The dada of 987 out-patients aged 60 or over,who visited Bajiao Community Health Center of Beijing Shijingshan District from January 2006 to December 2009,were retrospectively analyzed.The results revealed that there was no gender difference in the prevalence of low vision and blindness ( χ2 =2.719,P = 0.606); the severity of low vision and blindness was correlated with the age of patients ( χ2 =138.389,P = 0.000); cataract was the main cause of low vision and blindness,accounting for 49.6%(62/125) of low vision and 64.3% (36/56) of blindness.

BACKGROUND:Biliary tract stents comprise two types:expanding metallic stents and plastic stents,the patency time of the former is shorter than the latter,but the cost is higher.Thus,it is dispute which kinds of stent should be selected.Accordingly,it becomes a research topic how to improve patency rate and prolong patency time using cheap stents.OBJECTIVE:To explain the progress of biliary tract stents and to explore the effects of materials and types of biliary tract stents on patency rates and patency times.METHODS:The Chinese Journal Full-text Database and Medline were retrieved by the first author using search terms of "biliary stent,metallic stent,plastic stent,occlusion and stricture" both in Chinese and English.Meta analysis or repetitive studies were excluded.After that,20 documents concerning the effects of materials and types of biliary tract stents on patency rates and patency times were analyzed.RESULTS AND CONCLUSION:Biliary stent implantation is the preferred way for alleviative treatment of malignant obstruction of bile duct with few complications.Biliary tract stents comprise two types comprise metallic stents and plastic stents,the biocompatibility of both stents need to be improved.The metallic stents exhibited advantages in preventing bacteria,maintaining stent patency and reducing complications,but the high cost limited its application.Though plastic stents easily lead to stent occlusion due to bacteria adhesion and biliary siltation,however,it has merits of easy change and cheap cost.The combination of biliary stent implantation and radiotherapy can prolong effective patency period and prevent stent occlusion.The results demonstrated that biliary stent implantation is best methods for treating malignant obstruction of bile duct,and combined biliary stent implantation with radiotherapy can prevent stent obstruction.

Objective To study the rule of breaking Ferula fukanensis seed dormancy under low-temperature and content changes of endo-hormone.Methods The seeds were treated with stratification under 4 ℃ and germinated under 20 ℃.The content of endo-hormone,such as Z,GA_3,IAA,and ABA,was mensurated by HPLC.Results The seed germination rate achieved as high as 14% in 20 d and more than 60% in 40 d.Among breaking the seed dormancy,the content of endo-hormone was decreased gradually,while the rate of GA_3 and ABA was increased quickly in 10—20 d under 4 ℃ stratification.ConclusionThe stratification under 4 ℃ could break the seed dormancy.The rate of GA_3 and ABA is a pivotal factor of the seed dormancy.The endo-hormones IAA and Z have the significant effect on seed germination.

Objective To assess the clinical significance of SAA, IP-10 and PCT in the diagnosis of AECOPD. Methods Sixty AECOPD patients, 52 with sCOPD, and 28 healthy subjects were assigned to three groups. Clinical data and serum specimen were obtained from another 19 AECOPD patients at stable stage as AECOPD-sCOPD group. Serum levels of SAA, IP10 and PCT were quantitatively measured by ELISA. Levels of multiple serum markers were statistically compared among different groups. Results The concentration of SAA significantly differed between the AECOPD and sCOPD groups (P < 0.05), so did the concentration of IP-10 (P < 0.05); no statistical significance was found in terms of serum PCT concentration between the two groups (P > 0.05). Conclusions As compared with the sCOPD group, levels of serum SAA and IP-10 in the AECOPD group were significantly elevated, which is helpful in the diagnosis of AECOPD with a sensitivity and specificity of 100% and 54.9%for SAA, and 96.1%and 75.0%for IP-10. However, PCT level failed to identify AECOPD from sCOPD.

BACKGROUND:In the repair of urinary tract defects, we have been actively trying to construct the urinary tract substitutes with normal physiological function through combining ideal seed cel s and proper scaffold materials by tissue engineering method. OBJECTIVE:To investigate the biocompatibility of bone marrow mesenchymal stem cel s with rabbit bladder acel ular matrix scaffold. METHODS:Rabbit bone marrow mesenchymal stem cel s were isolated and cultured using density gradient centrifugation method. Passage 3 rabbit bone marrow mesenchymal stem cel s were cultured on the rabbit bladder acel ular matrix. The cel s were counted every day for 12 days, to drawn a cel growth curve. Bone marrow mesenchymal stem cel s cultured alone were used as control group. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s were successful y seeded onto the bladder acel ular matrix. Under the inverted microscope, the cel s grew out of the bladder acel ular matrix, and a great amount of long spindle-shaped cel s were found around the bladder acel ular matrix. With 5 days of inoculation, the cel s in the two groups grew gently;at 6-9 days, the cel growth curve gradual y became steeper, and the cel division and growth were increased exponential y;at 10-12 days, the cel s recovered to a gentle state. Cel growth curves in the two groups were basical y coincident, suggesting that rabbit bone marrow mesenchymal stem cel s have good biocompatibility with the bladder matrix.

Nineteen patients with low rectal cancer underwent laparoscopy-assisted anterior resection with prolapsing technique for sphincter preservation.The clinical data were retrospectively reviewed.Fecal continence was preserved successfully in all patients.There was no perioperative mortality,only two cases with anastomotic leak and one case with local recurrences.Laparoscopy-assisted anterior resection with a prolapsing technique for low rectal cancer is safe procedure and can be an alternative sphincter-preserving operation.

0.05). PET/CT was more sensitive than B ultrasound and mammography in diagnosing axillary disease(P

Objective To analyze the diagnostic significance of the expression of perinuclear anti-neutrophil cytoplasmic antibody(pANCA) and anti-Saccharomyces cerevisiae antibody(ASCA) in the patients with inflammatory bowel disease(IBD).Methods Serum samples were collected from 97 patients with IBD,including 86 patients with ulcerative colitis(UC) and 11 with Crohn′s disease(CD).Another group of 36 persons in whom colonoscopic examination was negative served as control.Determinations of ASCA and pANCA were performed using indirect immunofluorescence test(IIFT).Results The positive rate of pANCA in group UC,CD and control was 27.9%,0% and 0%,respectively.Obviously,the rate in group UC was higher than that in the other two groups(P0.05).Four of 11 CD patients(36.4%) were severe cases,among them 3 showed ASCA+;7 were moderate cases(63.6%),and among them 1 case showed ASCA+.The positive ratio of ASCA was significantly different between severe and moderate CD cases(P

0.05).The DNA content in samples of ITS group was significantly lower than that of FBS group on day 6(P

OBJECIVE:To study the preparation technology of Psoralea corylifolia-Juglans regia tincture.METHODS: Taking Psoralea corylifolia and Juglans regia as raw material,which were extracted respectively with ethanol and mixed after having been condensed,then added with surface-active agent to prepare the compound preparation Psoralea corylifolia-Juglans regia tincture.RESULTS: The tincture was bright and uniformly sable in color.CONCLUSION: The preformulation and the preparation technology are both reasonable and the tincture is stable in quality.