Objective: To explore the Asian Resilience Scale’s preliminary revision, reliability and validity in college students of China. Methods: 646 students were investigated with the Asian Resilience Scale and the short form of NEO Five-Factor Inventory. Results: ①Three factors were extracted by exploratory factor analysis and explained 47.1% of variance. ②Confirmatory factor analysis showed good fit between the data and the construct of scale, ?2/df=2.36,RMSEA= 0.055,GFI=0.923,CFI=0.908,NNFI (TLI)=0.894. ③The Cronbach’s ? coefficients, split-half and retest reliability of the total scores were 0.865, 0.836 and 0.894 respectively. ④The total score of the Asian Resilience Scale was significantly negatively correlated with neuroticism and positively correlated with extraversion, openness to experience, agreeableness and conscientiousness. Conclusion: Reliability and validity of the Asian Resilience Scale’s revised Chinese version is corresponded with psychometric standard, and it could be used to assess the resilience of Chinese college students.

Objective To study the change of serum nitric oxide level in patients with type 2 diabetes (2-DM) and in patients with diabetic peripheral neurology (DPN). Methods Nitrose reductase method was used to examine the serum concentration of NO in type 2 diabetics (n=74) and those accompanied with DPN (n=22) and without DPN (n=21). Results ① The serum NO level in the early stage of metabolic disorder of 2-DM patients was higher than that in the control group; it showed a declining trend in the process of DM and was significantly lower in the middle and late stages of DM. ② The 2-DM patients with DPN had significantly lower serum NO level than in those without DPN, but no difference was observed in serum NO level between the diabetics with mild DPN and severe DPN. Conclusion ① Serum NO level in 2-DM patients has a descending changes in the progression of the disease. ② Serum NO level is closely correlated with the occurrence of DPN, but has no obvious association with its progression.

ObjectiveTo investigate the therapeutic effect and function mechanism of ginkgo biloba extract (EGb) for severe acute pancreatitis.MethodsFifty - four patients,who were diagnozed according to the inclusion criteria,were divided into two groups at random.They were from the People's Hospital of Anyang City from November 2006 to December 2010.In treatment group,25 patients were treated with integrated EGb and other comprehensive treatment group,and 21 patients completed the treatment; in the the contrast group,the 29 patients were trested with simple comprehensive treatment group,and 23 patients completed the treatment. Comprehensive therapy included Somatostatin and antibiotic,ect. Besides the comprehensive therapy,patients in the treatment group were intra - venously infused with 87.5 mg EGbeveryday.Before the day of treatment,on the 3rd and 7th day after the treatment,blood TNF-α,IL-6 and amylase level were determined.Blood MDA level was measured and Balthazar CT grades was observed before the day of treatment and on the 7th day after the treatment.Abdominal pain,abdominal distension and tenderness were observed.Pancreatic infection incidence rate was compared between the two groups.All experimental data were processed with SPSS version 11. 0 statistical software. Quantitative data were analyzed byRepeated Measurement ANOVA or t - test.Count data were analyzed by x2 test.ResultsThe values of blood TNF-α,IL-6 and amylase level were significantly lower in treatment group than in contrast group on the 3rd and 7th day after the treatment ( P ＜ 0.05 ).The values of blood MDA and Balthazar CT grades were significantly lower in treatment group than in the contrast group on the 7th day after the treatment (P ＜ 0.05 ).There was a significantly shorter period of abdominal pain,abdominal distension and tenderness in treatment group than in contrast group.( P ＜ 0.01 ).The pancreatic infection incidence rate of the treatment group was lower than that of the contrast group ( P ＜ 0.05 ).ConclusionsEGb could alleviate notably the cytokine release,restrain oxidative stress and remove the free oxygen factor,prevent and treat Pancreatic infection and necrosis.

Humans ; Mycoplasma pneumoniae ; Pneumonia, Mycoplasma ; epidemiology

Objective To observe the effect of 5 fluorouracil (5 FU) on regulating the inflammatory and anti inflammatory cytokines in acute pancreatitis(AP) in rats, and to investigate the mechanism of treatment of AP with 5 FU. Method SD male rats were divided randomly into 3 groups:false operation group (n=6), AP group(n=24) and 5 FU treatment group(n=24). Then, AP group and 5 FU treatment group redivided respectively into 3 subgroups: After the AP model sut up or after 5 Fu treatment 2, 6 and 24 h subgroups (n=8). The animals were killed at the blood samples were taken for measurement of TNF ?, IL 1, IL 6, IL 10 and TGF ?. The wet weight of pancreatic tissue and blood amylase also were observed. Results Both serum inflammatory cytokines (TNF ?,IL 1 and IL 6) and anti inflammatory cytokines (IL 10 and TGF ?) in AP group increased significantly (P

Objective To observe the effects of 5-fluorouracil(5-FU) on prognosis of septic shock in rats.Methods Thirty female Wistar rats was randomly divided into three groups: control group, septic shock group, and 5-FU group. The septic shock model was established by peritoneal injection of 1.8?109cfu/ml E. coli at the volume of 1 ml/100 g weight. The 5-FU group rats were treated intraperitonealy with 5-FU 50 mg/kg weight at 40 minutes after the septic shock model was established. All rats were killed at 6 hours after operation.The mortality, the ascites rate, the lung tissue wet weight and the lung injury score(LIS) were measured.Results The mortality, the ascites rate, the lung tissue wet weight and LIS of the septic shock group was 60%, 90%, (1.55?0.17)g,and(8.00?2.54),respectively. In 5-FU group, these parameters were 0, 10%, (1.36 ? 0.14) g,and(5.20?2.39),respectively. Compared with septic shock group, the mortality and the ascites rate of 5-FU group were significantly decreased, and the lung tissue wet weight and LIS were improved.Conclusion 5-FU can decrease the ascites rate, relieve inflammation and tissue damage.

AIM: To investigate the immunoblot changeS of dystrophin expression in mdx mice after bone marrow stem cells transplantation. METHODS: The experiment was conducted in the laboratory of Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University from September to December 2004. ①Twenty-five mdx mice of 7-8 weeks old were selected and randomly divided into transplantation 4, 8, 12 and 16 weeks groups and blank control group with 5 mice in each group. Meanwhile, 20 C57 mice of 4 to 6 weeks old were selected as donor mice, and 5 C57 mice aged 10 weeks served as positive control mice. ②All mice were preconditioned with 7 Gy ?-ray. After radiotherapy, 2?107 marrow stem cells per mouse (about 0.3-0.5 mL) were injected into the vena caudalis of mice; 0.3 mL PBS was injected into the blank control group and positive control group. ③The mice in 4 transplantation groups were killed at each time point, and the gastrocnemius was harvested to prepare dystrophin samples. The amount of dystrophin expression was detected by Western blot with GAPDH as control. RESULTS: All 25 mdx mice and 5 C57 mice were involved in the result analysis. Western Blot analysis of dystrophin after transplantation: No dystrophin was detected in the blank control group; in the 4 weeks after transplantation group, only few dystrophin expressions were detected, and the dystrophin/GAPDH was about 0.095?0.267; in the 12 weeks after transplantation group, dystrophin/GAPDH was about 0.218?0.338; dystrophin expressions were increased with time, at 16 weeks after transplantation, the expressions were much more than those at 8 weeks (dystrophin/GAPDH: 0.393?0.385, 0.173?0.284, t =6.062, P

Amyotrophic lateral sclerosis is a progressive neurodegenerative disease that targets motor neurons without efficient treatment. Stem cell receives the enormous attention because it from specific tissues can differentiate into motor neuron. Stem cells from specific tissues could be used for amyotrophic lateral sclerosis, such as embryonic stem cells, neural stem cells, bone marrow mesenchymal stem cells, umbilical cord blood-derived stem cells and induced pluripotent stem cells. The mechanism of stem-cell therapy includes cell-replacement, delivery neurotrophic factors and immunomodulation. Animal researches and some clinical trails have confirmed that stem cells have great potential to treat neurodegenerative diseases. Till now, people are still unknown some aspects of stem cells, and many problems still need to be resolved.

This paper explores the social care for HIV-infected people and patients in Shanxi Province,detailed as two parts including medical assistance and living assistance.And the purpose of this paper is to reflect the status of comprehensive prevention and treatment of AIDS in Shanxi Province,and find out the emerging priorities and challenges in work to provide references for relevant research.

The antagonistic effects of phentolamin(PL) and tolasuline(TS) on norepinephrine(NE)-induced vasoconstriction were investigated in vitro on aortic rings of normotensive and spontaneously hypertensive rats in Kreb-Henseilet solution containing 40?IU/ml insulin.The results showed that in the presence of high level insulin,the antagonistic actions of ?-receptor blocking agents,PL and TS,were markedly decreased.The pharmacodynamic parameters indicated that the affinity index and action intensity of PL and TS were significantly reduced in solution containing high level insulin.The experimental results showed that high concentration insulin could decrease the antagonistic effects of ?-receptor blocking agents on NE-induced vasoconstriction.