Objective .To prove aristolochic (AA) caused vascular endothelial cells (VEC) injury via intracellular calcium overloa-ding and investigate the mechanism of calcium dobesilate antagonism. Methods Human umbilical vein endothelial cells (HUVEC) were cultured in vitro, and randomly divided into three groups: Control group, AA group, intervention group. Microscope and transmission elec-tron microscopy were used to observe changes of cell morphology and ultrastructure. ELISA method were applied to determine thrombomedu-lin (TM) in cell culture supernatant, fluorescent indicator FLuo-3/AM and intracellular calcium concentration ([Ca2+]. Results TM val-ue and average [Ca2+] i of AA group were significantly higher than that of control group (P < 0.05). Compared with the AA group, when the concentration of calcium dobesilate was 25 μM or 50 μM, TM value and average [Caz +] significantly decreased in intervention group (P < 0.05). Compared with control group, endoplasmic reticulum was pool expansion shaped, and mitochondrial cristae was absent in AA group cells. Endoplasmic reticulum and mitochondria patterns in the intervention group cells showed some improvement, compared with AA group. Conclusion AA induced VEC calcium overloading, 'I'M secretion and injury of endothelial ceils, endoplasmic reticulum and mito-chondria destruction. Dabesilate calcium could protect endoplasmic reticulum and mitochondria and reduce AA induced VEC calcium over-loading, and these could protect VEC.

Objective To explore the expression levels of cholinesterase(CHE)and haptoglo-bin(HPT)in serum of children with recurrent respiratory tract infections(RRTI)and their value in evaluation of prognosis.Methods A total of 112 children with RRTI were selected and included in the RRTI group,and 95 normal children who underwent physical examination during the same period were randomly selected and included in control group.According to the severity of the disease,pa-tients in the RRTI group were divided into mild group of 38 cases,moderate group of 40 cases,and severe group of 34 cases.The levels of serum CHE,HPT,interleukin-1(IL-1),interleukin-6(IL-6),immunoglobulin A(IgA),immunoglobulin M(IgM),as well as CD3+and CD3+CD4+were detected.The correlation between CHE and HPT in RRTI children was analyzed.The predictive value of serum CHE and HPT expression levels on the prognosis of RRTI children was analyzed.Results The CHE expression level in the RRTI group was significantly lower than that in the control group,while the HPT level was significantly higher(P＜0.05).The CHE level in severe RRTI patients was signifi-cantly lower than that in the moderate and mild groups,and the moderate group was significantly lower than the mild group;the HPT level in severe RRTI children was significantly higher than that in the moderate and mild groups,and the moderate group was significantly higher than the mild group(P＜0.05).The CHE expression level in RRTI children was negatively correlated with the HPT ex-pression level(r=-0.637,P＜0.001).The levels of IL-1,IgA,IgM,CD3+and CD3+CD4+,in the RRTI group were significantly lower than those in the control group,while the IL-6 level was significantly higher(P＜0.05).The CHE expression level in RRTI children was positively correla-ted with IL-1,IgA,IgM,CD3+and CD3+CD4+,and negatively correlated with IL-6;the HPT ex-pression level was negatively correlated with IL-1,IgA,IgM,CD3+and CD3+CD4+,and positive-ly correlated with IL-6(P＜0.05).The serum CHE expression level in RRTI children with good prognosis was significantly higher than that in RRTI children with poor prognosis,while the HPT level was significantly lower(P＜0.05).The area under the curve(AUC)of the combined predic-tion of CHE and HPT for the prognosis of RRTI children was higher than that of their individual pre-dictions,and the difference was statistically significant(P＜0.05).Conclusion In the serum of RRTI children,CHE is lowly expressed,while HPT is highly expressed.The combined evaluation of CHE and HPT has high prognostic value for RRTI patients.

Objective To explore the expression levels of cholinesterase(CHE)and haptoglo-bin(HPT)in serum of children with recurrent respiratory tract infections(RRTI)and their value in evaluation of prognosis.Methods A total of 112 children with RRTI were selected and included in the RRTI group,and 95 normal children who underwent physical examination during the same period were randomly selected and included in control group.According to the severity of the disease,pa-tients in the RRTI group were divided into mild group of 38 cases,moderate group of 40 cases,and severe group of 34 cases.The levels of serum CHE,HPT,interleukin-1(IL-1),interleukin-6(IL-6),immunoglobulin A(IgA),immunoglobulin M(IgM),as well as CD3+and CD3+CD4+were detected.The correlation between CHE and HPT in RRTI children was analyzed.The predictive value of serum CHE and HPT expression levels on the prognosis of RRTI children was analyzed.Results The CHE expression level in the RRTI group was significantly lower than that in the control group,while the HPT level was significantly higher(P＜0.05).The CHE level in severe RRTI patients was signifi-cantly lower than that in the moderate and mild groups,and the moderate group was significantly lower than the mild group;the HPT level in severe RRTI children was significantly higher than that in the moderate and mild groups,and the moderate group was significantly higher than the mild group(P＜0.05).The CHE expression level in RRTI children was negatively correlated with the HPT ex-pression level(r=-0.637,P＜0.001).The levels of IL-1,IgA,IgM,CD3+and CD3+CD4+,in the RRTI group were significantly lower than those in the control group,while the IL-6 level was significantly higher(P＜0.05).The CHE expression level in RRTI children was positively correla-ted with IL-1,IgA,IgM,CD3+and CD3+CD4+,and negatively correlated with IL-6;the HPT ex-pression level was negatively correlated with IL-1,IgA,IgM,CD3+and CD3+CD4+,and positive-ly correlated with IL-6(P＜0.05).The serum CHE expression level in RRTI children with good prognosis was significantly higher than that in RRTI children with poor prognosis,while the HPT level was significantly lower(P＜0.05).The area under the curve(AUC)of the combined predic-tion of CHE and HPT for the prognosis of RRTI children was higher than that of their individual pre-dictions,and the difference was statistically significant(P＜0.05).Conclusion In the serum of RRTI children,CHE is lowly expressed,while HPT is highly expressed.The combined evaluation of CHE and HPT has high prognostic value for RRTI patients.

Objective:To retrospectively analyze the treatment status of tyrosine kinase inhibitors (TKI) in newly diagnosed patients with chronic myeloid leukemia (CML) in China.Methods:Data of chronic phase (CP) and accelerated phase (AP) CML patients diagnosed from January 2006 to December 2022 from 77 centers, ≥18 years old, and receiving initial imatinib, nilotinib, dasatinib or flumatinib-therapy within 6 months after diagnosis in China with complete data were retrospectively interrogated. The choice of initial TKI, current TKI medications, treatment switch and reasons, treatment responses and outcomes as well as the variables associated with them were analyzed.Results:6 893 patients in CP ( n=6 453, 93.6%) or AP ( n=440, 6.4%) receiving initial imatinib ( n=4 906, 71.2%), nilotinib ( n=1 157, 16.8%), dasatinib ( n=298, 4.3%) or flumatinib ( n=532, 7.2%) -therapy. With the median follow-up of 43 ( IQR 22-75) months, 1 581 (22.9%) patients switched TKI due to resistance ( n=1 055, 15.3%), intolerance ( n=248, 3.6%), pursuit of better efficacy ( n=168, 2.4%), economic or other reasons ( n=110, 1.6%). The frequency of switching TKI in AP patients was significantly-higher than that in CP patients (44.1% vs 21.5%, P<0.001), and more AP patients switched TKI due to resistance than CP patients (75.3% vs 66.1%, P=0.011). Multi-variable analyses showed that male, lower HGB concentration and ELTS intermediate/high-risk cohort were associated with lower cytogenetic and molecular responses rate and poor outcomes in CP patients; higher WBC count and initial the second-generation TKI treatment, the higher response rates; Ph + ACA at diagnosis, poor PFS. However, Sokal intermediate/high-risk cohort was only significantly-associated with lower CCyR and MMR rates and the poor PFS. Lower HGB concentration and larger spleen size were significantly-associated with the lower cytogenetic and molecular response rates in AP patients; initial the second-generation TKI treatment, the higher treatment response rates; lower PLT count, higher blasts and Ph + ACA, poorer TFS; Ph + ACA, poorer OS. Conclusion:At present, the vast majority of newly-diagnosed CML-CP or AP patients could benefit from TKI treatment in the long term with the good treatment responses and survival outcomes.

Humans ; Aged ; Lung Diseases ; Pneumonia/drug therapy* ; Adrenal Cortex Hormones/therapeutic use* ; Pulmonary Disease, Chronic Obstructive/drug therapy* ; Administration, Inhalation ; Community-Acquired Infections/drug therapy*