Supported by Center of Laboratory Medicine of PLA,the Third Military Medical University launched teaching reform on laboratory diagnostics by integrating and optimizing teaching contents,adopting case centered teaching method,introducing thinking modes of evidence-based laboratory medicine and strengthening bilingual teaching.Abilities of students to determine the clinical stage and to evaluate the effect of treatment as well as prognosis of diseases were promoted.

Objective To observe the effect of Huolisu oral liquid combined with mirtazapine tablets on the expressions of NE and 5-HT in the patients with depression,and explore the therapeutic efficiency. Methods 75 patients with depression were randomly divided into a study group with 43 cases,which were treated with Huolisu oral liquid and mirtazapine tablets and a control group with 32 cases,which were only treated with mirtazapine tablets The Hamilton depression scale(HAMD)score, serum NE,and 5-HT were detected in the patients before the treatment and 4 and 8 weeks after the treatment separately. Results Before treatment,there were no statistical significance in HAMD score,serum depression NE and 5-HT(t=0.501, 0.520, 0.611,P>0.05). After treated for 4 and 8 weeks, compare with the control group, HAMD score was decreased(t=1.829、2.263, P<0.05), serum 5-HT was increased(t=2.119, 2.870, P<0.05),and NE was increased in the study group(t=2.264, 2.705,P<0.05). Conclusion Huolisu oral liquid combined with mirtazapine tablets can significantly decrease HAMD score,and increase serum NE and 5-HT level. The treatment can improve the depression symptoms in the patients with depression obviously.

Objective To analyze and discuss the value and significance of coronary artery bypass grafts in the same period of heart valve replacement in the process of the coronary heart disease with heart valve disease treatment, in order to improve the effect of treatment and reduce the incidence of adverse events. Methods 60 cases with coronary heart disease with heart valve disease , which admitted in our hospital during February 2013-February 2015, were selected as the research objectaccording to random number table method and divided into the-experimental group and the control group , each group of 30 cases according to the principle of double blind control. The experimental group was given the coronary artery bypass grafts in the same period of heart valve replacement and the control group was given the coronary artery bypass grafts without heart valve replacement. The curative ef-fects of different schemes and effects on cardiac function after surgery of the two groups were compared. Results After statistical analysis found thatthe observation indexes of the control group was significantly increased compared with the experimental group, the difference was significant statistically significant (P<0.05). The postoperativecar-diac function indexes of the experimental group was significantly improved then control group , the difference was significant statistically significant (P<0.05). Conclusion Coronary heart disease with heart valve disease in the process of treatment can also take heart valve surgery combined coronary artery bypass surgery. By preoperative and postoperative symptomatic treatment, intraoperative protect myocardium, can effectively prevent and reduce the op-eration of various complications, which is worthy of clinical popularization and application.

The integrated course centered on organ system has been implemented for several years in Huazhong university of science and technology.Basic medicine courses were divided into three modules.Medical introduction Ⅰ was the first one which integrated nine basic medicine courses.Reform on teaching methods,teaching content,teacher training and experiment teaching were conducted and initial results were achieved.

Objective To find out the bioactive constituents in the stem of Lonicera japonica and to carry out a systematic study on the chemical constituents of the plant. Methods Three compounds were isolated from the plant by means of solvent extraction and column chromatography, and their structures were elucidated by spectral analyses. Results All these compounds were identified as luteolin (Ⅰ), loga-nin (Ⅱ), r-1-(4-hydroxy-3-methoxy-phenyl)-trans-2, cis-3-dihydroxymethyl-7, 8-dihydroxy-6-methoxy-1, 2, 3, 4-tetrahydro-naphthalene (Ⅲ), respectively. Conclusion Compound Ⅲ is a new compound named as japenol.

Objective: To investigate the relationship between the c-Jun N-terminal kinase (JNK) pathway and lung-resistance protein (LRP). Methods:A549 cells were treated with various concentrations of CDDP for 72 h. The LRP mRNA expression was then analyzed using reverse transcription PCR (RT-PCR). LRP, JNK, and P-JNK were analyzed by Western blotting. The A549 cells were then pretreated with SP600125 (2 μg/ml to 4 μg/ml ) for 1 h. Afterward, CDDP (16 μg/ml) was added into the culture for 72 h. A Cell Counting Kit-8 was used to investigate the sensitivity of CDDP to the A549 cells. Flow cytometry was used to detect the apoptosis rate. The LRP mRNA expression was analyzed using RT-PCR. LRP, JNK, and P-JNK were analyzed by Western blotting. Results:CDDP in-duced the mRNA and protein expression of both LRP and P-JNK in a dose-dependent manner. Pretreatment with SP600125 enhanced the sensitivity of CDDP to the A549 cells and increased the apoptosis rate. However, the LRP mRNA and LRP expression in the pre-treated cells was lower than that in the presence of CDDP alone. Conclusion:In A549 cells, CDDP induces the LRP expression via the JNK pathway. This result suggests that lung cancer therapy can be improved by the addition of CDDP to inhibit the JNK signaling path-way.

BACKGROUND: Retinitis pigmentosa(RP) is a group of commonest genetic blindness-inducing eyeground diseases, which have relative great heterogenicity in both heredity and phenotype. Pierce et al discovered a new retinal photoreceptor cell specific gene-RP1 in 1999, and also found in their following research that the mutation of this gene can induce autosomal dominant RP(adRP) . Present RP1 molecular genetic researches mainly concentrate in Caucasians.OBJECTIVE: To investigate the mutation frequency, characteristics of RP1gene in Chinese RP patients and its role in RP pathogenesis.DESIGN: A comparative study by employing RP patients as subjects and healthy individuals as control.SETTING: Gene diagnosis and therapy center in a hospital affiliated to a military medical university of Chinese PLA.PARTICIPANTS: Totally 101 RP patients without genetic classification were visited patients of the outpatient Department of Ophthalmology of Hong Kong Prince Wales Hospital and Hong Kong Hospital of Ophthalmology between January 1998 and December 2001, which aged between 10 and 79years old(including 43 male and 58 female cases) with an average age of 40years old. Inclusive criteria: Cases who were in accordance with the general national and international standards for RP diagnosis(including funduscope observation and electroretinogram test). Exclusive criteria: patients of other retinal pathological changes. A total of 190 healthy adults were selected in control group, which had no RP family history and no RP or other eye diseases in eye examination, for the confirmation of whether the detected variation was the polymorphism of RP1 gene.METHODS: Totally 101 cases received conformation sensitive electrophoresis(CSGE) and DNA direct sequencing analyses to detect the point mutation in entire RP1 gene encoding range.MAIN OUTCOME MEASURES: Mutation frequency and patterns of RP1gene in Chinese RP patients and its role in RP pathogenesis.RESULTS: The mutation detectable rate of RP1 gene in all PR patients was 1/101. Mutation ultimately caused serious truncation in RP1 protein. The phenotype of the disease might be originated from functional deficiency in PR1protein synthesis. In addition, 10 missense mutations were found in our study population, most of which were RP1 gene polymorphism except the unconfirmed pathological significance of M479I.CONCLUSION: The deletion of corresponding segments(codon 1052-1933) in RP1 protein would induce RP. Large-scale RP1 genotying is necessary, which also can discover more RP-inducing mutation and RP1 gene polymorphism different from other races simultaneously for further fundamental therapy of RP and thorough improvement of the quality of life of the patients.

Distortion product otoacoustic emission (DPOAE) signal can be used for diagnosis of hearing loss so that it has an important clinical value. Continuously using sweeping primaries to measure DPOAE provides an efficient tool to record DPOAE data rapidly when DPOAE is measured in a large frequency range. In this paper, locally weighted least squares estimation (LWLSE) of 2f1-f2 DPOAE is presented based on least-squares-fit (LSF) algorithm, in which DPOAE is evoked by continuously sweeping tones. In our study, we used a weighted error function as the loss function and the weighting matrixes in the local sense to obtain a smaller estimated variance. Firstly, ordinary least squares estimation of the DPOAE parameters was obtained. Then the error vectors were grouped and the different local weighting matrixes were calculated in each group. And finally, the parameters of the DPOAE signal were estimated based on least squares estimation principle using the local weighting matrixes. The simulation results showed that the estimate variance and fluctuation errors were reduced, so the method estimates DPOAE and stimuli more accurately and stably, which facilitates extraction of clearer DPOAE fine structure.
Algorithms ; Hearing Loss ; diagnosis ; Humans ; Least-Squares Analysis ; Otoacoustic Emissions, Spontaneous ; Regression Analysis

Objective To explore the value of multi-slice computed tomography (MSCT) on the typing and staging of bronchiolitis obliterans (BO) in children.Methods Twenty-eight BO patients were recruited and underwent X-ray and MSCT from August 2012 to August 2015.The classification of MSCT signs and radiological manifestations in different stages of BO were discussed.Results Mosaic sign and bronchial wall thickening appeared in all cases and pulmonary atelectasis in 6 patients.MSCT signs were divided into 18 cascs of simple type and 10 of pneumonic type,respectively.Radiological imaging undergone after treatment for 2 ～ 4 weeks indicated that 20 patients turned normal,but 8 patients to chronic stage,including pulmonary interstitial fibrosis (7 cases),bronchoiectasis (6 cases),calcification in bronchial distal (3 cases) and pleural adhesions (2 cases).Conclusion Mosaic sign is a distinctive MSCT manifestation for BO in early phase,and CT typing and staging contribute to guiding the treatment.

Objective To identify the disease locus in X-linked retinitis pigmentosa (RP) families using genetic linkage analysis. Methods Five microsatellite markers were selected from the RP2, RP3, RP6, RP23 and RP24 gene loci, respectively. Haplotype analysis for two X-linked RP families was performed to determine the critical region. Two-point linkage analysis was performed using MLINK program. Results In FYJ and ZCF X-linked RP families, the LOD score was 1.18 and 1.03 at DXS 993, 0.58 and -2.69 at DXS 1068, -2.33 and -2.45 at DXS 1214, -2.34 and -2.51 at DXS 8051, -2.23 and -2.62 at DXS 8043. Conclusion The phenotype of ZCF family is not caused by mutation of RP3, RP6, RP23, RP24 gene, and FYJ family may be linked to RP2 or RP3 gene.