Objective To explore the clinical value of intravenous thrombolysis in the treatment of patients with acute myocardial infarction (AMI) and cardiac arrest after cardiopulmonary resuscitation.Methods 120 patients with AMI and cardiac arrest admitted in our hospital from Mar 2009 to Mar 2013 were divided into thrombolytic group (n=50) and control group (n=70) according to the voluntary principle.The recanalization rate,return time of spontaneous circulation,left ventricular ejection fraction (LVEF) and creatine kinase-MB (CK-MB),complications and hospital mortality rate were compared between two groups.Results The recanalization rate was significantly higher in thrombolytic group than in control group (76％ vs.11.4％,x2 =84.81,P＜ 0.05).Restoration of spontaneous circulation was significantly shorter and LVEF after one week of treatment and CK-MB peak were significantly higher in thrombolytic group than in the control group (t=6.796,4.342,2.923,respectively,P＜0.001 or 0.05).The incidences of heart failure,angina,arrhythmia,and re-infarction were significantly lower in thrombolytic group than in control group (x2 =6.10,20.95,8.52,11.10,respectively,all P＜0.05).There were no significant differences in the incidences of intracranial hemorrhage and upper gastrointestinal bleeding between thrombolytic group and control group (x2 =2.19,3.74,both P＞0.05).Hospital mortality rate was significantly lower in thrombolytic group than in control group (10 ％ vs.25.7 ％,x2 =4.65,P＜0.05).Conclusions Intravenous thrombolysis has a higher recanalization rate,faster recovery time of spontaneous circulation,fewer complications and higher hospital survival rate for the treatment of cardiac arrest in patients with acute myocardial infarction after cardiopulmonary resuscitation.

Sun Zhong Shan got the west medical education and had medical practice.He once put forward his deep medical thought which had great effect on Xin Hai Revolution and even promoted the propagation of the west medicine in china after the Xin Hai revolution.

Objective_To investigate whether overexpression of TRAF4 in human breast cancer may have impact on its cell proliferation.Methods_This study has two groups, MDA-MB-231 transfected with pcDNA3-TRAF4-DM-TRAF or pcDNA3 .We detected the expression and localization of TRAF4 with immunofluorescence and western blot.We detected the expression of the phosphorylation level of p70S6K and S6 with westernblot.Flow cytometry was used to detecte cell cycle.MTT Assay was used to detected cell reproductive capacity.Results_TRAF4 local-ized in both cytoplasm and nuclei in MDA-MB-231.Nuclear expression of TRAF4 in nuclei was lower than that in cytoplasm (P<0.05).After pcDNA3-TRAF4-DM-TRAF was transfected into the cells, the expression of TRAF4 in nuclei was increased (P<0.05).The phosphorylation level of p70S6K and S6 significantly increaseced (P<0.05,P<0.01).More S phase cells were recorded(P<0.01) by FCM.The cell proliferation was promoted by MTT ( P<0.01) .Conclusions_The expression of TRAF4 in nuclei may play an important role in the cell prolif-eratuion by promoting p70S6K and S6 activation.

Objective:To study the influence of gonadal hormones on the function of lymphocytes of SLE patients through detecting the expression of estrogen receptor alpha(ER?) and the function of peripheral blood lymphocytes of SLE patients.Methods:Blood samples of 33 SLE patients and 31 normal controls were collected.Estradiol(E2) and testosterone (T) concentration in peripheral blood were detected by ELISA,the proliferating reaction of T lymphocyte stimulated by ConA were detected by methyl thiazolyl tetrazolium (MTT) assay and the expression of estrogen receptor alpha(ER?)mRNA of peripheral blood mononuclear cells (PBMCs),T lymphocytes and B lymphocytes were detected by RT-PCR.Results:Compared with normal control,the stimulating index of T lymphocyte proliferation decreased(P

Supported by Center of Laboratory Medicine of PLA,the Third Military Medical University launched teaching reform on laboratory diagnostics by integrating and optimizing teaching contents,adopting case centered teaching method,introducing thinking modes of evidence-based laboratory medicine and strengthening bilingual teaching.Abilities of students to determine the clinical stage and to evaluate the effect of treatment as well as prognosis of diseases were promoted.

Objective To explore a simple, effective and rapid method for indoor air disinfection. Methods Three kinds of disinfection methods were carried out (method A: wiping surfaces of floor and walls of room with water and ultraviolet irradiation, method B: wiping surfaces of floor and walls with chloro-disinfectant and ultraviolet irradiation, method C: wiping surfaces with chloro-disinfectant and spraying chloro disinfectant containing chlorine) and the effectiveness of different disinfection methods was compared. Results The result showed that all of the three methods could decrease natural fallen bacteria count in isolated room air to up to the standard, and the disinfection effectiveness of method C was better than the method A and method B. Conclusion The result indicated that method C was an effective and simple method for indoor air disinfection.

Objective To investigate and evaluate the improvement of the quality of life of pulmonary infection in AIDS patients treated by Chinese traditional treatment combined Western medicines treatment.Methods Sixty cases with HIV infection combined with pulmonary infection were selected as our subjects.They were randomly divided into two groups and each group was 30 cases.Patients in western medicine group were given medicine plus placebo,and in Chinese medicine group were given west medicine combined with traditional Chinese medicine.Patients hospitalized for 4 weeks.WHO QOL-HIV plus AIDS-related quality of life scale were used to collect the information about quality of life.Results There were no significant difference between two groups in terms of quality of life,life activity,health status,sleep status,ability to work,leisure opportunities scores at before treatment (P ＞ 0.05).After treatment,the score of quality of life,life activity,health status,sleep status,ability to work,leisure opportunities in patients of Chinese group were (3.78 ± 0.69),(3.55 ± 0.67),(3.55 ± 0.67),(3.18 ± 0.72),(3.66 ± 0.70),(3.03 ± 0.73) respectively,higher than that of western medicine group ((2.90 ± 0.77),(2.75 ± 0.85),(2.75 ± 0.85),(2.85 ± 0.68),(3.35 ± 0.57),(2.95 ± 0.85) respectively,t =-2.791,-2.791,-4.28,-3.08,-4.08,-2.56,P ＜ 0.05).There were no significant differences between the two groups in the terms of the score of satisfaction,sex ability,HIV impaction on the body,the meaning of life,the energy aspects of patients at before treatment (P ＞ 0.05),but after treatment,these scores in Chinese medicine group were higher that in the control group ((3.51 ± 0.67) vs.(2.93 ±0.71),(3.13±0.46) vs.(2.86±0.74),(3.96±0.71) vs.(3.45 ±0.98),(3.66±0.72) vs.(3.23 ± 0.88),(3.23 ± 0.67) vs.(2.90 ± 0.65)),and the differences were statistically significant (t =-4.38,-2.34,-3.75,-2.76,-2.38,P ＜ 0.05).There was no significant difference in the term of the mental effect of HIV within groups at before treatment,but after treatment,the score in Chinese group was lower than the Western group ((3.05 ± 0.90) vs.(3.46 ± 0.87),t =2.31,P ＜ 0.05).Conclusion Chinese medicine combined western medicine can significant improve the quality of life of HIV patients with pulmonary infection.

ObjectiveTo investigate the diagnosis and treatment of gastric paralysis after gastric operation.MethodsThe clinical data of 21 patients with gastric paralysis after gastric operation were reviewed retrospectively.Results3.9% of patients occurred gastric paralysis in this group. The diagnosis was depended on patient's symptoms, X-ray graph of the upper digestive tract and gastroscopy. The patients were healed after conservative treatment.ConclusionThe combined conservative treatment is the ideal method to treat the gastric paralysis syndrome.

Objective To compare the effect of different cryoprotectants and different concentrations on controlled?rate freezing of human ovarian tissues. Methods Ovarian tissues were sampled from 15 patients undergoing benign ovarian tumor surgery. Cortical slices were frozen by controlled?rate freezing using three cryoprotectants,propanediol,ethanediol,and dimethylsulphoxide,and the concentration of each cryoprotectant was 1.5 mol/L or 2.0 mol/L. Cortical slices obtained from each patient were processed with each cryopreservation procedure simultaneously. Morphology of fol?licles was studied by light and electron microscopy and the normal rate was compared with that of the fresh tissues from the patient. Results There were no significant differences in distribution of follicles of different developmental stages between each group(P<0.05). Light microscopy showed 1.5 mol/L EG and 2.0 mol/L EG groups had the best freezing effect,and the difference in the morphologically normal rate of follicles was not statisti?cally significant compared to fresh controls(P>0.05). However,the difference was statistically significant for 1.5 mol/L PROH,2.0 mol/L PROH, 1.5 mol/L DMSO and 2.0 mol/L DMSO groups(P<0.05). Electron microscopy showed the oocyte quality declined after controlled?rate freezing pro?cedure. However,the statistical analysis was not conducted due to little data. The viability of granulosa cells was significantly declined after all the freezing procedures compared to that of the fresh control tissues(P<0.01). The number of morphologically normal granulosa cells was slightly higher in the tissues which had been cryopreserved with 2.0 mol/L PROH and 1.5 mol/L EG,but no significant differences were found between any of two frozen groups(P>0.05). Conclusion Controlled?rate freezing using 1.5 mol/L EG as the cryoprotectant can better save oocytes and granulosa cells. It is a preferable freezing procedure for ovarian tissues.