An introduction of principle of chromatographic fingerprint spectrum and its application on guality standard of Chinese drug. The chromatogram is digitiged by its relative retention value. The digitized chromatogrzaphic fingerprint specturm of some chinese drug was obtained. This technic can be used to reseasch guality standard of chinese drug sucessfully.

Objective: To study the content of alkaloids in Huanglianjiedu Decoction and its disassemble prescription. Methods: The contents of four alkaloids such as berberine, jatrorrhizine, coptisine, palmatine in Huanglian jiedu Decoction and its disassemble prescription were determined by HPLC. Results: The content of four alkaloids extracted by tranditional decoction had a tendency to low. On the coutrary, when Rhizoma Coptis and Cortex Phellodendri were decocted together the quantity of alkaloids was additive, and when two herbs above decocted with Radix Scutella and Fructus Gardeniae the quantity of alkaloids decreased obviously. Conclusion:This study can provide a basis for establishing rational quanlitative standard for Huanglianjiedu Decoction.

Objective To explore the safety and therapeutic effect of Xuebijing injection for treatment of patients with capillary leak syndrome (CLS).Methods Seventy-seven patients with clinical diagnosis of CLS admitted to Intensive Care Unit (ICU) of the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine (TCM) from November 2015 to October 2016 were enrolled, they were divided into a control group (35 cases) and a Xuebijing group (42 cases) according to random number table method. The conventional treatment was given and at the same time the primary disease was actively treated in the control group; while in the Xuebijing group, on the basic treatment of the control group, additionally, Xuebijing injection 100 mL+ 0.9% normal saline (100 mL) was intravenously dripped, twice a day, 5 days constituting one therapeutic course. Before and after treatment for 5 days, the white blood cell count (WBC), neutrophils percentage (N), alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), serum creatinine (SCr), procalcitonin (PCT), pH value, partial pressure of blood oxygen (PaO2), blood lactic acid value (Lac), activated partial thromboplastin time (APTT), prothrombin time (PT), blood platelet count (PLT) in the patients of the two groups were compared; and the acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score was recorded; the length of stay in ICU, mechanical ventilation time, and 28-day survival rate were statistically calculated in two groups.Results After treatment, the levels of WBC, N, PCT, ALT, AST, BUN, SCr, Lac, APACHE Ⅱ score in Xuebijing group were lower than those in the control group [WBC(×109/L): 9.85±0.61 vs. 13.87±2.58, N: 0.75±0.08 vs. 0.90±0.10, PCT (μg/L): 1.13±0.71 vs. 4.99±1.38, ALT (U/L): 79.56±30.85 vs. 84.21±27.32, AST (U/L): 91.98±38.10 vs. 110.28±35.79, BUN (mmol/L): 7.35±0.82 vs. 8.57±1.43, SCr (μmol/L): 111.67±43.96 vs. 132.51±55.10, Lac (mmol/L): 1.88±1.01 vs. 3.31±1.46, APACHE Ⅱ score: 11.34±3.59 vs. 17.65±4.77]; the PaO2, PLT, 28-day survival rate in Xuebijing group were higher than those in the control group [PaO2 (mmHg, 1 mmHg = 0.133 kPa): 75.47±21.10 vs. 54.22±15.23, PLT (×109/L): 211.54±58.25 vs. 153.27±49.69, 28-day survival rate: 85.71% (36/42) vs. 71.43% (25/35), allP < 0.05]; the PT, APTT, ICU hospitalization time and mechanical ventilation time in Xuebijing group were shorter than those in the control group [PT (s): 13.62±2.11 vs. 18.45±4.26, APTT (s): 31.33±4.27 vs. 36.85±5.56, length of stay in ICU (days): 12.4±3.7 vs. 20.5±4.1, mechanical ventilation time (days): 10.5±4.9 vs. 18.7±5.5, allP < 0.05].Conclusion The application of Xuebijing injection for treatment of patients with CLS can relieve their disease situation, reduce inflammatory indicators, improve the blood coagulation function and hypoxemia, shorten the ICU hospitalization time and mechanical ventilation time, elevate the 28-day survival rate, and has no harmful effects on liver and kidney functions.

Standardization management of the procurement,supply,maintenance,filing,rejection and measurement of medical equipment is very important for determination of the right obligations and responsibilities of medical equipment management facility. It also avails to establish a set of systemic regulations to improve the performance of medical equipment facility and the continuous education of medical engineering technicians.

Influences of environment on medical equipment lie in many fields and exist independent of human consciousness.In order to lengthen the service life of medical equipment and produce the best possible results,human beings use scientific methods to utilize and change the natural situation.Bad influence from natural conditions to medical equipment is expatiated from such aspects as influence from thunder,rain,snow,electricity,warmth,humidity,mist,magnetism,etc.The management and the protection of the medical equipment under the natural situations are discussed.

Primary angiitis of the central nervous system is relatively rare. In recent years, research of the disease in neuroimaging, differential diagnosis, treatment and other aspects advanced greatly. This paper provided a systematic review of this disease entity in order to promote neurologists, neurosurgeons and radiologists′ experience in the diagnosis and treatment of the disease.

BACKGROUND: Collagen sponge is considered the most useful biomaterial owing to its excellent function and properties, easy processing, sterilization, and preservation and has been widely used in scientific research and clinical application. OBJECTIVE: To describe the properties of collagen sponge and to review the research progress of collagen sponge in clinical application in recent years. METHODS: A computer-based retrieval was performed by the first author to search for manuscripts published from January 2000 to August 2010 in Pubmeds and Elsevier databases and for manuscripts published from January 1993 to August 2010 in CNKI database using key words "collagen, collagen sponge, clinical application" in title and abstract items in English and Chinese language, respectively. RESULTS AND CONCLUSION: Collagen sponge, as a new biological material, has been widely applied in tissue engineering research in terms of hemostasis, wound healing, anti-infection, cartilage repair, and nerve repair. But at present, nearly all collagen is from animals and immunogenicity cannot be thoroughly eliminated. Researchers outside of China have synthesized recombinant human collagen using bioreactor and transgenic technology, but its efficacy and safety in clinical application needs further investigation and research.

AIM: To discuss the application of overlapping ratio to the fingerprints of plant and plant extract. METHODS: We took the HPLC fingerprints of green tea and tea polyphenols for decision procedure. RESULTS: Overlapping ratio could be applied to selecting representative samples for characteristic fingerprints of green tea and tea polyphenols,and could be used to deduce the raw material,process and producer of tea polyphenols.(CONCLUSION:) Overlapping ratio can be used to choose the suitable samples for establishing the characteristic fingerprint spectrum,and can supply references to relationship investigation among products,raw material and process.

Objective: To develop a HPLC method for simultaneous determination of catechins (EGC、DC、EGCG、EC、GCG、ECG) and caffeine in tea polyphenols and to quantify seven ingredients in twenty nine batches of tea polyphenols samples from thirteen producers. Methods: The study was achieved using a C 18 column with a methanol water 0.1% formic acid gradient elution system. Results: The peak resolutions of seven ingredients in mixed standards were all above 2; The resolutions in samples were all above 1.5 except EGCG; The average recoveries were 98.56%～100.14%. Conclusion: This method is accurate, stable, reproducible and suitable for quality control of tea polyphenols; the variations of contents are obvious among tea polyphenols samples from different even the same producers.

Fibroblast growth factor(FGF)-21 is a novel insulin-independent glucose regulator,and can be a potential therapeutics for treatment of type Ⅱ diabetes.Although FGF-21 was discovered recently,the insight into its biology and therapeutic utility is rapidly evolving.A number of key metabolically-linked molecules and pathways have been suggested to be involved in the mechanism of action of FGF-21,which enables us to renew the understanding of the FGF-21.The aim of this review is to report the update research outcomes.