IL-17,IL-10 and TGF-beta levels of two groups were compared,and the difference was statistically significant (P ＜ 0.05).Before and after 10 days treatment,serum IL-17,IL-10 and TGF-beta levels of the observation group were compared,and the difference was statistically significant (P ＜ 0.05).Before the treatment,the serum IL-17,IL-10 and TGF-beta levels of observation group had a correlation analysis,and IL-17 and IL-10 were negatively correlated (r =0.778).IL-17 and TGF-beta were also negatively correlated(r =0.767).Conclusion In development process of Hp infection related gastritis for children,the related factors of Th17 and Treg cells involved in the immune pathological process.Immune prevention for children's gastritis with Hp infection provides new train of thought.

Objective To study the differences of suicide mortalities of the urban and rural residents in Liaoning Province. Methods The 1999-2003 suicidal data form Liaoning CDC were analyzed. Results Average suicide mortality was 6.28 for city and 18.34 for country per 100 000 persons in Liaoning Province during 1999 to 2003. The rural suicide mortality was higher than urban (?2=1626.74, P

Quality of life(QOL) is a broad-ranging concept,incorporating in a complex way the person's physical health,psychological state,social relationships,and has been widely used as health outcomes.But in postnatal research or health care in China,the application of QOL was restricted due to the deficiency of assessment tool.Quality of life scale includes two categories: general scale(designed for general population) and specific scale(designed for specific population).General scale didn't cover the specific issues introduced by childbirth.Specific scale for postnatal women was lacking until Symon designed an open Mother-Generated Index(MGI).But the MGI had a high requirement for the understanding ability of audiences,and the completion was easily influenced by environment,both restricted the utilization in China.A specific,valid and reliable postnatal QOL scale is needed to develop in China.The aim of this overview is to describe the traits of the postnatal women's health,the meaning of the application of QOL in this period,and the situation analysis of the application in China.