Objective To investigate the distribution and the infection status of human papillomavirus (HPV)genotype in male outpatients with sexually transmitted diseases (STD)in Northeast of Sichuan.Methods Totally 259 male outpatients with STD were performed to examine for HPV-DNA genotype by using Polymerase chain reaction and gene chip technolo-gy.Results 202 samples had positive HPV among 259 samples,the positive rate was 77.99%.Among 202 samples of HPV infection,60.89% were infected by single HPV genetype,26.24% were infected by two HPV genetypes,12.87% were in-fected by multiple HPV genetypes;27.41% were infected by high risk HPV genotypes,74.13% were infected by low risk HPV genotypes.In the rate of HPV genetype infection,the percentages of HPV6,HPV11,HPV16,HPV43,HPV33 and HPV58 were 52.97%,48.51%,16.34%,6.93%,5.45% and 5.45% respectively,and the rates of other HPV genetype in-fection were less than 5.00%.Among different age groups of men,there were insignificant differences in the infection rates of HPV (χ2=2.31,P>0.05),but there were statistical significance in the infection rates of high risk HPV subtypes (χ2=9.79,P<0.05).Conclusion The situation of HPV infection was comparatively severe and the mainly HPV infection types were low risk HPV genotypes infection or single HPV genetype infection in Northeast of Sichuan male outpatients with STD.The most frequent infection of high risk HPV subtypes was HPV16,and the nexts were HPV33 and HPV58;HPV6 and 1 1 subtypes were the most HPV subtypes among the low risk HPV infections.

Objective To investigate the specimen source and antibiotics resistance of Acinetobacter baumannii isolated from in‐patient in Intensive Care Unit(ICU) .Methods Specimen source and antibiotics resistance of 520 strains of Acinetobacter bauman‐nii ,isolated from patients of ICU in Affiliated Hospital of North Sichuan Medical College from 2011 to 2014 ,were retrospectively analyzed .Results The main source of Acinetobacter baumannii was sputum specimens ,accounting for 90 .4% .Acinetobacter bau‐mannii isolates showed the lowest resistance rates to cefoperazone‐sulbactam and minocycline(32 .0% and 25 .2% ,respectively) .A‐bout 68 .1% and 74 .9% of these strains were resistant to trimethoprim‐sulfamethoxazole and levofloxacin ,respectively .More than 86 .0% of the strains were resistant to other tested antibacterial agents .Conclusion Acinetobacter baumanii strains ,isolated from ICU ,could have high resistance rates to many kinds of antibacterial agents ,and cefoperazone‐sulbactam and minocycline might be with fine antibacterial activity against Acinetobacter baumanii .Drug resistance monitoring of Acinetobacter baumanii should be strengthened ,and antibacterial agents should be selected and used rationally according to the results of drug sensitivity test .

Clinical interns in the clinical microbiology laboratory not only need to accomplish the practice required in the syllabus but also need to be trained in the following aspects: laboratory biosafety, basic and important technical skills, potential guiding function of the original specimen smear, overall quality management, quick provide of microbiological sample information, communi-cation between microbiological laboratory and clinical departments, detection and significance of com-mon drug-resistant bacteria, etc. Based on these training, they might have a comprehensive and sys-tematic understanding of clinical microbiological testing and get improved in their professional quality and employment competitiveness thus to build a solid foundation for clinical work in the future.

Objective To investigate the specimen source and drug resistance in the strains of Acinetobacter baumannii isolated from the submitted samples in the neurosurgery wards of our hospital during 2011-2014 in order to provide the reference for clini‐cal treatment and nosocomial infection control .Methods A retrospective analysis was performed on the clinical data of clinical dis‐tribution and antibacterial drugs sensitivity in 404 non‐repeated strains of Acinetobacter baumannii isolated from the samples of neurosurgical patients .Results The mainly specimen source of Acinetobacter baumannii isolated from neurosurgical patients was sputum and cerebrospinal fluid ,accounting for 89 .1% and 7 .9% respectively .Acinetobacter baumannii isolates showed the lowest resistance rates to minocycline and cefoperazone/sulbactam (28 .6% and 31 .8% respectively) .The resistance rates to imipenem and meropenem were 79 .4% and 83 .2% respectively ;the resistance rate to other antibacterial drugs exceeded 69 .0% .Conclusion Acinetobacter baumanii strains isolated from the neurosurgery department have higher resistance rates to many kinds of antibacteri‐al agents ,minocycline and cefoperazone/sulbactam still has good in vitro antibacterial activity against Acinetobacter baumanii .Clinic should strengthen the management of antibacterial agents ,increases the rate of drug susceptibility test and rationally uses the anti‐bacterial drugs .

Objective To investigate the relationship between different genotype infection and multiple infection of human papil‐lomavirus(HPV) with cervical lesions among the women in Northeast region of Sichuan province .Methods The cervical exfoliated cells in 213 women with HPV infection were performed the HPV genetype detection by the gene chip technique and the cervical le‐sion degree was also detected .Results 213 cases of cervial lesion with positive HPV infection were divided into five groups accord‐ing to pathological examination results :chronic inflammation(110 cases) ,cervical intraepithelial neoplasia(CIN) Ⅰ (21 cases) ,CINⅡ (26 cases ) ,CIN Ⅲ (28 cases ) and cervical cancer (28 cases) .The high risk HPV infection was dominated by the genotype HPV16 ,58 ,33 ,18 and the low risk HPV infection was dominated by the HPV genotype 11 ,6 .The HPV genotype HPV11/6/16 , HPV16/33 ,HPV16/6 ,HPV16/58 and HPV16/18 infection were most common in the chronic cervial inflammation ,CIN Ⅰ ,CINⅡ ,CIN Ⅲ and cervical cancer groups .The constituent ratio of different cervical lesions had statistical difference between the simple high risk HPV infection group and the low risk HPV infection group (χ2 = 41 .01 ,P< 0 .01) .The simple genotyps and the mixed genotype HPV infection had no influence on the cervical lesion degree(χ2 = 5 .74 ,P> 0 .05) .Conclusion HPV 16 ,58 ,33 ,18 are the main high risk HPV genotypes among women in the northeast region of Sichuan province ,HPV 16 is significantly related with the cervial lesion degree ;multiple HPV genotype infection does not promote the progress of cervical lesion .

Objective To investigate the expression of microRNA-134 ( miR-134 ) , CREB and pCREB in the temporal lobe tissue of patients and epileptic rats and to explore their roles in pathogenesis of epilepsy.Methods Tempo-ral lobe tissue samples of 14 patients with refractory epilepsy and 10 non-epileptic patients, and hippocampus and brain tis-sue samples of 42 rats were used in this study.Forty-two healthy adult male Sprague-Dawley rats were randomly divided in-to 6 epilepsy groups (24 h, 72 h, 7 d, 14 d, 30 d, and 60 d after kindling epilepsy) and a normal control group (n=6 for all groups) .The rat model of epilepsy was generated by intraperitoneal injection of 127 mg/kg lithium chloride and 16-20 h later, 35 mg/kg pilocarpine.In the temporal lobe tissue of patients and hippocampal tissue of rats, the expression level of miR-134 was detected by real-time polymerase chain reaction.The expression levels of CREB and pCREB were de-termined by Western blot, and CREB and pCREB localization was assessed by immunohistochemistry.Results Compared with the control rats, the expression of miR-134 was significantly decreased in the temporal lobe tissue of experimental rats at 72 h,7 d,14 d, 60 d after kindling (P＜0.05),and no significant change at 24 h and 30 d after kindling (P>0.05). Expression of miR-134 in patients with refractory epilepsy was significantly lower than that of the controls ( P＜0.05 ) , while up-regulation of CREB expression was at the same time points (P＜0.05).Up-regulation of pCREB expression was at all the time points after kindling (P＜0.05).CREB and p-CREB expressions were seen in the nuclei of neurons, and significantly higher in patients with refractory epilepsy and epileptic rats.Conclusions The expression of miR-134 is sig-nificantly decreased and that of CREB and pCREB was significantly increased in the temporal lobe tissue of patients with re-fractory epilepsy and the hippocampal tissue of epileptic rats.These findings indicate that the signaling pathway of miR-134/CREB/pCREB may play an important role in the pathogenesis of epilepsy.

Objective To evaluate the effects of stepwise and fine-grained dietary management on the diet control of liver cirrhosis patients complicated with gastrointestinal hemorrhage. Methods Eighty liver cirrhosis patients complicated with gastrointestinal hemorrhage were assigned to experimental group (41 cases) and control group(39 cases) by random digits table method. The patients in experimental group were treated with stepwise and fine-grained dietary management and the patients in control group were given conventional dietary treatments. The compliance diet, mastery of nutrition knowledge, malnutrition risk at admission, intake food, discharge and 2 months after discharge between two groups were monitored. The incidence of rebleeding was tracked after 1 year of discharge. Results The incidence of malnutrition risk in Child-Pugh class C was higher than that in Child-Pugh class B( Z=-4.3, P<0.05 ) . The incidence of malnutrition risk in patients with high education level was lower than that in patients with lower education( r=-0.453, P<0.05 ). The experimental group significantly outperformed control group in mastery of nutrition knowledge at discharge:80(70, 84) points vs. 52(42, 64) points, in compliance diet and malnutrition risk after 2 months after discharge:86(76, 91)%vs. 53(46,57)%, 1(1, 2) points vs. 2(1,3) points, the rebleeding rate in experimental group was significantly less than that in control group within 1 year after discharge 14.6%(6/41) vs. 35.9%(14/39), and the differences were statistically significant between two groups ( χ2 = 4.226- 51.232, all P < 0.05 ). Conclusions Stepwise and fine-grained dietary management can improve the mastery of nutrition knowledge, compliance diet and nutritional status, and reduce the occurrence of rebleeding, worth popularizing further in clinical care of liver cirrhosis patients complicated with gastrointestinal hemorrhage.

OBJECTIVE:To establish the method for simultaneous determination of 5 saponins in Lonicerae Flos. METHODS:Using macranthoidin B as a reference,HPLC method was adopted to calculate the relative correction factor(RCF)of it to macran-thoidin A,dipsacoside B,macranthoside A and macranthoside B. The contents of above 4 saponins were calculated through RCF. Using the contents of saponins determined by external standard method as measured value,the calculated value was compared with measured value. RESULTS:The linear ranges of macranthoidin A,macranthoidin B,dipsacoside B,macranthoside A and macran-thoside B were 0.316-6.32 μg(r=0.9973),0.453-9.06 μg(r=0.9982),0.231-4.62 μg(r=0.9996),0.342-6.84 μg(r=0.9984) and 0.147-2.94 μg(r=0.9961),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.74%-104.51%(RSD=2.37%,n=6)、96.70%-103.20%(RSD=2.37%,n=6)、96.12%-103.61%(RSD=2.45%, n=6)、98.80%-104.70%(RSD=2.32%,n=6)、99.21%-102.92%(RSD=1.39%,n=6),respectively. There was no statistical sig-nificance between calculated value and measured value(P>0.05). CONCLUSIONS:The method is simple,precise,stable and re-producible. It can be used for the determination of saponins in Lonicerae Flos.

Objective To explore the clinical features and endoscopic manifestations of eosinophilic gastroente-ritis(EG)in children.Methods A retrospective analysis was conducted of the clinical manifestation,laboratory examination,endoscopy(upper and/or colonoscopy)performance,diagnosis and treatment of 49 cases of patients who were diagnosed as EG in Children's Hospital of Nanjing Medical University from July 2013 to July 2015.Results The common clinical manifestations of EG in children were hematochezia(23 cases),diarrhea(20 cases),vomiting(18 cases)and abdominal pain(15 cases).The ages of children admitted to hospital for the first time ranged from 1 month and 7 days old to 13 years and 7 months old,and the mean age was 59.4 months old,in which most patients were younger than 1 year old,accounting for 38.77％(19 cases)of all.Peripheral blood eosinophilia was present in 20 cases(40.82％)of the patients,and only 6/18 cases(33.33％)of the patients had elevated serum IgE.Upon endoscopic analysis,the lesions involved esophagus(4 cases),stomach(14 cases),duodenum(20 cases),small intestine(15 cases),colon(28 cases)and rectum(6 cases),and the most common manifestation under gastroscopy was mucosal hyperemia edema(27 cases)and erosion(9 cases),while the most common manifestation under colonoscopy was mucosal hyperemia edema(25 cases)and nodular hyperplasia(24 cases).All patients improved with food restriction,in which 8 cases were treated with glucocorticoid while 9 cases with oral Singulair and 9 cases with oral Loratadine.All children with symptoms were alleviated somewhat,but 5 cases of them relapsed after drug withdrawal.Conclusions The clinical manifestations of EG in children varied and were mainly hematochezia,vomiting,diarrhea and abdominal pain.Some patients had the elevated peripheral blood eosinophilia and serum IgE.The most common manifestations under gastroscopy were mucosal hyperemia edema and erosion while the most common manifestations under colonoscopy were mucosal hyperemia edema and nodular hyperplasia.

ObjectiveTo investigate the therapeutic effect of narrow band ultraviolet B ( NB VUB)and its influence on the expression of IL23/Th17 axis in peripheral blood of patients with Psoriasis vulgaris,and to further explore the mechanism of action of NB UVB.MethodsForty - eight cases of Psoriasis vulgaris were treated with NB-UVB irradiation for 20 times,the therapeutic effect was evaluated by the Psoriasis Area and Severity Index (PASI) scores.Peripheral blood was obtained from normal healthy controls and patients with Psoriasis vulgaris before and after NB-UVB irradiation.Three color flow cytometry was carried out to quantify Thl7 cells,and ELISA was used to examine the levels of serum IL17 and IL23.Results The mean PASI scores were significantly decreased after treatment with NB UVB irradiation[ (8.12±4.05)score vs (3.98±2.03) score,P＜0.01 ].Levels of Th17[ (2.78 ± 1.93)％ vs (0.98±0.56)％ ],IL-17[ (23.85±7.98) pg/ml vs (6.53±4.26) pg/ml] and IL-23 [ (29.73 ± 12.08)pg/ml vs ( 16.73±8.91 )pg/ml] were significantly higher in patients with Psoriasis vulgaris than that in healthy controls ( P ＜0.01 ).After treatment with NB-UVB irradiation,levels of Th17 [ ( 1.13 ± 0.51 ) ％ ],IL-17 [ ( 8.03±5.01 )pg/ml ],and IL-23 [ ( 17.03 ± 9.85 )pg/ml ] were significantly decreased than before ( P ＜ 0.01 ),and were positively correlated with PASI ( P ＜0.05).ConclusionsNB-UVB may affect IL23/IL17 to achieve its therapeutic effect on patients with Psoriasis vulgaris.