1.Detection and Susceptibility to Antibiotics of Mycoplasma in Genitourinary Tract
Chinese Journal of Nosocomiology 2009;0(17):-
OBJECTIVE To investigate the infection status and antibiotic susceptibility of Mycoplasma in our area,so as to instruct the rational use of antibiotics.METHODS Genitourinary secretions were collected with swabs.They were cultured with the diagnostic kit of Mycoplasma(Biomerieux Company).Meanwhile the susceptibility of Mycoplasma against 9 antimicrobial agents was tested with the same kit.According to the instrnction of the kit,the results were read.The data were statistically analyzed.RESULTS A total of 382 samples were collected,and the positive rate was 44.8%.which were composed by Uu 69.6%,Mh2.9%,and Uu+Mh infection 8.8% and low titer Uu+Mh infection 18.7%.The result of drug sensitivity showed that sensitivity of Mycoplasma to pristinamycin PRI,86.5% was the highest,then was to JOS(84.8%)and CIP(26.3%).CONCLUSIONS Ureaplasma urealyticum is the major cause of infection in genitourinary system.It shows serious resistance to commonly used antibiotics.Sensitive antibiotics should be selected based on the results of bacterial culture and drug sensitivity tests so as to raise the clinical curative effects.
2.Biomarkers of bladder cancer
Journal of Medical Postgraduates 2003;0(12):-
Bladder cancer is a common tumor with a high rate of recurrence and quick progression.Many biomarkers for the detection of bladder cancer have been identified,but none has been reported to date to have sufficient sensitivity and specificity.Recent researches are concentrated on p53,Rb,Cyclin D,P27kip-1,FGFR3,Cadherin,Survivin,Aurora,MMP,VEGF and DNA Methylation.In spite of their different sensitivities for bladder cancer of different stages or degrees,these biomarkers are valuable for its diagnosis and prognosis.
3.Separation technology and development of protomics
Journal of Medical Postgraduates 2003;0(10):-
Protein separation method is the core factor of the proteomics strategy. There are three methods for the protein separation, include two dimensional gel electrophoresis, liquid-phase electrophoresis and Mass Spectrometry, and protein-chip. In this article, the principles, processos, and development on the three separation protein methods.were reviewed.
4.Advance on insulin-like growth factor binding protein in human malignant tumors
Journal of Medical Postgraduates 2004;0(02):-
Insulin-like growth factors, their receptors and insulin-like growth factor binding protein constitutes insulin-like growth factor system. Studies in vivo and vitro suggest that they can regulate cell growth and proliferation. Insulin-like growth factors have a potent mitogenic effects which dependent on interaction between insulin-like growth factors and their receptors. IGFBP family contains six high-affinity members with variable functions and mechanisms of actions. In addition to functioning as simple carrier proteins, IGFBP can inhibit or promote IGFs by IGF-dependent or IGF-independent mechanism. Recent findings show that the IGFBP is correlated with the initiation and progression of tumor growth.
5.Diffuse large B-cell lymphoma
Journal of Medical Postgraduates 2003;0(03):-
Diffuse large B-cell lymphoma(DLBCL),the largest category of lymphoma,is a clinically and biologically heterogeneous disease entity.Immunophenotype,tissue microarray and molecular studies underline the extreme heterogeneity of DLBCL and suggest a subclassification of the tumor.This review summarizes the morphological,immunophenotypic,cytogenetic and molecular genetic features of DLBCL relevant to the precise prognostic previsions.
6.Eph receptor tyrosine kinase and its relation to cancer
Journal of Medical Postgraduates 2003;0(10):-
Eph,the largest subfamily of tyrosine kinase receptor, could be divided into two groups,EphA and EphB.Ephs play a role in the embryonic development and differentiation of the nervous and vascular systems. In recent years,a large number of ephrins and Ephs had been found to be overexpressed in numerous human tumors,and have relation to development and prognosis of cancers.Down-regulated of EphA3 and EphA7 in cancers,was attributed to the hypermethylaiton of CpG island in promoter region.
7.Down-regulation of EphA7 by aberrant methylation in colorectal cancer
Journal of Medical Postgraduates 2004;0(01):-
Objective:Ephs have been found to be over-expressed in numerous human tumors,and relate to the development and prognosis of cancers.The expression of EphA7 in colon cancer cell lines and primary colorectal cancers was detected.The role of EphA7 in the development of colorectal cancer was investigated. Methods:Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of EphA7.Methylation specific PCR and bisulfite sequencing were used to examine the methylation status of EphA7.pEGFP-EphA7 plasmid was constructed and was transfected into HCT116 which lost the expression of EphA7.Gene chip was analyzed for the expression of genes between transfectant and mock cell. Results:Down-regulation of EphA7 was observed in cell lines of primary colorectal cancers,and the evidence of aberrant methylation was found in CpG island of EphA7 promoter.The methylation status of EphA7 gene was related to sex,differentiation and location of colorectal cancers.Some genes were up or down regulated in pEGFP-EphA7 transfectant. Conclusion: EphA7 gene may be involved in the carcinogenesis of colorectal cancer.The hypermethylation of CpG island is an important mechanism leading to the down regulation of EphA7 in colorectal cancer.
8.Data transformation of No.1 Military Medical Project in section division
Chinese Medical Equipment Journal 1989;0(04):-
When a section is divided into two or more sections in hospital,patients must be assigned to the new section along with all medical data.With SQL commands,data is transferred according to the patient's section division.This method is also fit for the case that the section is divided while the nursing unit is not.
9.Advances on 14-3-3sigma protein in human malignant tumors
Journal of Medical Postgraduates 2003;0(04):-
14-3-3 protein is a highly conserved soluble acid family. Recent researches suggest that they play critical regulatory roles in cellular processes such as cellular mitosis,growth,differentiation,proliferation and death,etc.14-3-3sigma protein,one member of the family,expresses mainly in epithelial cells and has histological specificity.14-3-3sigma protein interacts with several signal transduction proteins,induces cell cycle arrest in G2 phase after DNA damage,and regulates cellular biological functions.Abnormal expression of 14-3-3? sigma protein is common during tumor transformation,which may be induced by p53 mutation or CpG island hypermethylation of promoter. It is closely related with the transformation,progress and prognosis of human malignant tumors.
10.Advance of study on lymphangiogenesis in the differentiated thyroid neoplasm
Journal of Medical Postgraduates 2003;0(12):-
Papillary carcinoma is one of the commonest tumors in the thyroid.The pathologic diagnosis of papillary carcinoma as well as follicular carcinoma and follicular adenoma of the thyroid are based on the morphology observation hitherto.However,the diagnostic value of immunostaining for distinction among differentiated neoplasm of the thyroid was so far absent.With the identification of the special markers of lymphatic vessel endothelial cell by immunohistochemical method in the recent years,the rapid progress in the research of lymphangiogenesis of the tumors had been made.This article briefly reviewed the progress of the special markers of lymphatic vessel endothelial cell and vascular endothelial growth factors in the differentiated neoplasm of the thyroid.