1.Clinical Analysis of Death Causes in 100 Burn Patients
Xusheng LIU ; Hong YAN ; Xiaokun YANG
Journal of Chinese Physician 2000;0(11):-
Objective To analyze the death causes of the burn patients so as to explore the effective procedures which can raise burn management level. Methods One hundred patients died of burn injury during the past 20 years were enrolled in the study. The died patients were grouped as A (1984~1993) and B (1994~2003) groups, each group containing 50 cases. The mortality, burn area and depth, etiology, pre-hospital treatment, admission time, survival time, tracheostomy, the application of respirator and fibrobronchoscope, operation times, continuous renal replace treatment (CRRT), the incidence of inhalation injury and the pathogenesis of burn death were analyzed and compared between A and B groups. Results There were no differences in burn severity (area and degree), etiology and causes of burn death between the two groups. But the mortality in B group (1%) was evidently lower than that in A group (2%, P
2.The Construction of Eukaryotic Expression Vector Containing CYP2B1 Suicide Gene
Zhizhong LIU ; Xiaokun CAI ; Jusheng LIN
Journal of Chinese Physician 2001;0(01):-
Objective To construct an expression vector harboring CYP2B1 suicide gene, and detect its expressions in tumor cell lines. Methods PCR amplification was performed using primers based on murine CYP2B1 gene sequence from gene bank and pc3/2B1 as template. PCR product was directly inserted an eukaryotic expression plasmid pcDNA3.0. The recombinants were analyzed and identified by restriction enzyme analysis, PCR and sequencing. Then the recombinant vector pcDNA3.0/CYP2B1 was transfected into three tumor cell lines by liposome-mediated method. The expressions of CYP2B1 gene in all the cell lines were detected by RT-PCR method. Results pCDNA3.0/CYP2B1 vector was successfully constructed, and could express CYP2B1 mRNA in the three tumor cell lines. Conclusion Eukaryotic expression vector pcDNA3.0/CYP2B1 containing CYP2B1 gene under the control of a CMV promoter is an novel effective expression vector for tumor gene therapy.
3.Experimental research on the role of acidic fibroblast growth factor in the survival and proliferation of renal tubular epithelial cells in vitro
Limin LIU ; Huagang LIU ; Juen HUANG ; Xiaokun LI ; Jian XIAO
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To explore effects of acidic fibroblast growth factor(aFGF)on the growth of renal tubular epithelial cells cultured in vitro. Methods Renal tubular epithelial cells in rats were gained through digestion by pamcreatin. aFGF was added into cultures of renal tubular epithelial cells, as experimental groups. The number of cells was counted and the shape of cells was observed at 12,24,48 and 72 h. Results Renal tubular epithelial cells were successfully obtained from kidneys. After treated for 72 h,the renal tubular epithelial cells showed different proliferation in experimental and control groups. There was no obvious difference at 12 h, but there was statistical difference between the two groups at 24 h,48h and 72 h(P
4.Diagnostic significance of urethral sphincter electromyography and external anal sphincter electromyography in patients with multiple system atrophy
Feng QIU ; Xiaokun QI ; Liping LI ; Jianguo LIU ; Wei YAO
Chinese Journal of Neurology 2012;45(3):188-191
Objective To assess the significance of urethral sphincter electromyography (US-EMG) and external anal sphincter electromyography (EAS-EMG) for the diagnosis of multiple system atrophy (MSA).Methods US-EMG and EAS-EMG were performed in 9 patients who were diagnosed as MSA.Duration,motor unit action potentials amplitude,polyphasicity,as well as recruited pattern and amplitude during powerful contraction were recorded and analyzed.Results Among 9 patients who were diagnosed as MSA,7 cases showed neural injury by both US-EMG and EAS-EMG.There was significant difference of electromyographic findings between US-EMG group and EAS-EMG group (average volatility (μV):1063.44 ±499.92 vs 634.89 ±265.07; polyphasic wave:0(0,20% ) vs 57% (28%,63% ) ; t =2.567,P=0.033;t =2.833,P=0.012).Conclusions Although US-EMG may be difficult to perform,US-EMG may have the same specificity as EAS-EMG for the diagnosis of MSA,especially for the diagnosis of MSA patients only with urination disorders,who are involved in Onuf neclear according to some of the abnormal indexes.
5.The diagnostic value of urethral sphincter electromyography in patients with multiple system atrophy
Xiaokun QI ; Liping LI ; Wei YAO ; Jianguo LIU ; Feng QIU
Chinese Journal of Internal Medicine 2012;(12):975-977
Objective To analyze diagnostic evaluation of urethral sphincter electromyography (US-EMGs) for patients with multiple system atrophy (MSA).Methods Totally 15 patients who were diagnosed as MSA were examined as treatment group while 17 non-MSA patients were examined as controls.US-EMGs were performed in the both groups.Spontaneous activities when relax,parameters of motor unit potentials(MUPs) mean duration and amplitude,percentage of polyphasic ware,satellite potential,recruitment potentials and amplitude when strong contraction were recorded and analyzed.Results USEMGs changes of various abnormalities were found in 13 cases (86.7%) in MSA group.There were significant differences of electromyographic findings between the MSA group and control group including MUPs mean duration[(12.79 ±3.18)ms vs (9.49 ± 1.51)ms] and amplitude[(828.53 ±459.89) μV vs (378.76 ± 152.26) μV] as well as recruitment potentials [(11.47 ± 21.55) % vs (8.23 ± 10.74) %] and amplitude [(2.19 ± 1.24) mV vs (0.75 ± 0.42) mV] when strong contraction (all P values < 0.05).Conclusions There is certain value of US-EMGs for the diagnosis of MSA.It could be used as a routine electrophysiological method for the patients who are suspected of MSA.It could be a supplement of externalanal sphincter electromyography.
6.Treatment of bilateral hydronephrosis caused by prostate cancer
Yinhuai WANG ; Gan YAO ; Xiaokun ZHAO ; Ren LIU ; Luoyan YANG
Chinese Journal of Urology 2008;29(7):486-488
Objective To evaluate the treatment of bilateral hydronephrosis caused by prostate cancer. Methods Twenty-four eases with mean age of 71 years old (ranging from 64--81 years old) were diagnosed with bilateral hydronephrosis caused by prostate cancer and treated with complete androgen deprivation. Surgical castration plus Bicalutamide 50 mg/d was offered to 18 eases and medical castration (Goserelin, 3. 6 mg/month) plus Bicalutamide 50 mg/d was offered to 6 cases. There were 19 cases developed severe lower urinary tract symptoms. Among these 19 cases, 13 cases had accepted Foley catheter and 6 cases accepted suprapubic tube drainage. Results Before and after the treatment, the prostate volume decreased from (70. 3±11.2)ml to (42.6±15.8)ml(P=0. 001). Total PSA decreased from (40. 3±27.2)ng/ml to (9.5±8.3)ng/ml(P=0.02). Of the 24 cases, hydrone phrosis improved in 18 cases, remained unchanged in 3 cases and deteriorated in 3 cases. There were 14 patients developed renal insufficiency. After the treatment, Serum urea nitrogen decreased from (12. 8±6. 5) mmol/L to (6. 3 ± 4. 2) mmol/L (P = 0. 004) and serum ereatinine decreased from (206.8±152.3)μmol/L to (85.3±43.6)μmol/L(P=0.03), respectively. For those 6 cases with hy dronephrosis unchanged or deteriorated during the treatment, 4 cases accepted percutaneous nephros tomy and 2 cases accepted chtaneous ureterostomy. Conclusion The combination of complete androgen deprivation and bladder drainage through Foley catheter or suprapubic tube is an effective option in the treatment of bilateral hydronephrosis caused by prostate cancer.
7.The clinical,neuroimaging and electrophysiological presentations in 62 cases with multiple system atrophy
Feng QIU ; Xiaokun QI ; Sheng YAO ; Liping LI ; Jianguo LIU
Chinese Journal of Neurology 2009;42(7):471-474
Objective To evaluate the clinical,neuroimaging and electrophysiology features of 62 patients with multiple system atrophy(MSA).Methods Sixty-two cases with diagnosis of probable MSA were recruited in a retrospective studied.Clinical,neuroimage and external anal sphincter electromyography (EAS-EMG)data was retrospectively analyzed.Results In 62 cases(44 male and 18 female),the onset age was between 37 and 76.Among them,29 cases(46.8 % )were MSA-A,with orthostatie hypotension as the main clinical manifestation;24 cases(38.7 % )were MSA-C,with cerebellar ataxia ag the main chnical manifestation;9 cases(14.5 % )were MSA-P,with extrapyramidal symptoms as the main clinical manifestation.MRI showed that main lesion of MSA-A was in the cerebellum:that of MSA-C was in the cerebellum,pons and medulla;and that of MSA-P was in the putamen.Fifty-one cases did EAS-EMG and 46 cases showed neurogenie impairments.Nineteen cases were initially misdiagnosed with other diseases.Conclusions MSA is easy to be omitted or misdiagnosed at early stage.The diagnostie rate of MSA can be increased by the combination of clinical expressions,neuroimage,EAS-EMG and other necessary examinations.
8.Effect and nursing of enhanced external counterpulsation on patients with ischemic cerebral apoplexy
Juan ZHANG ; Liyin ZENG ; Shujuan LIU ; Yuguang WANG ; Xiaokun YAN
Modern Clinical Nursing 2016;15(7):38-41
Objective To explore the curative effect and nursing of enhanced external counterpulsation (EECP) on patients with ischemic cerebral apoplexy. Methods In September 2013 to March 2013 in Shenzhen Futian District People′s Hospital neurology hospital, toally 286 patients with ischemic cerebral apoplexy were randomly divided into two groups, control group and treatment group with 143 cases in each group. The control group used conventional treatment and the treatment group used EECP. The score of clinical nerve function and defect improved Barthel index were compared. Result The score of clinical nerve function defect and improved Barthel index of the treatment group before the treatment were without difference (all P>0.05). The score of clinical nerve function defect and improved Barthel index of the treatment group were less than those of control group after the treatment (all P < 0.05). Conclusion Ischemic stroke patients with positive EECP can significantly increase clinical neural function and life ability , improve patient′s quality of life.
9.Surgical treatment of pancreatic neuroendocrine neoplasms
Zhengcai LIU ; Bo HU ; Xiaokun HAO ; Haimin LI
Chinese Journal of Endocrine Surgery 2016;10(3):177-179
Pancreatic neuroendocrine neoplasms (pNENs) are clinically rare,however,it shows an in creasing trend recent years.Surgical management,such as radical resection or debulking,plays an important role in this field.Different procedures can be applied to tumors with different types,locations,and developing stages.The optimal clinical management of pNENs involves a multidisciplinary approach in order to improve prognosis.
10.Effect of basic fibroblast growth factor on radiation-induced splenocytic apoptosis of mice
An HONG ; Yanli XU ; Xiaokun LI ; Jian LIN ; Shuzheng LIU
Chinese Journal of Pathophysiology 1986;0(02):-
AIM: To investigate the effect of basic fibroblast growth factor (bFGF) on radiation-induced splenocytic apoptosis of mice. METHODS: At 14 h after whole body irradiation with 0.5 Gy and 1.0 Gy,splenocytes were cultured with and without bFGF,and splenocytic apoptosis was quantitatively analysed by flow cytometry. Cell proliferation was determined by the method of [3H]-TdR incorporation. RESULTS: bFGF(1 ?g/mL and 2 ?g/mL) could reduce the rate of cell apoptosis ,and promote the proliferation of splenocytes. CONCLUSION: bFGF could inhibit radiation-induced splenocytic apoptosis and promote the proliferation of splenocytes and then enhance body immunity.