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Objective To establish an analytical method for fingerprint of Citri Reticulatae decoction pieces by HPLC. Methods Thermo C18 chromatographic column (4.6 mm×250 mm, 5μm) was used with methanol-0.3%phosphoric acid in gradient elution. The flow rate was 0.8 mL/min, the detection wavelength was set at 280 nm, and the temperature was 40 ℃. The similarities of the 15 batches of samples were compared by similarity evaluation, cluster analysis and principal component analysis. Results Based on the fingerprints of Citri Reticulatae decoction pieces, 8 common peaks were identified. The similarities were all more than 0.9 among all batches. 15 batches can be divided into three categories. Conclusion The method is simple and steady. It provides a basis for the quality control and evaluation of Citri Reticulatae decoction pieces.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Periarthritis ; therapy

Objective To explore the accuracy rate of CO2 injection method and conventional magnetic resonance imaging (MRI) in the staging diagnosis for stage Ⅰ carcinoma of endometrium.Methods Preoperative staging diagnosis for stage Ⅰ carcinoma of endometrium was done,and the method of injecting CO2 gas into the uterine lumen by catheter was applied for pelvic MRI scan in 38 cases of stage Ⅰ carcinoma of endometrium.The pathological staging result was treated as the gold standard to compare the accuracy rate of CO2 injection method with conventional scan method (43 cases).Results For conventional MRI scan group,the accuracy rate of staging diagnosis in stage Ⅰ carcinoma of endometrium was 81.3 ％ (35/43),including stage Ⅰ A 75.0 ％(6/8),stage Ⅰ B 83.9 ％(26/31),stage Ⅰ c 75.0 ％(3/4).For CO2 injection group,the accuracy rate was 89.4 ％(34/38),including stage Ⅰ A 85.7 ％(6/7),stage Ⅰ B 88.9 ％(16/18),stage Ⅰ c 84.6 ％ (12/13).There was a statistical difference between the accuracy rates of two methods (x2=7.81,P ＜ 0.05).Conclusion Compared with conventional scan method,the CO2 injection method with better simplicity,safety and application value,could be more accurate to determine the location of endometrial cancer and the degree of myometrial infiltration.

Objective To discuss the protective effect of Tongsaimai pellets(TSMP) and butylphthalide(NBP) on PC12 cell in the hypoxia/hypoglycemia model.Methods Two ischemia models including hypoxia,hypoglycemia models were used to assay the anti-ischemic roles of TSMP and NBP in cultured PC12.Results TSMP and NBP possessed obvious protective effects on PC12 cells from two injured models.Both of them could increase the number of living cells and decrease LDH activity significantly,particularly in hypoglycemia injured model.Conclusion TSM and NBP have protective effect on PC12 cells from two injured models effectively in vitro.

Objective To evaluate the pain control, general condition and well-being of the patients with advanced cancer treated with transdermal fentanyl as well as the effects of transdermal fentanyl on heart rate variability (HRV) and endocrine function. Methods This open prospective study included 40 patients with advanced cancer. The direct conversion ratio from oral morphine to transdermal fentanyl system (TFS) was 100:1 daily. In patients who had never received any opioid before the initial dose of TFS was 25?g/h. The transdermal system was changed every 72h and the dosage was adjusted to the need of the patients according to the VAS scores and the requirement of morphine. 8 hospitalized patients were monitored by 24h Holter for changes in HRV. Blood samples were taken before transdermal fentanyl patch when pain was severe and 24h after TFS for determination of ACTH,?-endorphin, growth hormone(GH) and insulin levels.Results Pain relief during treatment with transdermal fentanyl was significant accoring to VAS scores. Well-being scores showed that patients' quality of life including appetite, sleep, mental state and daily activities was significantly improved after two weeks treatment. The ratio of LF vs HF, which is one of HRV frequency indices, was obviously reduced at 24h after transdermal fentanyl patch as compared with that before TFS (P

OBJECTIVE:To prepare the Compound anisodamine and neostigmine sustained-release tablet and study the in vitro drug release behavior. METHODS:Using raceanisodamine and neostigmine methyl sulfate as main medicines,hydroxypropyl meth-yl cellulose as sustained release skeleton material,magnesium stearate as the lubricant,polyvinyl pyrrolidone as the adhesive,pre-gelatinized starch as the thinner,microcrystalline cellulose as the disintegrant and filler,Compound anisodamine and neostigmine sustained-release tablet was prepared by wet granulation method and direct compression method. The in vitro cumulative release rate within 12 h of the 2 main medicines was detected by HPLC method. RESULTS:Compound anisodamine and neostigmine sus-tained-release tablet was successfully prepared,and the in vitro release was basically completed within 12 h,with accumulative re-lease rate of 91.3% for anisodamine and 96.5% for neostigmine. CONCLUSIONS:Compound anisodamine and neostigmine sus-tained-release tablet that can cumulatively release for 12 h is successfully prepared.

Objective To study the clinical appilication of detachable balloon embolization techenique in carotid cavernous fistula. Methods 27 cases consisted of 26 cases of typical TCCF and 1 case of CCF caused by left anterio cerebral artery(A1) aneurysm ruptured . 27 cases were treated by endovascular embolization with detachable balloon. Transcranial Doppler ultrasound(TCD) was used before and after embolization.All the patients were followed-up for more than 6 months. Results 22 cases of TCCF were cured by detachable embolization and their internal carotid artery(ICA) remained patent. The first segement of left anteriol cerebral artery was embolized by two detachable balloons in the patient with CCF caused by ruptured aneurysm. Internal carotid artery was embolized by two balloons in 4 cases. There was no severe complication in this group. Conclusions Detachable balloon embolization is a safe and effective method for internal carotid cavernous fistula.

The aim was to determine the antimicrobial activities of peptide antibiotics hPAB ? and construct the recombinant baculovirus of its mutants. Four mutants of hPAB ? were designed based on molecular autosyndetic modeling and its genes were cloned by PCR. The transfer plasmid and recombinant baculovirus were constructed. The results showed that the hPAB ? cloned previously, is a good peptide antibiotics. Four mutant genes of hPAB ? were inserted into pFAST HTa plasmid and the recombinant pFAST hPAB ? were screened by restriction enzymes analysis and DNA sequencing. The recombinant baculovirus was obtained after transforming pFAST hPAB ? into Escherichia coli DH10Bac. Our work lays a good foundation for further research.

Hepatitis, Autoimmune ; Humans ; Immunoglobulin G