Since the 1990s,models of genetically modified mice(gene knockout,mutation or transgene overexpression) have been increasingly used in heart research.The findings from this class of murine models have contributed significantly to a number of research breakthroughs achieved in the last 15 years.Currently,the number of genetically modified mouse strains has been increasing steadily.These models that targeted a family or a functionally relevant group of proteins have formed systemic tools for research use.The database of mouse cardiac phenotype has been established.Meanwhile,the research methodology and strategic approach exploring mouse cardiac phentypes at molecular to in vivo levels have been mature.It is expected that the use of this class of genetically targeted mouse models will become increasingly important in future research.

Fluorescence in situ hybridization (FISH) is a non-radioactive technique by use of fluorescently labeled DNA probes in detecting chromosomal alterations in cells. As a new molecular cytological method, it can detect various types of cytogenetic alterations, including deletion, amplification and translocation, as well as improve the accuracy of pathological diagnosis if combined with histomorphology. This review focuses on the application of FISH to the detection of some solid tumors.

Objectives To observe anatomical location of the top branch of plantaris arch which extends from the superficial branch of the medial plantar artery,design and apply flap base on it. Methods On the basis of the anatomical data from 8 feet of 4 fresh cadavers and probed of the Doppler from 50 feet of normal person,defined the anatomic location and body surface projection of the top branch of plantaris arch,a type of new flap design were introduced in the paper. Results The flaps have been used in six cases clinically to treat skin defect of the hand meanwhile,sensation of the flap was regained by suturing nerve.All flaps survived,the appearance of the flaps were excellent,the function of the injured hands were recovered well. Conclusions The design of the flap is reasonable,it is suitable that the free medialis pedis flap of the top branch of plantaris arch is used to repair a small skin defect of the hand.

Following a brief introduction of relaxin family members, receptors and related signalling pathways,we review effects of relaxin on cardiovascular system,particularly,its anti-fibrotic action.The heart and vessels are target organs of relaxin.Recent studies have documented that the relaxin genes are upregulated in the diseased heart of humans and experimental animals.In several animal disease models,relaxin can reverse cardiac fibrosis.Relaxin may also indirectly promote myocardial regeneration and repair by suppressing fibrotic healing.Further research needs to focus on elucidating the exact mechanisms utilized by relaxin to induce these cardiac actions and translation of experimental findings to novel therapy of cardiovascular disease.

Objective MicroRNAs (miRNAs) are of important clinical value in various tumors.However, few studies are reported about their role in thymic epithelial tumors.This article aims to explore differential expression profile of miRNAs in type B3 thymoma and thymic carcinoma.Methods This study included the pathological data about 45 cases of type B3 thymoma and thymic carcinoma surgically treated in our hospital from January 2012 to January 2015, of which 3 cases of type B3 thymoma (control group) and another 3 cases of thymic carcinoma (case group) were subjected to miRNA microarray for determination of the differential expressions of miRNAs in the tumor tissues.The up-and down-regulated miRNAs were calculated, their target genes were predicted via online databases, and the thymus-related genes were identified.Results Totally, 32 differentially expressed miRNAs (including miR-125b-1-3p, miR-3175, and miR-4462) were up-regulated and another 19 (including miR-361-5p, miR-130a-3p, and miR-3651) down-regulated in thymic carcinoma.AKT1, C9, CD19, CDC42, LSS, and MYC were identified as the target genes of miR-377-5p, ADCYAP1R1, ASPA, CAD, and CD63 as the target genes of miR-458-5p, and AKAP12, CD28, FOXP1, and MDM4 as the target genes of miR-183-5p.Conclusion Differentially expressed miRNAs were identified in type B3 thymoma and thymic carcinoma and their target genes predicted using the prediction software, which may provide some valid evidence for further study of thymic epithelial tumors.

[Objective]To research the efficient way of isolating and proliferating of induced endothelial cells(ECs) ,measure the number of the ECs and observe the main biological characteristic of ECs m vitro during the passage cultivation.[Method]The marrow mononuclear cells(MNCs) in rabbit marrow were isolated by the gradiem centrifugation and cultivated to isolate and induce ECs. The efficiency of isolating and harvesting ECs was measured by the number of the first passage ECs from every 10~6 of the MNCs. The changes of ECs morphology, growth and proliferation were observed during the passage cultivation. The immunohistochemical staining of CD31 and vWF and function of ECs were checked.[Result]After cultivated at 1?10~6/cm~2 MNCs for 7～8 d, the first passage ECs were harvested. The number of the first and the second passage ECs had a remarkable statistical positive correlation with the number of the MNCs from one rabbit. After cultivated for 7～8 d, the first passage ECs of 7.086?0.75?10~4 in number and the second passage ECs of 53.20?6.47?10~4 in number were harvested from every 10~6 MNCs on average;ECs, which were monolayer arrayed to form cobblelike shape,The cells of 2nd passage showed positive staining of CD31 and vWF, and positive labeling of DiI-LDL and UEA.[Conclusion]The period from cultivating to harvesting ECs is shortened at the density of 1?10~6/cm~2 MNCs. It is feasible that the efficiency of isolating and harvesting ECs could be measured by the number of the first or the second passage ECs from every 10~6 MNCs. The induced ECs can act as seeding cells in bone tissue engineering in future.

Objective: To observe the clinical effect of integrated traditional and western medicine in treating chronic idiopathic thrombocytopenic purpura (CITP) elderly patients. Methods: Sixty patients were divided randomly into the treatment group (n=30) and the control group (n=30), the former was treated with danazol combining with prednisone and Chinese drugs, while the latter was treated with VCR. Results: The total effective rate of the treatment group was better than that of the control group, the difference between those two groups was significant (P

Objective To analyze the application effect of escitalopram in the patients with acute schizophrenia with depression and influence situation for the disease related blood indexes.Methods 84 patients with acute schizophrenia with depression in our hospital from February 2015 to August 2016 were selected as the study object, and they were randomly divided into control group(conventional treatment group)42 cases and observation group( conventional treatment and escitalopram group ) 42 cases, then the total effective rates, plasma free amino acids and stress hormones indexes before and after the treatment of two groups were compared.Results The total effective rates of observation group at different time after the treatment were all higher than those of control group, the difference was statistically significant (P<0.05), the plasma free amino acids after the treatment were all higher than those of control group, the difference was statistically significant (P<0.05), serum stress hormones were all lower than those of control group, the difference was statistically significant (P<0.05), the differences of two groups were all significant.Conclusion The application effect of escitalopram in the patients with acute schizophrenia with depression is better, and it also has active improvement role for the disease related blood indexes of patients.

The proliferation of MG63 cells under high glucose intervened with metformin was measured by CCK-8 assay.The activity of intracellular alkaline phosphatase (ALP) was detected by SFBC assay.The expression of related genes was detected by RT-PCR.Metformin promoted the proliferation of MG63 cells (P＜0.01),the activity of ALP was increased (P＜0.05).The expressions of collagen Ⅰ and osteocalcin were up-regulated while the expressions of matrix metalloproteinase (MMP)-1,MMP-2,MMP-3,and MMP-8 were down-regulated (P＜0.05).The results indicate that metformin may protect osteoblast,via promoting osteoblast proliferation,differentiation and mineralization,as well as by inhibiting degradation of osteoblast extracellular collagen matrix.

Objective: To establish the evaluation of the chemial quality standard of Radix Astragali according to their contents of saponin, flavonoid and polyscaccharide. Methods: HPLC ELS was used to determine astragaloside Ⅳ content. RP HPLC was used to determine 10 hydroxy 3,9 dimethoxypterocarpan content. Sulfuric acid phenol method was used to determine total polysaccharide content. Results: Astragaloside Ⅳ, total flavonoid, 10 hydroxy 3,9 dimethoxypterocarpan and total polysaccharide content of Astragalus mongmolicus Fisch. were 0.710? 0.030mg/g, 45?4.243mg/g, 19.955?0.813?g/g, 73.310?0.962mg/g, respectively; Astragaloside Ⅳ, total flavonoid, 10 hydroxy 3,9 dimethoxypeterocarpan and total polysaccharide content of Astragalus membranacens Fisch. were 1.186?0.024mg/g, 46?1.414mg/g,84.640?3.069?g/g, 48.800?1.245mg/g, respectively. Conclusion: The method was practical and provided a new idea to study the chemical quality standard of traditional Chinese drug.