In exploring the reform of pathology teaching, we tried to utilize the PBL teaching mode with multimedia technology and got a good result. We found that the new teaching method not only can improve the quality of teaching, stimulate students' interest in pathology, but also can enhance the ability to analyze and solve the problem, and strengthen the learning initiative and enthusiasm at the same time. It is an advanced teaching method to cultivate good medical students by means of PBL teaching mode and multimedia technology. However, we still need further exploration on how to make good use of the new teaching methode.

Objective: To develop a computer-aided three-dimensional design software and laser scanning technique for the restoration of unilateral orbital defect. Method: By laser scanning apparatus plaster face model and the acquired primitive face point data were scanned; with the three-dimensional design software the point data were reconstructed to one smooth face surface image and the data of the defect of the orbital tissue were obtained from the normal contralateral tissue data by software. Results: The face-model data obtained by laser scanning apparatus was distinct and precise; the Digisurf retrograde engineer software succeeded to fulfill the computer-aided design for restoration of unilateral orbital defect. Conclusion: The laser scanning technique and three-dimensional design software can be used in the computer-aided design of the unilateral orbital defect restoration.

[Objective]To investigate the metaphase result of extensive fusion in treatment of spondylolisthesis combined with intervertebral disc degeneration of adjacent joints.[Methods]Seventy-nine cases of lumbar spondylolisthesis combined with intervertebral disc degeneration of adjacent joints were treated with extensive fusion and internal fixation from September 2002 to September 2007.JOA and VAS scores were assessed and compared pre-and post-operatively.X-ray and CT were performed to observe the stabilization of fusion joints,and satisfaction rate from patients was investigated.[Results]All patients were followed up for 2 to 6.5 years(mean,4.6 years).Post-operative JOA scores increased(P

The antagonistic effects of L-THP, Ver and Pap to contraction of isolated guinea pig trachea preparations induced by BaCl2 10-3mol/L ,KC1 3?10-2mol/L, 5-HT 2?10-5mol/L, Ach 2?10-5mol/L and His 2? 10-5mol/L were studied.The antagonistic effects of L-THP were different from Ver and Pap. The IC50 value of L-THP for contraction induced by BaCl2, KC1, 5-HT and His was 3 .5? 10-5mol/L , 4.5 ? 10-5mol/L , 2.5?10-3 mol/L and 8.9? 10-5mol/L respectively. It was showed that sensibility of several agonists to inhibition by L-THP was correlated with dependence of extracellular Ca2+. The potencies of L-THP on anti-KCl and anti-5-HT actions were similar. The results suggested that LTHP possesses anti-calcium and anti-5-HT action on guinea pig trachea.

Bone marrow infiltration in lymphoma is crucial to the disease diagnosis,staging,treatment options and prognostic evaluation.Although bone marrow biopsy is the main detecting method,it has high false negative rate.At present,18F-fluorodeoxyglucose positron emission tomography-computed tomography (18 F-FDG PET-CT) technology which is integrating morphological characteristics with metabolic function provides a new way for the diagnosis,staging and prognostic evaluation of bone marrow infiltration in lymphoma due to its advantages of high sensitivity,high specificity and functional image.

Objective To understand the status quo of the health statistics human resources in Chongqing municipality. Methods The status quo of the health statistic human resources in Chongqing municipality was investigated by the national health statistics reporting system.The age,seniority,qualifications,specialty,job title,distribution,etc.in the health personnel were ana-lyzed by the SPSS17.0 statistical software.Results 74.89% of health statistic personnel were part-time workers.In the vast ma-jority of medical institutions,the number of health statistician was 1 or 2.As to the education background,73.50% of the total stat-istician number only got the junior college degree even below.Only 216 statistic personnesls′major fitted to the staff positions.Ba-nan district in the major city,Tongnan county and Dazu district in the west area,Wushan county in the east area,Dianjiang county and other counties in central area,and the new urban development area were relatively lack of human resources of the health statistic personnel.Conclusion The health statistic team in Chongqing municipality has low stability,inadequate staff and poor professional quality.The distribution of statistic personnel human resources is uneven among the five functional areas and various districts and counties.

Objective To observe the expression of ZNF139 and MMP-7 in endometrial adenocarcinoma tissues and discuss the correlation with clinical pathological significances.Methods In 71 patients diagnosed as endometrial adenocarcinoma,71 cancer tissues(adenocarcinoma group) and 71 adjacent normal tiuues(adjacent cancer group) were collected in operations.Immunohistochemical methods was used to detect the expression of ZNF139 and MMP-7 in the two groups,the relationship between the two genes and communication with clinicopathological indexes were discussed.Western blot and qRT-PCR were used to detected the expression of ZNF139 and MMP-7 at protein and mRNA level.Results The positive rates of ZNF139 was 66.20% in adenocarcinoma group,higher than in adjacent cancer group(21.42%,P=0.016);the positive rates of MMP-7 was 74.65% in adenocarcinoma group,higher than7 than in adjacent cancer group(26.76%,P=0.012).The expression of ZNF139 and MMP-7 was correlated with lymph node metastasis,muscle invasion and FIGO stage(P=0.028,0.031;P=0.004,0.016;P=0.008,0.011).Spearman rank correlation analysis showed there was positive correlation between ZNF139 and MMP-7 in adenocarcinoma tissues(r=0.716,P=0.039).Western blot showed that ZNF139 and MMP-7 expression in adenocarcinoma group were higher than in adjacent cancer group(t=6.92,7.34;P<0.01);qRT-PCR showed that ZNF139 mRNA and MMP-7 mRNA expression in adenocarcinoma group were higher than in adjacent cancer group(t=4.27,4.06;P<0.05).Conclusion ZNF139 and MMP-7 are correlated with malignant pathological characteristics in endometrial adenocarcinoma,they may modulate the malignant behavior together.

In order to investigate the effect of vitamin A (VA) on the secretion of IFN-gamma and IL-4 in Mycoplasma Pneumoniae (MP)-induced A549 cells, A549 cells were co-cultured with MP for different time lengths and then the levels of IFN-gamma and IL-4 in the cell culture supernatants were detected before and after treatment with different concentrations of VA by using the enzyme-linked immunosorbent assay (ELISA). The results showed that the level of IFN-gamma and IL-4 in the supernatants of MP-induced A549 cells was much higher than that in non-induced cells (P<0.01). After application of VA, IL-4 level was not increased until the concentration of VA was up to 0.5x10(-5) mol/L (P<0.01). However, with concentration of VA increased up to 1x10(-4) mol/L, IL-4 was significantly suppressed (P<0.01). It was concluded that MP could induce the secretion of IFN-gamma and IL-4 in A549 cells. VA could inhibit the secretion of IFN-gamma and increase the IL-4 level in MP-induced A549 cells. However, high concentration of VA had an inhibitory effect on the secretion of IL-4 as well as on the IFN-gamma. These data provided a theoretical basis for the application of VA in MP pneumonia in the clinical practice.
Cell Line, Tumor ; Coculture Techniques ; Culture Techniques ; Interferon-gamma/*secretion ; Interleukin-4/*secretion ; Lung Neoplasms/pathology ; Mycoplasma pneumoniae/growth & development ; Mycoplasma pneumoniae/*physiology ; Vitamin A/*pharmacology

Objective To explore the application of bundle strategies in the management of multidrug-resistant or-ganisms(MDROs).Methods From July 1 ,2013,bundle intervention measures were applied in the management of MDROs from inpatients in a tertiary first-class hospital,the implementation of isolation measures for MDRO-infec-ted/colonized patients were monitored,April-June 2013 was pre-intervention group,July-September 2013 was the first stage of intervention,October-December 2013 was the second stage of intervention,the qualified rate of each measure and incidence of MDRO infection per 1 000 patient-days before and after intervention were compared. Results From April to December 2013,a total of 3 430 non-repetitive isolates were detected.The qualified rate of doctors’ order “contact isolation ”before intervention and in the second stage of intervention were 78.14% and 95.95% respective-ly;the qualified rate of hanging bed-side isolation signs,providing bed-side quick-drying hand disinfectant,and patients wearing blue wristbands before intervention were 52.70%,66.89%,and 87.16% respectively,and in the second interven-tion stage were all 100.00%.The qualified rate of disinfection of medical supplies and environment,as well as patients’ bed assignments before intervention were 23.65% and 15.54% respectively,in the second stage were 79.79% and 77.66% respectively.Health care workers’awareness rate of hand hygiene knowledge,hand hygiene compliance rate,and hand hygiene correct rate before intervention were 69.70%,45.76%,and 76.35% respectively;in the second stage were 90.23%,87.50%,and 94.58% respectively;the qualified rate of implementation of each isolation measure before inter-vention and in different stages after intervention were all significantly different(all P <0.01).The incidence of MDRO in-fection per 1 000 patient-days before intervention,in the first and second intervention stages were 3.24‰,2.63‰,and 2.20‰ respectively.Conclusion After the monitoring and intervention in MDROs with bundle management strategies,the qualified rate of each measure improved,incidence of MDRO infection per 1 000 patient-days decreased.