The Socialist legal construction needs to strengthen the legal education.It is significant to seek for the path of legal education in order to enhance the actual effect of legal education.The path of legal cducation includes traditional education,utilitarian education,reasonable education,legitimate education and feeling education,etc.We can get a better actual effect of legal education if different paths of legal education (traditional education,utilitarian education,reasonable education,legitimate education and feeling education) can be integrated,infiltrated and complemented.

The tumorigenesis of breast cancer is a multistep process with many factors.The microRNAs (miRNA) participates in the development and distance metastasis of tumor by regulating proliferation,apoptosis and migration of tumor cells.The study of the mechanisms that miRNA impacting breast cancer cell proliferation and metastasis may provide new ideas for the diagnosis and treatment of breast cancer.

Objective To explore the influences of indoor and outdoor sources of air pollutants on NO 2 concentra tions in indoor air.Methods The dynamic variations of NO 2 concentrations in indoor and outdoor air were continuously monitered by ML 9841B chemiluminescence continuous monitoring analyzer in2families of White haven town of west Cumbrra,UK.Results During the period of indoor air monitoring,typical background concentrations of NO 2 in air in the center of White haven were between19and28?g /m 3 (10min-mean),and rose to38-47?g /m 3 (10min-mean)during the morning and evening rush of traffic.NO 2 concentration increased about 190?g /m 3 (10min-mean)after the use of a gas cooker in the kitchen A1-hour mean concentration of253?g /m 3 was recorded in the kitchen air of one of the houses,which substantially exceeded the NAQS standard of200?g /m 3 .Conclusion The increase of NO 2 concentration in kitchen air induced by the use of gas appliances played a more important role in influencing the indoor air quality than outdoor air did.

Objective To investigate the gene polymorphism of HLA-DR locus and feature of HLA haplotypes of Han Population in Tianjin area.Methods Reverse PCR-SSOP typing results of HLA-DR alleles of 3000 cord blood units from Han population pregnant women in Tianjin area were compared to those of European Caucasian and the Han populations in Xi’an area.Results DRB1 *15, allele presented the highest frequency,followed by DRB1 *09,DRB1 *07,DRB1 *12,and DRB1 *04.The main haplotypes were A *02-DRB1 *09,A *02-DRB1 *15,B *13-DRB1 *07,A *02-DRB1 *12,A *30-DRB1 *07 and B *46-DRB1 *09 in sequence.The frequencies of DRB1 *12 and DRB1 *09 alleles were significantly higher than in European Caucasian,while the frequencies of DRB1*01 and DRB1 *03 alleles were significantly lower than those in European Caucasian.As compared with that of the Han population in Xi’an,there was not significantly difference except a decrease in frequencies of DRB1 *04 and DRB1 *13 alleles.Conclusions Distribution of the allelic polymorphism of HLA-DR locus was similar to those in northern Han population,such as in Xi’an area,and it was obviously different from those in European Caucasian.

Oxidative damage theory is currently one of the predominant theories on the mechanisms of aging. Previous research has shown that antioxidants can extend the lifespan in the model organism by scavenging free radicals,inducing the expression of stress related genes and hormesis. However, recent studies have suggested that these pharmaceuticals may cause serious side effects,such as promoting oxidation,increasing the risk of cancer,and destroying the metabolic balance. The low absorption and targeting property also limit the efficiency of most antioxidants. As a result ,the correlation between antioxidants and lifespan extension remains to be demonstrated. We reviewed the research progress in the field of lifespan extension by antioxidants in recent years and provided references for future research in related areas.

Objective: To investigate the prevalence of depression and anxiety among transgender populations and the correlation with defense mechanism, so as to provide the evidence for improving mental health among transgender populations. Methods: Transgender populations that visited Psychosexual Outpatient Department of Beijing Huilongguan Hospital for the first time from December 2020 to December 2021 were enrolled. Participants' demographics, depression, anxiety and type of defense mechanisms were collected using self-designed questionnaires, Self-rating Depression Scale, Self-rating Anxiety Scale and Defense Style Questionnaire (DSQ). Factors affecting depression and anxiety were identified using a multivariable logistic regression model. Results: Totally 126 transgender individuals were enrolled, including 95 men (75.40%) with a mean age of (21.53±4.55) years and 31 women (24.60%) with a mean age of (23.58±5.55) years. The prevalence of depression was 46.83% among participants, including 44.07% of participants with mild depression, 30.51% with moderate depression and 25.42% with severe depression, and the prevalence of anxiety was 26.19% among participants, including 60.61% of participants with mild anxiety, 21.21% with moderate anxiety and 18.18% with severe anxiety. The detection of depression was 54.74% among men and 22.58% among women (P<0.05), and the detection of both depression (62.79% vs. 38.55%, P<0.05) and anxiety (41.86% vs. 18.07%, P<0.05) was significantly higher among transgender populations with self-injury or suicide behaviors than among those without. Multivariable logistic regression analysis showed that immature defense mechanisms increased the risk of depression (OR=1.034, 95%CI: 1.018-1.051) and anxiety (OR=1.031, 95%CI: 1.014-1.049) among transgender populations, while mature defense mechanisms reduced the risk of depression (OR=0.887, 95%CI: 0.832-0.946) and anxiety (OR=0.878, 95%CI: 0.821-0.938) among transgender populations. Conclusions The prevalence of depression and anxiety was 46.83% and 26.19% among transgender populations included in this study. Mature defense mechanisms are beneficial to reduce the risk of depression and anxiety among transgender populations. Key words： transgender population depression anxiety defense mechanism

Objective To explore the effect of silver needle thermo-conduction on cervical spondylosis of vertebral artery type (CSA). Methods 82 patients with CSA were randomly divided into control group (n=41) and treatment group (n=41). The control group was treated with cervical traction combined with microwave diathermy, and the treatment group was treated with silver needle thermo-conduction combined with cervical traction. Both groups were evaluated with Evaluation Scale for Cervical Vertigo (ESCV) and transcranial Doppler before and 1 course after treatment. Results The ESCV score, the peak blood flow velocity of period of contraction of left vertebral artery, right vertebral artery and basilar artery in both groups were significantly higher after treatment than before (P<0.01), the treatment group was better than the control group after treatment (P<0.01). Conclusion Silver needle thermo-conduction combined with cervical traction therapy can accelerate the blood flow of patients with CSA and improve their clinical symptoms

Oceanic carbon monoxide(CO) has been of biogeochemical interest due to its significant role in global carbon cycle and the greenhouse effect. A headspace method coupled with ta3000 trace gas analyzer system for the determination of CO in seawater was developed. The effects of temperature, equilibrium time and water/gas volume ratio on the sensitivity of headspace analysis were studied in detail. The results showed that CO concentrations in seawater were measured successfully by the 50-mL glass-only syringes with a water/gas volume ratio of 44∶ 6 and an equilibrium time of 5 min at 20 ℃ room temperature. Under the optimized conditions, the linear range of concentrations of CO was 0-2.7×10~(-6), r=0.999,p<0.0001. The relative standard deviation of the analysis method was <4.4%, with a detection limit of 0.02 nmol/L. The average recovery of CO was 90.5%. The concentrations of CO in surface waters of the North Yellow Sea were measured using this method and ranged from 0.20 nmol/L to 3.13 nmol/L, indicating that this method can be successfully applied to the detection of the in situ CO concentrations in seawater.

It was found that Ag nanoparticles (NPs) could enhance the chemiluminescence (CL) intensity of luminol- potassium ferricyanide system. On the basis of enhancement effect,a flow injection method was developed for the determination of terbutaline sulfate. The structure and shape of Ag NPs were characterized by transmission electron microscopy. The UV-Vis absorption spectra and chemiluminescence spectra suggested that new luminophor was not formed after Ag NPs introducing in luminol-potassium ferricyanide chemiluminescence system. A possible mechanism of Ag NPs strengthening on luminol-potassium ferricyanide CL reaction was also discussed. The effect of concentration of luminol,potassium ferricyanide,sodium hydroxide and Ag NPs on CL reaction was investigated respectively. In the optimum conditions,the linear range was 1.0×10~(-9) -2.0 ×10~(-5) g/L(r =0.9935) and the detection limit was 1×10~(-10) g/L. The relative standard deviation (RSD) was 3.6% for 1. 0 ×10~(-6) g/L terbutaline sulfate (n = 11 ). The recommended method has been successfully applied to the determination of terbutaline sulfate tablets and the recovery was between 98. 5% - 102. 5% ,moreover the results were almost identical with the same results of Pharmacopoeia method.