Pancreatic cancer is a kind of high degree of malignant gastrointestinal tumor,and its incidence has increased significantly in recent years.Due to the low early diagnosis rate,and the extreme low middile or late resection rate of pancreatic cancer,Pancreatic cancer's prognosis is poor.Therefore,improving the early diagnostic rate of pancreatic cancer has very important significance for improving the prognosis.Early diagnosis of pancreatic cancer is dependent on tumor markers detection to a great extent.And proteomics progress rapidly in recent years and has been widely applied in clinical research.It provides an effective way to find markers.Among all of the spec imens,secrum is easy to obtain,and it allows repetitive detection.What is more,secmm is also renewable.So it is the most ideal for biological specimens.In this review,the proteomics technology role in pancreatic cancer patients serum detection were summarized.

OBJECTIVE:To analyze the general patterns and characteristics of drug-induced lupus (DIL) induced by inflix-imab,and to provide reference for the safety of drug use. METHODS:Usinginfiximab,lupusas searching words,inflix-imab-induced DIL literatures included in CJFD,VIP and PubMed from Jan. 2002 to Jun. 2016 were retrieved and analyzed. RE-SULTS:Totally 30 effective literatures were retrieved,involving 31 DIL patients,there were 8 male cases (25.81%) and 23 fe-male cases(74.19%);17 patients aged more than 50(54.84%). 20 cases occurred within 5-24 weeks(64.51%). 16 patients re-ceived infliximab alone (51.61%). Main clinical manifestations were skin rash or photosensitivie enteritig (15 cases),joint pain (16 cases,multiple arthritis and synovitis)and fever(12 cases). 5 patients suffered from 3 above symptoms simultaneously. Labo-ratory examination mainly manifested as antinuclear antibody,double stranded DNA antibody and anti-histone antibody positive. Thirty patients with DIL received drug withdrawal or steroid hormones treatment,among which the symptoms of 26 patients disap-peared after drug withdrawal or treatment within 6 months,and 1 patient deteriorated. CONCLUSIONS:DIL-induced by inflix-imab invovle patients'age and gender,disease types,family history and other factors. Clinicians should be aware of rules and char-acteristics of DIL induced by infliximab,and tighten drug use monitoring to reduce ADR.

Objective To study the quality control of yinpian of Isatis Root.Methods Optical microscope was used to identify the microscopic features,TLC was adopted to identify the arginine in Isatis Root,and HPIC-ELSD was used to detect the quality of arginine.Results and Conclusion There is no difference between yinpian and crude powder of Isatis Root.Arginine can be detected bv TLC.The moisture contents.the total ash contents and the acid-insoluble ash contents in the yinpian should be less than 9.0%,10.0%,and 2.0% respectively;while the alcohol-soluble extractive contents and the contents of arginine in Isatis Root should be more than 25.0% and 2.01% respectively.

Estrogen is involved in the process of postmenopausal osteoporosis, and the main functional cells are osteoclasts. However, the mechanism of estrogen on the osteoclasts is not yet fully understood. Here we demonstrate the effects of estrogen on osteoclasts in three aspects: morphology and structure, apoptosis of osteoclasts and differentiation of preosteoclasts, which give an overall explanation for effects of estrogen on osteoclasts.

ObjectiveTo investigate the changes of serum growth hormone (GH)/insulin-like growth factor- Ⅰ ( IGF- Ⅰ ) in patients with traumatic long bone fractures and the related clinical significance.MethodsTwenty-one patients with traumatic long bone fractures from the same trauma care center treated between November 2009 and April 2010 were enrolled in the study..There were 14 males and 7 females,at age range of 22-60 years (mean 45.0 years).Seventeen patients (81％) were with closed trauma and four ( 19％ ) with open trauma.Enzyme Linked Immunoadsorbent Assay (ELISA) was used to determine the levels of serum GH and IGF- Ⅰ within 14 days after fractures and the relationship between changes of GH-IGF- Ⅰ axis and injury severity score (ISS) was further analyzed.Eight patients with normal GH/IGF- Ⅰ were set as control group.ResultsThe GH level in the peripheral blood was significantly higher at day 1 after fractures ( P ＜ 0.05 ),and rapidly declined and stabilized at day 5 compared with the control group.The GH level in the patients with ISS≥ 18 points was sigmficantly higher than that in those with ISS＜18 points at day 1 after fractures (P＜0.05).The IGF- Ⅰ level in the peripheral blood in the patients with 1SS≥ 18 points was significantly higher than that in those with ISS ＜ 18 points at days 5-10 after fractures ( P ＜ 0.05 ).ConclusionThe hypothalamus GH-IGF- Ⅰ axis plays an important role in early metabolism and fracture healing during the acute phase of traumatic long bone fractures.

Objective To develop a kind of medical rescue vest which is suitable to single pawn in emergency mission. Methods According to the rescue mission of single pawn and medical soldier in the early stage, the quantity and species of rescue equipment in medical rescue vest are studied to meet the medical needs of rescue mission; the determine ways of recovery effect are researched to ensure the adaptation of personnel and equipment; the vest structure is designed by people somatotyoe, the principle of human work efficiency and combination of disaster field characteristics. Results In the course of rescue drilling and rescue practice, the rescue vest is convenient and practical and meets the technology needs of war injury cure such as hemostasis, bandaging, fixing, handling, ventilation and basic life supporting etc. Conclusion The lightweight and practical medical rescue equipments are provided for single pawn and health soldiers to improve the ability of complete rescue mission.

Objective To investigate the status of metabolic syndrome(MS),chronic metabolic disease and insulin resistance(IR) in dwellers over twenty years old in Wulian County,Shandong province.Method 2 127 dwellers over twenty years old were investigated in three towns with multistage stratified sampling and cross sectional methods.Questionnaire was delivered to all the subjects,while physical examination and biochemical test were carried out.Result The prevalent rate of chronic metabolic diseases in dwellers over twenty years old in Wulian County was 64.34%.The prevalent rate of overweight/obesity,diabetes,hypertension and hyperlipemia were 36.24%,3.24%,38.98% and 31.26,respectively.The prevalence of MS having more than three above diseases was 7.9%.The prevalent rate of IR in obesity,diabetes,hypertension,dyslipidemia and MS patients were 43.34%,78.26%,29.79%,33.23% and 64.28%,respectively.Conclusion Near two-thirds of the dwellers over twenty years old in Wulian suffered chronic metabolic disease.IR was common in MS and chronic metabolic disease,especially in diabetes and MS.

Venous thromboembolism (VTE) is a kind of venous disorder that the abnormal condensation of blood within the vein,leading to complete or incomplete vein obstruction.VTE is mainly a combination of deep vein thrombosis (DVT)and pulmonary embolism (PE) and is an important complication after major orthopedic surgery (total hip arthroplasty,total knee arthroplasty,hip fracture surgery).Foreign literature reported that incidence rate of VTE after arthroplasty was as high as 42％-57％.Conventional notion was that DVT and PE were two different but interrelated types of VTE,while the latest research reported that the formation of DVT and PE after arthroplasty may be two events individually.Prevention of VTE after major orthopedic surgery includes general,pharmacological and mechanical methods.The efficacy of physical combined with pharmacological methods was better than drug prevention alone in preventing distal DVT.Dabigatran,apixaban,rivaroxaban and other new oral anticoagulants were better than low molecular weight heparin and other traditional medicines in effectiveness and safety.The effects of low molecular weight heparin are remarkable,while it cannot take orally.A recent study found that staged VTE prophylaxis,namely patients received low molecular weight heparin injection in hospital,and received aspirin,rivaroxaban or dabigatran orally after discharge is safe and effective in prevention of VTE.

BACKGROUND:With the development of biochemistry and cel biology, fracture has being study deeper, blood supply has been known to be an important factor influencing the fracture healing. Endothelial progenitor cel s with good ability of angiogenesis wil have a good clinical prospect in fracture healing.
OBJECTIVE:To review the recent research of endothelial progenitor cel s in fracture healing.
METHODS:A computer-based online search of CNKI, Wanfang, PubMed databases was performed to col ect articles published between 1980 and 2014 with the key words“endothelial progenitor cel , fracture, neovascularization, angiogenesis”in Chinese and English. A total of 48 articles addressing endothelial progenitor cel for angiogenesis in fracture healing were included in result analysis.
RESULTS AND CONCLUSION:Increasing evidence has shown that endothelial progenitor cel s have great ability of neovascularzition and angiogenesis. Endothelial progenitor cel s used in tissue engineering scaffolds can promote the survival rate of scaffolds in vivo, which is appropriate to a great part of delayed union and nonunion patients. However, the large-scale treatment with endothelial progenitor cel s stil has many problems, such as isolation, culture and amplification of endothelial progenitor cel s in vitro, the number of transplanted cel s and selection of scaffolds for transplanted cel s, which need further research.

Applying cellular transplant methods in the treatment of peripheral nerve injury has become a new hot spot in tissue engineering and drawn much attention. Bone marrow stromal cells (BMSCs) grow fast and can be easily purified in vitro. Besides, they have the capability of differentiating into Schwann cells under specific conditions. For all the reasons above, BMSCs are considered good succedaneum of Schwann cells and new seed cells for tissue engineered peripheral nerve. In this review, the physiological functions and bionomics of Schwann cells and BMSCs are introduced, as well as various methods inducing BMSCs into nerve tissue cells and the foreground of the research.