AIM:In order to ensure the quality stability of Fructus Polygoni Orientalis,to study the determination method of the fingerprint of Fruetus Polygoni Orientalis and to establish the fingerprint of Fructus Polygoni Orientalis.METHODS:The HPLC assay was used to establish the fingerprint of Fructus Polygoni Orienlalis and 28 pieces of goods were compared.RESULTS:The fingerprint of Fructus Polygoni Orientalis with 7 common peaks was established.The relative retention time and the ranges of relative area of the common peaks were determined.CONCLUSION:The established fingerprint can be used for the quality control of Fructus Polygoni Orientalis.

Objective: To explore the expression charateristics of long non coding RNA bladder cancer associated transcript-1 (BLACAT1) in the cancer tissue and cancer cells in the patients with non small cell lung cancer (NSCLC) and the regulation mechanism, and to elucidate the effect and clinical significance of BLACAT1 in the occurrence and development of NSCLC. Methods: Gene Expression Omnibus (GEO) database was used to analyze the expression characteristics of BLACAT1 in the NSCLC tissue. Kmplot website was used to analyze the correlations between the expression of BLACAT1 and the survival and prognosis of the NSCLC patients. Real time quantitative PCR (qRT PCR) method was applied to detect the expressions of BLACAT1 in cancer tissue and corresponding adjacent normal tissue and NSCLC cell lines of the NSCLC patients. The specific small interfering RNA for BLACAT1 (si BLACAT1 group) or negative control sequence C si NC group) were transfected into the A549 cells. The mRNA expression level of BLACAT1 in A549 cells in various groups were detected by qRT PCR method; the percentages of A549 cells in different cell cycles were detected by flow cytometry; the clone formation abilities of A549 cells in various groups were detected by cell clone formation experiment. The proliferation activities of cells in various groups were detected by CCK8 assay. qRT PCR and Western blotting methods were used to detect the expression level of CyclinDl and CDKN2B mRNA and protein in the A549 cells in various groups. Results; The results of GSE18842 and GSE19804 in GEO datatase. qRT PCR and Kmplot analysis showed that the expression level of BLACAT1 in NSCLC tissue was significantly increased compared with adjacent normal lung tissue C P< 0.05); the survival time in the patients with low expression of BLACAT1 in cancer tissue was significantly longer than that in the patients with high expression of BLACAT1 ( P= 0. 011). Compared with si NC group, the BLACAT1 expression level in the A549 cells in si BLACAT1 group was significantly decreased ( P< 0. 05). Compared with si NC group, the percentage of A459 cells in Gi phase in si BLACAT1 group was increased C P<0. 05) . and the percentage of A549 cells in S phase was decreased ( P<0. 05); the cell clone formation ability and the cell proliferation activity were decreased ( P<-0. 05 or P<0.01). Compared with si NC group, the expression levels of CyclinDl mRNA and protein in the A549 cells in si BLACAT1 group were significantly decreased C P<0. 05). and the expression levels of CDKN2B mRNA and protein were significantly increased C P< 0.05). Conclusion: LncRNA BLACAT1 is up regulated in cancer tissue and cancer cells of the NSCLC patients, and down regulation of BLACAT1 expression can inhibit the proliferation of A549 cells via modulating the CyclinDl/CDKN2B axis, which may serve as a potential therapeutic target for the NSCLC patients.

Objective To investigate the protective effect of meglumine adenosine cyclosphosp (MAC) on the cerebral ischemia-reperfusion (I/R) injury in rabbits.Methods Twenty four healthy rabbits were randomly divided into control group (n =6),I/R group (n =6),MAC pretreated group (n =6),and MAC treated group (n =6).Cerebral ischemia-reperfusion injury model was made by separating and electrocoagulating vertebral arteries and clipping common carotid arteries in the latter 3 groups after anesthesia.The sham-operated group underwent vessel separation without clipping.L/R group was administered with nothing,while MAC pretreated group with MAC before ischemia,and MAC treated group with MAC just after ischemia.Blood was gathered from jugular vein before ischemia,and 30 min,1 h,and 2 h after reperfusion for testing IL-8,superoxide dismutase (SOD) and malondialdehyde (MDA).The brain tissue slice was observed by optical microscope.Results Compared to control group and before ischemia,the levels of IL-8 and SOD in serum were significantly increased and decreased,and the levels of MDA was significantly increased at 30 min after reperfusion in I/R group; the levels of IL-8 and MDA in serum were significantly increased,and the levels of SOD in serum was significantly decreased at 1 h and 2 h after reperfusion in I/R group.The levels of IL-8 in serum was less at 30 min and 1 h and 2 h after reperfusion in MAC pretreated group than in I/R group.At 1 h and 2 h after reperfusion,the levels of MDA in serum was less and the levels of SOD in serum was higher in MAC pretreated group than in I/R group.At 1 h and 2 h after reperfusion,the levels of IL-8 in serum were less and the levels of SOD in serum were higher in MAC treated group than in I/R group.The levels of MDA in serum were less at 2 h after reperfusion in MAC treated group than in I/R group.Compared to I/R group,pathological change was lighter in the MAC pretreated and MAC treated group.Conclusions MAC has a fine cerebral-protective effect and has no side effect.

Objective:To preliminarily observe the clinical efficacy of microwave hyperthermia combined with intensity-modulated radiotherapy (IMRT) and chemotherapy for patients with locally advanced gastric cancer.Methods:Forty patients who could not been operated or refused operation were enrolled in this clinical trial, who were confirmed as locally advanced proximal or distal gastric cancer by gastroscopy pathology and imaging. Radiotherapy was delivered by IMRT technology for 5 times per week with a total dose of 46 to 56 Gy (median dose of 50 Gy) in 25 to 28 fractions. Synchronous hyperthermia was given at 42 to 44℃ twice a week, 45 min/time. S-1 or capecitabine-based synchronous chemotherapy was performed, d1-14/3 weeks. The symptom remission rate, adverse reactions, objective remission rate (complete and partial remission) and survival were observed.Results:A total of 40 patients, aged between 56 and 83 years (median age of 71 years), were enrolled in this study. The male-to-female ratio was 7: 1. Among them, 38 cases (95%) showed symptom remission. The most common adverse reactions were grade 1-2 gastrointestinal reactions and leukopenia. The objective remission rate was 87.5%, the 2-year progression-free survival and overall survival rates were 68.6% and 70.5%, respectively.Conclusion:Preliminary findings demonstrate that microwave hyperthermia combined with chemoradiotherapy achieve satisfactory outcomes and yield tolerable toxicity in patients with locally advanced gastric cancer.

Objective To study the relationship between exon10 region mutation of adenosine triphosphate-binding cassette transporters A3 (ABCA3) gene and neonatal respiratory distress syndrome (RDS).Method From September 2014 to January 2018,neonates admitted to the neonatal intensive care unit of our hospital were studied.Mongolian and Han Chinese newborns with RDS were assigned into Mongolian RDS group and Han RDS group.Mongolian and Han Chinese newborns without RDS were assigned into Mongolian control group and Han control group.The genotype frequencies and allele frequencies of ABCA3 exon10 in each group were compared.Result A total of 320 cases were studied,including 60 cases in Mongolian RDS group,100 cases in Han RDS group,60 cases in Mongolian control group,and 100 cases in Han control group.A single-base point mutation C ＞ T in the ABCA3 rs13332514 (F353F) locus was founded in both Mongolian and Han Chinese newborns.The mutation rates in Mongolian RDS group and Mongolian control group were 26.7％ and 18.3％,and the difference was statistically significant (x2 =6.316,P =0.043);the mutation rates in Han RDS group and Han control group were 21.0％ and 13.0％,the difference was also statistically significant (x2 =7.426,P =0.009).No significant differences existed between Mongolian RDS group and Han RDS group,and between Mongolian control group and Han control group (P ＞ 0.05).The genotypes of CC,CT and TT were detected in both Mongolian and Han RDS groups,and the CC and CT genotypes were also detected in the control group.The T allele frequency was 18.3％ in Mongolian RDS group,and 9.2％ in Mongolian control group.The difference was statistically significant (x2 =4.251,P =0.039).The T allele frequency in Han RDS group was 15.0％,and 6.5％ in Han control group.The difference was also statistically significant (x2 =7.530,P =0.006).Conclusion A single-base point mutation C ＞T in the rs13332514 (F353F) locus of ABCA3 exonl0 may be related to the occurrence of RDS in Mongolian and Han newborns in Inner Mongolia.Allele T may be one of the susceptibility genes of RDS.

OBJECTIVE： To analyze chemical components as coumarin in Saposhnikovia divaricata， and to provide reference for comprehensive analysis of pharmacodynamic material base in S. divaricata. METHODS： UPLC-Q-TOF-MS method was adopted. Chromatographic condition： the determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1％ formic acid water-0.1％ formic acid acetonitrile （gradient elution） at the flow rate of 0.3 mL/min. The column temperature was 35 ℃， and sample size was 3 μL. MS condition： ESI， in positive and negative ion mode， ESI+/ESI－ 5 500 V/－4 500 V， declustering potential of 80-－80 V， auxiliary heating gas pressure of 55.00 psi， atomizing gas pressure of －55 psi， curtain gas pressure of －35.00 psi， desolvent temperature of 550 ℃， collision activation scanning energy of 15 eV， collision voltage of 35 psi. Component analysis was performed by comparing with the related literature data and the standard chromatogram control combined with accurate relative molecular mass of compounds provided by UPLC-Q-TOF-MS. RESULTS： Totally 135 chemical components were analyzed in ESI+ mode， and the 105 chemical components were analyzed in ESI－ mode. 11 chemical components as coumarin were identified in ESI+ mode， such as isoimperatorin， umbelliferone， scopolamine， xanthotoxin， psoralen， Ostenol， fraxidin， isoimperatorin， 5-hydroxyl-8-methoxypsoralen， phellopterin， decursin. CONCLUSIONS： The method is accurate and rapid， and can be used for the analysis of chemical components as coumarin in S. divaricata.