Objective To analyze the data of HIV antibody mass screening in Tianjin from 2005 to 2006 and to evaluate current screening strategy.Methods Confirmation tests were conducted for 601 samples received form HIV screening laboratories in Tianjin in line with the procedures and methods defined by the National Guideline for HIV/AIDS Detection.Results Of the 601 samples,215 were HIV antibody positive(with complete bands);24 samples were positive with special reactive blots,of which 10 cases were followed up with 7 confirmed to be infected with HIV and with 3 confirmed to be excluded;51 samples were uncertain with indeterminate blots,of which 8 cases were followed up and all were proved to be HIV antibody negative.Conclusion Routine HIV screening as part of medical examinations for patients in health care settings or for detained personnel in police and judicial organizations represents an important approach to detect cases of HIV infection,Samples with special reactive blots or indeterminate blots should be handled with caution;they should be followed up and may be tested by other approaches,such as blot test or viral genome detection.

Objective To establish the normal reference ranges of peripheral blood CD4 and CD8 lymphocytes for the healthy population in Tianjin. Methods 366 samples were recruited from among local physical examination adults and their absolute and proportional values for the ranges of CD4 and CD8 lymphocytes were detected by quantitative flow cytometry with fluorescence labeled antibodies. Results Mean absolute values were (860.27?255.15) cells/?l for CD4 T cells,(671.60?371.39) cells/?l for CD8 T cells and (1.44?0.62) for CD4/CD8 ratio; and the proportional values were (37.05?7.42)% for CD4 T cells,(28.42?7.46)% for CD8 T cells. Our results showed no differences between gender and age on CD4 T cell counts among the healthy adult population in Tianjin. Conclusions There is difference in CD4 T lymphocyte counts in different areas.

The serum samples and corresponding cervical swabs were collected fi'om 50 women with genital warts from Tianjin city,China.The neutralizing antibodies against HPV-16,-18,-58,-45,-6 and-11 in serum samples were tested by using pseudovirus-based neutralization assays and HPV DNAs in cervical swabs were also tested by using a typing kit that can detect 21 types of HPV.The results revealed that 36%(18/50)of sera were positive for type-specific neutralizing antibodies with a titer range of 160-2560,of which 22%(11/50),12%(6/50),10%(5/50),4%(2/50),4%(2/50)and 2%(1/50)were against HPVs-6,-16,-18,-58,.45 and-11,respectively.Additionally,60%(30/50)of samples were HPV DNA-positive,in which the most common types detected were HPV-68(18%),HPV-16(14%),HPV-58(12%),HPV-33(8%)and HPV-6,HPV-11,HPV-18 and HPV-52(6% each).The concordance between HPV DNA and corresponding neutralizing antibodies was 56%(28/50)with a significant difference(P＜0.05).The full-length sequences of five HPV types(HPV-42,-52,-53,-58 and-68)were determined and exhibited 98%-100% identities with their reported genomes.The present data may have utility for investigating the natural history of HPV infection and promote the development of HPV vaccines.

Objective To obtain a simple method to treat segmental vitiligo. Methods The grafting was carried out as following: the epidermal cellular suspension was obtained from leg skin by razor blade after trypsinization. The suspension of epidermal cells were injected into depigmented lesion blisters which were produced by freezing with nitrogen. Results All of the patients which received treatment had successful repigmentation. Conclusion This technique is an effective and simple method for treating patients with segmental vitiligo.

Objective To compare main adverse cardiac events of patients with coronary heart disease and diametes,but normal lipids after statin on lowering lipids , and to bring out necessity of early lowering lipids . Methods A total of 324 patients with coronary heart disease and diametes were enrolled ,LDL-C was measured in-hospital for all patients and LDL-C<3.36mmol/L suitable for the experiment .They were divided into statin group (n=176,rosuvastatin 10 mg/d)and control group(n=148)randomly,all patients received second prevention for cor-onary heart disease including anti-platelet,anti-myocardial ischemia,smoke quitting,adjust diets and exercise,the CAG or PTCA(and stent implanting),CABG.the end events were main adverse cardiac events (cardiac events,recur-rent angina and target vessel reconstruction ).The mean follow-up time was(13 ±6)months.Results The fasting plasma glucose,LDL-C,hs-CRP in statin group were all significantly lower than those of control group [FPG:(5.3 ± 0.9)vs.(6.7 ±1.2)mmol/L,P=0.042;LDL-C:(1.7 ±0.6)mmol/Lvs.(3.9 ±1.6)mmol/L,P<0.01;hs-CRP:(1.4 ±0.5)mmol/L vs.(3.8 ±1.2)mg/L,P <0.01],MACE were less(15.6% vs.37.1%,P =0.032). Conclusion Early statin on patient with coronary heart disease and diametes ,though normal lipids ,which can down-regulate main adverse cardiac events significantly .

Objective To track the effects of hyperbaric oxygen (HBO) therapy on neonatal rats with hypoxic-ischernic brain damage (HIBD).MethodsA total of 126 healthy,seven-day-old SD rats were used to model the HIBD and then randomly divided into seven groups of 18:a sham group,a hypoxic-ischemic (HI) group,a 6 h HBO group,a 24 h HBO group,a 48 h HBO group,a 72 h HBO group and a 1 week HBO group.One hour of HBO treatment at 2 atmospheres absolute pressure was administered once daily for a week to the rats in the latter 5groups,starting at 6,24,48,72 hours and 1 week post the HIBD modeling,while no HBO was administered to the sham and HI groups.The effects were assessed in terms of histological changes ( the neuron density and the percentage of neuron apoptosis in the CA1 region of the hippocampus) and nitric oxide (NO),malondialdehyde (MDA) and superoxygen dismustase (SOD) concentrations after 15 days.ResultsIn the 6 h HBO,24 h HBO,48 h HBO and 72 h HBO groups,average neuron density in the CA1 region was significantly higher than in the HI group.But in the 1 week HBO group the average density was not significantly different than in the HI group.In the 6 h HBO,24 h HBO,48 h HBO and 72 h HBO groups the average percentage of neuron cell apoptosis in the CA1 area of the hippocampus was significantly lower than in the HI group,but the 1 week HBO group again showed no significant difference.There were significant differences in average NO,MDA or SOD levels among the 6 h HBO,24 h HBO,48 h HBO,72 h HBO and HI groups,but the 1 week HBO group showed no significantly higher average levels than the HI group.ConclusionsThe optimal therapeutic window for using HBO to protect against HIBD is within the first week.The best effect can be obtained in the first 6 hours,but after 1 week HBO no longer has a significant effect.The earlier the HBO is administered,the better the effect obtained.

Objective To explore the clinical features and the gene mutations in MECP 2 duplication syndrome. Methods The clinical data of a child with developmental retardation and hypophrenia accompanied with respiratory tract infection was analyzed retrospectively. Microarray analysis technique was used to detect the genes in the patient and his family. The pertinent literature was reviewed. Results A 1-year and 7-month old boy was found to have hypotonia, developmental delay, and recurrent respiratory tract infections after birth. Microarray analysis showed a duplication of 441.88kb in Xq28 area and diagnosis of MECP2 duplication syndrome was confirmed. His grandmother, mother, and two aunts were found duplication of 441.73-441.88kb in Xq28 area, all of whom were MECP2’s female carrier. Conclusions The improvement of chromosome chip technology inspection is helpful to the early diagnosis of MECP2 duplication syndrome.

Objective:To explore the understanding among medical rehabilitation staff about pain in children with cerebral palsy.Methods:A questionnaire was developed by referring to the literature on children′s rehabilitation and pain. It was used to survey 856 rehabilitation personnel including 110 doctors, 313 therapists and 433 nurses from 12 hospitals in 10 provinces using an online platform at www.wjx.cn.Results:The overall rate of correct responses was (67.89±13.35) %. Doctors and therapists scored about 70% correct and nurses about 65%. There were significant differences in pain knowledge related with educational background, age, work experience and position. Gender, professional title and parent status were not significant predictors. About 93% of those surveyed thought it was necessary to provide pain-related training, and 95% were willing to receive such training.Conclusions:There is a lack of knowledge about pain in children with cerebral palsy among rehabilitation medical staff. It is urgent to provide more related training and guidance to those in clinical practice.

Objective To observe the therapeutic effect of transcutaneous electric nerve stimulation on children with flaccid cerebral palsy. Methods 40 children with flaccid cerebral palsy were divided into 2 groups: treatment group (n=20) and control group (n=20). 2 groups were given conventional rehabilitation training for 30 d, while the treatment group added transcutaneous electric nerve stimulation on quadriceps femoris for 30 d. Quadriceps femoris was assessed by Modified Lovett classification, gross motor function was assessed by Gross Motor Function Measure (GMFM), and integrated electromyogram (iEMG) and root mean square (RMS) were recorded. Results There was significant improvement in 2 groups in Modified Lovett classification, GMFM, and iEMG and RMS (P<0.05) while the treatment group was better than the control group (P<0.05). Conclusion Transcutaneous electric nerve stimulation can enhance the muscle tone and strength of quadriceps femoris to improve gross motor function for children with flaccid cerebral palsy.

Objective:To explore any effect of orofacial myofunction therapy (OMT) on the swallowing and speech of children with Pierre-Robin malformation (PRS) after a corrective operation.Methods:Fifty-two children with dysphagia caused by PRS mandibular distraction osteogenesis were randomly divided into an observation group and a control group, each of 26. All were given routine nutrition support, but the observation group was additionally provided with OMT. Swallowing disorders, nutritional risk and body mass index were compared between the two groups after 6 months of the intervention. Orofacial muscle functioning and speech and language disorders were also compared between the two groups at the age of 3.Results:After the 6-month intervention the average Ichiro Fujishima dysphagia score in the observation group had improved significantly compared with before treatment and with the control group. The observation group′s nutritional status was also significantly better. At age three the orofacial myofunction classification of the observation group remained significantly better, on average, than that in the control group, and dysarthria or language retardation was significantly less prevalent.Conclusion:OMT can significantly improve the swallowing function, speech and nutritional status of children after surgery to correct PRS. This therapy is worthy of promotion and clinical application.