ObjectiveTo compare the sensitivity among three diagnostic criteria for multiple myeloma.MethodsA total of 220 patients with multiple myeloma were studied retrospectively to compare thesensitivity, aswellastheirclinicalmanifestations, cellmorphologyinbonemarrowand immunophenotype.Results (1) The sensitivity of domestic diagnostic criterion in 1975 was 79.1％ (174/220) and there were some drawbacks in type identify.The sensitivity of WHO diagnostic criterion in 2001 was 97.3％ (214/220) and the sensitivity of domestic diagnostic criteria in 2011 was 100％. (2) Immunoglobulin level in 12.7％ (28/220) patients was ＜ 30 g/L,plasma cells count in bone marrow in 13.6％ (30/220) was ＜ 10％ ; 2.7％ (6/220) patients had not met the standard in immunity globulin and bone marrow plasma cells count.(3) The immunophenotype was CD38 positive ( 100％ ),restricted light chain (kappa/lambda) and CD19 100％,CD138 (98.2％,216/220) negative.ConclusionsAmong the three diagnostic criteria,the highest was 2011 domestic diagnostic criteria.Comprehensive analysis include clinical manifestations,cell morphology,immunophenotype will contribute to the diagnosis for multiple myeloma.

Objective To investigate the effect of TGF? 1 on expression of MHCⅠ type antigen in human pulmonary squamous cell carcinoma.Methods Expressions of ? 2 M in primary culture cells with rhINF? and rhTGF? 1+rhINF? from human pulmonary squamous cell carcinoma were detected by FCM(Flow Cytometry Method), respectively. Results Expression of ? 2 M in all tumor cell groups was induced significantly by rhINF? ( P 0.05) Conclusion Expression of INF? induced MHCⅠ type antigen in human pulmonary squamous cell carcinoma can be inhibited by TGF? 1.

Objective To investigate the expression of TGF ? 1 in human pulmonary squamous cell carcinoma Methods TGF ? 1 in serum free conditioned medium of different differentiated degree primary cells in culture from human pulmonary squamous cell carcinoma were detected by ELISA Results TGF ? 1 in serum free conditioned medium from all tumor cell groups is significantly higher than from normal bronchial epithelial cell groups ( P 0 05) Conclusion Inhibition effect by TGF ? 1 on cell growth of human pulmonary squamous cell carcinoma is weakened

The value of dobutamine 99m橳c-MIBI SPECT (DBA-SPECT) for detecting coronary artery disease was assessed in 56 patients with chest pain. Exercise ECG, DBA-ECG and DBA-SPECT were obtained. The hemodynamic changes in DBA infusion are similar to that in exercise test. In 21 patients who had coronary angiogram,the sensitivity,specificity and accuracy were 87. 5% ,76. 9% ,81. 7% ,respectively in DBA-SPECT;75. 0%, 92. 3%, 85. 0%, respectively in DBA-ECG,and 87. 5%,46. 2% ,61.9% ,respectively in exercise ECG test. The results showed that DBA-SPECT is a feasible safe and non-invasive method for detecting coronary artery disease.

Objective To observe the health-related quality of life of children with recurrent respiratory tract infection (RRTI) simultaneously inoculated by pneumococcal vaccine combined with influenza vaccine.Methods A total of 454 cases with RRTI were randomly divided into four groups,the control group (n =135) was used with placebo only,and the combined inoculation group (n =143) was simultaneously inoculated with pneumococcal vaccine combined with influenza vaccine,the pneumococcus vaccine group (n =95),influenza vaccine group (n =81).The PedsQLTM 4.0 score and clinical indicators were observed in each group before the inoculation and 14 months after the inoculation.Results There were no significant differences in the number of cases,gender,age,duration and socioeconomic status score in the four groups (P ＞ 0.05).The incidence of upper and lower respiratory tract infection,respiratory diseases caused by the frequency of fever,the number of days of antibiotic use and total cost of treatment in the vaccine group were significantly less than those of the control group (P ＜ 0.05).And the combined inoculation group was significantly better than the pneumococcus vaccine group and influenza vaccine group(P ＜ 0.0l).The PedsQLTM 4.0 score showed no significant differences among four groups (P ＞ 0.05) before the inoculation.Each dimension of the quality of life of the vaccine group significantly increased (P ＜ 0.05) while those of control group showed no significant difference (P ＞ 0.05).Conclusion Combination of inoculation of pneumococcal vaccine and influenza vaccine can effectively improve the health-related quality of life of RRTI children as well as the PedsQLTM 4.0 score,and it should be highly recommended clinically.

Objective: To study the therapeutic method of treating hypertensive cerebral hemo rrhage and to evaluate the curative effects.Methods: Thirtynine cases (A group) with hypertensive cerebral hemorrhage wer e treated wit h therapeutic method of internal medicine and other 40 cases (B group) treated with internal medicine combined with traditional Chinese herbs fed via gastric tube plus drawing hematoma through skull pun cture.Results:The cured rate (32.5%) and effective rate (90.0%) in B group were signific antly higher than those in A group (10.3％ and 46.2％ respectively) meanwhil e the complete obsorbsion and clearan ce time of hematoma were significantly shorter,and the complications were lesse r.Conclusions:The therapeutic method of traditional Chinese herb fed via gastro tube combined with drawing through skull puncture for treating hypertensive cere bral bleeding is easy to manipulate and worth spreading.

ObjectiveTo evaluate the efficacy and toxicity in local regionally advanced nasopharyngeal carcinoma(NPC) patients treated by cetuximab combined with intensity modulated radiation therapy.Methods58 cases of untreated NPC patients were randomly divided into treatment group and control group.The treatment group was given the treatment of cetuximab combined with intensity modulated radiation therapy,the control group was given the treatment of intensity modulated radiation therapy.The short-term efficacy and toxicity were evaluated,followed up for 1-year the objective response was evaluated.ResultsAfter treatment and 3 months after treatment,the CR rate of the nasopharynx and cervical lymph node of the treatment group were higher than the control group,compared with control group the difference was significant(P ＜0.05) ;Follow-up rate was 100％,the median follow-up time was 12 months,all patients were no deaths in the follow-up period; the local control rate of the treatment group was 89.3％,it was higher than the control group(73.3％ ),compared with control group the difference was significant( P ＜0.05) ;The distant metastasis rate of the treatment group was 3.6％,it was lower than the control group(23.3 ％ ),compared with control group the difference was significant( P ＜0.05) ;Most acute toxicity of the two groups were 1 to 2 degrees,and the difference was not statistically significant( P ＞ 0.05 ) ; During the treatment,28 patients of the treatment group occurred acne-like rash,after symptomatic treatment and nutritional support therapy was initiated.ConclusionThe cetuximab combined with intensity modulated radiation therapy in treatment of local regionally advanced nasopharyngeal carcinoma had better efficacy and lower toxicity,the therapy method was worthy to be popularized.

Objective: To investigate the clinical and pathological features of solid-pseudopapillary neoplasm of pancreas with emphasis on its diagnosis and differential diagnosis.Methods:Clinical data,pathological features and immunohistochemical findings were collected and a review of the literature was made,differential diagnosis was discussed in the cases of SPNP. Results:All the 5 patients were females,age from 16 to 65 years(average 32 years),there were no recurrence after tumor resection.The mean diameter of tumors was 8 cm,all encapsulated,and solid with cystic changes.Histological examination showed presence of solid sheets,Psudopapillary with uniform population of cells.The nuclei were oval,round without atypia,and mitotic figures were rare.Psudopapillary structure with fibrovascular core was remarkable.Hemorrhage,foamy cells and cholesterol crystals were often found.Immunohistochemically,5 cases were positive for ?1-ACT and Vimentin,3 cases expressed NSE and Syn,2 cases expressed CgA and CK.All cases were negative for Insulin and EMA.Conclusion:SPNP is a rare pancreatic neoplasm.Histologically,uniform population of cells formed psudopapillary structure with a fibrovascular core,and it is a clinicopathologic entity in young female patients with a benign clinical course.

Objective To evaluate the efficacy of ureteroscopic pneumatic lithotripsy for ureteric calculi.Methods From January 2003 to June 2007,221 patients with ureteric calculi underwent ureteroscopic pneumatic lithotripsy in our hospital.Under continuous epidural anesthesia combined with spinal anesthesia,the surgery was performed in lateral lithotomy position.Double-J catheter was used for drainage after the operation.Results The mean operation time was 55 minutes(ranged from 15 to 118 minutes).Among the patients,the calculi were found in the upper ureter in 16 cases,middle segment in 52 cases,and lower ureter in 153 cases.A total of 228 stones in the 211 patients were all fragmented with a success rate of 100%.The discharging rate was 95.5%(211/221).ESWL was used in 7 cases with unsatisfied outcomes and 3 patients who had ipsilateral renal calculi.A 3-to 6-month follow-up was achieved in 189 of the patients,who had no recurrence during the period.Conclusions Ureteroscopic pneumatic lithotripsy is a minimal invasive and effective treatment for ureteric calculi.Postoperative ESWL is an alternative which can increase the rate of stone clearance.

Objective To evaluate the morphological features and distribution of Cajal-like cells in the human upper urinary tract after the laparoscopic nephrectomy.MethodsFrom January 2008 to August 2008,specimens of the kidney were obtained from 23 patients after laparoscopic nephrectomy in our hospital.Each specimen included the renal calyces,renal pelvic,and proximal ureter.HE staining and CD117 immunohistochemical staining were performed.The Cajal cells harvested from normal colon were used as positive control.The cells were observed under light microscope and the distribution of the Cajal-like cells in the upper urinary tract were analyzed.ResultsThe morphology of Cajal-like cells in the human upper urinary tract were similar to those collected from the colon,both showed fusiform shape and were positive for CD117.The Cajal-like cells within the colon were located surrounding the myenteric nerve plexus between the smooth muscles,whereas the Cajal-like cells distributed in the human upper urinary tract were diffused within the lamina propria and muscles.The density of Cajal-like cells at the renal calyces,renal pelvic,and proximal ureter was 15.4?5.4/cm2,22.6?6.6/cm2,and 19.9?5.8/cm2,respectively.The density at the renal calyces was significantly lower than those in the renal pelvic and proximal ureter(P=0.000,P=0.014),whereas,no significant difference was found in density between the renal pelvic and proximal ureter(P=0.129).ConclusionsCajal-like cells exist in the human upper urinary tract with different distribution characteristics from those in the gastrointestinal tract.In the upper urinary tract,the density of the cells is various,which may be related to the functions of the different parts of the tract.