With the emergence of new concepts, methods and techniques in modern medicine, the basic research and clinical treatment for gynecological oncology has rapidly developed in the past couple of years. Evidence-based medicine, individualized therapy and microinvasive therapy give us more opportunities to treat the patient. Nowadays, it is possible for the clinic to implement new approaches to improve the outcome and the quality of life for the patients with minimal side effects at the same time. There has been a lot of reports from in vitro studies suggesting that gonadotrophin may play an important role in tumorigenesis, estrogen may promote tumor cell proliferation through PAX2 gene. Survivin, a protein serving as an apoptosis inhibitor, may be crucial for the regulation of tumor cell proliferation and apoptosis.

ObjectiveTo evaluate the efficacy and toxicity in local regionally advanced nasopharyngeal carcinoma(NPC) patients treated by cetuximab combined with intensity modulated radiation therapy.Methods58 cases of untreated NPC patients were randomly divided into treatment group and control group.The treatment group was given the treatment of cetuximab combined with intensity modulated radiation therapy,the control group was given the treatment of intensity modulated radiation therapy.The short-term efficacy and toxicity were evaluated,followed up for 1-year the objective response was evaluated.ResultsAfter treatment and 3 months after treatment,the CR rate of the nasopharynx and cervical lymph node of the treatment group were higher than the control group,compared with control group the difference was significant(P ＜0.05) ;Follow-up rate was 100％,the median follow-up time was 12 months,all patients were no deaths in the follow-up period; the local control rate of the treatment group was 89.3％,it was higher than the control group(73.3％ ),compared with control group the difference was significant( P ＜0.05) ;The distant metastasis rate of the treatment group was 3.6％,it was lower than the control group(23.3 ％ ),compared with control group the difference was significant( P ＜0.05) ;Most acute toxicity of the two groups were 1 to 2 degrees,and the difference was not statistically significant( P ＞ 0.05 ) ; During the treatment,28 patients of the treatment group occurred acne-like rash,after symptomatic treatment and nutritional support therapy was initiated.ConclusionThe cetuximab combined with intensity modulated radiation therapy in treatment of local regionally advanced nasopharyngeal carcinoma had better efficacy and lower toxicity,the therapy method was worthy to be popularized.

Replacement therapy is the most effective method for the treatment of end-stage liver disease,and decellularized liver bioscaffold broadens the research field of the replacement therapy.The present liver bioscaffold preparation is to perfuse chemical reagents (detergents,enzymes,et al) into the vascular structure of the liver under certain physical conditions,so as to remove cellular components and retain extracellular matrix and microvascular structure.Cells were reseeded into the decellularized liver scaffold to obtain the recellularized liver,which can be cultured and evaluated in vitro or in vivo by observing the adhesion of seeded cells,detecting the synthesis and secretion of the recellularized liver.Currently,the selection of seed cells,recellularization protocol and recellularized liver transplantation are still under exploration.In this review,the preparation,evaluation,detection and application of the decellularized liver bioscaffold are introduced for the further experimental study and clinical research.

This paper introduces the surgeon backpack and medical corpsman backpack which are newly developed for PLA.The twokinds of backpacks being waterproof,water can't penetrate intothem when they are in the rain or floating on water.The basic performance experiments,ergonomics tests and army tryout are performed.The results show that the backpacks are designed reasonably and easy touse.The burthen on the whole backpacks is distributed in the equipment uniformly due toits structure.The physical energy consumption of the person carrying the backpacks decreases due tothe same reason.The medical equipments in the backpacks can accomplish the first aid operations such as binding up,stanching,acesodyne,fastness,ventilation,infection prevention and anti-shock.

Objective To investigate the effect of MOTOmed movement therapy on balance and ability in the activities of daily living (ADL) of convalescing stroke patients with hemiplegia. Methods Forty convalescent stroke patients exhibiting hemiplegia were randomly divided into a treatment group and a control group with 20 cases in each group. The control group received routine rehabilitation training; the treatment group received MOTOmed training in addition. Balance function of all the patients was assessed using Berg's balance scale (BBS) , and the Barthel Index ( BI) was used to assess ADL ability at the beginning of the program and after 6 weeks of treatment. Results Balance and ADL ability improved significantly in both groups after 6 weeks of treatment. The effect in the treatment group was significantly better than in the control group. Balance and ADL ability were positively correlated. Conclusions Applying MOTOmed therapy along with routine rehabilitation training can distinctly improve balance and the ADL ability of hemiplegics after stroke.

Abstract: Objective　To investigate the effects and mechanisms of resveratrol on in vitro growth and biofilm formation of hypermucinous Klebsiella pneumoniae(HMKP), so as to provide a preliminary theoretical basis for the development of new antimicrobial drugs. Methods Clinical isolates of Klebsiella pneumoniae, non-repetitive, collected from March to October 2023, were identified for species and sensitivity to common antimicrobials using the VITEK-2 compact automatic microbial identification system. The mucous characteristics of the strains were assessed via a string test, and the broth microdilution method determined the minimum inhibitory concentration (MIC) of resveratrol. The effects of different concentrations of resveratrol on the growth of hypermucinous Klebsiella pneumoniae were observed by the bacterial growth curve method, and the effects of different concentrations of resveratrol on HMKP biofilm were determined by the crystal violet staining method. Real-time quantitative reverse transcription PCR (RT-qPCR) was used to detect the expression levels of virulence-related genes (aerobactin, rmpA, and mrkD) in HMKP. Data plotting and analysis were performed using GraphPad Prism 9.0. Results　A total of 122 strains of Klebsiella pneumoniae were collected, with 29 strains testing positive in the string test (HMKP). Among these, 26 strains were allergic to commonly antimicrobial drugs, such as cephalosporins and their enzyme inhibitors, quinolones, and aminoglycosides, while 3 strains were identified as carbapenem-resistant hypermucinous Klebsiella pneumoniae (HM-CRKP). The minimum inhibitory concentration (MIC) of resveratrol for all HMKP strains exceeded 512 μg/mL. Resveratrol concentrations of 256 μg/mL and 128 μg/mL significantly reduced the growth capacity and biofilm formation capacity of HMKP (P<0.05) and decreased the expression levels of virulence-related genes rmpA and mrkD. Conclusions　Resveratrol can inhibit the growth and biofilm formation of HMKP. The mechanism may be related to the downregulation of virulence-related genes expression, specifically rmpA and mrkD.

BACKGROUND:Due to the puncture and difficult reduction, severe vertebral compressive fracture is considered to be the relative contraindication of vertebroplasty and kyphoplasty. OBJECTIVE:To investigate the vertebra reduction after the treatment of severe vertebral compressive fractures with percutaneous kyphoplasty. METHODS:Thirty patients (42 vertebral bodies) with severe compressive fractures were included. The compressive rate of the affected vertebral bodies was 75%-83%. The direction of percutaneous puncture was guided under digital subtraction angiography, and then postural reduction and local kyphoplasty were performed. After satisfactory reduction, bone cement was injected. RESULTS AND CONCLUSION:Al the 30 patients tolerated procedure wel . The mean height of the anterior, mid and posterior vertebral body was improved from (0.48±0.17) cm, (0.83±0.23) cm and (2.44±0.33) cm preoperatively to (0.71±0.22) cm, (1.21±0.25) cm and (2.44±0.33) cm respectively after operation. The postoperative height of the anterior, mid and posterior vertebral body was significantly higher than that before operation. The visual analogue scale score after treatment was significantly lower than that before operation. The results indicate that percutaneous kyphoplasty can ease pain, restore vertebral body height and improve quality of life of the patients with severe vertebral compressive fractures.

This study is to investigate the protective effect of astaxanthin against injured hepatocyte L-02 cells induced by sodium azide (NaN3) and reveal the possible mechanisms. Hepatocyte L-02 cells were exposed to 100 mmol.L-1 NaN3 with various concentrations of astaxanthin pre-incubated, then the cell viability was measured by MTT method; The level of reactive oxygen species (ROS) was determined by DCFH-DA method; The changes of mitochondrial membrane potential (MMP) and apoptosis ratio were detected by JC-1 method and Annexin V-FITC/PI double stain method, respectively. Results showed that after cells were exposed to 100 mmol.L-1 NaN3 for 3 hours, the cell viability significantly decreased; ROS level and the percentage of late phase apoptosis increased obviously; MMP was also declined. When cells were pretreated with astaxanthin, the cell damage and late phase apoptosis ratio reduced and MMP was maintained. However, the level of ROS showed insignificant decrease (P>0.05). The beneficial concentration of astaxanthin in improving cell viability and MMP was not in a dose dependent manner and the most effective of which was 0.10 nmol.L-1 (P<0.01). In order to reveal its possible non-antioxidant mechanism, mitochondrial membrane was imitated and H+ transferring function of astaxanthin was also detected by bilayer lipid membrane (BLM) method. Results showed that 2.0% astaxanthin could transfer H+ efficiently. These suggested the mechanisms of astaxanthin in protection of hepatocyte L-02 cells not via its ROS quenching capability but via its H+ transferring function, which improved the mitochondrial function and had the sequence biology effects.

Objective To assess whether topiramate prevents body weight gain in patients with olanzapine .Methods The ran‐domized controlled trials(RCTs) about topiramate for prevention of weight gain with olanzapine from 1998 to 2013 were searched in the Cochrane Library ,Pubmed ,EMbase ,WanFang Data ,CNKI and VIP .Two reviewers independently screened the literatures ,ex‐tracted the data ,and evaluated the methodological quality .Then Meta‐analyses were conducted by using RevMan 5 .1 software .Re‐sults The total of 11 RCTs were included .Among the 549 patients were involved .The results of Meta‐analyses showed that the ef‐ficacy of the topiramate group was superior to that of the control group in lessen body mass with significant difference (MD= -3 .68 ,95% CI:-5 .16- -2 .19 ,Z=4 .86 ,P<0 .01) .Conclusion Topiramate addition therapy is effective in attenuating olanzapine‐induced weight gain .

Purpose To investigate the clinicopathological changes of the diffuse-type giant cell tumor of the bone and joint. Methods 42 cases of the diffuse-type giant cell tumor of the bone and joint were studied and the follow-up data were reviewed. Results The male patients were 19 and the female were 23, with the male to female ratio of 1 ∶ 1. 2. The age of the patients was 8~69 years (the average age was 37. 6). It was displayed that the patients presented local pain and swelling (34 cases), including dysfunction (18 ca-ses) of the bone and joint. Clinically, the lesions located on the knee joint ( 71. 4%) in 30 cases, the hip and ankle in 9 cases (21. 4%), and the wrist elbow in 3 cases (7. 1%). 27 cases were examined by MRI. Among them, the diffuse-type giant cell tumor was diagnosed in 9 cases (33. 3%). The effusive lesions of the joint presented in 5 cases, the non-specific synovial lesions and others in 3 cases. The obviously diffuse hyperplasia of synovial cells with nodular growth pattern was found. However, a high proliferative in-dex of the tumor cells and the rich-cell tumor were found. But there was no tumor necrosis. Histopathologically, the tumor cells of dif-fuse-type giant cell tumor contained marked brown pigments, multinucleated giant cells formation and a lot of lymphocytes proliferation or follicular reaction in 17 cases (40. 5%) with invasive growth and involvement of the joint and surrounding tissue. 6 cases recurred (20%) , including 2 cases with 2 times. Conclusions The diffuse type-giant cell tumors most commonly involve the knee, followed by the hip and ankle. Preoperative examination of MRI can be help for the diagnosis of the tumor. Histopathologically, diffuse-type gi-ant cell tumor with synovial cells rich-hyperplasia and the villous structures formation more likely recur. The main biological character-istics of diffuse-type giant cell tumor are invasive growth pattern, usually into the soft tissue around the joints.