The Socialist legal construction needs to strengthen the legal education.It is significant to seek for the path of legal education in order to enhance the actual effect of legal education.The path of legal cducation includes traditional education,utilitarian education,reasonable education,legitimate education and feeling education,etc.We can get a better actual effect of legal education if different paths of legal education (traditional education,utilitarian education,reasonable education,legitimate education and feeling education) can be integrated,infiltrated and complemented.

The tumorigenesis of breast cancer is a multistep process with many factors.The microRNAs (miRNA) participates in the development and distance metastasis of tumor by regulating proliferation,apoptosis and migration of tumor cells.The study of the mechanisms that miRNA impacting breast cancer cell proliferation and metastasis may provide new ideas for the diagnosis and treatment of breast cancer.

Objective To explore the influences of indoor and outdoor sources of air pollutants on NO 2 concentra tions in indoor air.Methods The dynamic variations of NO 2 concentrations in indoor and outdoor air were continuously monitered by ML 9841B chemiluminescence continuous monitoring analyzer in2families of White haven town of west Cumbrra,UK.Results During the period of indoor air monitoring,typical background concentrations of NO 2 in air in the center of White haven were between19and28?g /m 3 (10min-mean),and rose to38-47?g /m 3 (10min-mean)during the morning and evening rush of traffic.NO 2 concentration increased about 190?g /m 3 (10min-mean)after the use of a gas cooker in the kitchen A1-hour mean concentration of253?g /m 3 was recorded in the kitchen air of one of the houses,which substantially exceeded the NAQS standard of200?g /m 3 .Conclusion The increase of NO 2 concentration in kitchen air induced by the use of gas appliances played a more important role in influencing the indoor air quality than outdoor air did.

Objective To investigate the gene polymorphism of HLA-DR locus and feature of HLA haplotypes of Han Population in Tianjin area.Methods Reverse PCR-SSOP typing results of HLA-DR alleles of 3000 cord blood units from Han population pregnant women in Tianjin area were compared to those of European Caucasian and the Han populations in Xi’an area.Results DRB1 *15, allele presented the highest frequency,followed by DRB1 *09,DRB1 *07,DRB1 *12,and DRB1 *04.The main haplotypes were A *02-DRB1 *09,A *02-DRB1 *15,B *13-DRB1 *07,A *02-DRB1 *12,A *30-DRB1 *07 and B *46-DRB1 *09 in sequence.The frequencies of DRB1 *12 and DRB1 *09 alleles were significantly higher than in European Caucasian,while the frequencies of DRB1*01 and DRB1 *03 alleles were significantly lower than those in European Caucasian.As compared with that of the Han population in Xi’an,there was not significantly difference except a decrease in frequencies of DRB1 *04 and DRB1 *13 alleles.Conclusions Distribution of the allelic polymorphism of HLA-DR locus was similar to those in northern Han population,such as in Xi’an area,and it was obviously different from those in European Caucasian.

Oxidative damage theory is currently one of the predominant theories on the mechanisms of aging. Previous research has shown that antioxidants can extend the lifespan in the model organism by scavenging free radicals,inducing the expression of stress related genes and hormesis. However, recent studies have suggested that these pharmaceuticals may cause serious side effects,such as promoting oxidation,increasing the risk of cancer,and destroying the metabolic balance. The low absorption and targeting property also limit the efficiency of most antioxidants. As a result ,the correlation between antioxidants and lifespan extension remains to be demonstrated. We reviewed the research progress in the field of lifespan extension by antioxidants in recent years and provided references for future research in related areas.

Background:Patients with refractory ulcerative colitis( UC ) cannot benefit from the conventional aminosalicylic acids,steroids and immunosuppressants. The emergence of biological agents offers new opportunities for these patients. Aims:To evaluate the clinical efficacy of infliximab for treating patients with refractory UC. Methods:Twelve patients with moderate to severe active UC admitted from Dec. 2012 to Jan. 2014 at the Second Affiliated Hospital of Harbin Medical University were retrospectively enrolled. All of them were steroids-refractory or steroids-dependent and infliximab therapy was then administered. Modified Mayo score was used to evaluate the disease activity and the efficacy of infliximab. Results:All patients completed the infliximab therapy and a 38-week follow-up. After treatment,the clinical manifestations were improved with varying degrees,and the efficacy at the 6th,22nd and 38th weeks was 66. 7%,83. 3% and 91. 7%, respectively,with significant differences between any two time points(P﹤0. 05). Conclusions:Infliximab is effective and practical for the treatment of refractory UC.

Objective To observe the clinical efficacy of electroacupuncture in treating peri-menopausal depression. Method Patients diagnosed with peri-menopausal depression were randomized into a Guanyuan (CV4) group (n=30) and a Neiguan (PC6) group (n=30). After 1-month treatment, Hamilton Depression Scale (HAMD), modified Kupperman index, plasm ACTH and serum CORT levels were observed and compared. Result After intervention, the observed indexes were decreased to different extents, and the between-group differences were statistically significant (P<0.05). Conclusion Electroacupuncture can effectively regulate the endocrine function of peri-menopausal depression patients, improve symptoms, and thus enhance the compensatory function and the quality of life, and the Guanyuan group shows a more significant effect.

Objective To investigate the expression of microRNA-134 ( miR-134 ) , CREB and pCREB in the temporal lobe tissue of patients and epileptic rats and to explore their roles in pathogenesis of epilepsy.Methods Tempo-ral lobe tissue samples of 14 patients with refractory epilepsy and 10 non-epileptic patients, and hippocampus and brain tis-sue samples of 42 rats were used in this study.Forty-two healthy adult male Sprague-Dawley rats were randomly divided in-to 6 epilepsy groups (24 h, 72 h, 7 d, 14 d, 30 d, and 60 d after kindling epilepsy) and a normal control group (n=6 for all groups) .The rat model of epilepsy was generated by intraperitoneal injection of 127 mg/kg lithium chloride and 16-20 h later, 35 mg/kg pilocarpine.In the temporal lobe tissue of patients and hippocampal tissue of rats, the expression level of miR-134 was detected by real-time polymerase chain reaction.The expression levels of CREB and pCREB were de-termined by Western blot, and CREB and pCREB localization was assessed by immunohistochemistry.Results Compared with the control rats, the expression of miR-134 was significantly decreased in the temporal lobe tissue of experimental rats at 72 h,7 d,14 d, 60 d after kindling (P＜0.05),and no significant change at 24 h and 30 d after kindling (P>0.05). Expression of miR-134 in patients with refractory epilepsy was significantly lower than that of the controls ( P＜0.05 ) , while up-regulation of CREB expression was at the same time points (P＜0.05).Up-regulation of pCREB expression was at all the time points after kindling (P＜0.05).CREB and p-CREB expressions were seen in the nuclei of neurons, and significantly higher in patients with refractory epilepsy and epileptic rats.Conclusions The expression of miR-134 is sig-nificantly decreased and that of CREB and pCREB was significantly increased in the temporal lobe tissue of patients with re-fractory epilepsy and the hippocampal tissue of epileptic rats.These findings indicate that the signaling pathway of miR-134/CREB/pCREB may play an important role in the pathogenesis of epilepsy.

BACKGROUND:It remains controversial in term of therapeutic efficacy of self-fixating mesh and sutured mesh in inguinal hernia repair. OBJECTIVE:To compare the therapeutic effects of self-fixating mesh and sutured mesh in open inguinal hernia repair with Meta-analysis. METHODS:Comprehensive electronic search strategies were developed using the folowing electronic databases: Cochrane library, PubMed, EMBASE, Medline, Ovid, CNKI, Wanfang and FMJS. The Literature published before December 2014 was searched. Perspective randomized controled trials about comparing self-fixating mesh and sutured mesh in open inguinal hernia repair were included. A data-extraction sheet was developed based on the preset standards. The data from eligible studies were pooled through Meta-analysis. RESULTS AND CONCLUSION:Nine trials with a total of 2 100 inguinal hernia patients met the inclusion criteria, including 1 033 cases of self-fixing mesh and 1 067 cases of sutured mesh. The Meta-analysis showed that no significant differences were found between the two groups in the recurrence rate, seroma, hematoma, wound infection, pain, foreign body sensations (P > 0.05), but the duration of operation was less in the self-fixing mesh group than the sutured mesh group (P < 0.05). According to limited evidence, there are some findings as folows: self-fixating mesh is equivalent to sutured mesh in the therapeutic effects on open inguinal hernia repair. Because of the limits of samples and literature quality, more large-sample and high-quality trials are required to make a definite clinical evidence to use self-fixating mesh for groin hernia repair.