Objective:To establish an ICP-MS method to determine the contents, extraction and migration of Mg and Al in poly-propylene infusion bottles. Methods:An ignition pretreatment method was adopted to determine the contents of Mg and Al in the mate-rials. Five different extraction media with extreme extraction were used to extract and test Mg and Al in polypropylene infusion bottles. Three injections commonly used in clinics were put into polypropylene infusion bottles to carry out accelerated experiment, and the con-tents of Mg and Al were determined by ICP-MS. Results:Mg and Al both had a good linear relationship within the range of 0. 025-1. 0μg·ml-1 (r =0.999 8 and 0.999 0). The recoveries for polypropylene infusion bottles and extraction and migration test were 93. 77%-105. 0% and 96. 53%-103. 89% with RSD of 0. 4%-6. 9% and 1. 0%-7. 1%(n=9), respectively. Conclusion:The meth-od is accurate, sensitive and simple, which can provide reference for the safety data of polypropylene infusion bottles.

OBJECTIVEThis study observed the curative effect of sustained negative pressure drainage application on treatment of severe maxillofacial and neck space infection.

METHODSIncision and drainage were performed to treat 18 patients with severe maxillofacial and neck space infection. A small incision was made on the site of the most obvious swelling or fluctuations, and localized negative pressure was applied with a drainage device on the wound during suturing.

RESULTSAmong the 18 patients, 14 were healed, whereas 4 underwent dehiscence of the wound after the operation. Negative pressure was lost as the drainage tubes were removed, and non-negative pressure drainage method was used instead. During the negative pressure treatment, swelling and pain did not increase after the operation. Other complications, such as asphyxia, septic shock, or mediastinal abscess, did not occur. All the patients were healed and eventually discharged from the hospital.

CONCLUSIONSustained negative pressure drainage, which is a modified version of the traditional method of incision and drainage, is an alternative treatment for severe maxillofacial and neck space infection. Such treatment reduces patient pain and eases doctor exertion. Thus, this method provides a new therapeutic strategy for severe maxillofacial and neck space infection.

Objective To study the effect of hyperbaric oxygen (HBO) therapy on changes in the prolifera-tion of endogenous neural stem ceils (NSCs) in neonatal rats after hypoxie ischemic brain damage (HIBD). Meth-ods A total of 150 Sprague-Dawley rats aged 7 d were randomly divided into a normal eontrol group ( CON ), a HIBD group and a HBO treatment group. HBO was administered to the HBO treatment group within 3 h after HIBD at 2 atmospheres, once daily for 7 d. The HIBD model rats were subjected to unilateral carotid artery ligation followed by 2 h of hypoxia (8% O2> ). BrdU/nestin immunofluorescence was used to examine the proliferation of NSCs dynami-cally in the subventricular zone (SVZ) and the dentate gyms (DG) at the 3rd h, 21st h, 3rd d, 7th d and 14th d af-ter HBO therapy. Nestin protein was detected by Western blot analysis at various time points after HIBD. Results In the HIBD rats treated with HBO, proliferation of endogenous NSCs was observed in the SVZ and DG. The prolifer-ating NSCs increased at the 3rd h and 21st h after HBO therapy in the SVZ and DG respectively, peaked at the 7th d after HBO therapy, and decreased by the 14th d after HBO therapy, though their level was still higher than that in the controls. The Western blot analysis showed that nestin protein began to increase at the 21st h after HBO therapy, peaked at the 7th d after HBO therapy, then decreased. Conclusion HBO administered within 3 h after HIBD can promote proliferation of endogenous NSCs in neonatal rats after HIBD.

Objective To test red cells of bone marrow and hematopoietic cells of rats with liver depression and spleen deficiency syndrome in different time period;To discuss the change rules in the blood system. Methods Rat models with liver depression and spleen deficiency syndrome were made by composite method of anger by restraint stress, clipping tails, over fatigue, and abnormal diet. The hemogram, red cells of bone marrow, marrow nucleated red blood cells, stem cell factor, and erythropoietin of male rats in normal group and model group were detected at 2nd, 6th and 9th weeks. Results Compared with normal group, hematokrit and metarubricyte in model group of 2 weeks was higher in the model group (P<0.05);erythropoietin in model group of 6 weeks decreased (P<0.01);red cell counts, red protein level, hemoglobin, hematokrit, and erythropoietin in model group of 9 weeks were lower (P<0.05);red cell counts, hemoglobin, hematokrit, prorubricyte, metarubricyte, and erythropoietin in model group of 6 and 9 weeks were significantly lower than those in model group of 2 weeks (P<0.05, P<0.01). The number of marrow nucleated red blood cells in model group of 2 weeks decreased (P<0.05). Stem cell factor showed no significant difference. Conclusion The rats with liver depression and spleen deficiency syndrome can increase the number of red blood cells in a short time when they suffer from stress stimulation, so that they can fight the damage factors. But long-term stress stimulation will lead the rats to anemia, which may be associated with the reduction of erythropoietin.

Objective To explore the application and problems of using high pressure injection combine detaining needle in cere‐bral thrombosis on CT angiography in rabbit models .Methods 38 rabbit models were divided to experimental group and control group (19 cases each) .Rabbits in the experimental group were injected contrast medium into rabbit ear marginal vein with detaining needles and connected high pressure injection and the control group used intravenous infusion combined high pressure injection . Then observe the CT image quality and infusion outcome in two groups .Results There were 18 cases (94 .74% ) in the experimen‐tal group obtained successful infusion and excellent image quality ,and 13 cases (68 .42% ) in the control group got excellent image , the difference has statistical significance (Z = - 2 .099 ,P = 0 .036) .All of the images of experimental group showed thrombosis clearly .Conclusion High pressure injection combine detaining needle on CT angiography can increase test efficiency ,simplify the operation sequence and achieve evaluating goals .

Objective To prepare a primary dysmenorrhea self-management behavior scale used for female college students in China.Methods Based on Green health education model,a pool of 40 items was made through literature review as well as semistructured interviews on primary dysmenorrhea in female college sudent.In the following,a primary dysmenorrhea scale for female university students with 20 items was made by considering the advice from five obstetrists and gynecologists and employing a pre-test.Then the primary scale was used for an investigation among 100 female college students with dysmenorrheal and the items were screened by using by item analysis,and a scale with 19 items was decided.The revised scale was finalized by using it among 500 female students with dysmenorrheal in a college in Dalian for a formal investigation,with the reliability and validity tested by factor analysis and correlation analysis.Results The finalized scale was estabhshed containing four dimensions of 19 items.The scale internal consistency Cronbach's α coefficient was 0.91,the index on behaviour,gaining knowledge,treatment compliance and asking for help 0.77,0.88,0.86 and 0.84 respectively.The retest reliability was 0.92 and the index on each dimension was 0.76,0.84,0.83 and 0.77,respectively.The total content validity index of scale content validity index (S-CVI) was 0.93.The content validity index of each dimension,the item cotent validity (I-CVI)was 0.93,0.92,0.92 and 0.94,respectively.By exploratory factor analysis and screen plot,four factors were extracted,with a cumulative contribution rate of 62.90％.The correlation coefficients between the various dimensions were 0.12 to 0.35 and the correlation coefficients between the dimensions and total score were 0.49 to 0.78 (all P ＜0.05).Conclusions Primary dysmenorrhea self-management behavior scales for female college students has a good reliability and validity.It can be used as a tool to evaluate the self management behaviors of female college students in our country.

BACKGROUND:5-Fluorouracil occupies an important position in the treatment of gastric cancer, but its long-term use can easily induce adverse reactions such as myelosuppression and leukopenia. Polylactic acid and its copolymers have a higher biocompatibility, and their decomposer cannot gather in the body.
OBJECTIVE:To investigate the in vitro cytotoxicity mechanism of 5-fluorouracil-loaded polylactic acid nanoparticles on gastric cancer cel lines.
METHODS:Ten mice were selected in this study. 5-fluorouracil-loaded polylactic acid nanoparticles (1×10-7, 1×10-6, 1×10-5, 1×10-4 mol/L) were prepared using ultrasonic emulsification method. Kiling effect of polylactic acid nanoparticles on gastric cancer cel lines in vitrowere detected. Then, the inhibition rate was calculated at different concentrations.
RESULTS AND CONCLUSIONS: Under the transmission electron microscope, 5-fluorouracil-loaded polylactic acid nanoparticles had good shape and relatively evenly distributed with no adhesions. After drug administration, the drug concentration was 50% at 24 hours and 62.9% at 72 hours. After 48 hours co-culture with single 5-fluorouracil or 5-fluorouracil-loaded polylactic acid nanoparticles, the viability of gastric cancer cels showed a decrease trend with the increase of drug concentrations, and moreover, 5-fluorouracil-loaded polylactic acid nanoparticles had a better cel inhibition ability than the single 5-fluorouracil (P < 0.05). The IC50value of 5-fluorouracil-loaded polylactic acid nanoparticles was significantly lower than that of 5-fluorouracil (P < 0.05). These findings indicate that polylactic acid nanoparticles as good drug carriers have a strong drug loading capacity and increase drug concentration in the body, but cannot reduce the biological activity of 5-fluorouracil, which provide new ideas for the treatment of gastric cancer.

Objective To explore the relationship among psychological empower,role stressors and job burnout in doctors.Methods According to convenience sampling principle,350 doctors were selected and assessed with the job burnout scale (MBI-GS),the psychological empower scale and role stressors scale.Results The scores of meaning(10.80±3.12) and self-determination (9.33 ± 3.19) were negatively correlated with the scores of job burnout (r value was from-0.28 to-0.47,P＜0.01),the scores of self-efficacy(11.42±2.67)were negatively correlated with the scores of cynicism (1.61 ± 1.21)and reduced personal accomplishment (3.62±1.51)(r=-0.23,-0.51,P＜0.01),and the scores of impact(8.04±3.18) were negatively corrclated with the scores of emotional exhaustion(1.89±1.19) and cynicism(r=-0.17,-0.25,P＜0.01).The scores of role stressors were positively correlated with the scores of emotional exhaustion and cynicism(r=0.15-0.45,P＜0.01),and the scores of role ambiguity(19.09±3.76) were positively correlated with the scores of reduced personal accomplishment (r=0.42,P＜0.01).The self-efficacy and self-determination can negatively predict job burnout(P＜0.01) ;the role overload(15.24±4.62) and role ambiguity can positively predict job burnout(P＜0.01).Conclusion Psychological empower and role stressors are closely related with job burnout,and have significantly predictive effects on job bumout.

This paper was aimed to study the method of rapid determination of total flavonoids in Chrysanthemum of different processing methods by near infrared spectroscopy (NIRS). The Chrysanthemum was dried by three different processes. The three methods were directly drying, drying after steamed and drying after fry, respectively. The determination of total flavonoids in Chrysanthemum by different processing methods was produced by using UV-Vis spectrophotometry. Collecting the NIRS spectra of Chrysanthemum, the quantitative analysis model of total flavonoids content in Chrysanthemum of different processing methods was established by partial least square (PLS) and the model was validated. The correlation coefficient (R2), the root-mean-square error of calibration (RMSEC) and the root-mean-square error of prediction (RMSEP) were 0.996 19, 0.104 and 0.168, respectively. The correlation coefficient of predication (r) was 0.979 3 which state that the prediction was accurate. The method of NIRS had the advantage of fast determination, simple operation and high accuracy of prediction, and could be used for rapid determination of total flavonoids content in Chrysanthemum of different processing methods.

The antagonistic effects of phentolamin(PL) and tolasuline(TS) on norepinephrine(NE)-induced vasoconstriction were investigated in vitro on aortic rings of normotensive and spontaneously hypertensive rats in Kreb-Henseilet solution containing 40?IU/ml insulin.The results showed that in the presence of high level insulin,the antagonistic actions of ?-receptor blocking agents,PL and TS,were markedly decreased.The pharmacodynamic parameters indicated that the affinity index and action intensity of PL and TS were significantly reduced in solution containing high level insulin.The experimental results showed that high concentration insulin could decrease the antagonistic effects of ?-receptor blocking agents on NE-induced vasoconstriction.