Berberine is a taste of traditional Chinese medicine.With the progress of research on pharmacology and mechanism of drug action of extensive research,the new mechanism of berberine was revealed through a series of clinical trials,the clinical indications continue to expand,and berberine has a therapeutic effect in digestive and endocrine system disease.The current study confirmed that berberine not only has the broad-spectrum antibacterial activity,but also plays a strong anti-inflammatory and immune regulatory role,which makes more and more research began to concern its application value in the diseases of respiratory system.The strong anti-inflammatory effect of berberine cau effectively relieve the airway inflammation of patients who are suffering from chronic obstructive pulmonary,and the broad-spectrum antibacterial effect brought hope for the treatment of tuberculosis and pneumonia.The subsequent animal and clinical studies have confirmed that berberine can effectively improve the symptoms of respiratory system diseases,and play a role in the treatment.

Replacement therapy is the most effective method for the treatment of end-stage liver disease,and decellularized liver bioscaffold broadens the research field of the replacement therapy.The present liver bioscaffold preparation is to perfuse chemical reagents (detergents,enzymes,et al) into the vascular structure of the liver under certain physical conditions,so as to remove cellular components and retain extracellular matrix and microvascular structure.Cells were reseeded into the decellularized liver scaffold to obtain the recellularized liver,which can be cultured and evaluated in vitro or in vivo by observing the adhesion of seeded cells,detecting the synthesis and secretion of the recellularized liver.Currently,the selection of seed cells,recellularization protocol and recellularized liver transplantation are still under exploration.In this review,the preparation,evaluation,detection and application of the decellularized liver bioscaffold are introduced for the further experimental study and clinical research.

Objective To investigate the prevalence and related factors of peptic ulcer (PU) and Helicobacter pylori (Hp) infection in military men. Methods A stratified multi-stage cluster randomly sampling method was used. A questionary survey was conducted in 6 160 soldiers garrisoned at south China, inclding: PU symptoms and risk factors questionnaires; gastroscopy and Hp detection. Results Incidence of PU correlative symptoms (Sc≥5) in soldiers was 18.60%. The prevalence of PU was 12.78%.Higher incidence of PU was found among the soldiers who enlisted for less than 1 year, or were from south China, or served in armored troops. The prevalence of Hp infection was 62.67%. Hp infection (OR4.60), smoking (OR3.8), sustained intense training (OR4.3), fighting vehicle driving (OR6.0) and frequent mental intension (OR3.7) seemed to be the main risk factors of PU. While hot food, milk and whether or not the only child in a family showed no relation with PU incidence. Conclusion Higher prevalence of PU and Hp infection existed in service men. For soldiers, PU incidence is closely associated with Hp infection, military training, mental factors, and also related to soldiers′ enlisted period, arm of the service and the region where the soldiers come from.

Objective To evaluate the curative effect and indications of medical ozone for the treatment of lumbar disc herniation.Methods 10~20 ml(35~45 ug/ml) medical ozone was injected into the lumbar disc percutaneously each case. The patients were followed up for 6~12 months. Results 60 cases (66 lumbar discs) with lumbar disc herniation were treated with the injection of medical ozone. The total effective rate was 96.67% , excellent and good rate was 76%.The efficacy in herniation to be 30% of vertebral canal diameter and lumbardisc bulging group. Conclusion The treatment of lumbar disc herniation with medical ozone is simple,effective and no complications.

Objective To research pacemaker cells outside conductive system of rat and mouse heart. Methods Paraffin and frozen serial sections (stained with HE, Masson, cholinesterase technique), and ultrastructural sections, from atrial tissue of 10 rats and 15 mice were observed by light and electron microscopy to find latent pacemaker-like cells. Results Some round or elliptical cells were scattered and irregularly detected in right and left atrial tissue of rat and mouse. Their nuclei were larger, plasma was clearer and cellular organelles were fewer than those of working myocyte. These cells were similar to pacemaker cells in sinoatrial node.Conclusion There are some pacemaker-like cells in right and left atrial tissue of rat and mouse. The finding can provide a morphological basis for ectopic beat of atrium.

The salivary glands were exposed and isolated from the larvae of Simulium quinquestriatum and stained in carbol fuchsin, squashed between slide and coverslide. Slides were examined and photographed under microscope to measure the polytene chromosomes. Systematic analysis was made. Results indicated that the number of the polytene chromosomes of both isolates is three. The main characteristic chromosomal structures are homologized. Only the banding types of ⅡL are different.

Objective To investigate MRI signal features and MRI appearances of patients with advanced stage in polyacrylamide hydrophilic gel injection for facial plasty.Methods In this study,MRI of 11 cases with 23 polyacrylamide hydrophilic gel injection of facial plasty for 6 to 10 years were retrospectively reviewed.All images were acquired with GE 3.0T MR imaging unit.MR sequences,including FSE T1WI,FSE FS T2WI,and STIR were applied with 8-channel brain coil.MRIs sliced through the maxillofacial region in the transverse,coronal and sagittal planes.Results In 11 cases of 23 polyacrylamide hydrophilic gel injection,there were different degrees of capsule rupture and induration in 6 polyacrylamide hydrophilic gel injection,and the images showed sporadic callosities such as subcutaneous nodules and nodules in glands or muscles ; hydrogel migration in 8 polyacrylamide hydrophilic gel injection.Secondary deformity occured in 80 ％ cases,in which the most cases were induced by hydrogel migration.Conclusions Magnetic resonance imaging can make clear of the type of rupture and the distribution leakage of polyacrylamide hydrophilic gel for facial plasty and it is an ideal approach for advanced patients with polyacrylamide hydrophilic gel injection for follow-up.

Innate lymphoid cell is a type of newly discovered innate immune cell , which can function as adaptive T cells . Based on the transcription factors and the expression of effector cytokines , innate lymphoid cells can be categorized into three sub-groups:ILC1, ILC2 and ILC3.The inflammatory factors these produced by ILC play an important role in the development of inflamma -tory bowel disease (IBD), but ILC3 can secrete IL-22, which promotes the restoration of intestinal epithelial cells .Exploring the func-tion of ILC has a great significance in the treatments of IBD .This article reviews their phenotype , function and also relationship with IBD.

Objective To evaluate the performance of parallel test in detecting malaria infection for returned person from malaria endemic area.Methods The blood samples of 484 returnees from malaria endemic area were analyzed and detected by thick blood smear,rapid diagnostic test (RDT) and nest PCR in four companies involving the African labor dispatching.Results The sensitivi ty of thick blood smear and RDT was 0.628 and 0.744 respectively,which of the parallel test was 0.930.On the other hand,the area under the curve (AUC) of parallel test was 0.930 (95％CI:0.895-0.986),which was higher than thick blood smear[0.814 (95％CI:0.724-0.904)]and RDT[0.847 (95％CI:0.769-0.926)].Conclusion Thick blood smear and RDT,which consist of parallel test,could improve the detection sensitivity and accuracy for returnees from malaria epidemical area effectively.This approach is worthy of popularization and application.

Objective To review advance of gabapentin in treatment of refractory chronic cough, and to provide evidence for its clinical usage and further study.The original articles referring to gabapentin’ s effect on sensory neuropathy such as refractory chronic cough, which were retrieved from CNKI, Wanfang Medical Network, as well as PubMed over the last 15 years, were reviewed.The safety, efficacy and its mechanism of gabapentin were sorted, generalized and analyzed.Gabapentin appears to be effective and safe in the treatment of sensory neuropathic disorder such as refractory chronic cough, and its effective treatment results may come ture through improving central sensitization, which indicates the drug has new clinical application value.Relevant clinical trials investigating its efficacy and safety profile in the treatment of cough are limited and further research are needed.