Sinusoid lining endothelial cell(SEC) of the liver is a group of cells different from vessel lining endothelial cells with high heterogeneity characteristics, it shows many biological functions in normal liver and plays a role in liver injury caused by acute and chronic liver diseases as well as fibrogenesis. Increasing attention has been paid to the capillarization in hepatocellular carcinoma in recently years, some studies showed that SEC is attributed to the formation of capillarization with changes of the immunophenotype, such as expression of CD34.The pathological study of SEC on liver disease was briefly reviewed in this paper.

Objective To study the rule of breaking Ferula fukanensis seed dormancy under low-temperature and content changes of endo-hormone.Methods The seeds were treated with stratification under 4 ℃ and germinated under 20 ℃.The content of endo-hormone,such as Z,GA_3,IAA,and ABA,was mensurated by HPLC.Results The seed germination rate achieved as high as 14% in 20 d and more than 60% in 40 d.Among breaking the seed dormancy,the content of endo-hormone was decreased gradually,while the rate of GA_3 and ABA was increased quickly in 10—20 d under 4 ℃ stratification.ConclusionThe stratification under 4 ℃ could break the seed dormancy.The rate of GA_3 and ABA is a pivotal factor of the seed dormancy.The endo-hormones IAA and Z have the significant effect on seed germination.

Papillary carcinoma is one of the commonest tumors in the thyroid.The pathologic diagnosis of papillary carcinoma as well as follicular carcinoma and follicular adenoma of the thyroid are based on the morphology observation hitherto.However,the diagnostic value of immunostaining for distinction among differentiated neoplasm of the thyroid was so far absent.With the identification of the special markers of lymphatic vessel endothelial cell by immunohistochemical method in the recent years,the rapid progress in the research of lymphangiogenesis of the tumors had been made.This article briefly reviewed the progress of the special markers of lymphatic vessel endothelial cell and vascular endothelial growth factors in the differentiated neoplasm of the thyroid.

Objective To discuss anatomic landmarks during retroperitoneoscopic radical nephrectomy. Methods A total of 46 patients underwent retroperitoneoscopic radical nephrectomies from November 2002 to July 2004.The first trocar was introduced at the union of the site 2 cm below the 12 th costal margin with the lateral side of the musculus sacrospinalis.The second trocar was placed at the midpoint of the upper border of the iliac crest,or at the location above and medial to the anterior superior iliac spine.And the third one was inserted at the union of the anterior axillary line with the costal margin.In the first place,the greater psoas muscle was identified as the dorsal marker.Then the diaphragmatic muscle was identified as the posterior marker of the upper pole of the kidney,and the adrenal gland as the medial marker of the upper pole of the kidney,and the peritoneum as the ventral marker.The renal vein lay below and anterior to the renal artery.The right renal vein was routinely sperated right up to the joining site with the inferior vena cava.A linear cutter was used to resect the renal vein and artery respectively. Results The mean operation time was 145 min (range, 60~ 255 min) and the mean intraoperative blood loss was 133 ml (range, 20~1 000 ml). Adrenalectomy was performed in 32 patients (69 6%). A conversion to open surgery was required in 1 patient because anatomic landmarks could not be well seen. The inferior vena cava was wrongly closed off by 1/2 cross-section when using a linear cutter to sever the right renal vein in 1 patient. Peritoneal injuries happened in 4 patients.Follow-up was carried out for 1~20 months (mean,9 months) in all the 46 patients.One patient died of liver metastasis 1 year after the surgery, while the remaining 45 patients survived without recurrence. Conclusions Proper identification of anatomic landmarks is crucial to a successful retroperitoneoscopic nephrectomy.

2-year-old cutting seedlings of Taxus cuspidata have a three- month-growth period fromMay to July and its peak occures in June. The experiment showed that 18% of light permeability and 17% of soil moisture are most suitable conditions.

Two peaks were present in the NRA annual curve of Panax quinquefolius. A high peak appeared in late April and a smaller peak occured in early July. It is important to apply N2 fertilizers just before the appearance of the two peaks. Experiment on the effect of different light and pH showed that higher NRA were formed at 4000 Lx and pH 4.3～6.0.

443) ?mol/L) due to upper urinary calculous obstruction,urgently treated by pneumatic lithotripsy(EMS,Switzerland) under ureteroscopy,from August 2002 to April 2006 in this hospital.After stone fragmentation and removal,an indwelling double-J stent was placed into the ureter at the same time.Results The continuity of the ureter was restored after one session of lithotripsy in all the 9 cases(14 sides).The ureteral calculi were thoroughly removed on one session in 7 cases(12 sides).Few residual stones were found in the renal pelvis in 2 cases(2 sides),in one of which the stones were spontaneously expelled after oral medication,and in another,after extracorporeal shock wave lithotripsy(ESWL).No severe complications happened,such as ureteral avulsion,rupture,or perforation.Concentrations of serum creatine were decreased to normal levels within 3~7 days after operation in all the 9 cases.The gross blood urine disappeared in 1~4 days postoperatively.The patients were discharged from the hospital after 3~7 days (mean,5 days) after operation.The double-J catheter was removed at 1~2 months postoperatively.Follow-up examinations in 7 cases for 2~30 months(mean,14 months) found normal levels of serum creatine and no recurrent urinary stones under B-ultrasonography or X-ray radiography.Conclusions Ureteroscopic pneumatic lithotripsy in the treatment of acute renal failure due to upper urinary calculous obstruction is an effective treatment with short operation time,safety,and minimal trauma.By using this procedure,both sides of ureteral stones can be removed on one session.

Objective To investigate the clinical characteristics and therapy methods of dorsolateral medullary syndrome.Methods The clinical data of 36 cases of dorsolateral medullary syndrome were analyzed retrospectively.Results The palients presented with acute or sub-acute oneset.Vertigo(83.3%),dysarthria(61.1%),dysphagia(52.8%),Horner's syndrome(80.6%),ataxia(72.2%) and crossed sensory disturbance(50%) were the most common symptoms and signs.MRI examination demonstrated dorsolateral medullary infarction in 32 of 36 patients.13 patients received DSA examination and the results showed 6 patients with different degree disease of vertebral arery,2 patients with isolated posterior inferior cerebellar artery occlusion,1 patient with vertebral occlusion and ipsilateral posterior inferior cerebellar artery stenosis.In 33 patients who received anticoagulation,antiplatelet and activating blood circulation to dissipate blood stasis therapies,26 patients improved 7~10 days after treatments and the symptoms almost disappeared during 1～2 months.6 cases remained different degree sensory disturbance and ataxia 1 case died.3 patients were treated with percutaneous transluminal angioplasty and stenting.The symptoms relieved at the day of operation and recovered completely 1 week after operation.Conclusions Dorsolateral medullary syndrome is a clinical syndrome because of insufficient blood-supply in local blood vessel.MRI is sensitive for the diagnosis of dorsolateral medullary syndrome.The location and degree of the disease can be identified by DSA.Intervention treatment is an effective method in the therapy of dorsolateral medullary syndrome.